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| 1 | Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum var.dicoccoides) is tightly linked in repulsion with stripe rust resistance gene Yr5显示文摘Stripe rust and powdery mildew are both devastating diseases for durum and common wheat.Pyramiding of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum var.dicoccoides) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust. | Deyun Zhang Keyu Zhu Lingli Dong Yong Liang Genqiao Li Tilin Fang Guanghao Guo Qiuhong Wu Jingzhong Xie Yongxing Chen Ping Lu Miaomiao Li Huaizhi Zhang Zhenzhong Wang Yan Zhang Qixin Sun Zhiyong Liu | 2019 | The Crop Journal2019，7，6： | 8 |
| 2 | Identification of powdery mildew resistance loci in wheat by integrating genome-wide association study(GWAS) and linkage mapping显示文摘Wheat powdery mildew(Blumeria graminis f.sp.tritici, Bgt) is a disease of increasing importance globally due to the adoption of high yielding varieties and modern sustainable farming technologies.Growing resistant cultivars is a preferred approach to managing this disease, and novel powdery mildew resistance genes are urgently needed for new cultivar development.A genome-wide association study was performed on a panel of 1292 wheat landraces and historical cultivars using 5011 single nucleotide polymorphism(SNP)markers.The association panel was evaluated for reactions to three Bgt inoculants, OKS(14)-B-3-1, OKS(14)-C-2-1, and Bgt15.Linkage disequilibrum(LD) analysis indicated that genome-wide LD decayed to 0.1 at 23 Mb, and population structure analysis revealed seven subgroups in the panel.Association analysis using a mixed linear model(MLM) identified three loci for powdery mildew resistance on chromosome 2 B, designated QPm.stars-2BL1,QPm.stars-2BL2, and QPm.stars-2BL3.To evaluate the efficacy of GWAS in gene discovery,QPm.stars-2BL2 was validated using F2 and F2:3 populations derived from PI420646 × OK1059060-126135-3.Linkage analysis delimited the powdery mildew resistance gene in PI 420646 to an interval where QPm.stars-2BL2 was located, lending credence to the GWAS results.QPm.stars-2BL1 and QPm.stars-2BL3, which were associated with four SNPs located at 457.7–461.7 Mb and two SNPs located at 696.6–715.9 Mb in the Chinese Spring reference IWGSC RefSeq v1.0, respectively, are likely novel loci for powdery mildew resistance and can be used in wheat breeding to improve powdery mildew resistance. | Genqiao Li Xiangyang Xu Chengcheng Tan Brett F.Carver Guihua Bai Xuewen Wang J.Michael Bonman Yanqi Wu Robert Hunger Christina Cowger | 2019 | The Crop Journal2019，7，3： | 2 |
| 3 | Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26显示文摘 | Li Genqiao Li Zaifeng Yang Wuyun | 2006 | Theoretical and Applied Genetics2006，112，8： | 1 |
| 4 | Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)显示文摘 | Genqiao Li Tilin Fang Hongtao Zhang Chaojie Xie Hongjie Li Tsomin Yang Eviatar Nevo Tzion Fahima Qixin Sun Zhiyong Liu | 2009 | Theoretical and Applied Genetics2009，，： | 1 |
| 5 | Characterization of Rsg2.a3:A new greenbug resistance allele at the Rsg2 locus from wild barley(Hordeum vulgare ssp.spontaneum)显示文摘Greenbug(Schizaphis graminum Rondani)is a destructive insect pest that not only damages plants,but also serves as a vector for many viruses.Host plant resistance is the preferred strategy for managing greenbug.Two greenbug resistance genes,Rsg1 and Rsg2,have been reported in barley.To breed cultivars with effective resistance against various greenbug biotypes,additional resistance genes are urgently needed to sustain barley production.Wild barley accession WBDC053(PI 681777)was previously found to be resistant to several greenbug biotypes.In this study,a recombinant inbred line(RIL)population derived from Weskan×WBDC053 was evaluated for response to two greenbug biotypes(E and TX1)and genotyped using genotyping by sequencing(GBS).A set of 3347 high quality GBS-derived single nucleotide polymorphisms(SNPs)were then used to map the greenbug resistance gene in this wild barley accession.Linkage analysis placed the greenbug resistance gene in a 2.35 Mb interval(0-2,354,645 bp)in the terminal region of the short arm of chromosome 2H.This interval harbors 15 genes with leucine-rich-repeat(LRR)protein domains.An allelism test indicated that the greenbug resistance gene in WBDC053,designated Rsg2.a3,is likely allelic or closely linked to Rsg2.GBS-SNPs 2H_1318811and 2H_1839499 co-segregating with Rsg2.a3 in the RIL population were converted to Kompetitive allele specific PCR(KASP)markers KASP-Rsg2.a3-1 and KASP-Rsg2.a3-2,respectively.The two KASP markers can be used to select Rsg2.a3 and have the potential to tag Rsg2 in barley improvement programs. | Xiangyang Xu Dolores Mornhinweg Amy Bernardo Genqiao Li Ruolin Bian Brian J.Steffenson Guihua Bai | 2022 | The Crop Journal2022，10，6： | 0 |
| 6 | Genomic location of Gb1, a unique gene conferring wheat resistance to greenbug biotype F显示文摘Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it essential to explore all greenbug resistant sources available to manage this pest. Gb1, a recessive greenbug resistance gene in DS28A, confers resistance to several economically important greenbug biotypes and is the only gene found to be resistant to greenbug biotype F. A set of 174 F_(2:3)lines from the cross DS28A × Custer was evaluated for resistance to greenbug biotype F in 2020 and 2022. Selective genotyping of the corresponding F_(2) population using single nucleotide polymorphism(SNP) markers generated by genotyping-by-sequencing(GBS) led to the identification of a candidate genomic region for Gb1. Thus, SSR markers previously mapped in this region were used to genotype the entire F2population,and kompetitive allele specific PCR(KASP) markers were also developed from SNPs in the target region.Gb1 was placed in the terminal region of the short arm of chromosome 1A, and its location was confirmed in a second population derived from the cross DS28A × PI 697274. The combined data analysis from the two mapping populations delimited Gb1 to a < 1 Mb interval between 13,328,200 and 14,241,426 bp on1AS. | Xiangyang Xu Genqiao Li Guihua Bai Brett FCarver Ruolin Bian Amy Bernardo JScott Armstrong | 2023 | The Crop Journal2023，11，5： | 0 |
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