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| 1 | FAT1, a direct transcriptional target of E2F1, suppresses cell proliferation, migration and invasion in esophageal squamous cell carcinoma显示文摘Objective: Growing evidence indicates that FAT atypical cadherin 1(FAT1) has aberrant genetic alterations and exhibits potential tumor suppressive function in esophageal squamous cell carcinoma(ESCC). However, the role of FAT1 in ESCC tumorigenesis remains not well elucidated. The aim of this study was to further investigate genetic alterations and biological functions of FAT1, as well as to explore its transcriptional regulation and downstream targets in ESCC.Methods: The mutations of FAT1 in ESCC were achieved by analyzing a combined study from seven published genomic data, while the copy number variants of FAT1 were obtained from an analysis of our previous data as well as of The Cancer Genome Atlas(TCGA) and Cancer Cell Line Encyclopedia(CCLE) databases using the cBioPortal. The transcriptional regulation of FAT1 expression was investigated by chromatin immunoprecipitation(ChIP) and the luciferase reporter assays. In-cell western, Western blot and reverse transcription-quantitative polymerase chain reaction(RT-qPCR) were used to assess the indicated gene expression. In addition, colony formation and Transwell migration/invasion assays were employed to test cell proliferation, migration and invasion.Finally, RNA sequencing was used to study the transcriptomes.Results: FAT1 was frequently mutated in ESCC and was deleted in multiple cancers. Furthermore, the transcription factor E2 F1 occupied the promoter region of FAT1, and depletion of E2 F1 led to a decrease in transcription activity and mRNA levels of FAT1. Moreover, we found that knockdown of FAT1 promoted KYSE30 and KYSE150 cell proliferation, migration and invasion;while overexpression of FAT1 inhibited KYSE30 and KYSE410 cell proliferation, migration and invasion. In addition, knockdown of FAT1 led to enrichment of the mitogen-activated protein kinase(MAPK) signaling pathway and cell adhesion process.Conclusions: Our data provided evidence for the tumor suppressive function of FAT1 in ESCC cells and elucidated the transcriptional regulation of FAT1 by E2 F1, which may facilitate the understanding of molecular mechanisms of the progression of ESCC. | Yu Wang Guangchao Wang Yunping Ma Jinglei Teng Yan Wang Yongping Cui Yan Dong Shujuan Shao Qimin Zhan Xuefeng Liu | 2019 | Chinese Journal of Cancer Research2019，31，4： | 6 |
| 2 | Animal diversities and characteristics of environmental change revealed by skeletons unearthed at Zhongba Site of Chongqing City,China显示文摘Nearly 200000 animal skeletons are unearthed in the Unit T0202 from Zhongba Site of Zhongxian County.According to the analysis of 129165 specimens,these skeletons may be classified into 5 kinds, namely,Mammalia,Osteichthys,Aves,Amphibian and Reptilia,which belong to 13 orders,28 families and 42 genera.In this paper,based on archeological dating and AMS 14 C data,through statistically analyzing the unearthed skeletons and studying the change of the smallest individual numbers,these research results detected the following:1)In almost all the time of 2370―200 BC,in Zhongba region, some animals distributed widely,such as Muntiacus sp,Elaphodus cephalophus and Muntiacus sp. inhabiting in glade and grassland,Scrofa sp.and Canis familiaris raised by the ancient people,rodentia rabbit and Rattus rattus,which suggested that a fairly good ecological environment of forest and grassland was preserved at that period and the predecessors began to raise domestic animals from 1750 to 1000 BC,which has lasted until today.2)Rhinoceros only lived during 2000―1750 BC,1000― 700 BC and 700―500 BC,which indicates that the ecological environment of grassland and wetland might be better in these phases.3)Macaca sp.and Ursus arctos appeared only after 1750―1000 BC, which may show that the forest condition is better for animals to live during 1750―200 BC.4)The smallest individual numbers of Bublus sp.,bos sp.and otters emerged during about 2370―1750 BC, which perhaps infers that water area during the early period was wider than that of the late period.5) Since skeletons of rhinoceros are discovered in strata of Zhongba Site during 2000―1750 BC and 1000―500 BC,according to the climate and ecology environment which rhinoceros live in now,the average annual temperature and precipitation during 2000―1750 BC and 1000―500 BC are supposed to be probably higher than that of today.Although Zhu Kezhen considered that the first low temperature period in the past 5000 years of China was between 1100 BC and 850 BC,massive pollen of Morus, Ulmus,Fagus,Quercus,Castanea,etc.were found in Dajiuhu peat at that time,which should indicate that the climate was still moderate for living things to live at least in Dajiuhu basin and Zhongba Site.6) Because skeletons of Gervus albirostris were unearthed during 310―200 BC at Zhongba Site,based onthe climate and the ecological environment those animals live in now,the average annual temperature and the average annual precipitation in Zhongba area between 310 BC and 200 BC should be lower than those of today,which is confirmed by the TOC research of Zhongba Site strata. | ZHU Cheng MA ChunMei OUYANG Jie LI ZhongXuan YIN Qian SUN ZhiBin HUANG YunPing R.K.FLAD LI Lan LI YuMei | 2008 | Chinese Science Bulletin2008，53，S1： | 4 |
| 3 | Proteomic identification of new diagnostic biomarkers of early-stage cutaneousmycosis fungoides显示文摘Dear editor,Mycosis fungoides(MF),the most common subtype of cutaneous T cell lymphoma,is a rare disease[1,2].Patients with early-stageMF have a 5-year overall survival between 88%and 100%[3].In patients with advanced MF,the skin may present with tumors and erythroderma,and the median survival of patients with lymph node and visceral metastasis is 13 months[4].Therefore,it is important to achieve an early diagnosis to improve prognosis[5]. | Ling Leng Zhaorui Liu Jie Ma Shiyu Zhang Yukun Wang Luye Lv Yunping Zhu Dunqin Gao Yujie Wang Juncheng Wang Yuehua Liu Jie Liu | 2022 | Cancer Communications2022，42，6： | 4 |
| 4 | Pathological features of COVID-19-associated lung injury:a preliminary proteomics report based on clinical samples显示文摘The COVID-19 pandemic has emerged as a global health emergency due to its association with severe pneumonia and relative high mortality.However,the molecular characteristics and pathological features underlying COVID-19 pneumonia remain largely unknown.To characterize molecular mechanisms underlying COVID-19 pathogenesis in the lung tissue using a proteomic approach,fresh lung tissues were obtained from newly deceased patients with COVID-19 pneumonia.After virus inactivation,a quantitative proteomic approach combined with bioinformatics analysis was used to detect proteomic changes in the SARS-CoV-2-infected lung tissues.We identified significant differentially expressed proteins involved in a variety of fundamental biological processes including cellular metabolism,blood coagulation,immune response,angiogenesis,and cell microenvironment regulation.Several inflammatory factors were upregulated,which was possibly caused by the activation of NF-κB signaling.Extensive dysregulation of the lung proteome in response to SARS-CoV-2 infection was discovered.Our results systematically outlined the molecular pathological features in terms of the lung response to SARS-CoV-2 infection,and provided the scientific basis for the therapeutic target that is urgently needed to control the COVID-19 pandemic. | Ling Leng Ruiyuan Cao Jie Ma Danlei Mou Yunping Zhu Wei Li Luye Lv Dunqin Gao Shikun Zhang Feng Gong Lei Zhao Bintao Qiu Haiping Xiang Zhongjie Hu Yingmei Feng Yan Dai Jiang Zhao Zhihong Wu Hongjun Li Wu Zhong | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 4 |
| 5 | Comparison of surgical indications for hysterectomy by age and approach in 4653 Chinese women显示文摘约 100 万子宫切除每年在中国被执行。然而,关于指示的国家数据和为子宫切除的外科的途径正在缺乏。这研究的目的是在中国女人为子宫切除在不同年龄组和不同外科的途径的相对优点为子宫切除检验外科的指示。从从 2004 ~ 2009 在 Tongji 医院里执行的子宫切除的 4653 个盒子的临床的数据被分析。子宫切除最通常 4049 年在女人之中被执行(2299;49.4%) 。总的来说, colporrhagia 和腹的疼痛是为子宫切除的二个很普通的指示。由年龄组的最普通的指示如下:恶意的卵巢的瘤, 70 年。恶意的原因论的比例也到年龄变化了,在女人是最高的变老 P < 0.05 ) 。两个都,流血和输血体积的数量要求了在阴道途径是更小的,没有其它之间的重要差别。使用的外科的途径也与外科的范围有关。外科的指示和双边的 oophorectomy 的率到年龄变化了。以两个都操作时间和流血和要求的输血体积的数量,阴道途径比所有另外的外科的途径优异。 | Jingjing Jiang Ting Ding Aiyue Luo Yunping Lu Ding Ma Shixuan Wang | 2014 | Frontiers of Medicine2014，8，4： | 2 |
| 6 | Production of human lysozyme-transgenic cloned porcine embryos by somatic nuclear transfer显示文摘Due to their physiology and organ size, pigs have significant potential as human disease models and as organ transplantation donors. Genetic modification of pigs could provide benefits for both agriculture and human medicine. In this study, five fetal pig fibroblast cell lines from two species (Wuzhishan and Landrace pigs) were transfected using double-marked human lysozyme (HLY) plasmids (pBC1-HLY-GFP-NEO) by a liposome-mediated method. The ratio of green fluorescent protein (GFP)-expressing cells was >95% in sw7, sw8, slw3 and slw6 cell lines, but only 49.3% in slw9 cells. Cells from the four highly transgenic lines were used as nuclear donors to construct embryos, which were then cultured after fusion and activation by electric stimulation. The rate of cleavage was 76.7%, 48 h after activation. After 7 days, 18.5% of cleaved eggs had developed to the blastocyst stage and 93.3% of blastocysts were GFP-positive. These results indicate that transgenic fetal pig flbroblast cell lines could be obtained by a liposome-mediated method, though the transfection efficiency varied between cell lines. Reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. | Qiuyan Li Hengxi Wei Ying Guo Yan Li Rui Zhao Yufang Ma Zhengquan Yu Bo Tang Lei Zhang Yunping Dai Ning Li | 2009 | Progress in Natural Science:Materials International2009，19，6： | 2 |
| 7 | Effect of tumor suppressor gene PTEN on the resistance to cisplatin in human ovarian cancer cell lines and related mechanisms显示文摘 | HuiJuan Wu Yang Cao Danhui Weng Hui Xing Xiaohong Song Jianfeng Zhou Gang Xu Yunping Lu Shixuan Wang Ding Ma | 2008 | Cancer Letters2008，，2： | 1 |
| 8 | Web Resources for Mass Spectrometry-based Proteomics显示文摘With the development of high-resolution and high-throughput mass spectrometry(MS)technology, a large quantum of proteomic data is continually being generated. Collecting and sharing these data are a challenge that requires immense and sustained human effort. In this report, we provide a classification of important web resources for MS-based proteomics and present rating of these web resources, based on whether raw data are stored, whether data submission is supported,and whether data analysis pipelines are provided. These web resources are important for biologists involved in proteomics research. | Tao Chen Jie Zhao Jie Ma Yunping Zhu | 2015 | Genomics, Proteomics & Bioinformatics2015，13，1： | 1 |
| 9 | Pirh2, a P53-Induced Ubiquitin-Protein Ligase, Promotes P53 Degradation 显示文摘 | Roger P Leng Yunping Lin Weili Ma | 2003 | Cell2003，112，： | 1 |
| 10 | Expression of pin1 and ki67 in cervical cancer and their significance显示文摘 | Li Hongyu Shen Hongling Xu Qian Deng Dongrui Wang Shixuan Lu Yunping Ma Ding | 2006 | Journal of Huazhong University of Science and Technology [Medical Sciences]2006，，： | 1 |
| 11 | A simplified algorithm for space vector modulation of threephase voltage source PWM rectifier显示文摘 | Lang Yunping Chen Huiming | 2004 | IEEE PESC2004，6，5： | 1 |
| 12 | An ANN and wavelet transformation based method for short term load forecast显示文摘 | Ma Ning Chen Yunping | 1998 | Proceedings of Energy Management and Power Delivery1998，，2： | 1 |
| 13 | Pathological features of COVID-19-associated liver injury-a preliminary proteomics report based on clinical samples显示文摘Dear Editor,Almost one-half of COVID-19 patients have symptoms of liver injury.However,the molecular characteristics in the COVID-19 liver remain unknown.Therefore,the need for long-term medication for recovery make it imperative to study the infectivity and pathogenicity of SARS-CoV-2 in the liver.Here,we have identifed signifcant proteomic changes for the first time in the liver of COVID-19 patients.We detected differential expression levels of several proteins that mediate characteristic cellular host responses elicited by SARS-CoV-2 infection in the liver. | Ling Leng Ruiyuan Cao Jie Ma Luye LV Wei Li Yunping Zhu Zhihong Wu Manli Wang Yiwu Zhou Wu Zhong | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 1 |
| 14 | A simplified algorithm for space vector modulation of three-phase voltage source PWM rectifier显示文摘 | Ma Hao Lang Yunping Chen Huiming | 2004 | IEEEPESC2004，6，5： | 1 |
| 15 | Reversal of multidrug resistance and inhibition of phosphorylation of AKT in human ovarian cancer cell line by wild-type PTEN gene显示文摘 | Huijuan Wu Danhui Weng Hui Xing Yunping Lu Ding Ma | 2008 | Journal of Huazhong University of Science and Technology2008，，6： | 1 |
| 16 | Serum proteome profiling provides a deep understanding of the‘gut-liver axis’in relation to liver injury and regeneration显示文摘The gut-liver axis is one of the major contributors to the transport of products from the intestine or intestinal microbes with the progression of liver regeneration.However,the influence of proteins from the hepatic portal vein(HPV),the bridge of enterohepatic circulation,on liver regeneration is unclear.For first time,we applied a quantitative proteomics approach to characterize the molecular pathology of the HPV sera of mice with antibiotic-induced intestinal flora disorder during acute liver injury.The biological processes of lipid metabolism and wound healing were enriched in the HPV of mice with intestinal flora disorder,whereas energy metabolism,liver regeneration,and cytoskeletal processes were downregulated.Moreover,95 and 35 proteins potentially promoting or inhibiting liver regeneration,respectively,were identified in HPV serum.Our findings will be beneficial to liver donors during liver transplantation. | Ling Leng Jie Ma Luye Lv Dunqin Gao Mansheng Li Yujie Wang Yunping Zhu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，3： | 1 |
| 17 | Effect of leptin on oocyte maturation and subsequent pregnancy rate of cloned embryos reconstructed by somatic cell nuclear transfer in pigs显示文摘由原子转移(SCNT ) 有的体的房间克隆猪在基本研究,人的药和农业生产的宽应用。到改善克隆效率,二基本成熟媒介的效果, NCSU-23 和 TCM199,被比较,并且 TCM199 为有 leptin 的下列实验被选择。我们系统地在 vitro 成熟(IVM ) 在卵母细胞上学习了 leptin 补充的效果,在激活的单性生殖的联盟者(PA ) 和 SCNT 胚胎的 vitro 开发并且在在胚胎转移(et ) 以后的 SCNT 胚胎的 vivo 开发。结果证明进成熟媒介的 100 或 200 ng/ml leptin 的补充极大地没影响 PA 和 SCNT 的卵母细胞,或劈开率的原子成熟(P >
0.05 ) 。当 100 或 200 ng/ml leptin 在 PA 胚囊被加到房间的成熟媒介,和数字时, PA 和 SCNT 胚胎的胚囊率显著地被改进也被改进(P <
0.05 ) 。在 SCNT 的胚囊的房间的数字被改进,当 100 ng/ml leptin 被增加时(P <
0.05 ) 。而且,到 IVM 媒介的 100 或 200 ng/ml leptin 的补充可以在猪克隆改进怀孕率和交货率。 | Hengxi Wei Qiuyan Li Jun Li Yan Li Yunping Dai Yufang Ma Kai Xue Ning Li | 2008 | Progress in Natural Science:Materials International2008，18，12： | 1 |
| 18 | A broadly neutralizing antibody against SARS-CoV-2 Omicron variant infection exhibiting a novel trimer dimer conformation in spike protein binding显示文摘Dear Editor,The constant evolution of SARS-CoV-2 has resulted in the emergence of circulating variants during the COVID-19 pandemic.Recently,a newly emerged B.1.1.529(Omicron)variant raised global concern.Compared with the original SARS-CoV-2 strain and other variants of concern(VOCs),the Omicron variant has more than 30 mutations on its spike(S)protein,of which 16 mutations are located in the receptor-binding domain(RBD). | Yingdan Wang Wuqiang Zhan Jiangyan Liu Yanqun Wang Xiang Zhang Meng Zhang Lin Han Yunping Ma Lu Lu Yumei Wen Zhenguo Chen Jincun Zhao Fan Wu Lei Sun Jinghe Huang | 2022 | Cell Research2022，32，9： | 1 |
| 19 | Production and verification of human papillomavirus type 18 vaccinein vitro显示文摘 | Shujie Liao Shixuan Wang Ling Xu Dongrui Deng Qian Xu Wei Wang Tao Zhu Xiangyang Bai Jianfeng Zhou Gang Xu Yunping Lu Li Meng Ding Ma | 2008 | Oncology Reports2008，，1： | 1 |
| 20 | An ANN and wavelet transformation based method for short term load forecast 显示文摘 | Chen Yunping | 1998 | Proceedings of Energy Management and Power Delivery1998，，2： | 1 |
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