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| 1 | FAT1, a direct transcriptional target of E2F1, suppresses cell proliferation, migration and invasion in esophageal squamous cell carcinoma显示文摘Objective: Growing evidence indicates that FAT atypical cadherin 1(FAT1) has aberrant genetic alterations and exhibits potential tumor suppressive function in esophageal squamous cell carcinoma(ESCC). However, the role of FAT1 in ESCC tumorigenesis remains not well elucidated. The aim of this study was to further investigate genetic alterations and biological functions of FAT1, as well as to explore its transcriptional regulation and downstream targets in ESCC.Methods: The mutations of FAT1 in ESCC were achieved by analyzing a combined study from seven published genomic data, while the copy number variants of FAT1 were obtained from an analysis of our previous data as well as of The Cancer Genome Atlas(TCGA) and Cancer Cell Line Encyclopedia(CCLE) databases using the cBioPortal. The transcriptional regulation of FAT1 expression was investigated by chromatin immunoprecipitation(ChIP) and the luciferase reporter assays. In-cell western, Western blot and reverse transcription-quantitative polymerase chain reaction(RT-qPCR) were used to assess the indicated gene expression. In addition, colony formation and Transwell migration/invasion assays were employed to test cell proliferation, migration and invasion.Finally, RNA sequencing was used to study the transcriptomes.Results: FAT1 was frequently mutated in ESCC and was deleted in multiple cancers. Furthermore, the transcription factor E2 F1 occupied the promoter region of FAT1, and depletion of E2 F1 led to a decrease in transcription activity and mRNA levels of FAT1. Moreover, we found that knockdown of FAT1 promoted KYSE30 and KYSE150 cell proliferation, migration and invasion;while overexpression of FAT1 inhibited KYSE30 and KYSE410 cell proliferation, migration and invasion. In addition, knockdown of FAT1 led to enrichment of the mitogen-activated protein kinase(MAPK) signaling pathway and cell adhesion process.Conclusions: Our data provided evidence for the tumor suppressive function of FAT1 in ESCC cells and elucidated the transcriptional regulation of FAT1 by E2 F1, which may facilitate the understanding of molecular mechanisms of the progression of ESCC. | Yu Wang Guangchao Wang Yunping Ma Jinglei Teng Yan Wang Yongping Cui Yan Dong Shujuan Shao Qimin Zhan Xuefeng Liu | 2019 | Chinese Journal of Cancer Research2019，31，4： | 6 |
| 2 | Deregulated expression and subcellular localization of CPSF6,a circRNA-binding protein,promote malignant development of esophageal squamous cell carcinoma显示文摘Objective:Cleavage and polyadenylation specific factor 6(CPSF6)has been documented as an oncoprotein in different types of cancer.However,functions of CPSF6 have not been investigated yet in esophageal squamous cell carcinoma(ESCC).Here,we aimed to investigate the potential clinical values and biological functions of CPSF6 in ESCC.Methods:For determining the expression level of CPSF6 in ESCC patients,we analyzed published data,performed quantitative real-time polymerase chain reaction(RT-qPCR)and immunohistochemistry assays.Kaplan-Meier curves and log-rank tests were used for survival analyses.GO and KEGG analyses were done for CPSF6-related genes.Cell proliferation,colony formation and xenograft assays were conducted to verify the effects of CPSF6 on ESCC.In addition,cell cycle and apoptosis assays were also performed to manifest the functions of CPSF6 and circCPSF6.RNA pulldown and radioimmunoprecipitation(RIP)assays were used for confirming the interaction between circCPSF6(hsa_circ_0000417)and CPSF6 protein.The regulatory relationship between CPSF6 protein and circCPSF6 was determined by RT-qPCR.Results:We found that CPSF6 was upregulated in ESCC tissues and overexpression of cytoplasmic CPSF6 was associated with poor prognosis.GO and KEGG analyses suggested that CPSF6 could mainly affect cell division in ESCC.Further experiments manifested that CPSF6 promoted cell proliferation and colony formation in vitro.Xenograft assay showed that knockdown of CPSF6 significantly decreased tumor growth rate in vivo.Subsequently,we verified that depletion of CPSF6 led to cell cycle arrest and apoptosis.Finally,we validated that CPSF6,as a circRNA-binding protein,interacted with and regulated its circular isoform circCPSF6(hsa_circ_0000417),of which depletion also resulted in cell cycle arrest and cell apoptosis in ESCC.Conclusions:These findings gave us insight that overexpression of cytoplasmic CPSF6 protein is associated with poor prognosis in ESCC and CPSF6 may function as an oncoprotein,at least in part,through regulating circCPSF6 expression. | Shichao Guo Guangchao Wang Zitong Zhao Dan Li Yongmei Song Qimin Zhan | 2022 | Chinese Journal of Cancer Research2022，34，1： | 1 |
| 3 | ZNF292 suppresses proliferation of ESCC cells through ZNF292/SKP2/P27 signaling axis显示文摘Objective:Increasing evidence has demonstrated that ZNF292 plays a suppressive role in cancer,however,little is known about its function and exact mechanism in esophageal squamous cell carcinoma(ESCC).Methods:Bioinformatic analysis and immunohistochemistry(IHC)were performed to analyze the role of ZNF292 in affecting the prognosis of ESCC.Cell proliferation and colony formation ability assays were performed to analyze cell growth after inferring the expression of ZNF292.Flow cytometry was used to analyze changes in the cell cycle upon the depletion of ZNF292.Quantitative real-time polymerase chain reaction(q RT-PCR)and western blot analysis were used to determine the alteration of cell cycle related RNAs and proteins after knocking down ZNF292.MG-132,cycloheximide(CHX)treatment experiments were performed to analyze the change and half-life time of P27 after knockdown of ZNF292.Chromatin immunoprecipitation(Ch IP)and luciferase reporter assays were used to analyze the transcriptional regulation of SKP2 by ZNF292.Results:We report that low expression of ZNF292 is associated with poor prognosis,and ZNF292 emerges to be highly expressed in adjacent and normal tissues rather than tumor tissues in ESCC.Knockdown of ZNF292 significantly boosts cell growth and S phase entry in ESCC cells.ZNF292 depletion will decrease the expression and half-life time of P27,while knockdown of SKP2 will result in elevated expression of P27.ZNF292 can bind to the promoter region of SKP2,and knockdown of ZNF292 will boost the expression of SKP2.Conclusions:Knockdown of ZNF292 mediates G1/S cell cycle procession by activating SKP2/P27 signaling in ESCC cells.ZNF292 knockdown promotes SKP2 expression at the transcriptional level,thereby boosting P27 ubiquitin-degradation,and eventually facilitating the S phase entrance. | Wei Gong Jiancheng Xu Guangchao Wang Dan Li Qimin Zhan | 2021 | Chinese Journal of Cancer Research2021，33，6： | 1 |
| 4 | Biomechanical properties of ready-to-harvest rapeseed plants:Measurement and analysis显示文摘A large loss occurs in the combine harvesting of rapeseeds due to the fragility of rapeseed pods,and all the more so with the vibration of the combine header and the collision between the header and plants.Seed loss is greatly affected by the biomechanical properties of ready-to-harvest rapeseed plants.To understand the mechanism of pod cracking and seed loss and to propose measures for alleviating them,it is needed to study the biomechanical properties of ready-to-harvest rapeseed plants.To this end,“Huayouza 62”,a widely planted rapeseed variety in central China,was selected to study the biomechanical properties,including pod-cracking resistance,main stem-shearing resistance and resonant frequencies,of whole plants.The results showed that the distribution of pod-cracking resistance forces was 1.333–6.100 N in the mature stage,and the pod width and thickness had a significant influence on the cracking resistance.The main influencing factor of the main stem-shearing resistance was the stem diameter.A thicker main stem resulted in a larger shearing resistance force but a smaller shear stress.The moisture contents of the main stems varied from 47.71%to 76.13%.However,the varying moisture contents did not show a significant impact on the shearing resistance.The resonant frequencies of whole rapeseed plants ready for harvest ranged from 6.5 Hz to 7.5 Hz,which was close to the excitation frequency of the cutter bar on the 4LL-1.5Y harvester.This study lays a foundation for improving the design and construction of harvesting devices for rapeseed plants to reduce seed loss. | Guangchao Zhan Wangyuan Zong Lina Ma Junyi Wei Wei Liu | 2023 | Information Processing in Agriculture2023，10，3： | 0 |
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