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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Sedimentary Characteristics and Model of Gravity Flow Depositional System for the First Member of Upper Miocene Huangliu Formation in Dongfang Area, Yinggehai Basin, Northwestern South China Sea显示文摘The gravity flow deposit were mainly developed in the lowstand systems tract(LST) of the first member of Upper Miocene Huangliu Formation(Ehl1) in Dongfang area, Yinggehai Basin, has become a valuable target for gas exploration and production. The gravity flow sedimentary characteristics of lithofacies associations, sedimentary texture, seismic facies and logging facies were described in detail on the basis of integrated analysis of cores, logging and seismic data. The sedimentary microfacies types composed of neritic sandbar, continental shelf mud, main channel, bifurcated or cross-cutting distributary channel, overspill, and natural levee are revealed under the constraint of high resolution sequence stratigraphic framework in the Ehl1. The gravity flow deposit system in the LST is divided into three evolution stages corresponding to periods of three parasequence sets. The gravity flow deposit was induced in the early LST, expanded rapidly in the middle LST and decreased slightly in the late LST. But its developing scale decreased sharply in the transgression systems tract(TST) and finally vanished in the highstand systems tract(HST). This spatial evolution rule is constrained by the integrated function of sediments supply of the Vietnam Blue River in the LST, the development of local gradient change in sea floor(micro-topography, i.e., flexure slope break), and the fall in relative sea level. On the basics of the deep study of the coupling relationship among the three main control factors, the sedimentary model is established as an optimal component of 'source-channel-sink' for shallow marine turbidite submarine fan. | Ming Sun Hua Wang Jihua Liao Huajun Gan Jun Xiao Jinfeng Ren Shu'e Zhao | 2014 | Journal of Earth Science2014，25，3： | 9 |
| 3 | Toxicity of Graphene Quantum Dots in Zebrafish Embryo显示文摘Objective To evaluate the bio-safety of graphene quantum dots(GQDs), we studied its effects on the embryonic development of zebrafish. Methods In vivo, biodistribution and the developmental toxicity of GQDs were investigated in embryonic zebrafish at exposure concentrations ranging from 12.5-200 μg/m L for 4-96 h post-fertilization(hpf). The mortality, hatch rate, malformation, heart rate, GQDs uptake, spontaneous movement, and larval behavior were examined. Results The fluorescence of GQDs was mainly localized in the intestines and heart. As the exposure concentration increased, the hatch and heart rate decreased, accompanied by an increase in mortality. Exposure to a high level of GQDs(200 μg/mL) resulted in various embryonic malformations including pericardial edema, vitelline cyst, bent spine, and bent tail. The spontaneous movement significantly decreased after exposure to GQDs at concentrations of 50, 100, and 200 μg/mL. The larval behavior testing(visible light test) showed that the total swimming distance and speed decreased dose-dependently. Embryos exposed to 12.5 μg/mL showed hyperactivity while exposure to higher concentrations(25, 50, 100, and 200 μg/mL) caused remarkable hypoactivity in the light-dark test. Conclusion Low concentrations of GQDs were relatively non-toxic. However, GQDs disrupt the progression of embryonic development at concentrations exceeding 50 μg/mL. | WANG Zhen Guo ZHOU Rong JIANG Dan SONG Jing E XU Qian SI Jing CHEN Yun Ping ZHOU Xin GAN Lu LI Jian Zhen ZHANG Hong LIU Bin | 2015 | Biomedical and Environmental Sciences2015，28，5： | 6 |
| 4 | Monitoring gene expression profile changes in ovarian carcinomas using cDNA microarray显示文摘 | Wang K Gan L Jeffery E | | 0，，： | 2 |
| 5 | Comparison of the mor-phine sparing effects of diclofenac sodium and ketorolactromethamine after major orthopedic surgery显示文摘 | Alexander R El-Moalem H E Gan T J | 2002 | J Clin Anesth2002，14，3： | 1 |
| 6 | Reservoir effect and the role of low current density regions on electromi- gration lifetimes in copper interconnects 显示文摘 | Gan Z H Shao W Mhaisalkar S G e! al | 2006 | Journal of Material Research2006，21，9： | 1 |
| 7 | Technical barriers and development of Cu wirebonding in nanoelectronics device packaging显示文摘 | GAN C L NG E K CHAN B L | 2012 | Journal of Nanomaterials2012，1730，： | 1 |
| 8 | Class Ⅲ beta-tubulin mediates sensitivity to chemotherapeutic drugs in non small cell lung cancer显示文摘 | Gan P P Pasquier E Kavallaris M | | 0，，19： | 1 |
| 9 | Silence in group psychotherapy: a powerful communication 显示文摘 | Gans J Counselman E | 2000 | Int J Group Psychother2000，50，1： | 1 |
| 10 | The antimicrobial effects in vivo of minocycline,doxycycline and tetracycline in human显示文摘 | Leyden J J Kaidbey K Gans E H | 1996 | J Dermatol Treat1996，7，： | 1 |
| 11 | Monitoring gene expression profile changes in ovarian carcinomas using CDNA microarray 显示文摘 | Wang K Gan L Jeffery E | 1999 | Gene1999，229，： | 1 |
| 12 | Class III beta-tubulin mediates sensitivity to chemotherapeutic drugs in non small cell lung cancer显示文摘 | Gan PP Pasquier E Kavallaris M | 2007 | Cancer Res2007，67，19： | 1 |
| 13 | Transforming C60 molecules into graphene quantum dots 显示文摘 | Lu J Yeo P S E Gan C K | 2011 | Nature Nan- otechnology2011，6，4： | 1 |
| 14 | Class Ⅲ beta-tubulin mediates sensitivity to chemotherapeutic drugs in non-small cell lung cancer显示文摘 | Gan PP Pasquier E Kavallaris M | | 0，，19： | 1 |
| 15 | The effect of local anesthetic infiltration around nephrostomy tract on postoperative pain control after percutaneous nephrolithotomy显示文摘 | Tüzel E Kiziltepe G Akdoˇgan B | 2009 | Urolithiasis2009，8，4： | 1 |
| 16 | Monitoring gene expression profile changes in ovarian Careinomas using cDNA microarry显示文摘 | wang K Gan L Jeffery E | 1999 | Gene1999，229，： | 1 |
| 17 | Monitoring gene expression profile changes in ovarian carcinomas using cDNA microarray 显示文摘 | Wang K Gan L Jeffery E | 1999 | Gene1999，229，12： | 1 |
| 18 | A hardware in the loop simulation platform for vision-based control of unmanned air vehicles 显示文摘 | Gans N R Dixon W E Lind R Kurdila A | 2009 | Mechatronics2009，19，7： | 1 |
| 19 | Breast Screening and Health Issues among Rural Females in Malaysia:How Much do They Know and Practice?显示文摘 | Dahlui M Gan D E Taib N A | 2013 | Prey Med2013，，57： | 1 |
| 20 | Polymerization of Styrene in a Winsor I--Like System 显示文摘 | LOH S E GAN L M LIAN N | 1994 | Langmuir1994，10，7： | 1 |
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