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| 1 | Application of poly-lactide-co-glycolide-microspheres in the transarterial chemoembolization in an animal model of hepatocellular carcinoma显示文摘AIM:To introduce an animal model of hepatocellular carcinoma (HCC) in ACI-rats, and to evaluate the therapeutic effects of Poly-lactide-co-glycolide(PIcg)-microspheres in the transarterial chemoembolization (TACE) in this model, as well the value of this model in the experiments of interventional therapy. | Jochen Truebenbach Florian Graepler Philippe Pereira Peter Huppert Thomas Eul Gundula Wiemann Claus Claussen | 2003 | World Journal of Gastroenterology2003，9，1： | 24 |
| 2 | ACI大鼠肝细胞癌模型在介入治疗实验中的初步应用显示文摘目的 评价在ACI大鼠肝细胞癌模型进行多种介入治疗方案的疗效及该模型在介入治疗实验中的应用价值。方法 在 5 8只雄性ACI大鼠肝包膜下植入MorrisHepatoma 3 92 4A肝癌瘤块( 1mm3) ,移植术后 13d行MR检查 ,测量肿瘤体积 (V1) ,第 14天时 ,经腹部切开术及胃十二指肠动脉逆行插管而采取以下介入治疗方案 :(A)丝裂霉素 4只 ;(B)降解淀粉 4只 ;(C)碘油 5只 ;(D)肝动脉结扎 4只 ;(E)丝裂霉素 +降解淀粉 4只 ;(F)丝裂霉素 +动脉结扎 5只 ;(G)丝裂霉素 +碘油 5只 ;(H)降解淀粉 +动脉结扎 4只 ;(I)碘油 +动脉结扎 4只 ;(J)丝裂霉素 +聚丙交酯复合乙交酯 (poly lactid co glycolid ,Plcg)微球 4只 ;(K)丝裂霉素 +碘油 +动脉结扎 4只 ;(L)丝裂霉素 +降解淀粉 +动脉结扎4只 ;(M) 0 9%生理盐水 (对照组 ,7只 )。 13d后再次行磁共振检查以确定肿瘤体积 (V2 )变化 ,并对各组间V2 /V1的比值进行比较。结果 肝癌移植率为 10 0 %。介入治疗后肿瘤体积与治疗前肿瘤体积之比 (V2 /V1)分别为A组 4 5 0 ,B组 12 73 ,C组 15 84,D组 10 17,E组 90 2 0 ,F组 7 16,G组 4 0 8,H组 3 45 ,I组 9 99,J组 2 86,K组 3 76,L组 7 71,M组 2 7 12。与对照组相比 ,采取A、G、H、J和K组方法能明显抑制肿瘤生长 ( χ2 值分别为 5 | 钱骏 Truebenbach Jochen Eul Thomas Graepler Florian Pereira Philippe Huppert Peter 冯敢生 Claussen Claus | 2003 | 中华放射学杂志2003，37，1： | 5 |
| 3 | 聚丙交酯复合乙交酯微球经肝动脉化疗栓塞术治疗肝癌的动物实验显示文摘目的 :在肝细胞癌动物模型上观察聚丙交酯复合乙交酯 (PLcG)微球经肝动脉化疗栓塞术 (TACE)治疗肝癌的疗效。方法 :在雄性ACI大鼠 ( 15例 )肝包膜下植入MorrisHepatoma 392 4A肝癌小瘤块 ( 1mm3) ,移植术后 13天时行磁共振检查。再经正中腹切开术和经胃十二指肠动脉逆行插管进行以下介入治疗 :治疗组A( 40mgPLcG + 0 .0 5mg丝裂霉素 ,4例 )、对照组B( 0 .0 5mg丝裂霉素 + 0 .0 4mg碘化油 +肝动脉结扎 ,4例 )和对照组C( 1.5ml生理盐水 ,7例 )。插管术后 13天再次行磁共振术观察肝肿瘤体积变化。结果 :在C组 ,肿瘤体积在实验期间增长 2 7.12倍 ,在B组 ,肿瘤体积增长 3.76倍 ,而在A组 ,肿瘤体积仅增长 2 .87倍。A组与C组肿瘤体积增长率在t检验时均有显著性差异 (P <0 .0 5 )。结论 :在动物实验中将PLcG微球运用于TACE可明显抑制肝肿瘤生长。 | 钱骏 冯敢生 TruebenbachJ Pereira PL Huppert PE Graepler F Eul T Claussen CD | 2001 | 放射学实践2001，16，6： | 4 |
| 4 | Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model显示文摘AIM: To investigate the effects of catalytically superior gene-directed enzyme prodrug therapy systems on a rat hepatoma model.METHODS: To increase hepatoma cell chemosensitivity for the prodrug 5-fluorocytosine (5-FC), we generated a chimeric bifunctional SuperCD suicide gene, a fusion of the yeast cytosine deaminase (YCD) and the yeast uracil phosphoribosyltransferase (YUPRT) gene.RESULTS: In vitro stably transduced Morris rat hepatoma cells (MH) expressing the bifunctional SuperCD suicide gene (MH SuperCD) showed a clearly marked enhancement in cell killing when incubated with 5-FC as compared with MH ceils stably expressing YCD solely (MH YCD) or the cytosine deaminase gene of bacterial origin(MH BCD), respectively. In vivo, MH SuperCD tumors implanted both subcutaneously as well as orthotopically into the livers of syngeneic ACI rats demonstrated significant tumor regressions (P<0.01) under both high dose as well as low dose systemic 5-FC application,whereas MH tumors without transgene expression (MH naive) showed rapid progression. For the first time, an order of in vivo suicide gene effectiveness (SuperCD>>YCD > > BCD > > > negative control) was defi ned as a result of a directin vivo comparison of all three suicide genes.CONCLUSION: Bifunctional SuperCD suicide gene expression is highly effective in a rat hepatoma model,thereby significantly improving both the therapeutic index and the efficacy of hepatocellular carcinoma killing by fluorocytosine. | Florian Graepler Marie-Luise Lemken Wolfgang A Wybranietz Ulrike Schmidt Irina Smirnow Christine D GroB Martin Spiegel Andrea Schenk Schenk Hansj(o|¨)rg Graf Ulrike A Lauer Reinhard Vonthein Michael Gregor Sorin Armeanu Michael Bitzer Ulrich M.Lauer | 2005 | World Journal of Gastroenterology2005，11，44： | 2 |
| 5 | Combined endostatin/sFlt-1 antiangiogenic gene therapy is highly effective in a rat model of HCC显示文摘 | Graepler F Verbeek B Graeter T | 2005 | Hepatology2005，41，4： | 1 |
| 6 | Endothelial endostatin release is induced by general cell stress and modulated by the nitric oxide/cGMP pathway显示文摘 | Deininger MH Wybranietz WA Graepler FT Lauer UM Meyermann R Schluesener HJ | 2003 | FASEB J2003，17，10： | 1 |
| 7 | Endol;he- lial endostafin release is induced by general cell stress and modulated by the nitric oxide/cGMP pathway显示文摘 | Deininger MH Wybranietz WA Graepler FT | 2003 | FASEB J2003，17，10： | 1 |
| 8 | Growth characteristics and imaging properties of the morris hepatoma 3924A in ACI rats:A suitable model for transarterial chemoembolization显示文摘 | TRUBENBACH J GRAEPLER F PEREIRA P L | 2000 | Cardiovasc Intervent Radiol2000，23，3： | 1 |
| 9 | Endothelial endostatin release is induced by general eell stress and modulated by the nitric oxide/eGMP pathway 显示文摘 | Deininger MH Wybranietz WA Graepler FT | 2003 | FASEB J2003，17，10： | 1 |
| 10 | Endothelial endostatin release is induced by general cell stress and modulated by the nitric oxide/cGMP pathway显示文摘 | Deininger MH Wybranietz WA Graepler FT | 2003 | Faseb J2003，17，10： | 1 |
| 11 | Gombined endostatin/sFl--1 antianglogenic gene therapy is highly effecdve in a rat model of HGG显示文摘 | Graepler F Verbeek B Graeter T | 2005 | Hepatology2005，41，4： | 1 |
| 12 | Magneaic cell sorting for parietal cell purification using a new monoclonal antibody without influence on cell function 显示文摘 | GRAEPLER F LAUER U GREGOR M | 1998 | J Biochem Biophys Moth1998，36，23： | 1 |
| 13 | Combined endo- statin/sFlt-1 antiangiogenic gene therapy is highly effec- tive in a rat model of HCC显示文摘 | Graepler F Verbeek B Graeter T | 2005 | Hepatology2005，41，4： | 1 |
| 14 | Growth characteristics and imaging properties of the morris hepatoma 3924a in ACI rats: A suitable model for transarterial chemoembolization显示文摘 | Jochen Trübenbach Florian Graepler Philippe L Pereira Peter Ruck Ulrich Lauer Michael Gregor Claus-D. Claussen Peter E. Huppert | 2000 | Cardiovascular and Interventional Radiology2000，，3： | 1 |
| 15 | Endothelial endostatin release is induced by general cell stress and modulated by the nitric oxide/cGMP pathway显示文摘 | Deininger MH Wybranietz WA Graepler FTC | 2003 | FASEB J2003，17，10： | 1 |
| 16 | Widespread emergence of methicillin resistance in community-acquired Staphylococcus aureus infections in Denver 显示文摘 | Clancy M J Graepler A Breese P E | 2005 | South Med J2005，98，11： | 1 |
| 17 | Cholangioscopic removal of a dislocated stent from the cystic duct using a high pressure biliary dilation ballon显示文摘 | Graepler F Raible A Neuhaus H | 2007 | Endoscopy2007，39，： | 1 |
| 18 | Fusion of HSV-1 VP22 to a bifunctional chimeric SuperCD suicide gene compensates for low suicide gene transduction efficiencies显示文摘 | Lemken ML Graepler F Wolf C | 2007 | Int J Oncol2007，30，5： | 1 |
| 19 | Growth characteristics and imaging properties of the morris hepatoma 3924A in ACI Rats:A suitable model for transarterial chemoembolization显示文摘 | Jochen Trubenbach Florian Graepler | 2000 | Cardiovaac Intervent Radiol2000，23，3： | 1 |
| 20 | Expression liver-directed genes by employing synthetic transcriptional control units显示文摘AIM: To generate and characterize the synthetic transcriptional control units for transcriptional targeting of the liver,thereby compensating for the lack of specificity of currently available gene therapeutic vector systems.METHODS: Synthetic transcriptional control unit constructs were generated and analyzed for transcriptional activities in different cell types by FACS quantification, semi-quantitative RT-PCR, and Western blotting. RESULTS: A new bifunctionally-enhanced green fluorescent protein (EGFP)/neor fusion gene cassette was generated,and could flexibly be used both for transcript quantification and for selection of stable cell clones. Then, numerous synthetic transcriptional control units consisting of a minimal promoter linked to 'naturally' derived composite enhancer elements from liver-specific expressed genes or binding sites of liver-specific transcription factors were inserted upstream of this reporter cassette. Following liposome-mediated transfection, EGFP reporter protein quantification by FACS analysis identified constructs encoding multimerized composite elements of the apolipoprotein B100 (ApoB) promoter or the ornithin transcarbamoylase (OTC) enhancer to exhibit maximum transcriptional activities in liver originating cell lines, but only background levels in non-liver originating cell lines. In contrast, constructs encoding only singular binding sites of liver-specific transcription factors, namely hepatocyte nuclear factor (HNF)1, HNF3, HNF4, HNF5, or CAAT/enhancer binding protein (C/EBP) only achieved background levels of EGFP expression. Finally, both semi-quantitative RT-PCR and Western blotting analysis of Hep3B cells demonstrated maximum transcriptional activities for a multimeric 4xApoB cassette construct, which fully complied with the data obtained by initial FACS analysis.CONCLUSION: Synthetic transcriptional control unit constructs not only exhibit a superb degree of structural compactness, but also provide new means for liver-directed expression of therapeutic genes. | Marie-Luise Lemken Wolfgang A.Wybranietz Ulrike Schmidt Florian Graepler Sorin Armeanu Michael Bitzer Ulrich M.Lauer | 2005 | World Journal of Gastroenterology2005，11，34： | 0 |
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