| 1 | Exosomal transfer of microRNA-590-3p between renal tubular epithelial cells after renal ischemia-reperfusion injury regulates autophagy by targeting TRAF6显示文摘Background:Acute kidney injury(AKI)is a common complication in patients,especially elderly patients,who undergo cardiac surgery with cardiopulmonary bypass.Studies have indicated a protective role of autophagy in AKI.However,the mechanisms underlying the regulatory effect of autophagy in AKI among patients undergoing cardiac surgeries are poorly understood.In this study,we aimed to test the hypothesis that exosomal microRNAs(miRNAs)regulate autophagy in tubular epithelial cells after AKI.Methods:Plasma exosomal RNA was extracted from young and elderly AKI patients undergoing cardiac surgery,and the miRNAs expression during the perioperative period were analyzed using next-generation sequencing.The screened miRNAs and their target genes were subjected to gene oncology function and Kyoto Encyclopedia of Genes and Genome enrichment analyses.Renal tubular epithelial cell line(HK-2 cells)was cultured and hypoxia/reoxygenation(H/R)model was established,which is an in vitro renal ischemia/reperfusion(I/R)model.We used Western blot analysis,cell viability assay,transfection,luciferase assay to investigate the mechanisms underlying the observed increases in the levels of renal I/R injury-mediated exosomal miRNAs and their roles in regulating HK-2 cells autophagy.Results:miR-590-3p was highly enriched in the plasma exosomes of young AKI patients after cardiac surgery.Increased levels of miR-590-3p led to the increases in the expression of autophagy marker proteins,including Beclin-1 and microtubule associated protein 1 light chain 3 beta(LC3II),and prolonged the autophagic response in HK-2 cells after H/R treatment.These effects were achieved mainly via increases in the exosomal miR-590-3p levels,and the tumor necrosis factor receptor-associated factor 6 protein was shown to play a key role in I/R injury-mediated autophagy induction.Conclusion:Exosomes released from HK-2 cells after renal I/R injury regulate autophagy by transferring miR-590-3p in a paracrine manner,which suggests that increasing the miR-590-3p levels in HK-2 cell-derived exosomes may increase autophagy and protect against kidney injury after renal I/R injury. | Yimeng Chen Congya Zhang Yingjie Du Xiying Yang Min Liu Wenjing Yang Guiyu Lei Guyan Wang | 2022 | Chinese Medical Journal2022，135，20： | 1 |
| 2 | The Influence of Self-esteem on College Students’Learning Burnout:A Mediation Model显示文摘We measured 324 college students’self-esteem,motivation for achievement,and learning burnout,and also we examined the inner mechanism of self-esteem’s influence on students’learning burnout.The results show:(1)There is a negative effect of self-esteem on college students’learning burnout;(2)Motivation of pursuing succeed plays partial mediating effect between self-esteem and learning burnout,but mediating effect of avoiding failure motivation between them is not significant.The study reveals that:the degree of learning burnout can be reduced by improving college students’level of self-esteem. | MENG Xiangyin ZHANG Lei OU Guiyu | 2023 | Psychology Research2023，13，7： | 0 |
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