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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
| 3 | Characteristics of fecal microbiota in non-alcoholic fatty liver disease patients显示文摘This study was designed to investigate the gut microbiota of patients with non-alcoholic fatty liver disease. The inclusive and exclusive criteria for NAFLD patients and healthy subjects were formulated, and detailed clinical data were collected. The genomic DNA of stool samples were extracted for 16 S r DNA sequencing, and the amplified V4-region was sequenced on the Illumina Miseq platform. Metastats analysis was performed to identify the differential taxa between the groups. Redundancy analysis was used to evaluate the association between gut microbial structure and clinical variables. Thirty NAFLD patients and37 healthy controls were involved. The 16 S r DNA sequencing showed that there was a dramatic variability of the fecal microbiota among all the individuals. Metastats analysis identified eight families and 12 genera with significant differences between the two groups. When some clinical parameters, such as waist-to-hip ratio(WHR) and homeostasis model assessment of insulin resistance(HOMA-IR), were enrolled in Redundancy analysis, the distribution of the two group of samples was obviously changed. The compositional shifts in fecal bacterial communities of NAFLD patients from the healthy controls were mainly at family or genus levels. According to our Redundancy analysis, insulin resistance and obesity might be closely related to both NAFLD phenotype and intestinal microecology. | Fan Li Gang Sun Zikai Wang Wenming Wu He Guo Lihua Peng Lili Wu Xu Guo Yunsheng Yang | 2018 | Science China(Life Sciences)2018，61，7： | 9 |
| 4 | Insight into the Ebola virus nucleocapsid assembly mechanism: crystal structure of Ebola virus nucleoprotein core domain at 1.8 A resolution显示文摘 | Shishang Dong Peng Yang Guobang Li Baocheng Liu Wenming Wang Xiang Liu Boran Xia Cheng Yang Zhiyong Lou Yu Guo Zihe Rao | 2015 | Protein & Cell2015，6，5： | 6 |
| 5 | Methane dry reforming on Ni/La_2Zr_2O_7 treated by plasma in different atmospheres显示文摘A series of Ni/La2Zr2O7 pyrochlore catalysts prepared by impregnation method and treated by dielectric barrier discharge(DBD) plasma in different atmospheres and varied sequences were prepared and applied for dry reforming of methane(DRM). It is found that all of the plasma treated catalysts show evidently improved activity and coke resistance in comparison with the non-plasma treated one. The best performance is achieved on Ni/La2Zr2O7–H2P–C,a catalyst treated in H2 plasma before calcination. TGA-DSC and SEM demonstrate that carbon deposition is significantly suppressed on all of the plasma treated samples. Moreover,XRD and TEM results testify that both Ni O and Ni sizes on the calcined and reduced samples treated by plasma are also decreased,which results in higher Ni metal dispersion on the reduced and used catalysts and enhances the interactions between Ni sites and the support. It is believed that these are the inherent reasons accounting for the promotional effects of plasma treatment on the reaction performance of the Ni/La2Zr2O7 pyrochlore catalysts. | Honggen Peng Youhe Ma Wenming Liu Xianglan Xu Xiuzhong Fang Jie Lian Xiang Wang Changqing Li Wufeng Zhou Ping Yuan | 2015 | Journal of Energy Chemistry2015，24，4： | 4 |
| 6 | Targeted energy transfer of a parallel nonlinear energy sink显示文摘A parallel nonlinear energy sink(NES) is proposed and analyzed. The parallel NES is composed of a vibro-impact(VI) NES and a cubic NES. The dynamical equation is given, and the essential analytical investigation is carried out to deal with the cubic nonlinearity and impact nonlinearity. Multiple time-scale expansion is introduced, and the zeroth order is derived to give a rough outline of the system. The underlying Hamilton dynamic equation is given, and then the optimal stiffness is expressed. The clearance is regarded as a critical factor for the VI. Based on the periodical impact treatment by analytical investigation, the relationships of the cubic stiffness, the clearance, and the zeroth-order attenuation amplitude of the linear primary oscillator(LPO) are obtained.A cubic NES under the optimal condition is compared with the parallel NES. Harmonic signals, harmonic signals with noises, and the excitation generated by a second-order?lter are considered as the potential excitation forces on the system. The targeted energy transfer(TET) in the designed parallel NES is shown to be more e?cient. | Yimin WEI Sha WEI Qianlong ZHANG Xinjian DONG Zhike PENG Wenming ZHANG | 2019 | Applied Mathematics and Mechanics(English Edition)2019，40，5： | 4 |
| 7 | Confined Ni-In intermetallic alloy nanocatalyst with excellent coking resistance for methane dry reforming显示文摘Carbon dioxide and methane are two main greenhouse gases which are contributed to serious global warming.Fortunately,dry reforming of methane(DRM),a very important reaction developed decades ago,can convert these two major greenhouse gases into value-added syngas or hydrogen.The main problem retarding its industrialization is the seriously coking formation upon the nickel-based catalysts.Herein,a series of confined indium-nickel(In-Ni)intermetallic alloy nanocatalysts(In_(x)Ni@SiO_(2))have been prepared and displayed superior coking resistance for DRM reaction.The sample containing 0.5 wt.%of In loading(In_(0.5)Ni@SiO_(2))shows the best balance of carbon deposition resistance and DRM reactivity even after 430 h long term stability test.The boosted carbon resistance can be ascribed to the confinement of core–shell structure and to the transfer of electrons from Indium to Nickel in In-Ni intermetallic alloys due to the smaller electronegativity of In.Both the silica shell and the increase of electron cloud density on metallic Ni can weaken the ability of Ni to activate C–H bond and decrease the deep cracking process of methane.The reaction over the confined InNi intermetallic alloy nanocatalyst was conformed to the Langmuir-Hinshelwood(L-H)mechanism revealed by in situ diffuse reflectance infrared Fourier transform spectroscopy(in-situ DRIFTS).This work provides a guidance to design high performance coking resistance catalysts for methane dry reforming to efficiently utilize these two main greenhouse gases. | Wenming Liu Le Li Sixue Lin Yiwei Luo Zhenghong Bao Yiru Mao Kongzhai Li Daishe Wu Honggen Peng | 2022 | Journal of Energy Chemistry2022，31，2： | 3 |
| 8 | Parametric identification of time-varying systems from free vibration using intrinsic chirp component decomposition显示文摘Time-varying systems are applied extensively in practical applications,and their related parameter identification techniques are of great significance for structural health monitoring of time-varying systems.To improve the identification accuracy for time-varying systems,this study puts forward a novel parameter identification approach in the time-frequency domain using intrinsic chirp component decomposition(ICCD).ICCD is a powerful tool for signal decomposition and parameter extraction,with good signal reconstruction capability in a high-noise environment.To maintain good identification effects for the time-varying system in a noisy environment,the proposed method introduces a redundant Fourier model for the non-stationary signal,including instantaneous frequency(IF)and instantaneous amplitude(IA).The accuracy and effectiveness of the proposed approach are demonstrated by a single-degree-of-freedom system with three types of time-varying parameters,as well as an example of a multi-degree-of-freedom system.The effects of different levels of measured noise on the identified results are also discussed in detail.Numerical results show that the proposed method is very effective in tracking the smooth,periodical,and non-smooth variations of time-varying systems over the entire identification time period even when the response signal is contaminated by intense noise. | Sha Wei Shiqian Chen Xingjian Dong Zhike Peng Wenming Zhang | 2020 | Acta Mechanica Sinica2020，36，1： | 3 |
| 9 | Non-stationary signal analysis based on general parameterized time-frequency transform and its application in the feature extraction of a rotary machine显示文摘 | Peng ZHOU Zhike PENG Shiqian CHEN Yang YANG Wenming ZHANG | 2018 | Frontiers of Mechanical Engineering2018，13，2： | 3 |
| 10 | Magnetic levitation using diamagnetism:Mechanism,applications and prospects显示文摘As a typical contact-free manipulation technique that removes friction and contamination risk,levitation has gradually become a preferred candidate for various applications.Magnetic levitation using diamagnetism,beyond Earnshaw’s theorem,is a kind of passive stable levitation that can be achieved at normal temperatures with no energy input.Appealingly,most seemingly nonmagnetic materials can be levitated in a magnetic field and can stabilize free levitation of magnetic materials.This review focuses on the fundamental principles of magnetic levitation using diamagnetism,with emphasis on its burgeoning applications.The theoretical basis associated with the magnetic levitation using diamagnetism is discussed by elucidating the characteristics of diamagnetic materials,and the key levitation mechanisms are clarified.Afterwards,state-of-the-art applications in various aspects,including sensing and measurement,actuating and micromanipulation,energy harvesting and magnetic gravity compensation,are summarized and compared.Finally,the review concludes with a brief outlook on future perspectives. | GAO QiuHua YAN Han ZOU HongXiang LI WenBo PENG ZhiKe MENG Guang ZHANG WenMing | 2021 | Science China(Technological Sciences)2021，64，1： | 3 |
| 11 | Light-induced protein translocation by genetically encoded unnatural amino acid in Caenorhabditis elegans显示文摘Dear Editor,Living systems such as Caenorhabditis elegans are organized by interaction networks of biopolymer and small mol-ecules.The molecular engineering of specifi c targets to monitor and control these biological molecules has led to significant advances in our understand-ing of how biological systems are organ-ized,maintain themselves and disin-tegrate(Prescher and Bertozzi,2005).For instance,green fl uorescent protein(GFP)derived from jellyfi sh Aequorea Victoria helps us to accurately locate and observe proteins over long term periods.In addition,many novel LOV(light,oxy-gen,or voltage)domains such as LOV2-Jαand Vivid offer the possibility of cell-based motility management and tissue-based gene expression control(Wu et al.,2009;Wang et al.,2012). | Hao Chang Mei Han Wenming Huang Guifeng Wei Juanjuan Chen Peng R.Chen Runsheng Chen Junlong Zhang Tao Xu Pingyong Xu | 2013 | Protein & Cell2013，4，12： | 2 |
| 12 | Ln2Zr2O7 compounds(Ln=La,Pr, Sm, Y) with varied rare earth A sites for low temperature oxidative coupling of methane显示文摘With the objective to develop catalysts having application potential for oxidative coupling of methane (OCM) at relatively lower temperature. A series of Ln2Zr2O7 compounds with varied rare earth A sites have been prepared by a co-precipitation method. XRD and Raman have proved that pure Ln2Zr2O7 compounds have been successfully prepared for all the catalysts. By decreasing the rA/rB ratio, their crystalline structure transform from an ordered pyrochlore (La2Zr2O7) to a less ordered pyrochlore (Pr2Zr2O7 and Sm2Zr2O7) and eventually to a defective cubic fluorite phase (Y2Zr2O7). H2-TPR, O2-TPD and XPS have testified that the amount of surface active O2-species follows the order of La2Zr2O7>Pr2Zr2O7>Sm2Zr2O7 > Y2Zr2O7, which is well consistent with the reaction performance, indicating that the abundance of surface O2- sites is a critical factor influencing the reaction performance. CO2-TPD has demonstrated that a better catalyst generally possesses a larger amount of surface moderate alkaline sites, which is another factor to affect the reaction performance. It is concluded that the concerted interaction between the two types of surface active sites controls the reaction performance of the Ln2Zr2O7 catalysts. In comparison with the state-of-the art Mn/Na2WO4/SiO2, La2Zr2O7, the best catalyst, exhibits much improved reaction performance below 750 ℃. | Xiuzhong Fang Lianghui Xia Liang Peng Yuan Luo Junwei Xu Luoji Xu Xianglan Xu Wenming Liu Renyang Zheng Xiang Wang | 2019 | Chinese Chemical Letters2019，30，6： | 2 |
| 13 | Responses of Seed Yield and Economic Benefit of Winter Oilseed Rape(Brassica napus L.) to Different NPK Ratio and Dose显示文摘Field experiments were conducted during 2008-2010 to investigate the effects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape(Brassica napus L.)- Xiangzayou763,and to determine the optimum NPK ratio and dose in Hunan,China.The results showed that NPK ratio(1:0.50:0.50) with 180 kg/hm2 N,90 kg/hm^2 P_2O_5 and 90 kg/hm^2 K_2O was the optimum combination for the highest seed yield(2 231.13 kg/hm2) and economic benefit(9 816.97 Yuan/hm^2),of which 1 641.23 Yuan/hm^2 being set for fertilizer investment(VCR=4.11).Besides,the effects of N,P and K fertilizers on seed yield and oil production of winter oilseed rape were not identical.N had the greatest effect on plant growth of winter oilseed rape,followed by P and K.Total leaf number,green leaf number,leaf length and width,rhizome diameter,branch number,dry weight per plant and other agronomic characters in high N treatments were higher than those in low N treatments.By correlation and path analysis of yield components,the contribution rate to seed yield was found:effective silique number per plant>seed number per silique>1 000-seed weight. | Yingzi WANG Chang TIAN Xuan ZHOU Wenming WANG Jianwei PENG Zhenhua ZHANG Haixing SONG Chunyun GUAN | 2015 | Agricultural Science & Technology2015，16，11： | 2 |
| 14 | Overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) is associated with human cervical cancer malignancy, progression and prognosis显示文摘 | Xue Peng Zhao Wu Lei Yu Jinke Li Wenming Xu Hsiao Chang Chan Yi Zhang Lina Hu | 2012 | Gynecologic Oncology2012，，： | 1 |
| 15 | Modal identification of multi-degree-of-freedom structures based on intrinsic chirp component decomposition method显示文摘Modal parameter identification is a mature technology.However,there are some challenges in its practical applications such as the identification of vibration systems involving closely spaced modes and intensive noise contamination.This paper proposes a new time-frequency method based on intrinsic chirp component decomposition(ICCD)to address these issues.In this method,a redundant Fourier model is used to ameliorate border distortions and improve the accuracy of signal reconstruction.The effectiveness and accuracy of the proposed method are illustrated using three examples:a cantilever beam structure with intensive noise contamination or environmental interference,a four-degree-of-freedom structure with two closely spaced modes,and an impact test on a cantilever rectangular plate.By comparison with the identification method based on the empirical wavelet transform(EWT),it is shown that the presented method is effective,even in a high-noise environment,and the dynamic characteristics of closely spaced modes are accurately determined. | Sha WEI Shiqian CHEN Zhike PENG Xingjian DONG Wenming ZHANG | 2019 | Applied Mathematics and Mechanics(English Edition)2019，40，12： | 1 |
| 16 | Efficient hydrogengas production from cassava and food waste by a two-step processof dark fermentation and photo- fermentation显示文摘 | Zong Wenming Yu Ruisong Zhang Peng | 2009 | Biomass andBioeneiiy2009，33，10： | 1 |
| 17 | Novel fea-ture fusion method for speech emotion recognition basedon multiple kernel learning 显示文摘 | Jin Yun Song Peng Zheng Wenming | 2013 | Journal of Southeast Uni-versity2013，29，2： | 1 |
| 18 | Simultaneous infection with dengue 2 and 3 viruses in a Chinese patient return from Sri Lanka显示文摘 | Peng Wenming Yu man Fan Baochang Deng Yongqiang Jiang Tao Duan Hongyuan Qin Ede | 2004 | Journal of Clinical Virology2004，，： | 1 |
| 19 | Guidelines for the diagnosis and treatment of acute pancreatitis in China (2021)显示文摘Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role in promoting the standardization of AP diagnosis and treatment and improving the treatment quality of AP patients.On the basis of previous guidelines and expert consensus,this guideline adopts an evidence-based,problem-based expression;synthesizes important clinical research data at home and abroad in the most recent 5 years;and forms 29 recommendations through multidisciplinary expert discussion,including diagnosis,treatment,and follow-up.It is expected to provide evidence support for the treatment of AP in the clinical setting in China. | Fei Li Shouwang Cai Feng Cao Rufu Chen Deliang Fu Chunlin Ge Chunyi Hao Jihui Hao Heguang Huang Zhixiang Jian Gang Jin Ang Li Haimin Li Shengping Li Weiqin Li Yixiong Li Tingbo Liang Xubao Liu Wenhui Lou Yi Miao Yiping Mou Chenghong Peng Renyi Qin Chenghao Shao Bei Sun Guang Tan Xiaodong Tian Huaizhi Wang Lei Wang Wei Wang Weilin Wang Junmin Wei Heshui Wu Wenming Wu Zheng Wu Changqing Yan Yinmo Yang Xiaoyu Yin Xianjun Yu Chunhui Yuan Taiping Zhang Yupei Zhao on behalf of the Chinese Pancreatic Surgery Association | 2021 | Journal of Pancreatology2021，4，2： | 1 |
| 20 | Continued antigenic variation of highly pathogenic avian influenza A(H7N9)virus in laying hens in China,2020–2021显示文摘Dear Editor,Since it first appeared in humans in China in 2013,a novel H7N9influenza virus has caused 1,568 cases of infection,with a mortality rate of 39.2%(Yin et al.,2021).The early H7N9 virus strains had low pathogenicity and did not cause avian disease(Zhang et al.,2013).However,they caused concern about poultry consumption and caused huge economic losses to the poultry industry.Since mid-2016,some H7N9 viruses mutated. | Wenming Jiang Xin Yin Shuo Liu Shaobo Liang Cheng Peng Guangyu Hou Jinping Li Xiaohui Yu Yang Li Jingjing Wang Hualei Liu | 2022 | Virologica Sinica2022，37，4： | 1 |
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