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| 1 | A novel artificial nerve graft for repairing longdistance sciatic nerve defects:a self-assembling peptide nanofiber scaffold-containing poly (lactic-co-glycolic acid) conduit显示文摘In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold(SAPNS)-containing poly(lactic-co-glycolic acid)(PLGA) conduit(SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury. | Xianghai Wang Mengjie Pan Jinkun Wen Yinjuan Tang Audra D.Hamilton Yuanyuan Li Changhui Qian Zhongying Liu Wutian Wu Jiasong Guo | 2014 | Neural Regeneration Research2014，9，24： | 5 |
| 2 | Protective effects of Buyinqianzheng Formula on mitochondrial morphology by PINK1/Parkin pathway in SH-SY5Y cells induced by MPP^(+)显示文摘Objective:Buyinqianzheng Formula(BYQZF)is clinically employed in traditional Chinese medicine to treat Parkinson's disease(PD)by improving mitochondrial dysfunction.However,the underlying mechanisms by which BYQZF affects mitochondrial morphology remain unknown.Therefore,we observed the effects of BYQZF on mitochondria from the perspective of the PINK1/Parkin pathway.Methods:Cell survival rates were assessed by Cell Counting Kit-8 assay.Expression levels of PINK1 and Parkin mRNA were examined by qRT-PCR.Protein expression levels of PINK1,PINK1-Ser228,Parkin,Parkin-Ser65,Drp1,and Drp1-Ser637 were examined by western blotting.PINK1,Parkin,and MitoTracker?Red CMXRos(MTR)were stained by triple-labeled immunofluorescence,and observed under laser confocal microscopy.Results:Cell survival rate,mitochondrial form factor,mean length and number of mitochondrial network branches,mitochondrial activity,m RNA expression levels of PINK1 and Parkin,and protein expression levels of PINK1,Parkin,and Drp1-Ser637 were reduced after 1-methyl-4-phenylpyridinium(MPP^(+))intervention.In contrast,Pearson's correlation coefficients between PINK1 and Parkin,and between Parkin and MTR,as well as protein expression levels of PINK1-Ser228,Parkin-Ser65,and Drp1 increased significantly after MPP^(+) intervention.Treatment with BYQZF increased cell survival rate,mitochondrial form factor,mean length and number of mitochondrial network branches,mitochondrial activity,m RNA expression levels of PINK1 and Parkin,and expression of PINK1,Parkin,and Drp1-Ser637 proteins.Pearson's correlation coefficients between PINK1 and Parkin,and between Parkin and MTR,as well as protein expression levels of PINK1-Ser228,Parkin-Ser65,and Drp1 decreased after BYQZF treatment.Conclusion:These results demonstrate that BYQZF has a protective effect on mitochondrial molecular mechanisms in the PD cell model,and the mechanism is related to the PINK1/Parkin pathway. | Haojie Ma Zhenyu Guo Cong Gai Cuicui Cheng Jinkun Zhang Yuxin Zhang Luping Yang Wandi Feng Yushan Gao Hongmei Sun | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，3： | 3 |
| 3 | Preparation and characterizations of tetragonal barium titanate powders by hydrothermal method显示文摘 | Xu Huarui Gao Lian Guo Jinkun | 2002 | J Eur Ceram Soc2002，22，： | 1 |
| 4 | Preparation and characterizations of tetragonal barium titanate powders by hydrothermal method显示文摘 | Huarui Xu Lian Gao Jinkun Guo | 2002 | Journal of the European Ceramic Society2002，22，7： | 1 |
| 5 | The Change Features of the West Boundary Bifurcation Line of the North Equatorial Current in the Pacific显示文摘The equatorial Current in the North Pacific(NEC) is an upper layer westward ocean current, which flows to the west boundary of the ocean, east of the Philippines, and bifurcates into the northerly Kuroshio and the main body of the southerly Mindanao current. Thus, NEC is both the south branch of the Subtropical Circulation and the north branch of the Tropical Circulation. The junction of the two branches extends to the west boundary to connect the bifurcation points forming the bifurcation line. The position of the North Pacific Equatorial Current bifurcation line of the surface determines the exchange between and the distribution of subtropical and tropical circulations, thus affecting the local or global climate. A new identification method to track the line and the bifurcation channel was used in this study, focusing on the climatological characteristics of the western boundary of the North Equatorial Current bifurcation line. The long-term average NEC west boundary bifurcation line shifts northwards with depth. In terms of seasonal variation, the average position of the western boundary of the bifurcation line is southernmost in June and northernmost in December, while in terms of interannual variation, from spring to winter in the years when ENSO is developing, the position of the west boundary bifurcation line of NEC is relatively to the north(south) in EI Ni?o(La Ni?a) years as compared to normal years. | GUO Junru LIU Yulong SONG Jun BAO Xianwen LI Yan CHEN Shaoyang YANG Jinkun | 2015 | Journal of Ocean University of China2015，14，6： | 0 |
| 6 | A highly conserved amino acid in high molecular weight glutenin subunit 1Dy12 contributes to gluten functionality and processing quality in wheat显示文摘Bread wheat(Triticum aestivum L.)is one of the most commonly consumed staples worldwide,with widespread uses in foods such as breads,noodles,cakes,and cookies(Veraverbeke and Delcour,2002).The specific cooking properties of wheat products are mainly conferred by the gluten proteins contained in wheat grains(Delcour et al.,2012).High molecular weight glutenin subunits(HMW-GSs)are important constituents of wheat gluten proteins,largely determining gluten elasticity and processing quality(Branlard and Dardevet,1985;Payne et al.,1988). | Changfeng Yang Qian Chen Mingming Xin Zhenqi Su Jinkun Du Weilong Guo Zhaorong Hu Jie Liu Huiru Peng Zhongfu Ni Qixin Sun Yingyin Yao | 2023 | Journal of Genetics and Genomics2023，50，11： | 0 |
| 7 | Proton pump inhibitors and stomach neoplasm显示文摘This study aimed to explore the relationship between proton pump inhibitors(PPIs)and gastric tumors and determine the reasons behind these connections.We reviewed studies on PPIs and stomach tumors.We explored the relationship between PPIs and different types of gastric neoplasms according to the classification of gastric neoplasms.Long-term use of PPIs is associated with stomach infection,high gastrin levels,and rebound acid hypersecretion,which are directly or indirectly related to the development of gastric neoplasms.PPIs can increase the risk of gastric fundal polyps.Further evidence is needed to prove that it can increase the risk of gastric cancer. | Jinkun Guo Zhongyin Zhou | 2020 | Oncology and Translational Medicine2020，6，1： | 0 |
| 8 | The construction of OPA1 overexpression plasmid and the cell model显示文摘The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected with the OPA1 overexpression plasmid by Lipo3000 transfection reagent in liposome transduction technology.A total of 3 groups were set up:the control group,negative control group,and OPA1 overexpression model group.RT-PCR technique was used to detect the expression of OPA1 at mRNA level.In addition,Western blot technique was used to detect the expression of OPA1 at protein level.We found that the mRNA and protein expression of OPA1 were significantly increased after transfection with OPA1 overexpression plasmid.These results highlight using liposome transduction technology,the OPA1 overexpression plasmid was successfully transfected into SH-SY5Y cells by Lipo3000 and the cell model of OPA1 gene overexpression was successully established. | Ma Haojie Gai Cong Feng Wandi Cheng Cuicui Zhou Mengqi Zhang Jinkun Zhang Yuxin Yang Luping Guo Zhenyu Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
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