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| 1 | HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays显示文摘AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma. | Lucas Faria Abraho-Machado Alexandre Andrade dos Anjos Jácome Durval Renato Wohnrath José Sebastio dos Santos Estela Cristina Carneseca José Humberto Tavares Guerreiro Fregnani Cristovam Scapulatempo-Neto | 2013 | World Journal of Gastroenterology2013，19，38： | 12 |
| 2 | Polymorphisms of DNA repair genes XRCC1 and XRCC3, interaction with environmental exposure and risk of chronic gastritis and gastric cancer显示文摘AIM: To evaluate the association between polymorphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3Thr241Met and the risk for chronic gastritis and gastric cancer, in a Southeastern Brazilian population.METHODS: Genotyping by PCR-RFLP was carried out on 202 patients with chronic gastritis (CG) and 160 patients with gastric cancer (GC), matched to 202 (C1) and 150(C2) controls, respectively.RESULTS: No differences were observed among the studied grou ps with regard to the genotype distribution of XRCC1 codons 194 and 399 and of XRCC3 codon 241. However, the combined analyses of the three variant alleles (194Trp, 399Gln and 241Met) showed an increased risk for chronic gastritis when compared to the GC group. Moreover, an interaction between the polymorphic alleles and demographic and environmental factors was observed in the CG and GC groups. XRCC1 194Trp was associated with smoking in the CG group,while the variant alleles XRCC1 399Gln and XRCC3241Met were related with gender, smoking, drinking and H pylori infection in the CG and GC groups.CONCLUSION: Our results showed no evidence of a rela-tionship between the polymorphisms XRCC1Arg194Trp and Arg399Gln and XRCC3 Thr241Met and the risk of chronic gastritis and gastric cancer in the Brazilian population, but the combined effect of these variants may interact to increase the risk for chronic gastritis,considered a premalignant lesion. Our data also indicate a gene-environment interaction in the susceptibility to chronic gastritis and gastric cancer. | Márcia Cristina Duarte Jucimara Colombo Andrea Regina Baptista Rossit Alaor Caetano Aldenis Albaneze Borim Durval Wornrath Ana Elizabete Silva | 2005 | World Journal of Gastroenterology2005，11，42： | 11 |
| 3 | GSTT1,GSTM1 and CYP2E1 genetic polymorphisms in gastric cancer and chronic gastritis in a Brazilian population显示文摘MIM:To test the hypothesis that,in the Southeastern Brazilian population,the GSTT1,GSTM1 and CYP2E1 polymorphisms and putative risk factors are associated with an increased risk for gastric cancer.METHODS:We conducted a study on 100 cases of gastric cancer(GC),100 cases of chronic gastritis(CG),and 150 controls(C).Deletion of the GSTT1 and GSTM1 genes was assessed by multiplex PCR.CYP2E1/PsА genotyping was performed using a PCR-RFLP assay.RESULTS:No relationship between GSTT1/GSTM1 deletion and the c1/c2 genotype of CYP2E1 was observed among the three groups.However,a significant difference between CG and C was observde,due to a greater number of GSTT1/GSTM1 positive genotypes in the CG group.The GSTT1 null genotype occurred more frequently in Negroid subjicts,and the GSTM1 null genotype was observed mainly in individuals with chronic gastritis infected with H pylori.CONCLUSION:Our findings indecate that there is no obvious relationship between the GSTT1,GSTM1 and CYP2E1 polymorphisms and gastric cancer. | Jucimara Colombo Ana Elizabete Silva Andréa Regina Baptista Rossit Alaor Caetano Aldenis Albaneze Borim Durval Wohnrath | 2004 | World Journal of Gastroenterology2004，10，9： | 11 |
| 4 | AngiotensinⅡor epinephrine hemodynamic and metabolic responses in the liver of L-NAME induced hypertension and spontaneous hypertensive rats显示文摘AIM To study hepatic vasoconstriction and glucose release induced by angiotensin(Ang)Ⅱ or Epi in rats with pharmacological hypertension and spontaneously hypertensive rat(SHR).METHODS Isolated liver perfusion was performed following portal vein and vena cava cannulation; AngⅡ or epinephrine(Epi) was injected in bolus and portal pressure monitored; glucose release was measured in perfusate aliquots. RESULTS The portal hypertensive response(PHR) and the glucose release induced by AngⅡ of L-NAME were similar to normal rats(WIS). On the other hand, the PHR inducedby Epi in L-NAME was higher whereas the glucose release was lower compared to WIS. Despite the similar glycogen content, glucose release induced by AngⅡ was lower in SHR compared to Wistar-Kyoto rats although both PHR and glucose release induced by Epi in were similar. CONCLUSION AngⅡ and Epi responses are altered in different ways in these hypertension models. Our results suggest that inhibition of NO production seems to be involved in the hepatic effects induced by Epi but not by AngⅡ; the diminished glucose release induced by AngⅡ in SHR is not related to glycogen content. | Debora Conte Kimura Marcia Regina Nagaoka Durval Rosa Borges Maria Kouyoumdjian | 2017 | World Journal of Hepatology2017，9，17： | 4 |
| 5 | Prognostic value of epidermal growth factor receptors in gastric cancer: a survival analysis by Weibull model incorporating long-term survivors显示文摘 | Alexandre Andrade Anjos Jácome Durval R. Wohnrath Cristovam Scapulatempo Neto Estela C. Carneseca Sérgio V. Serrano Luciano Souza Viana Jo?o S. Nunes Edson Z. Martinez José Sebasti?o Santos | 2014 | Gastric Cancer2014，，1： | 2 |
| 6 | Effect of adjuvant chemoradiotherapy on overall survival of gastric cancer patients submitted to D2 lymphadenectomy显示文摘 | Alexandre A. A. Jácome Durval R. Wohnrath Cristovam Scapulatempo Neto José Humberto T. G. Fregnani Ana Luiza Quinto Ant?nio T. T. Oliveira Vinícius L. Vazquez Gilberto Fava Edson Z. Martinez José S. Santos | 2013 | Gastric Cancer2013，，2： | 1 |
| 7 | Using a minimum quantity of lubricant (MQL) and a diamond coated tool in the drilling of aluminum-silicon alloys显示文摘 | DURVAL U ANSELMO E GILBERTO W | 2002 | Journal of Materials Processing Technology2002，122，： | 1 |
| 8 | Investigation of the ZFY gene in XX true hermaphroditism and Swyer syndrome显示文摘 | Durval Damiani Ana Elisa C. Billerbeck Anna Carla K. Goldberg Nuvarte Setian Marc Fellous Jorge Kalil | 1990 | Human Genetics1990，，1： | 1 |
| 9 | Adaptive fuzzy controller for efficiency optimization of induction motors显示文摘 | Durval de Almeida Souza Wilson C P de Aragal Filho Gilberto Costa Drumond Sousa | 2007 | IEEE Trans on Industrial Electronics2007，54，4： | 1 |
| 10 | Using a minimum quantity of lubricant (MQL) and a diamond coated tool in the drilling of alumi- num- silicon alloys 显示文摘 | Durval U B Diniz A E Gilberto W A | 2002 | Journal of Materials Processing Technology2002，122，1： | 1 |
| 11 | A comparison of three radionuclide myocardial perfusion tracers in clinical practice: the ROBUST study显示文摘 | Akhil Kapur Katherine A. Latus Glyn Davies Rhanju T. Dhawan Sian Eastick Peter H. Jarritt George Roussakis Melanie C. Young Constantinos Anagnostopoulos Jimmy Bomanji Durval C. Costa Dudley J. Pennell Elizabeth M. Prvulovich Peter J. Ell Richard S. Underw | 2002 | European Journal of Nuclear Medicine and Molecular Imaging2002，，12： | 1 |
| 12 | A comparison of three radionuclide myocardial perfusion tracers in clinical practice: the ROBUST study显示文摘 | Akhil Kapur Katherine A. Latus Glyn Davies Rhanju T. Dhawan Sian Eastick Peter H. Jarritt George Roussakis Melanie C. Young Constantinos Anagnostopoulos Jimmy Bomanji Durval C. Costa Dudley J. Pennell Elizabeth M. Prvulovich Peter J. Ell Richard S. Underw | 2002 | European Journal of Nuclear Medicine and Molecular Imaging2002，，12： | 1 |
| 13 | Hyperlipidemic acute panere- atitis : a possible role of antiretroviral therapy with entecavir 显示文摘 | Durval A Zamidei L Bettocchi D | 2011 | Mi- nerva Anestesio12011，77，10： | 1 |
| 14 | Adaptive Fuzzy Controller for Efficiency Optimization of Induction Motors显示文摘 | Durval de Almeida Souza Wilson C P de Aragao Filho Gilberto Costa Drumond Sousa | 2007 | IEEE Transactions on Industrial Electronics2007，54，4： | 1 |
| 15 | Using a minimum quantity of lubricant (MQL) and a diamond coated tool in the drilling of aluminumsilicon alloys 显示文摘 | DURVAL U B DINIZ A E GILBERTO W A | 2002 | Journal of Materials Processing Technology2002，122，1： | 1 |
| 16 | Using a minimum quantity of lubricant (MQL) and a diamond coated tool in the drilling of aluminum-silicon alloys 显示文摘 | D U Durval | 2002 | Journal of Materials Processing Technology2002，122，： | 1 |
| 17 | SPECT imaging of the dopamine transporter in juvenile-onset dystonia 显示文摘 | O'Sullivan John D Costa Durval C | 2001 | Neurology2001，56，2： | 1 |
| 18 | Properties and flux pinning of Cu-Nb superconductors with nanometric-scale pinning centers 显示文摘 | Rodrigues Jr Durval Rodrigues | 2005 | IEEE Transactions on Applied Superconductivity2005，15，2： | 1 |
| 19 | Considerations about winglet design 显示文摘 | Bento Mattos E Antonini Maeedo E Durval Silva Filho E | 2003 | 2003，，： | 1 |
| 20 | Adaptive Fuzzy Controller for Efficiency Optimization of Induction Motors 显示文摘 | Durval de Almeida Souza Wilson C P de Aragao Filho Gil- berto Costa Drumond Sousa | 2007 | IEEE Trans- actions on Industry Electronics2007，54，4： | 1 |
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