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| 1 | Deletion of a Non-Catalytic Region Increases the Enzymatic Activity of a β-Agarase from Flammeovirga sp. MY04显示文摘A Glycoside hydrolase(GH) typically contains one catalytic module and varied non-catalytic regions(NCRs). However, effects of the NCRs to the catalytic modules remain mostly unclear except the carbohydrate-binding modules(CBMs). Aga G4 is a GH16 endo-β-agarase of the agarolytic marine bacterium Flammeovirga sp. MY04. The enzyme consists of an extra sugar-binding peptide within the catalytic module, with no predictable CBMs but function-unknown sequences in the NCR, which is a new characteristic of agarase sequences. In this study, we deleted the NCR sequence, a 140-amino acid peptide at the C-terminus and expressed the truncated gene, aga G4-T140, in Escherichia coli. After purification and refolding, the truncated agarase r Aga G4-T140 retained the same catalytic temperature and p H value as r Aga G4. Using combined fluorescent labeling, HPLC and MS/MS techniques, we identified the end-products of agarose degradation by r Aga G4-T140 as neoagarotetraose and neoagarohexaose, with a final molar ratio of 1.53:1 and a conversion ratio of approximately 70%, which were similar to those of r Aga G4. However, the truncated agarase r Aga G4-T140 markedly decreased in protein solubility by 15 times and increased in enzymatic activities by 35 times. The oligosaccharide production of r Aga G4-T140 was approximately 25 times the weight of that produced by equimolar r Aga G4. This study provides some insights into the influences of NCR on the biochemical characteristics of agarase Aga G4 and implies some new strategies to improve the properties of a GH enzyme. | HAN Wenjun GU Jingyan LIU Huihui LI Fuchuan WU Zhihong LI Yuezhong | 2015 | Journal of Ocean University of China2015，14，5： | 2 |
| 2 | The potential molecular targets of marine sulfated poly-mannuroguluronate interfering HIV-1 entry显示文摘 | Geng Meiyu Li Fuchuan Xin Xianliang | 2003 | Antiviral Research2003，59，： | 1 |
| 3 | Sulfated polymannuroguluronate, a novel anti-acquired immune deficiency syndrome (AIDS) drug candidate, targeting CD4 in lymphocytes显示文摘 | Benchun Miao Meiyu Geng Jing Li Fuchuan Li Haixia Chen Huashi Guan Jian Ding | 2004 | Biochemical Pharmacology2004，，4： | 1 |
| 4 | Choquet typefuzzy complex-valued integral and its application in classi-fication 显示文摘 | Ma Shengquan Chen Fuchuan Zhao Zhiqing | 2012 | Fuzzy Engineering and Operations Research2012，147，： | 1 |
| 5 | Bandpass transmission spectra of a whisperinggallery microcavity coupled to an ultrathin fiber显示文摘Tapered fibers with diameters ranging from 1 to 4 μm are widely used to excite the whispering-gallery(WG)modes of microcavities. Typically, the transmission spectrum of a WG cavity coupled to a waveguide around a resonance assumes a Lorentzian dip morphology due to resonant absorption of the light within the cavity. In this paper, we demonstrate that the transmission spectra of a WG cavity coupled with an ultrathin fiber(500–700 nm)may exhibit both Lorentzian dips and peaks, depending on the gap between the fiber and the microcavity. By considering the large scattering loss of off-resonant light from the fiber within the coupling region, this phenomenon can be attributed to partially resonant light bypassing the lossy scattering region via WG modes, allowing it to be coupled both to and from the cavity, then manifesting as Lorentzian peaks within the transmission spectra.This implies the system could be implemented within a bandpass filter framework. | FUCHUAN LEI RAFINO M.J.MURPHY JONATHAN M.WARD YONG YANG SíLE NIC CHORMAIC | 2017 | Photonics Research2017，5，4： | 1 |
| 6 | Reducedorder H∞ filtering for linear systems with Markovian jump parameters 显示文摘 | Sun Fuchuan Liu Huaping He Kezheng | 2005 | Systems and Control Letters2005，54，8： | 1 |
| 7 | The hip stress level analysis for human routine activities显示文摘 | WANG Xishi WANG Tianying JIANG Fuchuan | 2005 | Biomedical Engineering2005，17，3： | 1 |
| 8 | Comparative patterns of reading eye movement in Chinese and English 显示文摘 | FUCHUAN S MORITA M LAWRENCE W S | 1985 | Perception & Psychophysics1985，37，： | 1 |
| 9 | Higher vaspin levels in subjects with obesity and type 2 diabetes mellitus: A meta-analysis显示文摘 | Rennan Feng Yanchuan Li Cheng Wang Chao Luo Liyan Liu Fuchuan Chuo Qiang Li Changhao Sun | 2014 | Diabetes Research and Clinical Practice2014，，1： | 1 |
| 10 | Dynamics and entanglement of a membrane-in-the-middle optomechanical system in the extremely-large-amplitude regime显示文摘The study of optomechanical systems has attracted much attention, most of which are concentrated in the physics in the smallamplitude regime. While in this article, we focus on optomechanics in the extremely-large-amplitude regime and consider both classical and quantum dynamics. Firstly, we study classical dynamics in a membrane-in-the-middle optomechanical system in which a partially reflecting and flexible membrane is suspended inside an optical cavity. We show that the membrane can present self-sustained oscillations with limit cycles in the shape of sawtooth-edged ellipses and exhibit dynamical multistability. Then, we study the dynamics of the quantum fluctuations around the classical orbits. By using the logarithmic negativity, we calculate the evolution of the quantum entanglement between the optical cavity mode and the membrane during the mechanical oscillation. We show that there is some synchronism between the classical dynamical process and the evolution of the quantum entanglement. | Ming Gao FuChuan Lei ChunGuang Du GuiLu Long | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，1： | 1 |
| 11 | Very large group delay in VHF band using coupled high temperature superconducting resonators显示文摘Storing a very high frequency(VHF)band(30–300 MHz)electromagnetic wave has many potential applications,such as phase modulation,buffering,and radio frequency memory.It can be effectively achieved by applying coupled resonator-based electromagnetically induced transparency(EIT)due to its slow light effect.However,the wavelength in the VHF band is too long to design resonators,and the group delay is limited by the high resistive loss of metal.The practical application of EIT in the VHF band is still a big challenge.In this work,we propose and experimentally demonstrate EIT response in a high-temperature superconducting(HTS)microwave circuit with coupled-resonator-induced transparency.The chip size of the HTS circuit is only 34 mm×20 mm with a very low transparency frequency of 198.55 MHz.In addition,we implement very large group delay higher than 12.3μs and 16.2μs with working temperatures of 65 K and 50 K separately,which is much longer than the previous reported works on slow wave.The fabricated circuit is planar with working temperature about 65 K,and thus can be easily integrated into other microwave devices under the cryogenic conditions provided by a commercial portable Stirling cryocooler.Our proposed method paves a way for studying EIT in the microwave region due to the high quality factor of the HTS resonator,which has great potential use for radio-frequency memory in the future. | TIANNING ZHENG BIN WEI FUCHUAN LEI BISONG CAO | 2021 | Photonics Research2021，9，10： | 0 |
| 12 | 中国北方的室外热舒适模型:现状、特点与应用显示文摘室外空间是人们进行室外活动的主要场所,在日常生活中扮演着重要的角色。《民用建筑热工设计规范》将北方定义为寒冷地区和严寒地区。在北方,室外空间的使用很大程度上取决于热舒适性。截止到今日,全球不同气候区的研究者们针对热舒适问题提出了过百种不同的热舒适模型,不同的热舒适模型的适应范围不同,在研究中需要结合研究目标选择合适的模型。本文回顾了近十年发表的关于中国北方地区的室外热舒适研究,回顾了现有的室外热舒适性模型的现状、特点与应用,并总结了每种模型的优缺点,希望能为室外热舒适研究研究者在今后的研究中选择适合中国北方地区的模型提供参考。 | 余海铭 周梦园 Wang Xisheng He Kun Guo Weiping Wang Fuchuan | 2021 | 陕西建筑2021，，1： | 0 |
| 13 | Blue band nonlinear optics and photodarkening in silica microdevices显示文摘There are extensive studies to date on optical nonlinearities in microcavities at the near and mid-IR wavelengths.Pushing this research into the visible region is equally valuable.Here,we demonstrate a directly pumped,blue band Kerr frequency comb and stimulated Raman scattering(SRS)at 462 nm in a silica nanofiber-coupled whispering gallery microcavity system.Notably,due to the high optical intensities achieved,photodarkening is unavoidable and can quickly degrade the optical quality of both the coupling optical nanofiber and the microcavity,even at very low pump powers.Nonetheless,stable hyperparametric oscillation and SRS are demonstrated in the presence of photodarkening by taking advantage of in-situ thermal bleaching.This work highlights the challenges of silica-based,short wavelength nonlinear optics in high-quality,small mode volume devices and gives an effective method to overcome this apparent limitation,thus providing a baseline for optics research in the blue region for any optical devices fabricated from silica. | KE TIAN JIBO YU FUCHUAN LEI JONATHAN WARD ANGZHEN LI PENGFEI WANG SÍLE NIC CHORMAIC | 2022 | Photonics Research2022，10，9： | 0 |
| 14 | Localization and Recognition of Single Particle Image in Microscopy Micrographs Based on Region Based Convolutional Neural Networks显示文摘 | Fang Zheng FuChuan Ni Liang Zhao | 2018 | 国际计算机前沿大会会议论文集2018，，1： | 0 |
| 15 | PTN and IP-Based Mobile Backhaul显示文摘Packet Transport Networks (PTN) combine the advantages of packet technology and Synchronous Digital Hierarchy (SDH) technology.Taking the packet switch as its core, PTN has a high statistical multiplexing ability, which allows it to become a more efficient packet transfer service.Its strong Operation Administration and Maintenance (OAM)-similar to SDH and carrier-class security protection services-ensures efficient mobile backhaul business management and transmission quality.MPLS Transport Profile (MPLS-TP) packet transport network eliminates connectionless features, such as Penultimate Hop Popping (PHP), label merge and Equal-cost multi-path (ECMP), and is enhanced in terms of OAM, protection, and synchronization.This is ideally suited for carrying IP-based mobile backhaul services and key account services.PTN, original Multi-Service Transport Platform (MSTP), Metro Ethernet, and IP over Wave Division Multiplexing (WDM)/Optical Transport Network (OTN) of organic complexes, contribute to an 'all IP' era of telecommunications services. | Cong Kai Zhao Fuchuan | 2010 | ZTE Communications2010，8，3： | 0 |
| 16 | The first mitogenome of Lauraceae(Cinnamomum chekiangense)显示文摘There are three distinct genetic systems in higher plants,the dominant nuclear genome and the semi-autonomous organelle genomes(plastids and mitochondria).In contrast to the conserved plastid genome(plastome),the plant mitochondrial genome(mitogenome)is characterized by an intriguing“evolutionary paradox”distinguished by a remarkably low mutation rate but with a significantly high rearrangement rate(Palmer and Herbon,1988;Lai et al.,2022). | Changwei Bi Ning Sun Fuchuan Han Kewang Xu Yong Yang David K.Ferguson | 2024 | Plant Diversity2024，46，1： | 0 |
| 17 | Functional Cure of Chronic Hepatitis B with Antiviral Treatment in Children having High-level Viremia and Normal or Mildly Elevated Serum Aminotransferase显示文摘Background and Aims:There is a lack of data supporting the notion that antiviral treatments can beneft children with chronic hepatitis B(CHB)having high viremia and normal or mildly elevated serum alanine aminotransferase(ALT)levels.We aimed t analyze the efficacy of antiviral treatments in children with CHB and explore the factors associated with functional cure.Methods:Forty-eight children with CHB having high viremia and normal or mildly elevated serum ALT levels were screened in this real-world study.Thirty-two children received either interferon-alpha(IFN-α)monother-apy,IFN-a therapy with a nucleoside analog(NA)add-on,or IFN-α and NA combination therapy.The 16 children in the control group did not receive antiviral treatment.All 48 chil-dren were available for follow-up assessments for the entire 36-month study period.We identified a functional cure with respect to hepatitis B virus(HBV)DNA loss,loss/seroconver-sion of circulating hepatitis B e antigen(HBeAg),and loss of hepatitis B surface antigen(HBsAg)with or without serocon-version.Cox regression analysis was employed to evaluate the factors that may have influenced the functional cure.Re-sults:After 36 months,the cumulative functional cure rate was 56.25%(18/32)in the treated group and 0%(0/16)in the control group(p<0.001).In the treated group,the serum HBV DNA levels declined rapidly at the end of a 6-month visit and the cured children achieved a loss rate of 100%(18/18)within 16 months of beginning treatment,compared with 64.29%(9/14)of the uncured children(p<0.001).The rates of HBeAg seroconversion were significantly higher among the cured children than among the uncured children(p<0.001).All 16 children in the control group maintained high levels of serum HBV DNA and were positive for both serum HBeAg and HBsAg during the entire 36 months of the study period.Func tional cure was associated with younger ages(1-6 vs.7-14 years,p=0.013),CD8^(+)T lymphocyte counts(p=0.013),and B lymphocyte counts(p=0.003).No serious adverse events were observed.Conclusions:Antiviral treatment achieved a functional cure of CHB in a high proportion of children having high-level viremia and normal or mildly elevated ALT levels.Younger age and high peripheral lymphocyte counts were as sociated with this functional cure. | Jing Li Peiyao Fan Zhiqiang Xu Yi Dong Fuchuan Wang Weiguo Hong Jinfang Zhao Yinjie Gao Jianguo Yan Lili Cao Chao Zhang Shishu Zhu Fu-Sheng Wang Min Zhang | 2023 | Journal of Clinical and Translational Hepatology2023，11，5： | 0 |
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