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4篇 您的检索式:作者名="G.F.Visser"
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1Phased,chromosome-scale genome assemblies of tetraploid potato reveal a complex genome,transcriptome,and predicted proteome landscape underpinning genetic diversity显示文摘Cultivated potato is a clonally propagated autotetraploid species with a highly heterogeneous genome.Phased assemblies of six cultivars including two chromosome-scale phased genome assemblies revealed extensive allelic diversity,including altered coding and transcript sequences,preferential allele expression,and structural variation that collectively result in a highly complex transcriptome and predicted proteome,which are distributed across the homologous chromosomes.Wild species contribute to the extensive allelic diversity in tetraploid cultivars,demonstrating ancestral introgressions predating modern breeding efforts.As a clonally propagated autotetraploid that undergoes limited meiosis,dysfunctional and deleterious alleles are not purged in tetraploid potato.Nearly a quarter of the loci bore mutations are predicted to have a high negative impact on protein function,complicating breeder’s efforts to reduce genetic load.The StCDF1 locus controls maturity,and analysis of six tetraploid genomes revealed that 12 allelic variants of StCDF1 are correlated with maturity in a dosage-dependent manner.Knowledge of the complexity of the tetraploid potato genome with its rampant structural variation and embedded deleterious and dysfunctional alleles will be key not only to implementing precision breeding of tetraploid cultivars but also to the construction of homozygous,diploid potato germplasm containing favorable alleles to capitalize on heterosis in F1 hybrids.Genevieve Hoopes Xiaoxi Meng John P.Hamilton Sai Reddy Achakkagari Fernanda de Alves Freitas Guesdes Marie E.Bolger Joseph J.Coombs Danny Esselink Natalie R.Kaiser Linda Kodde Maria Kyriakidou Brian Lavrijssen Natascha van Lieshout Rachel Shereda Heather K.Tuttle Brieanne Vaillancourt Joshua C.Wood Jan Mde Boer Nolan Bornowski Peter Bourke David Douches Herman Jvan Eck Dave Ellis Max J.Feldman Kyle M.Gardner Johannes C.P.Hopman Jiming Jiang Walter S.De Jong Joseph C.Kuhl Richard G.Novy Stan Oome Vidyasagar Sathuvalli Ek Han Tan Remco A.Ursum M.Isabel Vales Kelly Vining Richard G.F.Visser Jack Vossen G.Craig Yencho Noelle L.Anglin Christian W.B.Bachem Jeffrey B.Endelman Laura M.Shannon Martina V.Stromvik Helen H.Tai Bjorn Usadel C.Robin Buell Richard Finkers 2022Molecular Plant2022,15,3:4
2利用GBSSI和SBD作为“锚”将荧光素酶定位到淀粉粒中效果的比较显示文摘利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(GBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUC)定位到淀粉粒中.并对GBSSI/LUC重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中GBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了分析.结果表明,GBSSI/ LUC重组蛋白质在马铃薯淀粉粒中获得了特异性表达,但与以微生物的淀粉粒结合结构域(SBD) 作为“锚”的SBD/LUC重组蛋白质相比较,它与淀粉粒的亲和性以及荧光素酶的活性均下降.由此认为,在淀粉粒形成过程中,利用SBD作为“锚”将荧光素酶蛋白质定位到淀粉粒中的效果要优于GBSSI.季勤 Jean-Paul Vincken Krit Raemakers Luc C.J.M.Suurs Richard G.F.Visser 2006自然科学进展2006,16,6:4
3Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development显示文摘Christian W.B.Bachem Rutger S.Hoeven Steef M.Bruijn DickVreugdenhil MarcZabeau Richard G.F.Visser 2003The Plant Journal2003,,5:2
4Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development显示文摘Christian W.B.Bachem Rutger S.Hoeven Steef M.Bruijn DickVreugdenhil MarcZabeau Richard G.F.Visser 2003The Plant Journal2003,,5:1
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