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| 1 | Detection and characterization of murine colitis and carcinogenesis by molecularly targeted contrast-enhanced ultrasound显示文摘AIM To study mucosal addressin cellular adhesion molecule-1(MAd CAM-1) and vascular endothelial growth factor(VEGF)-targeted contrast enhanced ultrasound(CEUS) for the assessment of murine colitis and carcinogenesis. METHODS C57BL/6 mice were challenged with 3% dextran sodium-sulfate(DSS) for three, six or nine days to study the development of acute colitis. Ultrasound was performed with and without the addition of unspecific contrast agents. MAd CAM-1-targeted contrast agent was used to detect and quantify MAd CAM-1 expression. Inflammatory driven colorectal azoxymethane(AOM)/DSS-induced carcinogenesis was examined on day 42 and 84 using VEGF-targeted contrast agent. Highly specific tissue echogenicity was quantified using specialized software. Sonographic findings were correlated to tissue staining, western blot analysis and immunohistochemistry to quantify the degree of inflammation and stage of carcinogenesis. RESULTS Native ultrasound detected increased general bowel wall thickening that correlated with more progressed and more severe DSS-colitis(healthy mice: 0.3 mm ± 0.03 vs six days DSS: 0.5 mm ± 0.2 vs nine days DSS: 0.6 mm ± 0.2, P < 0.05). Moreover, these sonographic findings correlated well with clinical parameters such as weight loss(r2 = 0.74) and histological damage(r2 = 0.86)(P < 0.01). In acute DSS-induced murine colitis, CEUS targeted against MAd CAM-1 detected and differentiated stages of mild, moderate and severe colitis via calculation of mean pixel contrast intensity in decibel(9.6 d B ± 1.6 vs 12.9 d B ± 1.4 vs 18 d B ± 3.33, P < 0.05). Employing the AOM/DSSinduced carcinogenesis model, tumor development was monitored by CEUS targeted against VEGF and detected a significantly increased echogenicity in tumors as compared to adjacent healthy mucosa(healthy mucosa, 1.6 d B ± 1.4 vs 42 d, 18.2 d B ± 3.3 vs 84 d, 18.6 d B ± 4.9, P < 0.01). Tissue echogenicity strongly correlated with histological analysis and immunohistochemistry findings(VEGF-positive cells in 10 high power fields of healthy mucosa: 1 ± 1.2 vs 42 d after DSS start: 2.4 ± 1.6 vs 84 d after DSS start: 3.5 ± 1.3, P < 0.01). CONCLUSION Molecularly targeted CEUS is a highly specific and noninvasive imaging modality, which characterizes murine intestinal inflammation and carcinogenesis in vivo. | Markus Brückner Jan Heidemann Tobias M Nowacki Friederike Cordes Jorg Stypmann Philipp Lenz Faekah Gohar Andreas Lügering Dominik Bettenworth | 2017 | World Journal of Gastroenterology2017，23，16： | 4 |
| 2 | Hedgehog signaling is required for adult blood stem cell formation in zebrafish embryos显示文摘 | Gering M Patient R | 2005 | Dev Cell2005，，8： | 1 |
| 3 | The effect of the PPARgamma ligand rosiglitazone on energy balance regulation显示文摘 | JOOSEN A M BAKKER A H GERING M J | 2006 | Diabetes Metab Res Rev2006，22，3： | 1 |
| 4 | Faces of suburbaniza- tion:residential location choice at the urban fringe显示文摘 | Cunningham C Espey M Gering L R | 2007 | Review of A- gricultural Economics2007，29，3： | 1 |
| 5 | Treatment for distal tibial fractures with intramedullary nails and blocking screws显示文摘 | Seyhan M Kocaoglu B Gereli A | | 0，，： | 1 |
| 6 | Arterial hypertension 2007: guidelines for themanagement of arterial hypertension 显示文摘 | Gere V Buksa M | 2007 | Med Arh2007，61，21： | 1 |
| 7 | Equilibrium and kinet ics for the sorption of promethazine hydrochloride onto K10 montmo- rillonite显示文摘 | GERELI G SEKI Y KUSOGLU I M | 2006 | Journal of Colloid and Interface Science2006，299，1： | 1 |
| 8 | The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebra fish显示文摘 | Patterson LJ Gering M Eckfeldt CE | 2007 | Blood2007，109，6： | 1 |
| 9 | Treatment for distal tibial fractures with intramedullary nails and blocking screws 显示文摘 | Seyhan M Kocaoglu B Gereli A | 2012 | Europ J Orthop Surg Traumatol2012，22，5： | 1 |
| 10 | The SCL gene specifies haemangioblast development from early mesoderm 显示文摘 | Gering M Rodaway AR Gottgens B | 1998 | Embo J1998，17，17： | 1 |
| 11 | A new enzyme superfamily-the phosphopantetheinyl transferases显示文摘 | Lambalot R H Gering A M Flugel R S | 1996 | Chem Biol1996，3，： | 1 |
| 12 | Analysis of shear cores using superelements显示文摘 | GOODNO B J GERE J M | 1976 | Proceeding ASCE1976，102，1： | 1 |
| 13 | The SCL gene specifies development from early mesoderm 显示文摘 | Gering M Rodaway AR Goettgens B | 1998 | EMBO J1998，17，： | 1 |
| 14 | Serial intraoperative magnetic resonance imaging of brain shift显示文摘 | NABAVI A BLACK P M GERING D T | 2001 | Neurosurgery2001，48，4： | 1 |
| 15 | Notch signalling and haematopoietic stem cell formation during embryogenesis 显示文摘 | Gering M Patient R | 2010 | J Cell Physiol2010，222，1： | 1 |
| 16 | Notch signalling and haematopoietic stem cell formation during em bryogenesis显示文摘 | Gering M Patient R | 2010 | J Cell Physiol2010，222，1： | 1 |
| 17 | Hedgehog signaling is required for adult blood stem cell formation in zebrafish embryos显示文摘 | Gering M Patient R | 2005 | Dev Cell2005，8，3： | 1 |
| 18 | Nonlinear vector measurements and associat- ed behavioral models 显示文摘 | Gering J M | 2011 | IEEE Microwave Magazine2011，，： | 1 |
| 19 | Analysis of shear cores using su- perelements显示文摘 | Goodno B J Gere J M | 1976 | Journal of the Structural Division1976，102，1： | 1 |
| 20 | Earthquake behavior of suspended floor buildings 显示文摘 | Goodno B J Gere J M | 1976 | Journal of the Structural Division1976，102，5： | 1 |
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