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| 1 | Functional traits of tree species with phylogenetic signal co-vary with environmental niches in two large forest dynamics plots显示文摘Aims While using phylogenetic and functional approaches to test the mechanisms of community assembly,functional traits often act as the proxy of niches.However,there is little detailed knowledge regarding the correlation between functional traits of tree species and their niches in local communities.We suggest that the co-varying correlation between functional traits and niches should be the premise for using phylogenetic and functional approaches to test mechanisms of community assembly.Using functional traits,phylogenetic and environmental data,this study aims to answer the questions:(i)within local communities,do functional traits of co-occurring species covary with their environmental niches at the species level?and(ii)what is the key ecological process underlying community assembly in Xishuangbanna and Ailaoshan forest dynamic plots(FDPs)?Methods We measured seven functional traits of 229 and 36 common species in Xishuangbanna and Ailaoshan FDPs in tropical and subtropical China,respectively.We also quantified the environmental niches for these species based on conditional probability.We then analyzed the correlations between functional traits and environmental niches using phylogenetic independent contrasts.After examining phylogenetic signals of functional traits using Pagel’sλ,we quantified the phylogenetic and functional dispersion along environmental gradients within local tree communities.Important Findings For target species,functional traits do co-vary with environmental niches at the species level in both of the FDPs,supporting that functional traits can be used as a proxy for local-scale environmental niches.Functional traits show significant phylogenetic signals in both of the FDPs.We found that the phylogenetic and functional dispersion were significantly clustered along topographical gradients in the Ailaoshan FDP but overdispersion in the Xishuangbanna FDP.These patterns of phylogenetic and functional dispersion suggest that environmental filtering plays a key role in structuring local tree assemblages in Ailaoshan FDP,while competition exclusion plays a key role in Xishuangbanna FDP. | Jie Yang Xiuqin Ci Mengmeng Lu Guocheng Zhang Min Cao Jie Li Luxiang Lin | 2014 | Journal of Plant Ecology2014，7，2： | 16 |
| 2 | Contact fatigue life prediction of a bevel gear under spectrum loading显示文摘Rolling contact fatigue(RCF)issues,such as pitting,might occur on bevel gears because load fluctuation induces considerable subsurface stress amplitudes.Such issues can dramatically affect the service life of associated machines.An accurate geometry model of a hypoid gear utilized in the main reducer of a heavy-duty vehicle is developed in this study with the commercial gear design software MASTA.Multiaxial stress–strain states are simulated with the finite element method,and the RCF life is predicted using the Brown–Miller–Morrow fatigue criterion.The patterns of fatigue life on the tooth surface are simulated under various loading levels,and the RCF S–N curve is numerically generated.Moreover,a typical torque–time history on the driven axle is described,followed by the construction of program load spectrum with the rain flow method and the Goodman mean stress equation.The effects of various fatigue damage accumulation rules on fatigue life are compared and discussed in detail.Predicted results reveal that the Miner linear rule provides the most optimistic result among the three selected rules,and the Manson bilinear rule produces the most conservative result. | Pan JIA Huaiju LIU Caichao ZHU Wei WU Guocheng LU | 2020 | Frontiers of Mechanical Engineering2020，15，1： | 2 |
| 3 | Carboxylate-induced Various Structures of Ni(II) Complexes with Fluorescence Sensing and Bifunctional Electrochemical Properties显示文摘We synthesized three new Ni(II) coordination polymers [Ni(LXHLPXH2O)2]- H2O(CP1),[Ni(LXNIP)]-2H2O(CP2) and [Ni(LXNDCXH20)2](CP3)[L=N,N′-bis(pyridme-3-yl)thiophene-2,5-dicarboxamide, H2HIP=5-hydroxyisophthalic acid, H2NIP=5-nitroisophthalic acid, H2NDC=2,6-naphthalenedicarboxylie acid] by hydrothermal method, which were characterized by means of infrared spectra(IR), TG analyses, PXRD and single-crystal X-ray diffraction. The CPI is a ID tubular structure based on [Ni-HIP]2 loops and pairs of L ligands. CP2 is a 2D 3,5-connected architecture, which consists of Ni-L linear chains and (Ni-NIP)2 double chains. CP3 is a 2D network, which features 4-connected topology. Solid-state luminescent behaviours of CP-P3 were investigated. The CPI can detect Fe^3+ ions through luminescence quenching. The electrochemical properties of CPI buk-modified carbon paste electrode(CP1-CPE) has also been investigated, which has bifunctional electrocatalytic activity for oxidation of ascorbic acid and reduction of NO2^-. | LU Xue LIU Guocheng WANG Xiang LIN Hongyan WANG Xiuli | 2019 | Chemical Research in Chinese Universities2019，35，4： | 2 |
| 4 | Interactions between Sulfa Drug Sulfadiazine and Hydrophobic Talc Surfaces 显示文摘 | LU Guocheng LI Zhaohui Hoeppner N | 2014 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2014，446，5： | 1 |
| 5 | Tumor Necrosis Factor-alpha Promotes Tumor Growth by Inducing Vascular Endothelial Growth Factor显示文摘 | Yingying Jing Nannan Ma Tingting Fan Chenyang Wang Xinxin Bu Guocheng Jiang Rong Li Lu Gao Ding Li Mengchao Wu Lixin Wei | 2011 | Cancer Investigation2011，，7： | 1 |
| 6 | Metabolic engineering of Corynebacterium glutamicum S9114 based on whole-genome sequencing for efficient N-acetylglucosamine synthesis显示文摘Glucosamine(GlcN)and its acetylated derivative N-acetylglucosamine(GlcNAc)are widely used in the pharmaceutical industries.Here,we attempted to achieve efficient production of GlcNAc via genomic engineering of Corynebacterium glutamicum.Specifically,we ligated the GNA1 gene,which converts GlcN-6-phosphate to GlcNAc-6-phosphate by transferring the acetyl group in Acetyl-CoA to the amino group of GlcN-6-phosphate,into the plasmid pJYW4 and then transformed this recombinant vector into the C.glutamicum ATCC 13032,ATCC 13869,ATCC 14067,and S9114 strains,and we assessed the GlcNAc titers at 0.5 g/L,1.2 g/L,0.8 g/L,and 3.1 g/L from each strain,respectively.This suggested that there were likely to be significant differences among the key genes in the glutamate and GlcNAc synthesis pathways of these C.glutamicum strains.Therefore,we performed whole genome sequencing of the S9114 strain,which has not been previously published,and found that there are many differences among the genes in the glutamate and GlcNAc synthesis pathways among the four strains tested.Next,nagA(encoding GlcNAc-6-phosphate deacetylase)and gamA(encoding GlcN-6-phosphate deaminase)were deleted in C.glutamicum S9114 to block the catabolism of intracellular GlcNAc,leading to a 54.8%increase in GlcNAc production(from 3.1 to 4.8 g/L)when grown in a shaker flask.In addition,lactate synthesis was blocked by knockout of ldh(encoding lactate dehydrogenase);thus,further increasing the GlcNAc titer to 5.4 g/L.Finally,we added a key gene of the GlcN synthetic pathway,glmS,from different sources into the expression vector pJYW-4-ceN,and the resulting recombinant strain CGGN2-GNA1-CgglmS produced the GlcNAc titer of 6.9 g/L.This is the first report concerning the metabolic engineering of C.glutamicum,and the results of this study provide a good starting point for further metabolic engineering to achieve industrial-scale production of GlcNAc. | Chen Deng Xueqin Lv Yanfeng Liu Jianghua Li Wei Lu Guocheng Du Long Liu | 2019 | Synthetic and Systems Biotechnology2019，4，3： | 0 |
| 7 | In silico cell factory design driven by comprehensive genome‑scale metabolic models:development and challenges显示文摘Genome-scale metabolic models(GEMs)have been widely used to design cell factories in silico.However,initial flux balance analysis only considers stoichiometry and reaction direction constraints,so it cannot accurately describe the distribution of metabolic flux under the control of various regulatory mechanisms.In the recent years,by introducing enzymology,thermodynamics,and other multiomics-based constraints into GEMs,the metabolic state of cells under different conditions was more accurately simulated and a series of algorithms have been presented for microbial phenotypic analysis.Herein,the development of multiconstrained GEMs was reviewed by taking the constraints of enzyme kinetics,thermodynamics,and transcriptional regulatory mechanisms as examples.This review focused on introducing and summarizing GEMs application tools and cases in cell factory design.The challenges and prospects of GEMs development were also discussed. | Jiangong Lu Xinyu Bi Yanfeng Liu Xueqin Lv Jianghua Li Guocheng Du Long Liu | 2023 | Systems Microbiology and Biomanufacturing2023，3，2： | 0 |
| 8 | Effect of reduction-oxidation treatment on structure and catalytic properties of ordered mesoporous Cu-Mg-Al composite oxides显示文摘Ordered mesoporous Cu–Mg–Al composite oxides were synthesized via the one-pot evaporation-induced self-assembly strategy. Using this method, copper was first homogeneously incorporated into the ordered mesoporous spinel matrix. After H2 reduction treatment,according to X-ray diffraction(XRD) and transmission electron microscopy(TEM) results, copper existed as metallic nanoparticles with the size of 6–10 nm that well decorated the parent mesoporous skeleton. The metallic nanoparticles were then re-oxidized to copper oxide when exposed to air or during CO oxidation reaction at low temperatures. Thus, copper migrated from bulk spinel phase to the surface after the reduction–oxidation treatment. Moreover, the copper on the surface was re-incorporated into the bulk spinel phase by further thermaltreatment at much higher temperature in the presence of air. The correlation between the state of copper in the mesoporous composite oxides and the catalytic performance toward CO oxidation was studied. It was found that copper existed as oxide nanoparticles on the surface of mesoporous Mg–Al skeleton is much more active than that existed as lattice Cu ions in spinel phase. | Jingting Lu Yan Zhang Chengli Jiao Suresh Kumar Megarajan Dong Gu Guocheng Yang Heqing Jiang Chunjiang Jia Ferdi Schiith | 2015 | Science Bulletin2015，60，12： | 0 |
| 9 | Chitin deacetylase:from molecular structure to practical applications显示文摘Chitosan and chitooligosaccharides(COS),as derivatives of chitin through deacetylation reaction,have broad applications due to their good biodegradability,biocompatibility,and solubility.In addition,chitosan and COS are involved in cell wall morphogenesis and host–pathogen interactions in vivo.Chitin deacetylases(CDAs)are enzymes that can catalyze the deN-acetylation of chitin.They are widely distributed in protozoa,algae,bacteria,fungi,and insects with important physiological functions.Compared with the traditional chemical method,enzymatic catalysis by CDAs provides an enzymatic catalysis method to produce chitosan and COS with controllable deacetylation site and environmental friendliness.These characteristics attract researchers to produce CDAs by fungicides or pesticides.However,researches on heterologous expression and directed evolution of CDAs are still lacking.In this review,we summarize the latest knowledge of CDAs,especially for heterologous expression systems and directed evolution strategies,which may contribute to the industrial production and future application of CDAs. | Ziyang Huang Xueqin Lv Guoyun Sun Xinzhu Mao Wei Lu Yanfeng Liu Jianghua Li Guocheng Du Long Liu | 2022 | Systems Microbiology and Biomanufacturing2022，2，2： | 0 |
| 10 | Chemical and spectroscopic characteristics of humic acid from a clay loam soil in Ontario after 52 years of consistent fertilization and crop rotation显示文摘Long-term fertilization and crop rotation can influence both organic C sequestration as well as the C composition of soils and the more resistant organic C compounds contained in humic acid(HA). This study examined the effects of fertilization and cropping type(monoculture corn(MC) and Kentucky bluegrass sod(KBS) and corn-oat-alfalfa-alfalfa rotation(RC)) on the HA composition of soil from a 52-year field study in southern Ontario, Canada. Humic acid samples were extracted from soil, and elemental analysis, infrared spectroscopy, solid state 13C nuclear magnetic resonance spectra, and electron paramagnetic resonance methods were used to determine the influence of the cropping type on the characteristics of HA. Both fertilization and cropping type affected the chemical characteristics of HA. Fertilization led to a 5.9% increase in C, a 7.6% decrease in O, and lower O/C and(N + O)/C ratios in HA as compared to the corresponding non-fertilized treatments. Rotation resulted in a lower proportion of C(48.1%) and a greater(N + O)/C ratio(0.7) relative to monoculture cropping. Infrared spectroscopy analysis showed that HA contained more C-O groups in fertilized soil than in non-fertilized soil under MC and KBS. Fertilization increased the O-alkyl-C, phenolic-C, and free radical contents of HA relative to non-fertilization treatments. Rotation decreased the aliphatic and carboxyl groups and increased the O-alkyl, carbohydrate, aryl, and phenolic groups and free radicals, relative to MC and KBS. Both long-term crop rotation and fertilization dramatically modified the soil HA composition. Significant relationships were observed between the molecular composition of HA and soil organic C. Hence, humic acid characterization could be used as an indicator of the long-term sustainability of crop management practices. | Nan ZHAO Xixiang YANG Guocheng HUANG Yizhong LU Jing ZHANG Yuanyuan FAN Craig F.DRURYS Xueming YANG | 2021 | Pedosphere2021，31，1： | 0 |
| 11 | Implementing geostatistical analysis to study spatio-temporal distribution patterns of swimming crabs(Portunus trituberculatus)显示文摘This study attempted to compare the performance of local polynomial interpolation,inverse distance weighted interpolation,and ordinary kriging in studying distribution patterns of swimming crabs.Cross-validation was used to select the optimum method to get distribution results,and kriging was used for making spatial variability analysis.Data were collected from 87 sampling stations in November of 2015(autumn)and February(winter),May(spring)and August(summer)of 2016.Results indicate that swimming crabs widely distributed in autumn and summer:in the summer,they were more spatially independent,and resources in each sampling station varied a lot;in the winter and spring,the abundance of crabs was much lower,but the individual crab size was bigger,and they showed the patchy and more concentrative distribution pattern,which means they were more spatially dependent.Distribution patterns were in accordance with ecological migration features of swimming crabs,which were affected by the changing marine environment.This study could infer that it is applicable to study crab fishery or even other crustacean species using geostatistical analysis.It not only helps practitioners have a better understanding of how swimming crabs migrate from season to season,but also assists researchers in carrying out a more comprehensive assessment of the fishery.Therefore,it may facilitate advancing the implementation in the pilot quota management program of swimming crabs in northern Zhejiang fishing grounds. | Wenbin Zhu Kaner Lu Zhanhui Lu Qian Dai Zhenghua Li Yongdong Zhou Siqi Huang Haichen Zhu Guocheng Cui | 2021 | Acta Oceanologica Sinica2021，40，6： | 0 |
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