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| 1 | Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem L.)显示文摘WRKY proteins are involved in various physiological processes,including biotic and abiotic stress responses,hormone responses and development.However,no systematic identification,expression and function analysis of WRKY genes in wheat were reported.In this study,we isolated 15 wheat cDNAs with complete open reading frame(ORF)encoding putative WRKY proteins using in silico cloning.Phylogenetic analysis indicated that the 15 wheat WRKY genes belonged to three major WRKY groups.Expression analysis revealed that most genes expressed drastically in leaf,except TaWRKY10 which expressed in crown intensively.Four genes were strongly up-reg-ulated with the senescence of leaves.Eight genes were responsive to low temperature,high temperature,NaCl or PEG treatment.More-over,dierential expression patterns were also observed between wheat hybrid and its parents,and some genes were more responsive to PEG treatment in the hybrid.These results demonstrated that wheat WRKY genes are involved in leaf senescing and abiotic stresses.And the changed expression of these WRKY genes in hybrid might contribute to the heterosis by improving the stress tolerance in hybrids. | Hualing Wu Zhongfu Ni Yingyin Yao Ganggang Guo Qixin Sun | 2008 | Progress in Natural Science:Materials International2008，18，6： | 34 |
| 2 | Load Profiling and Its Application to Demand Response: A Review显示文摘The smart grid has been revolutionizing electrical generation and consumption through a two-way flow of power and information. As an important information source from the demand side, Advanced Metering Infrastructure(AMI) has gained increasing popularity all over the world. By making full use of the data gathered by AMI, stakeholders of the electrical industry can have a better understanding of electrical consumption behavior. This is a significant strategy to improve operation efficiency and enhance power grid reliability. To implement this strategy,researchers have explored many data mining techniques for load profiling. This paper performs a state-of-the-art,comprehensive review of these data mining techniques from the perspectives of different technical approaches including direct clustering, indirect clustering, clustering evaluation criteria, and customer segmentation. On this basis, the prospects for implementing load profiling to demand response applications, price-based and incentivebased, are further summarized. Finally, challenges and opportunities of load profiling techniques in future power industry, especially in a demand response world, are discussed. | Yi Wang Qixin Chen Chongqing Kang Mingming Zhang Ke Wang Yun Zhao | 2015 | Tsinghua Science and Technology2015，20，2： | 17 |
| 3 | A Collinearity-Incorporating Homology Inference Strategy for Connecting Emerging Assemblies in the Triticeae Tribe as a Pilot Practice in the Plant Pangenomic Era显示文摘Plant genome sequencing has dramatically increased,and some species even have multiple high-quality reference versions.Demands for clade-specific homology inference and analysis have increased in the pangenomic era.Here we present a novel method,GeneTribe(http://gffzz6c7fcf032cb94ff5skknxbwubkov96pwx.ffgz.tsg.suse.edu.cn/genetribe/),for homology inference among genetically similar genomes that incorporates gene collinearity and shows bet-ter performance than traditional sequence-similarity-based methods in terms of accuracy and scalability.The Triticeae tribe is a typical allopolyploid-rich clade with complex species relationships that includes many important crops,such as wheat,barley,and rye.We built Triticeae-GeneTribe(http://gffzzaeec8df70e7c4b21hkknxbwubkov96pwx.ffgz.tsg.suse.edu.cn/TGT/),a homology database,by integrating 12 Triticeae genomes and 3 outgroup model genomes and implemented versatile analysis and visualization functions.With macrocollinearity analysis,we were able to construct a refined model illustrating the structural rearrangements of the 4A-5A-7B chromosomes in wheat as two major translocation events.With collinearity analysis at both the macro-and microscale,we illustrated the complex evolutionary history of homologs of the wheat vernalization gene Vm2,which evolved as a combined result of genome translocation,duplication,and polyploidization and gene loss events.Our work provides a useful practice for connecting emerging genome assemblies,with awareness of the extensive polyploidy in plants,and will help researchers efficiently exploit genome sequence re-sources. | Yongming Chen Wanjun Song Xiaoming Xie Zihao Wang Panfeng Guan Huiru Peng Yuannian Jiao Zhongfu Ni Qixin Sun Weilong Guo | 2020 | Molecular Plant2020，13，12： | 14 |
| 4 | Analysis of miR-205 and miR-155 expression in the blood of breast cancer patients显示文摘The purpose of this study was to identify and validate circulating microRNAs (miRNAs) in human plasma for use as breast cancer (BC) biomarkers and to analyze their relationship to clinicopathologic features and its preliminary biological function. Genome-wide expression profiling of miRNAs in BC was investigated by microarray analysis. miR-155 was up-regulated greater than two-fold in BC compared with Normal Adjacent Tissue (NAT), whereas let-7b, miR-381, miR-10b, miR-125a-5p, miR-335, miR-205 and miR-145 were down- regulated greater than two-fold. Our hypothesis was that circulating miRNAs are also present and differentially expressed in the serum of BC patients compared to controls. Using real-time PCR (RT-PCR), we analyzed miR-205 and miR-155 in archived serum from 30 participants, 20 with breast cancer and 10 healthy people. miR-205 was down-regulated in BC patient serum while miR-155 was up-regulated. Furthermore, we analyzed the relationship between the expression levels of these two miRNAs and the clinicopathologic parameters of BC patients. High expression of miR155 was associated with clinical stage, molecular type, Ki-67 and p53 in BC patients (P<0.05). By contrast, we found no significant correlation between miR-205 and BC patient clinicopathologic parameters. Functional analysis showed that ectopic expression of miR-205 significantly inhibits cell proliferation and promotes apoptosis. miR-205 was down-regulated and miR-155 was up-regulated in BC patient serum. miR-155 was positive correlated with clinical stage and ki-67 and negatively correlated with p53 status. | Jingjing Liu Qixin Mao Yan Liu Xiaomeng Hao Sheng Zhang Jin Zhang | 2013 | Chinese Journal of Cancer Research2013，25，1： | 14 |
| 5 | Terrestrial water variations in the North China Plain revealed by the GRACE mission显示文摘Using eight years of time-variant gravity measurements from the GRACE gravity satellite mission, we estimate monthly terrestrial water storage variations in the North China Plain between August 2002 and August 2010. We find that during this period, the water storage is not constant but accelerate at a rate of -1.1 cm/yr over time. The study confirms that the relatively long-term trend of water storage in the North China Plain (by 13-point moving average) is consistent well with that by linear fitting. Two hydrological models, CPC and GLDAS, are adopted in calculating surface water variations, with results indicating that they agree with those of GRACE. Furthermore, rates of -0.6 cm/yr for surface water variations and -0.5 cm/yr for groundwater variations are found in the North China Plain during the study period. Decrease of rainfall and the groundwater over-exploitation are possibly the main causes for groundwater depletion in the North China Plain. The results will be helpful for better understanding climatic changes and provide reference for the management of water resources and the establishment of policies on preventing and alleviating natural hazards. | SU XiaoLi PING JinSong YE QiXin | 2011 | Science China Earth Sciences2011，54，12： | 13 |
| 6 | Allelopathic effects of gallic acid from Aegiceras corniculatum on Cyclotella caspia显示文摘High abundance of algae and eutrophication were observed in mangrove wetlands and these were estimated to be associated with root exudates of some specific mangrove plants to a certain extent.Root exudates form allelopathic effects from mangroves.The main secondary metabolites of Aegiceras corniculatum had been detected to be organic phenolic acids.Gallic acid had been isolated and identified from A.corniculatum.The half-maximal inhibitory concentration of gallic acid on alge Cyclotella caspia was tested as 15.46mg/L.The effects on algal cell morphology were mainly shown as elongated cells,with no apparent cell inclusions,such as oil droplets,chloroplast.At a dose of 2mg/L,gallic acid had a stimulative effect on the specific growth rate of algae on day 3.The contents of malondialdehyde,superoxide dismutase,soluble carbohydrates and chlorophyll a in algal cells showed an overall 'low promotion and high suppression'.Our results could provide preliminary and valuable reference on the complex influences of mangroves on microecology and microbial communities in the rhizosphere system. | Yu Liu Fei Li Qixin Huang | 2013 | Journal of Environmental Sciences2013，25，4： | 11 |
| 7 | Force Control Compensation Method with Variable Load Stiffness and Damping of the Hydraulic Drive Unit Force Control System显示文摘Each joint of hydraulic drive quadruped robot is driven by the hydraulic drive unit(HDU), and the contacting between the robot foot end and the ground is complex and variable, which increases the difficulty of force control inevitably. In the recent years, although many scholars researched some control methods such as disturbance rejection control, parameter self-adaptive control, impedance control and so on, to improve the force control performance of HDU, the robustness of the force control still needs improving. Therefore, how to simulate the complex and variable load characteristics of the environment structure and how to ensure HDU having excellent force control performance with the complex and variable load characteristics are key issues to be solved in this paper. The force control system mathematic model of HDU is established by the mechanism modeling method, and the theoretical models of a novel force control compensation method and a load characteristics simulation method under different environment structures are derived, considering the dynamic characteristics of the load stiffness and the load damping under different environment structures. Then, simulation effects of the variable load stiffness and load damping under the step and sinusoidal load force are analyzed experimentally on the HDU force control performance test platform, which provides the foundation for the force control compensation experiment research. In addition, the optimized PID control parameters are designed to make the HDU have better force control performance with suitable load stiffness and load damping, under which the force control compensation method is introduced, and the robustness of the force control system with several constant load characteristics and the variable load characteristics respectively are comparatively analyzed by experiment. The research results indicate that if the load characteristics are known, the force control compensation method presented in this paper has positive compensation effects on the load characteristics variation, i.e., this method decreases the effects of the load characteristics variation on the force control performance and enhances the force control system robustness with the constant PID parameters, thereby, the online PID parameters tuning control method which is complex needs not be adopted. All the above research provides theoretical and experimental foundation for the force control method of the quadruped robot joints with high robustness. | KONG Xiangdong BA Kaixian YU Bin CAO Yuan ZHU Qixin ZHAO Hualong | 2016 | Chinese Journal of Mechanical Engineering2016，29，3： | 10 |
| 8 | Effect of Selenium on Root Oxidizing Ability and Yield of Rice under Ferrous Stress显示文摘To study the effects of selenium on root oxidizing ability and yield of rice under ferrous stress, a pot culture experiment was conducted, the activity of glutathione peroxidase (GSH-Px) and the concentration of malonaldelyde (MDA) were determined. The root oxidizing ability and yield characters of rice were examined. Results showed that appropriate amount of Se enhanced the activity of glutathione peroxidase and the oxidizing ability of rice roots significantly, reduced the concentration of MDA, increased 1000-grain weight of rice, F = 26.96**, decreased empty and blighted grain rate, increased the rice yield, F = 11.53**, and enhanced the rice resistance under ferrous stress. | QIXin LIUYuan-ying SONGTian-xing | 2004 | Journal of Northeast Agricultural University(English Edition)2004，11，1： | 9 |
| 9 | A bioactive injectable self-healing anti-inflammatory hydrogel with ultralong extracellular vesicles release synergistically enhances motor functional recovery of spinal cord injury显示文摘The repair and motor functional recovery after spinal cord injury(SCI)remains a worldwide challenge.The inflammatory microenvironment is one of main obstacles on inhibiting the recovery of SCI.Using mesenchymal stem cells(MSCs)derived extracellular vesicles to replace MSCs transplantation and mimic cell paracrine secretions provides a potential strategy for microenvironment regulation.However,the effective preservation and controlled release of extracellular vesicles in the injured spinal cord tissue are still not satisfied.Herein,we fabricated an injectable adhesive anti-inflammatory F127-polycitrate-polyethyleneimine hydrogel(FE)with sustainable and long term extracellular vesicle release(FE@EVs)for improving motor functional recovery after SCI.The orthotopic injection of FE@EVs hydrogel could encapsulate extracellular vesicles on the injured spinal cord,thereby synergistically induce efficient integrated regulation through suppressing fibrotic scar formation,reducing inflammatory reaction,promoting remyelination and axonal regeneration.This study showed that combining extracellular vesicles into bioactive multifunctional hydrogel should have great potential in achieving satisfactory locomotor recovery of central nervous system diseases. | Chenggui Wang Min Wang Kaishun Xia Jingkai Wang Feng Cheng Kesi Shi Liwei Ying Chao Yu Haibin Xu Shining Xiao Chengzhen Liang Fangcai Li Bo Lei Qixin Chen | 2021 | Bioactive Materials2021，6，8： | 9 |
| 10 | Molecular mapping of QTLs for root response to phosphorus deficiency at seedling stage in wheat (Triticum aestivum L.)显示文摘Phosphorus (P) deficiency in the soil is one of the major abiotic stresses that limit plant growth and crop productivity throughout the world. Development of cultivars with improved P-deficiency tolerance is an efficient strategy for sustainable agriculture. Plant roots play an important role in crop growth and development, especially in nutrient uptake and improvement of P-efficiency. Mapping quantitative trait loci (QTLs) for root traits and their response to low P stress at seedling stage will facilitate the development of P-efficient wheat cultivars. In this study, 30 QTLs (LOD>2.0) were mapped for the three root traits, such as root length, root number and root dry matter under different P supply conditions and their response to P-stress. These QTLs were distributed on 14 chromosomes, with each of the 5 QTLs explaining more than 10% phenotype variance. Analyses showed that root traits and their response to P-deficiency were controlled by different QTLs. In addition, alleles with positive effects were separated on both parents, and wheat cultivars with improved P-efficiency could be developed by accumulating these positive effect alleles together. | Li Zhenxing Ni Zhongfu Peng Huiru Liu Zhiyong Nie Xiuling Xu Shengbao Liu Gang Sun Qixin | 2007 | Progress in Natural Science:Materials International2007，17，10： | 8 |
| 11 | Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum var.dicoccoides) is tightly linked in repulsion with stripe rust resistance gene Yr5显示文摘Stripe rust and powdery mildew are both devastating diseases for durum and common wheat.Pyramiding of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum var.dicoccoides) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust. | Deyun Zhang Keyu Zhu Lingli Dong Yong Liang Genqiao Li Tilin Fang Guanghao Guo Qiuhong Wu Jingzhong Xie Yongxing Chen Ping Lu Miaomiao Li Huaizhi Zhang Zhenzhong Wang Yan Zhang Qixin Sun Zhiyong Liu | 2019 | The Crop Journal2019，7，6： | 8 |
| 12 | A natural variation in Ribonuclease H-like gene underlies Rht8 to confer“Green Revolution”trait in wheat显示文摘Dear Editor,Introduction of gibberellin(GA)-insensitive Reduced height(Rht)genes,Rht-B1b and Rht-D1b,has resulted in the“Green Revolution”in modern wheat cultivars(Triticum aestivum)that has skyrocketed wheat grain yields worldwide since the 1960s(Peng et al.,1999;Velde et al.,2021).However,Rht-B1b/D1b also reduce coleoptiles,which is undesired in dryland regions where deep planting is essential for seedling establishment(Rebetzke et al.,1999,Rebetzke et al.,2001;Ellis et al.,2004). | Lingling Chai Mingming Xin Chaoqun Dong Zhaoyan Chen Huijie Zhai Junhong Zhuang Xuejiao Cheng Naijiao Wang Jia Geng Xiaobo Wang Ruolin Bian Yingyin Yao Weilong Guo Zhaorong Hu Huiru Peng Guihua Bai Qixin Sun Zhenqi Su Jie Liu Zhongfu Ni | 2022 | Molecular Plant2022，15，3： | 8 |
| 13 | Optimal operation strategy for distributed battery aggregator providing energy and ancillary services显示文摘The high penetration of distributed renewable energy raises a higher concern for the safe and economic operation of the smart grid. Distributed batteries equipped in demand-side can not only contribute to the reliability and security of the grid, but also make profits by participating in the electricity market, especially when distributed batteries are combined and operated by an aggregator.Considering the well-operated mechanism of performance based regulation(PBR) in the U.S. electricity market, it becomes increasingly lucrative for batteries to participate not only in energy markets for energy arbitrage, but also in ancillary service markets to provide regulation and peakload shaving services. In this study, distributed batteries are operated and coordinated by the aggregator, which simultaneously submits offers to the energy and the ancillary service markets as an individual entity. An optimaldecision model is formulated for the aggregator to determine the operation and bidding strategy for the distributed batteries by considering the characteristics of batteries,including the terms of capacity, efficiency and degradation cost. Finally, a numerical case is conducted to evaluate the benefits of the decision model. | Kangning LIU Qixin CHEN Chongqing KANG Wei SU Guobin ZHONG | 2018 | Journal of Modern Power Systems and Clean Energy2018，6，4： | 7 |
| 14 | Molecular dissection of plant height QTLs using recombinant inbred lines from hybrids between common wheat (Triticum aestivum L.) and spelt wheat (Triticum spelta L.)显示文摘In wheat, plant height is an important agronomic trait, and a number of quantitative trait loci (QTLs) controlling plant height have been located. In this study, using the conditional and unconditional QTL mapping methods, combined with data from five different growth stages over two years of field trials, the developmental behavior for plant height in wheat was dissected. Nine unconditional QTLs and 8 conditional QTLs were identified, of which 6 were detected by both methods. None of the 11 QTLs was detected at all of the 5 investigated developmental stages, but 7 QTLs were detected at certain stages in both years. Further analysis identified 9 unconditional QTLs at different stages, which could explain the phenotypic variation from 4.81% to 17.35%. It was noteworthy that one major QTL designated QHt-4B-2, which was located on chromosome 4B, was detected on May 18 and 25 in both years, and its genetic contributions to plant height ranged from 13.42% to 16.13%. Moreover, of the 8 conditional QTLs identified, six were detected in both years, in the order of QHt-3B→QHt-4B-1→QHt-4B-2→QHt-4D→QHt-5A and QHt-2B expressed at the same developmental stage. The results indicate that QTL expression during plant height development is selective and in a temporal order. | LIU Gang XU ShengBao NI ZhongFu XIE ChaoJie QIN DanDan LI Jing LU LaHu ZHANG JinPing PENG HuiRu SUN QiXin | 2011 | Chinese Science Bulletin2011，56，18： | 6 |
| 15 | TOOL PATH PLANNING USING VORONOI DIAGRAM AND THREE STACKS显示文摘Based on the object oriented data structure of Voronoi diagram, the algorithm of the trimmed offset generating and the optimal too l path planning of the pocket machining for multiply connected polygonal domains are studied. The intersection state transition rule is improved in this algorithm. The intersection is between the trimmed offsets and Voronoi polygon. On this basis, the trimmed offset generating and the optimal tool path planning are mad e with three stacks(I stack, C stack and P stack)in different monotonous pouches of Voronoi diagram. At the same time, a merging method of Voronoi diagram an d offsets generating for multiply connected polygonal domains is also presented. The above algorithms have been implemented in NC machining successfully, and the efficiency is fully verified. | Fu Zhuang,Liu Chengliang,Yin Yuehong,Cao Qixin,Ma Peixun (Research Institute of Robotics, Shanghai Jiaotong University) Wang Shuguo (Harbin Institute of Technology) | 2001 | Chinese Journal of Mechanical Engineering2001，14，4： | 5 |
| 16 | Shaping polyploid wheat for success:Origins,domestication,and the genetic improvement of agronomic traits显示文摘Bread wheat(Triticum aestivum L.,AABBDD,2 n=6 x=42),which accounts for most of the cultivated wheat crop worldwide,is a typical allohexaploid with a genome derived from three diploid wild ancestors.Bread wheat arose and evolved via two sequential allopolyploidization events and was further polished through multiple steps of domestication.Today,cultivated allohexaploid bread wheat has numerous advantageous traits,including adaptive plasticity,favorable yield traits,and extended end-use quality,which have enabled its cultivation well beyond the ranges of its tetraploid and diploid progenitors to become a global staple food crop.In the past decade,rapid advances in wheat genomic research have considerably accelerated our understanding of the bases for the shaping of complex agronomic traits in this polyploid crop.Here,we summarize recent advances in characterizing major genetic factors underlying the origin,evolution,and improvement of polyploid wheats.We end with a brief discussion of the future prospects for the design of gene cloning strategies and modern wheat breeding. | Jie Liu Yingyin Yao Mingming Xin Huiru Peng Zhongfu Ni Qixin Sun | 2022 | Journal of Integrative Plant Biology2022，64，2： | 5 |
| 17 | Genetic improvement of heat tolerance in wheat:Recent progress in understanding the underlying molecular mechanisms显示文摘As a cool season crop, wheat(Triticum aestivum L.) has an optimal daytime growing temperature of 15 ℃ during the reproductive stage. With global climate change, heat stress is becoming an increasingly severe constraint on wheat production. In this review, we summarize recent progress in understanding the molecular mechanisms of heat tolerance in wheat. We firstly describe the impact of heat tolerance on morphology and physiology and its potential effect on agronomic traits. We then review recent discoveries in determining the genetic and molecular factors affecting heat tolerance, including the effects of phytohormone signaling and epigenetic regulation. Finally, we discuss integrative strategies to improve heat tolerance by utilization of existing germplasm including modern cultivars, landraces and related species. | Zhongfu Ni Hongjian Li Yue Zhao Huiru Peng Zhaorong Hu Mingming Xin Qixin Sun | 2018 | The Crop Journal2018，6，1： | 4 |
| 18 | CAPTURE EFFECT OF ELECTRORHEOLOGICAL SUSPENSIONS IN FLOW FIELD显示文摘According to the results of experiments and theoretical analysis,a phenomenon called “capture effect” is put forward,which could be used to describe the particles dynamic behavior of electrorheological (ER) suspensions. Then a “structure-force” mathematical model is established to explain this effect based on electrostatic energy density equation. The analysis results show that the dynamic coupling process of ER suspensions under an external electric filed is the function not only of the electric intensity,but also of the dielectric properties and the structure form. | ZHU Shisha SUN Hongli HUANG Yijian WANG Qixin | 2007 | Chinese Journal of Mechanical Engineering2007，20，2： | 4 |
| 19 | Chemical weathering and CO_2 consumption of a high-erosion-rate karstic river: a case study of the Sanchahe River, southwest China显示文摘The Sanchahe River in southwest China is a tributary of the Wujiang River and experiences high erosion rates. Geochemical analysis was conducted on Sanchahe River basin samples collected in the wet and dry seasons of 2014 in order to better understand local chemical weathering processes, anthropogenic influences, and associated CO2 consumption. The samples' total dissolved solid concentrations were found to be significantly higher than that of the global river average. Ca2+was the dominant cation in the samples and accounted for 64 % and73 % of the total cations in the dry and wet seasons,respectively. HCO3-and SO42-were the dominant anions,accounting for 92 % of the total anions. Stoichiometry analyses of the river waters suggested that the water chemistry is controlled by carbonate dissolution by both carbonic and sulfuric acid. The chemical weathering rates of carbonate and silicate evaporites in the Sanchahe River basin were estimated to be approximately 109.2 and 11.0 t/(km2a), respectively, much higher than both the global mean values and the Wujiang River, a typical karstic river.The CO2 consumption by carbonate and silicate weathering are estimated to be 597.4 9 103 and 325.5 9 103mol/(km2a), which are much higher than corresponding values in the Wujiang River, indicating a high erosion rate in the Sanchahe River basin. | Yanling An Yiliang Hou Qixin Wu Lin Qing Longbo Li | 2015 | Chinese Journal Of Geochemistry2015，34，4： | 4 |
| 20 | Genomic and genic sequence variation in synthetic hexaploid wheat (AABBDD) as compared to their parental species显示文摘In order to understand the genomic changes during the evolution of hexaploid wheat, two sets of synthetic hexaploid wheat from hybridization between maternal tetraploid wheat (AABB) and paternal diploid goat grass (DD) were used for DNA-AFLP and single strand conformation polymorphism (SSCP) analysis to determine the genomic and genic variation in the synthetic hexaploid wheat. Results indicated that more DNA sequences from paternal diploid species were eliminated in the synthetic hexaploid wheat than from maternal tetraploid wheat, suggesting that genome from parental species of lower ploidity tends to be eliminated preferentially. However, sequence variation detected by SSCP procedure was much lower than those detected by DNA-AFLP, which indicated that much less variation in the genic regions occurred in the synthetic hexaploid wheat, and sequence variations detected by DNA-AFLP could be derived mostly from non-coding regions and repetitive sequences. Our results also indicated that sequence variation in 4 genes can be detected in hybrid F1, which suggested that this type of sequence variation could be resulted from distant hybridization. It was interesting to note that 3 out of the 4 genes were mapped and clustered on the long arm of chromosome 2D, which indicated that variation in genic sequences in synthetic hexaploid wheat might not be a randomized process. | Lihong Nie Zongfu Han Lahu Lu Yingyin Yao Qixin Sun Zhongfu Ni | 2008 | Progress in Natural Science:Materials International2008，18，5： | 4 |
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