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| 1 | HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays显示文摘AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma. | Lucas Faria Abraho-Machado Alexandre Andrade dos Anjos Jácome Durval Renato Wohnrath José Sebastio dos Santos Estela Cristina Carneseca José Humberto Tavares Guerreiro Fregnani Cristovam Scapulatempo-Neto | 2013 | World Journal of Gastroenterology2013，19，38： | 12 |
| 2 | The accuracy of p16/Ki-67 and HPV test in the detection of CIN2/3 in women diagnosed with ASC-US or LSIL显示文摘 | Possati-Resende J C Fregnani J H Kerr L M | 2015 | PLos One2015，10，01： | 1 |
| 3 | Effect of adjuvant chemoradiotherapy on overall survival of gastric cancer patients submitted to D2 lymphadenectomy显示文摘 | Alexandre A. A. Jácome Durval R. Wohnrath Cristovam Scapulatempo Neto José Humberto T. G. Fregnani Ana Luiza Quinto Ant?nio T. T. Oliveira Vinícius L. Vazquez Gilberto Fava Edson Z. Martinez José S. Santos | 2013 | Gastric Cancer2013，，2： | 1 |
| 4 | Assessment of pelvic lymph node micrometastatic disease in stages IB and IIA of carcinoma of the uterine cervix 显示文摘 | Fregnani JH Latorre MR Novik PR | 2006 | Int J Gynecol Cancer2006，16，3： | 1 |
| 5 | Prognostic impli- cations of altered human epidermal growth factor receptors (HERs) in gastric carcinomas: HER2 and HER3 are predictors of poor outcome显示文摘 | Begnami MD Fukuda E Fregnani JH | 2011 | J Clin Oncol2011，29，22： | 1 |
| 6 | Comparison of bio- logical behavior between early - stage adenocarcinoma and squa-mous cell carcinoma of the uterine cervix显示文摘 | Fregnani J HTG Soares FA Novik PR | 2008 | European Journal of Obstetrics ~ amp Gynecology and Reproductive Biology2008，136，21： | 1 |
| 7 | Dbwn-regula- tion of PHLDA1 gene expression is associated with breast cancer progression显示文摘 | NAGAI MA FREGNANI JH NETTO MM | 2007 | Breast Cancer Res Treat2007，106，1： | 1 |
| 8 | Evaluation of gene amplification and protein expression of HER-2/neu in esophageal squamous cell carcinoma using fluorescence in situ hybridization ( FISH ) and immunohistochemistry 显示文摘 | Sato-Kuwabara Y Neves JI Fregnani JH | 2009 | BMC Cancer2009，9，6： | 1 |
| 9 | Synde-can-1(CD138)and Ki-67expression in odontogenic cystic le-sions显示文摘 | NADALIN MR FREGNANI ER SILVA-SOUSA YT | 2011 | Braz Dent J2011，22，3： | 1 |
| 10 | Evaluation of cell cycle protein expression in gastric cancer: cyclin B1 expression and its prognostic implication显示文摘 | Begnami MD Fregnani J H Nonogaki S | 2010 | Hum Pathol2010，41，8： | 1 |
| 11 | Prognostic implica- tions of altered human epidermal growth factor receptors (HERs) in gastric carcinomas:HER2 and HER3 are predictors of poor outcome显示文摘 | Begnami MD Fukuda E Fregnani JH | 2011 | J Clin Oneol2011，29，22： | 1 |
| 12 | Evaluation of prognostic factors in stage ⅡA breast tumors and their correlation with mortality risk显示文摘 | Carvalho ST Stiepcich MM Fregnani JH | | 0，，： | 1 |
| 13 | Down-regulation of PHLDA! gene expression is associated with breast cancer progression显示文摘 | Nagai MA Fregnani JH Netto MM | 2007 | Breast Cancer Res Treat2007，106，1： | 1 |
| 14 | Prognostic implica- tions of altered human epidermal growth factor receptors (HERs) in gastric carcinomas: HER2 and HER3 are predictors of poor outcome显示文摘 | Begnami MD Fukuda E Fregnani JH | 2011 | J Clin Oncol2011，29，22： | 1 |
| 15 | Prognostic im- plications of altered human eoidermal growth factor receptors (HERs) in gastric carcinomas; HER2 and HER3 are predic- tors of poor outcome显示文摘 | Begnami M D Fukuda E Fregnani J H | 2011 | J Clin Oncol2011，29，22： | 1 |
| 16 | Characterization of p16 and E6 HPV-related proteins in uterine cervix high-grade le- sions of patients treated by canizatian with large loop excision 显示文摘 | Roncaglia MT Fregnani JH Tacla M | 2013 | Oncol Lett2013，6，1： | 1 |
| 17 | Evaluation of cell cycle protein expression in gastric cancer:Cyclin B1 expression and its prognostic implication显示文摘 | Begnami M D Fregnani J H Nonogaki S | 2010 | Hum Pathol2010，41，8： | 1 |
| 18 | Evaluation of gene amplification and protein expression of HER-2/neu in esophageal squamous cell carcinoma using Fluorescence in situ Hybridization (FISH) and immunohistochemistry显示文摘 | Sato-Kuwabara Y Neves JI Fregnani JH | 2009 | BMC Cancer2009，9，： | 1 |
| 19 | Digital photogra- phy in skin cancer screening by mobile units in remote areas of Brazil 显示文摘 | Silveira CE Silva TB Fregnani JH | 2014 | BMC Dermatol2014，2414，： | 1 |
| 20 | Clinicopath-ological study and treatment outcomes of 121 cases of ameloblas-tomas显示文摘 | Fregnani ER da Cruz Perez DE de Almeida OP | 2010 | Int J Oral Maxillofac Surg2010，39，2： | 1 |
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