3篇
您的检索式：作者名="G.Johnston"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Tension and Resolution: Dynamic, Evolving Populations of Organelle Genomes within Plant Cells显示文摘Mitochondria and plastids form dynamic, evolving populations physically embedded in the fluctuating environment of the plant cell. Their evolutionary heritage has shaped how the cell controls the genetic structure and the physical behavior of its organelle populations. While the specific genes involved in these processes are gradually being revealed, the governing principles underlying this controlled behavior remain poorly understood. As the genetic and physical dynamics of these organelles are central to bioenergetic performance and plant physiology, this challenges both fundamental biology and strategies to engineer better-performing plants. This article reviews current knowledge of the physical and genetic behavior of mitochondria and chloroplasts in plant cells. An overarching hypothesis is proposed whereby organelles face a tension between genetic robustness and individual control and responsiveness, and different species resolve this tension in different ways. As plants are immobile and thus subject to fluctuating environments, their organelles are proposed to favor individual responsiveness, sacrificing genetic robustness. Several notable features of plant organelles, including large genomes, mtDNA recombination, fragmented organelles, and plastid/mitochondrial differences may potentially be explained by this hypothesis. Finally, the ways that quantitative and systems biology can help shed light on the plethora of open questions in this field are highlighted. | lain G.Johnston | 2019 | Molecular Plant2019，12，6： | 1 |
| 2 | Erythropoietin Receptor Expression in Non‐Small Cell Lung Carcinoma: A Question of Antibody Specificity显示文摘 | W. MarkBrown PerryMaxwell Alastair N.J.Graham AnitaYakkundi Elaine A.Dunlop ZhanzhongShi Patrick G.Johnston Terence R.J.Lappin | 2009 | STEM CELLS2009，，3： | 1 |
| 3 | Bioproduced Proteins On Demand(Bio-POD)in hydrogels using Pichia pastoris显示文摘Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation capacity.As a result,these systems are not easily portable or reusable for on-demand protein production applications.In this study,we employ Bioproduced Proteins On Demand(Bio-POD),a F127-bisurethane methacrylate hydrogel-based technique that immobilizes engineered Pichia pastoris for preservable,on-demand production and secretion of medium-and high-molecular weight proteins(in this case,SEAP,α-amylase,and anti-HER2).The gel samples containing encapsulated-yeast demonstrated sustained protein production and exhibited productivity immediately after lyophilization and rehydration.The hydrogel platform described here is the first hydrogel immobilization using a P.pastoris system to produce recombinant proteins of this breadth.These results highlight the potential of this formulation to establish a cost-effective bioprocessing strategy for on-demand protein production. | Shuo-Fu Yuan Sierra M.Brooks Annalee W.Nguyen Wen-Ling Lin Trevor G.Johnston Jennifer A.Maynard Alshakim Nelson Hal S.Alper | 2021 | Bioactive Materials2021，6，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员