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您的检索式：作者名="Fengying Sui"
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| 1 | 氯喹衍生物CQ11逆转乳腺癌多药耐药细胞株MCF/DOX对多柔比星的耐药性(英文)显示文摘Objective: To investigate the reversal effect of CQ11, a chloroquine derivative, on multidrug resistance (MDR) in doxorubicin (DOX)-resistant human breast carcinoma cell line MCF/DOX. Methods: Cells of a human breast cancer cell line, MCF, and its DOX-resistant variant, MCF/DOX, were cultivated with DOX and /or CQ11. The cytotoxicity of drugs in vitro was assayed by MTT method. The accumulation of DOX in these cells was detected by fluorescence spectrophotometer. Results: MCF/DOX cells were 119 times more resistant to DOX in comparison with MCF cells. After simultaneous treatment with CQ11 at the concentrations of 1.0, 2.5 and 5.0 μmol/L, the IC50 of DOX for MCF/DOX cells decreased from 3.1 ± 0.47 μmol/L to 0.58 ± 0.032, 0.19 ± 0.012 and 0.081 ± 0.015 μmol/L, respectively, thus, increasing the DOX sensitivity by 5.3-fold (P < 0.01), 16- fold (P < 0.01) and 38-fold (P < 0.01), respectively. In the accumulation assay of DOX, simultaneous incubation of MCF/DOX cells with CQ11 significantly increased the DOX accumulation in MCF/DOX cells. No such results were found in parental MCF cells. Conclusion: CQ11 had strong MDR reversal effect by enhancing intracellular DOX accumulation in MCF/DOX cells, indicating that CQ11 may be a promising MDR chemosensitivity. | Dalong Wu Shirong Ma Fengying Sui Chengwen Zhang Lixin Yin Huanzhang Lu | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，11： | 0 |
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