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| 1 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020,13,9: | 14 |
| 2 | Oil & Gas Drilling Technology in China:Past and Present显示文摘Drilling is a prerequisite for oil and gas exploration. As one of the core upstream business of oil and gas exploration and development, it mainly includes | Su Yinao Huang Hongchun Zhou Yuhui Gao Wenkai Zeng Qiang | 2009 | China Oil & Gas2009,16,4: | 9 |
| 3 | Gapless indica rice genome reveals synergistic contributions of active transposable elements and segmental duplications to rice genome evolution显示文摘The ultimate goal of genome assembly is a high-accuracy gapless genome.Here,we report a new assembly pipeline that is used to produce a gapless genome for the indica rice cultivar Minghui 63.The resulting 397.71-Mb final assembly is composed of 12 contigs with a contig N50 size of 31.93 Mb.Each chromosome is represented by a single contig and the genomic sequences of all chromosomes are gapless.Quality evaluation of this gapless genome assembly showed that gene regions in our assembly have the highest completeness compared with the other 15 reported high-quality rice genomes.Further comparison with the japonica rice genome revealed that the gapless indica genome assembly contains more transposable elements(TEs)and segmental duplications(SDs),the latter of which produce many duplicated genes that can affect agronomic traits through dose effect or sub-/neo-functionalization.The insertion of TEs can also affect the expression of duplicated genes,which may drive the evolution of these genes.Furthermore,we found the expansion of nucleotide-binding site with leucine-rich repeat disease-resistance genes and cis-zeatin-O-glucosyltransferase growth-related genes in SDs in the gapless indica genome assembly,suggesting that SDs contribute to the adaptive evolution of rice disease resistance and developmental processes.Collectively,our findings suggest that active TEs and SDs synergistically contribute to rice genome evolution. | Kui Li Wenkai Jiang Yuanyuan Hui Mengjuan Kong Li-Ying Feng Li-Zhi Gao Pengfu Li Shan Lu | 2021 | Molecular Plant2021,14,10: | 6 |
| 4 | Crystallization Behavior and Kinetics of Lithium Aluminosilicate Glasses with Various Li_(2)O Contents显示文摘The crystallization behavior of Li_(2)O-Al_(2)O_(3)-SiO_(2) glasses with various concentration of Li2O were investigated by DSC,XRD,and FE-SEM.The h/l-quartz s.s.is the main crystalline phase for all of the glass with the heat treatment of 720℃/3 h+800℃/1.5 h while the crystallinity and crystal size increase with the increase of Li2O contents.Due to the negative thermal expansion coefficient of h/l-quartz s.s.,the thermal expansion coefficient of glass-ceramics decreases with the increasing of Li2O contents.When the Li2O content is 9mol%,the near-zero CTE20-700℃=-0.09×10^(-6)℃^(-1) is obtained upon the heat treatment of 720℃/3 h+800℃/1.5 h.The crystallization activation energy of the glass with 9mol%Li2O is about 351 kJ/mol,and the crystallization mechanism is volume crystallization. | 韩建军 HONG Wei 姜宏 HE Jianxiong WANG Qianchen WANG Jing GAO Wenkai | 2021 | Journal of Wuhan University of Technology(Materials Science)2021,36,2: | 3 |
| 5 | Study of usingcomplementary S matrix to enhance SNR in Hadamardspeclrometer显示文摘 | Zhang Wenkai Zhang Zhihai Gao Lingxiao | 2014 | Optik2014,125,3: | 1 |
| 6 | A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism显示文摘Calciphylaxis is a rare disease characterized histologically by microvessel calcification and microthrombosis,with high mortality and no proven therapy.Here,we reported a severe uremic calciphylaxis patient with progressive skin ischemia,large areas of painful malodorous ulcers,and mummified legs.Because of the worsening symptoms and signs refractory to conventional therapies,treatment with human amnion-derived mesenchymal stem cells(hAMSCs)was approved.Preclinical release inspections of hAMSCs,efficacy,and safety assessment,including cytokine secretory ability,immunocompetence,tumorigenicity,and genetics analysis in vitro,were introduced.We further performed acute and long-term hAMSC toxicity evaluations in C57BL/6 mice and rats,abnormal immune response tests in C57BL/6 mice,and tumorigenicity tests in neonatal Balbc-nu nude mice.After the preclinical research,the patient was treated with hAMSCs by intravenous and local intramuscular injection and external supernatant application to the ulcers.When followed up to 15 months,the blood-based markers of bone and mineral metabolism improved,with skin soft tissue regeneration and a more favorable profile of peripheral blood mononuclear cells.Skin biopsy after 1-month treatment showed vascular regeneration with mature noncalcified vessels within the dermis,and 20 months later,the re-epithelialization restored the integrity of the damaged site.No infusion or local treatment-related adverse events occurred.Thus,this novel long-term intravenous combined with local treatment with hAMSCs warrants further investigation as a potential regenerative treatment for uremic calciphylaxis due to effects of inhibiting vascular calcification,stimulating angiogenesis and myogenesis,anti-inflammatory and immune modulation,multidifferentiation,re-epithelialization,and restoration of integrity. | Lianju Qin Jing Zhang Yujie Xiao Kang Liu Yugui Cui Fangyan Xu Wenkai Ren Yanggang Yuan Chunyan Jiang Song Ning Xiaoxue Ye Ming Zeng Hanyang Qian Anning Bian Fan Li Guang Yang Shaowen Tang Zhihong Zhang Juncheng Dai Jing Guo Qiang Wang Bin Sun Yifei Ge Chun Ouyang Xueqiang Xu Jing Wang Yaoyu Huang Hongqing Cui Jing Zhou Meilian Wang Zhonglan Su Yan Lu Di Wu Jingping Shi Wei Liu Li Dong Yinbing Pan Baiqiao Zhao Ying Cui Xueyan Gao Zhanhui Gao Xiang Ma Aiqin Chen Jie Wang Meng Cao Qian Cui Li Chen Feng Chen Youjia Yu Qiang Ji Zhiwei Zhang Mufeng Gu Xiaojun Zhuang Xiaolin Lv Hui Wang Yanyan Pan Ling Wang Xianrong Xu Jing Zhao Xiuqin Wang Cuiping Liu Ningxia Liang Changying Xing Jiayin Liu Ningning Wang | 2022 | Journal of Molecular Cell Biology2022,14,2: | 1 |
| 7 | Large-scale controllable fabrication of aluminum nanobowls for surface plasmon-enhanced fluorescence显示文摘Al nanoparticles(NPs)exhibit excellent localized surface plasmon resonance(LSPR)properties and have been considered a promising alternative to plasmonic Au or Ag NPs.However,it remains difficult to fabricate Al NPs with uniform size and controllable morphology over a large area on substrates,which seriously hinders the in-depth exploration of their properties and applications.Herein,we have developed a self-assembly nanoparticle template method to realize the controllable preparation of bowl-shaped Al NPs(Al nanobowls(Al NBs))with tunable sizes from 36 to 131 nm on the substrate surface,accompanied by tunable LSPR spectral responses from 272 to 480 nm.Among them,131 nm Al NBs exhibit superior fluorescence enhancement ability(1932.2-fold)and a low detection limit(78.6 pM)towards 5-carboxyfluorescein,exceeding comparable Ag NBs and Au nanospheres(NSs).This can be attributed to the strong electromagnetic enhancement induced by the LSPR effect and the effective inhibition of fluorescence quenching caused by the self-passivated oxide layer.Therefore,the successful fabrication of Al NBs on substrates is of vital significance for their promising applications,including surface-enhanced spectroscopy,sensitive fluorescence detection,light-harvesting devices,biosensing,and ultraviolet(UV)plasmonics. | Yawen Wang Heng Gao Yuanlan Liu Dong Li Bo Zhao Wenkai Liang Yinghui Sun Lin Jiang | 2023 | Nano Research2023,16,7: | 0 |
| 8 | Neither Fluorocarbons nor Silicones:Hydrocarbon-Based Water-Borne Healable Supramolecular Elastomer with Unprecedent Dual Resistance to Water and Organic Solvents显示文摘Except for fluorocarbons and silicones,few artificial materials display both water and organic solvent resistance.Herein,we report an artificial hydrocarbonbased supramolecular elastomer that displays unprecedented dual resistance to both water and organic solvents.This elastomer is formed in water with two water soluble components,folic acid(FA)and branched poly(ethylene imine)(b-PEI).The hydrogen bonding between the two components occurs immediately,which further promotes the hydrogen bonding between the FA,yielding folate quartets and theirπ-πstacking.These multiple noncovalent interactions work synergistically,leading to a hydrophobic,transparent,stretchable,healable,notchinsensitive,and recyclable elastomer that resists both water and organic solvents.The elastomer remains intact after storage in high humidity and organic solvents for months.Films generated by stretching the elastomer are very hydrophobic,and the contact angle of water on the elastomer is up to 112.3°.The damaged elastomers also exhibit water-assisted healable behavior.This work discloses a new paradigm of material synthesis that can inspire the fabrication of robust nonfluorocarbon and silicone-based materials with green strategies. | Shuitao Gao Shasha Jiang Jinwan Qi Tongyue Wu Wenkai Wang Zhijie Liu Bin Zhang Jianbin Huang Yun Yan | 2022 | CCS Chemistry2022,4,12: | 0 |
| 9 | A Straight Look at Present Situation and Problems with CDM显示文摘An overview of carbon emissions tradingin China and in the world ●With fast development of CDM, the global carbon mar-ket was doubled in 2007 compared with that in 2006.●China’s volume of certified emission reductions (CERs)trading and expected annual emission reductions top the world.Xie Fei:Climate change and energy supply has become one of | Gao Guangsheng: Director, Office of National Coordination Committee on Climate Change Wang Zhixuan: Deputy Secretary, China Electricity Council Xie fei: Department Chief, China Center for Clean Development Mechanism Fund Management Zeng Shaojun: Secretary, New Energy Chamber of Commerce, All-China Federation of Industry & Commerce Zhang Wenkai | 2008 | Electricity2008,19,4: | 0 |
| 10 | Facile fabrication of a single-particle platform with high throughput via substrate surface potential regulated large-spacing nanoparticle assembly显示文摘Nanodevices based on the single nanoparticle represent innovative and promising technology,which could satisfy the increasing requirements of high accuracy,low energy consumption,and small volume.However,the acquisition of single particles involves complex operation,and the corresponding nanodevices display low-throughput.Herein,we present a facile strategy to construct a single-particle platform with high throughput via substrate surface potential modulated a large-area and large-spacing nanoparticle assembly.Such platform not only avoids optic interference but also ensures the independent electrically conductive channel of single particle on substrate.Therefore,the dark-field microscopic imaging and single-particle scattering signals collecting of individual nanoparticles with plasmonic effect are satisfactory achieved based on the platform,and the first success in the fabrication of nano-organic-light-emitting-diodes with single nanoparticle resolution in nanoscale.All the results indicate that the strategy may find promising applications in the in situ single-particle research such as single-particle detection,singleparticle catalysis,and optoelectronics. | Dong Li Yinghui Sun Yawen Wang Yuanlan Liu Bo Zhao Wenkai Liang Heng Gao Lin Jiang | 2022 | Nano Research2022,15,7: | 0 |
| 11 | Detour-phased perovskite ultrathin planar lens using direct femtosecond laser writing显示文摘Perovskite-enabled optical devices have drawn intensive interest and have been considered promising candidates for integrated optoelectronic systems.As one of the important photonic functions,optical phase modulation previously was demonstrated with perovskite substrate and complex refractive index engineering with laser scribing.Here we report on the new scheme of achieving efficient phase modulation by combining detour phase design with 40 nm ultrathin perovskite films composed of nanosized crystalline particles.Phase modulation was realized by binary amplitude patterning,which significantly simplifies the fabrication process.Perovskite nanocrystal films exhibit significantly weak ion migration effects under femtosecond laser writing,resulting in smooth edges along the laser ablated area and high diffractive optical quality.Fabrication of a detour-phased perovskite ultrathin planar lens with a diameter of 150μm using femtosecond laser scribing was experimentally demonstrated.A high-performance 3D focus was observed,and the fabrication showed a high tolerance with different laser writing powers.Furthermore,the high-quality imaging capability of perovskite ultrathin planar lenses with a suppressed background was also demonstrated. | WENKAI YANG LIGE LIU DASHAN DONG XIN ZHANG HAN LIN YUNKUN WANG HONG YANG YUNAN GAO HAIZHENG ZHONG BAOHUA JIA KEBIN SHI | 2022 | Photonics Research2022,10,12: | 0 |
| 12 | Solid-phase molecular self-assembly facilitated supramolecular films with alternative hydrophobic/hydrophilic domains for skin moisture detection显示文摘Human skin moisture is closely related to health and personal care issues.Many creams were developed to claim capable of skin moisturizing.However,people can hardly check their skin moisture status and the moisturizing effect of various creams conveniently,since currently all the skin moisturizing detection rely on large equipment requiring power supply.Herein,we report a power-supply independent supramolecular film that is able to directly report the moisturizing status of the skin.With the strategy of solid-phase molecular self-assembly(SPMSA),the aqueous precipitates formed by commercially available polyelectrolyte and oppositely charged surfactant would transform into a supramolecular film under a mild mechanical pressure,where the hydrophobic surfactant domains bridged by polyelectrolyte merge into hydrophobic mesophases to reduce interfacial energy.The hydrophobic mesophase will greatly retard water diffusion inside the film,so that a humidity gradient is generated as the film is exposed to a humid environment.The film will bend upward automatically in touch with moisture skin,and the bending degree is proportional to the skin moisture status.Therefore,the film can be used as portable smart power-free skin moisture sensor. | Jinwan Qi Tongyue Wu Wenkai Wang Hongjun Jin Shuitao Gao Shasha Jiang Jianbin Huang Yun Yan | 2022 | Aggregate2022,3,2: | 0 |
| 13 | Annotating TSSs in Multiple Cell Types Based on DNA Sequence and RNA-seq Data via DeeReCT-TSS显示文摘The accurate annotation of transcription start sites(TSSs)and their usage are critical for the mechanistic understanding of gene regulation in different biological contexts.To fulfill this,specific high-throughput experimental technologies have been developed to capture TSSs in a genome-wide manner,and various computational tools have also been developed for in silico prediction of TSSs solely based on genomic sequences.Most of these computational tools cast the problem as a binary classification task on a balanced dataset,thus resulting in drastic false positive predictions when applied on the genome scale.Here,we present Dee Re CT-TSS,a deep learningbased method that is capable of identifying TSSs across the whole genome based on both DNA sequence and conventional RNA sequencing data.We show that by effectively incorporating these two sources of information,Dee Re CT-TSS significantly outperforms other solely sequence-based methods on the precise annotation of TSSs used in different cell types.Furthermore,we develop a meta-learning-based extension for simultaneous TSS annotations on 10 cell types,which enables the identification of cell type-specific TSSs.Finally,we demonstrate the high precision of DeeReCT-TSS on two independent datasets by correlating our predicted TSSs with experimentally defined TSS chromatin states.The source code for Dee Re CT-TSS is available at http://gffzzec4b27d8b73d463ascp60nxon9vpk6uf5.ffgz.tsg.suse.edu.cn/Joshua Chou2018/Dee Re CT-TSS_release and http://gffzz77e3413bc06540edscp60nxon9vpk6uf5.ffgz.tsg.suse.edu.cn/biocode/tools/BT007316. | Juexiao Zhou Bin Zhang Haoyang Li Longxi Zhou Zhongxiao Li Yongkang Long Wenkai Han Mengran Wang Huanhuan Cui Jingjing Li Wei Chen Xin Gao | 2022 | Genomics, Proteomics & Bioinformatics2022,20,5: | 0 |
| 14 | Optical engineering of infrared PbS CQD photovoltaic cells for wireless optical power transfer systems显示文摘Infrared photovoltaic cells(IRPCs)have attracted considerable attention for potential applications in wireless optical power transfer(WOPT)systems.As an efcient fber-integrated WOPT system typically uses a 1550 nm laser beam,it is essential to tune the peak conversion efciency of IRPCs to this wavelength.However,IRPCs based on lead sulfde(PbS)colloidal quantum dots(CQDs)with an excitonic peak of 1550 nm exhibit low short circuit current(Jsc)due to insufcient absorption under monochromatic light illumination.Here,we propose comprehensive optical engineering to optimize the device structure of IRPCs based on PbS CQDs,for 1550 nm WOPT systems.The absorption by the device is enhanced by improving the transmittance of tin-doped indium oxide(ITO)in the infrared region and by utilizing the optical resonance efect in the device.Therefore,the optimized device exhibited a high short circuit current density of 37.65 mA/cm^(2)under 1 sun(AM 1.5G)solar illumination and 11.91 mA/cm^(2)under 1550 nm illumination 17.3 mW/cm^(2).Furthermore,the champion device achieved a record high power conversion efciency(PCE)of 7.17%under 1 sun illumination and 10.29%under 1550 nm illumination.The PbS CQDs IRPCs under 1550 nm illumination can even light up a liquid crystal display(LCD),demonstrating application prospects in the future. | Mengqiong Zhu Yuanbo Zhang Shuaicheng Lu Zijun Wang Junbing Zhou Wenkai Ma Ruinan Zhu Guanyuan Chen Jianbing Zhang Liang Gao Jiancan Yu Pingqi Gao Jiang Tang | 2023 | Frontiers of Optoelectronics2023,16,2: | 0 |