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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | High speed label-free detection by spinning disk micro-interferometry 显示文摘 | Manoj M Varma Halina D Inerowicz Fred E Regnier | 2004 | Biosensors and Bioelectronics2004，19，11： | 1 |
| 3 | Econ- omic evaluations of clinical pharmacy services: 2001-2005 显示文摘 | ALEXANDRA P FRED D HOTLMAN J M | 2008 | Pharmacotherapy2008，28，11： | 1 |
| 4 | Etionpathogenesis of acute pancretits显示文摘 | Suresh-Karne M D Ph D Fred S | 1999 | Surg Clin North Am1999，79，4： | 1 |
| 5 | Landscape features associated with lyme disease risk in a suburban residential environment 显示文摘 | Frank D H Fish D Fred H M | 1998 | Landscape Ecology1998，13，1： | 1 |
| 6 | Synthetic aperture radar显示文摘 | Doerry A W Fred M D | 2004 | Optics and Photonics News2004，15，11： | 1 |
| 7 | Single day, patient-initiated famciclovir therapy for recurrent genital herpes: A randomized, double-blind, placebo-controlled trial 显示文摘 | Fred Y Aoki Stephen Francisco D M el al | 2006 | Clin Infect Dis2006，42，： | 1 |
| 8 | Evaporative light scattering detector: toward a general molecular weight cutoff characterization of nanofiltration mem- branes显示文摘 | LI X F FRED M BART D | 2009 | Anal Chem2009，8，： | 1 |
| 9 | Exploration of Lower Cretaceous sands in the Leland Area,Alberta, using seismically derived rock properties显示文摘 | Carmen C D Fred M Rodney C | 2009 | First Break2009，27，11： | 1 |
| 10 | Rivaroxaban versus enoxaparin for thromboprophylaxis after total knee arthroplasty (RECORD4): a randomised trial显示文摘 | Alexander GG Turpie Michael R Lassen Bruce L Davidson Kenneth A Bauer Michael Gent Louis M Kwong Fred D Cushner Paul A Lotke Scott D Berkowitz Tiemo J Bandel Alice Benson Frank Misselwitz William D Fisher | 2009 | The Lancet2009，，9676： | 1 |
| 11 | An exploratory hazard-based analysis of highway incident duration 显示文摘 | NAM D FRED M | 2000 | Transportation Research Part A2000，34，2： | 1 |
| 12 | Vision system for defect imaging, detection, and characterization on a specular surface of a 3D object显示文摘 | DEINIS A FRED M CHIRISTOPHE D | 2002 | Image and Vision Computing2002，20，8： | 1 |
| 13 | Econ-omic evaluations of clinical pharmacy services:2001-2005显示文摘 | Alexandra P Fred D Hotlman J M | | 0，，： | 1 |
| 14 | Praziquantel efficacy against Schistosoma mansoni among HIV-1 infected and uninfected adults living in fishing villages along Lake Victoria, Northwest Tanzania显示文摘Background:Animal studies have demonstrated that functional immune responses,as determined by the levels of CD4+cell counts and anti-schistosome antibodies responses,determine the efficacy of praziquantel.Based on this evidence,it has been hypothesised that the immunodeficiency effects of the human immunodeficiency virus(HIV)-1 infection may affect the efficacy of praziquantel in co-infected human hosts.Thus,the present study assessed the efficacy of praziquantel by comparing parasitological cure rates and the reduction in infection intensity in HIV-1 seronegative individuals infected with S.mansoni and HIV-1 seropositive individuals co-infected with S.mansoni,following treatment with a single oral dose of praziquantel.Methods:This was a prospective longitudinal study which included,at baseline,555 S.mansoni infected adults aged 21-55 years,who were either co-infected or not with HIV-1 and who lived in fishing villages along Lake Victoria in Northwest Tanzania.These individuals were treated with a single oral dose of praziquantel(40 mg/kg)and,at 12 weeks,single stool samples were obtained and examined for S.mansoni eggs using the Kato-Katz technique.Finger prick and venous blood samples were collected for HIV-1 screening and CD4+cell quantification.Results:The parasitological cure rate did not differ significantly from the HIV-1 serostatus(P=0.12):among the co-infected individuals,the cure rate was 48.3%(14/29),and among the individuals infected only with S.mansoni,the cure rate was 62.6%(329/526).The egg reduction rate did not vary with the HIV-1 serostatus(P=0.22):77.22%for HIV-1 seronegative and 75%for HIV-1 seropositive individuals.The level of CD4^(+) cell counts(median 228 cells/μL:range 202-380 cells)did not influence the cure rate(P=0.23)or the reduction in the intensity of the infection(P=0.37).Conclusion:The HIV-1 infection per se or its moderate immunodeficiency effects,demonstrated by the range of CD4^(+) cell counts observed in co-infected individuals,did not affect praziquantel efficacy,as measured by the parasitological cure rate and the reduction in intensity of infection in the present study cohort. | Humphrey D Mazigo David W Dunne Safari M Kinung’hi Fred Nuwaha | 2014 | Infectious Diseases of Poverty2014，3，1： | 1 |
| 15 | Entrepreneurial learning and the growth process in SMEs 显示文摘 | Deakins D Fred M | 1998 | The aming Organization: An International Journal1998，5，3： | 1 |
| 16 | Immunogenic measles antigens expressed in plants: role as an edible vaccine for adults显示文摘 | Claude P M Fred F Benjamin D | 2003 | Vaccine2003，21，7： | 1 |
| 17 | The Genotoxic Potential of Electric and Magnetic Fields显示文摘 | JOYCE M FRED D CHARLES R | 1998 | Mutation Research1998，411，： | 1 |
| 18 | Rivaroxaban versus enoxaparin for thromboprophylaxis after total knee arthroplasty (RECORD4): a randomised trial显示文摘 | Alexander GG Turpie Michael R Lassen Bruce L Davidson Kenneth A Bauer Michael Gent Louis M Kwong Fred D Cushner Paul A Lotke Scott D Berkowitz Tiemo J Bandel Alice Benson Frank Misselwitz William D Fisher | 2009 | The Lancet2009，，9676： | 1 |
| 19 | Multiple horizontal gene transfers of ammonium transporters/ammonia permeases from prokaryotes to eukaryotes: toward a new functional and evolutionary classification显示文摘 | Tami M Fred D Franois L | 2011 | MBE Advance Access2011，29，1： | 1 |
| 20 | Integrated approach to teaching and testing in histology with real and virtual imaging显示文摘 | Heidger P M Fred D Daniel C | 2002 | Anat Rec2002，269，2： | 1 |
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