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| 1 | Reprogramming of a defense signaling pathway in rough lemon and sweet orange is a critical element of the early response to‘Candidatus Liberibacter asiaticus’显示文摘Huanglongbing(HLB)in citrus infected by Candidatus Liberibacter asiaticus(CLas)has caused tremendous losses to the citrus industry.No resistant genotypes have been identified in citrus species or close relatives.Among citrus varieties,rough lemon(Citrus jambhiri)has been considered tolerant due to its ability to produce a healthy flush of new growth after infection.The difference between tolerance and susceptibility is often defined by the speed and intensity of a plant’s response to a pathogen,especially early defense responses.RNA-seq data were collected from three biological replicates of CLas-and mock-inoculated rough lemon and sweet orange at week 0 and 7 following infection.Functional analysis of the differentially expressed genes(DEGs)indicated that genes involved in the mitogen activated protein kinase(MAPK)signaling pathway were highly upregulated in rough lemon.MAPK induces the transcription of WRKY and other transcription factors which potentially turn on multiple defense-related genes.A Subnetwork Enrichment Analysis further revealed different patterns of regulation of several functional categories,suggesting DEGs with different functions were subjected to reprogramming.In general,the amplitude of the expression of defense-related genes is much greater in rough lemon than in sweet orange.A quantitative disease resistance response may contribute to the durable tolerance level to HLB observed in rough lemon. | Qibin Yu Chunxian Chen Dongliang Du Ming Huang Jiqiang Yao Fahong Yu Ronald H Brlansky Frederick G.Gmitter Jr | 2017 | Horticulture Research2017，4，1： | 5 |
| 2 | 柑桔线虫抗性主基因座Tyr1的特异标记开发与遗传作图改进(英文)显示文摘本文利用先期从BAC文库获得的NBS-LRR类候选抗病基因克隆序列Pt8a和Pt9a,进一步开发与柑桔线虫抗性主效基因位点Tyr1连锁的分子标记。以Pt8a和Pt9a序列作探针,通过高密度克隆印迹杂交,从BAC文库筛选出200个以上的阳性克隆,以阳性克隆插入序列设计引物,对柑桔抗线虫材料和感线虫材料开展以PCR扩增为基础的集群分离分析,发现一部分克隆序列与柑桔线虫抗性主效基因位点Tyr1紧密连锁;再通过染色体步行测序,分别从3个克隆(7A4,4L17和29F20)获得3个完整的NBS-LRR类候选抗病基因序列。从此类序列开发更高特异性的分子标记,并在利用原有分子标记的基础上,对柑桔线虫抗性杂交后代群体(9145family)构建较高密度的遗传图谱;同时,将新开发的分子标记应用于柑桔衰退病抗性杂交后代群体(9401family),以初步估算柑桔线虫抗性主效基因位点Tyr1与柑桔衰退病抗性基因Ctv的遗传距离。本文利用先期从BAC文库获得的NBS-LRR类候选抗病基因克隆序列Pt8a和Pt9a,进一步开发与柑桔线虫抗性主效基因位点Tyr1连锁的分子标记。以Pt8a和Pt9a序列作探针,通过高密度克隆印迹杂交,从BAC文库筛选出200个以上的阳性克隆,以阳性克隆插入序列设计引物,对柑桔抗线虫材料和感线虫材料开展以PCR扩增为基础的集群分离分析,发现一部分克隆序列与柑桔线虫抗性主效基因位点Tyr1紧密连锁;再通过染色体步行测序,分别从3个克隆(7A4,4L17和29F20)获得3个完整的NBS-LRR类候选抗病基因序列。从此类序列开发更高特异性的分子标记,并在利用原有分子标记的基础上,对柑桔线虫抗性杂交后代群体(9145family)构建较高密度的遗传图谱;同时,将新开发的分子标记应用于柑桔衰退病抗性杂交后代群体(9401family),以初步估算柑桔线虫抗性主效基因位点Tyr1与柑桔衰退病抗性基因Ctv的遗传距离。 | 向旭 邓占鳌 郑启发 陈存贤 Frederick G.Gmitter Jr | 2009 | 分子植物育种2009，7，3： | 2 |
| 3 | Determining the Characteristic of the So- cially Conscious Consumer 显示文摘 | Webster Frederick E Jr | 1975 | Journal of Consumer Research1975，，2： | 1 |
| 4 | Eddy current offers a powerful tool for investigating residual stress and other metallurgical properties显示文摘 | Hong Chang Frederick C Schoenig Jr Jack A Soules | 1999 | Materials Evaluation1999，57，12： | 1 |
| 5 | Effect of TRA- 8 anti-death receptor 5 antibody in combination with chemotherapy in an ex vivo human ovarian cancer mode显示文摘 | Frederick PJ Kendrick JE Straughn JM Jr | 2009 | Int J Gynecol Cancer2009，19，5： | 1 |
| 6 | The Changing Role of Marketing in the Corporation显示文摘 | FREDERICK E WBESTER JR | 1992 | Reprinted from Journal of Marketing published by the American Marketing Association1992，56，： | 1 |
| 7 | Regres-sion rates study of mixed hybrid propellants显示文摘 | Frederick R A Jr Whitehead J J’Knox L R | 2007 | Journal ofPropulsion and Power2007，23，1： | 1 |
| 8 | A Monte Carlo study of IMRT beamlets in inhomogeneous media显示文摘 | Andrew O.Jones Indra J.Das Frederick L.Jones Jr | | 0，，03： | 1 |
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| 11 | KAP-1,a novel corepressor for the highly conserved KRAB repression domain显示文摘 | Friedman JR Fredericks WJ Jensen DE | | 0，，16： | 1 |
| 12 | Corporate Culture, Customer Orientation, and Innovativeness in Japanese Firms: A Quadrad Analysis 显示文摘 | Deshpande John U Farley Frederick Webster Jr | 1993 | Journal of Maketing1993，，57： | 1 |
| 13 | Preclinical stud-ies and clinical utilization of monoclonal antibodies in epithelial o-varian cancer显示文摘 | Frederick PJ Straughn JM Jr Alvarez RD | 2009 | Gynecol Oncol2009，113，3： | 1 |
| 14 | Organizational Culture and Marketing: Defining the Research Agenda 显示文摘 | ROHIT DESHPANDE FREDERICK E WEBSTER Jr | 1989 | Journal of Marketing1989，，53： | 1 |
| 15 | Effect of oxygenation level on yard trimmings composing rate, odor production, and compost quality in bench-scale reactors显示文摘 | Michel Jr Frederick C Reddy C A | 1998 | Compost Science & Utilization1998，6，4： | 1 |
| 16 | The Computer Scientist as ToolsmithlI显示文摘 | Brooks Frederick P Jr | 1996 | CACM1996，39，： | 1 |
| 17 | Composting rate,odor production,and compost quality in bench-scale reactors显示文摘 | Frederick C Michel Jr Reddy C A | 1998 | Compost Science and Utilization1998，6，4： | 1 |
| 18 | Generation of lymphokine-activated killer cell activity from human thymocytes 显示文摘 | Frederick JR Sidney HG | 1987 | J Immunol1987，139，： | 1 |
| 19 | Vitreoretinal manage- ment of traumatic dislocation of the crystalline lens 显示文摘 | Marcus DM Topping TM Frederick AR Jr | 1995 | Int Ophthalmol Clin1995，35，1： | 1 |
| 20 | A perspective on the evolution of marketing management显示文摘 | Frederick E Webster Jr | 2005 | Journal of Public Policy & Marketing2005，24，1： | 1 |
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