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| 1 | Cell responses of Dunaliella salina FACHB 435 (Green Alga) to microgravitational stimulation by clinorotation显示文摘Clinorotation experiments were established to simulate microgravity on ground. It was found that there were obvious changes of Dunaliella salina FACHB435 cells and their metabolic characteristics during clinorotation. The changes included the increases of glycerol content, the rate of H + secretion and PM H + ATPase activity, and the decrease of ratio of the plasma membrane (PM) phospholipid to PM protein. These results indicated that microgravity was a stress environment to Dunaliella salina . It is deduced that it would be possible to attribute the effect of microgravity on algal cells to the secondary activation of water stress. | Zhangli Hu Yongding Liu | 1998 | Chinese Science Bulletin1998，43，20： | 3 |
| 2 | Expression of bkt and bch genes from Haematococcus pluvialis in transgenic Chlamydomonas显示文摘β-carotene ketolase andβ-carotene hydroxylase encoded by bkt and bch,respectively,are key enzymes required for astaxanthin biosynthesis in Haematococcu pluvialis 34-1n.Two expression vectors containing cDNA sequences of bkt and bch were constructed and co-transformed into cell-wall-deficient Chlamydomonas reinhardtii CC-849.Transgenic algae were screened on TAP agar plates containing 10μg mL-1 Zeomycin.PCR-Southern analysis showed that bkt and bch were integrated into the genomes of C.reinhardtii.Transcripts of bkt and bch were further confirmed by RT-PCR-Southern analysis.Compared with the wild type,transgenic algae produced 29.04%and 30.27%more carotenoids and xanthophylls,respectively.Moreover,the transgenic algae could accumulate 34%more astaxanthin than wild type.These results indicate that foreign bkt and bch genes were successfully translated intoβ-carotene ketolase andβ-carotene hydroxylase,which were responsible for catalyzing the biosynthesis of astaxanthin in transgenic algae. | ZHENG KaiJing WANG ChaoGang XIAO Ming CHEN Jun LI JianCheng HU ZhangLi | 2014 | Science China(Life Sciences)2014，57，10： | 1 |
| 3 | Removal of fluoranthene and pyrene by different microalgal species显示文摘 | LEI Anping HU Zhangli WONG Yukshan | 2007 | Bioresource Technology2007，98，2： | 1 |
| 4 | Stable expression of antibiotic-resistant gene ble from Streptoalloteichus hindustanus in the mitochondria of Chlamydomonas reinhardtii显示文摘 | Hu Zhangli Fan Zhun Zhao Zhonglin | 2012 | PLOS One2012，7，35： | 1 |
| 5 | Heterotrophic bacteria of the Dapeng Bay in the South China Sea: community structure,abundance,and the relationships of culturablity with environmental factors显示文摘The community composition,abundance and culturablity of heterotrophic bacterioplankton in the Dapeng Bay of the South China Sea were investigated by morphological method,bacterial cultivation and biochemical methods over an annual cycle.Aeromonas and Pseudomonas were the predominant genera among the Gram-negative bacteria.The bacterioplankton abundance fluctuated from 2.07×10 8 to 2.44×10 9 cells/dm 3 ,and culturable bacteria abundance ranged between 0.1×10 6 and 16.07×10 6 CFU/dm 3 .The Culturablity of heterotrophic bacteria ranged between 0.01% and 1.31%.The relationship between bacterial culturablity and the environmental factors was also studied in different sites of the Dapeng Bay.Throughout the bay,a significantly positive relationship was evidenced between bacterial culturability and temperature,meanswhile bacterial culturability was significantly inversely related with salinity.The positive relationship was shown at the central bay while a negative relationship was observed at the bay mouth and there was no significant correlation at top bay between bacterial culturablity and chlorophyll a of sea water.A positive relationship was found between culturability and DIN all the bay,but DIP showed a significantly negative relationship with bacterial culturability only at the mouth of the bay. | JIANG Fajun HU Zhangli HU Chaoqun | 2010 | Acta Oceanologica Sinica2010，29，4： | 1 |
| 6 | Comprehensive study on the total dose effects in a 180 nm CMOS technology显示文摘 | Hu Zhiyuan Liu Zhangli Shao Hua | 2011 | IEEE Trans on Nucl Sei2011，58，3： | 1 |
| 7 | Distinct roles of alternative oxidase pathway during the greening process of etiolated algae显示文摘The vital function of mitochondrial alternative oxidase(AOX) pathway in optimizing photosynthesis during plant de-etiolation has been well recognized. However, whether and how AOX impacts the chloroplast biogenesis in algal cells remains unclear. In the present study, the role of AOX in regulating the reassembly of chloroplast in algal cells was investigated by treating Auxenochlorella protothecoides with salicylhydroxamic acid(SHAM), the specific inhibitor to AOX, in the heterotrophy to autotrophy transition process. Several lines of evidences including delayed chlorophyll accumulation, lagged reorganization of chloroplast structure, altered PSI/PSII stoichiometry, and declined photosynthetic activities in SHAM treated cells indicated that the impairment in AOX activity dramatically hindered the development of functioning chloroplast in algal cells. Besides, the cellular ROS levels and activities of antioxidant enzymes were increased by SHAM treatment, and the perturbation on the balance of NAD+/NADH and NADP+/NADPH ratios was also observed in A. protothecoides lacking AOX activity, indicating that AOX was essential in promoting ROS scavenging and keeping the redox homeostasis for algal chloroplast development during greening. Overall, our study revealed the essentiality of mitochondrial AOX pathway in sustaining algal photosynthetic performance and provided novel insights into the physiological roles of AOX on the biogenesis of photosynthetic organelle in algae. | Ying Liu Hua Zhang Yan Cui Yanli Zheng Hui Chen Zhangli Hu Qingyu Wu | 2021 | Science China(Life Sciences)2021，64，5： | 0 |
| 8 | Regenerating Plants from Cryopreserved Adventitious Buds of Haploids in Rice显示文摘0IntroductionThedevelopmentofcryopreservationhasof-feredanewwayoflong-termpreservationofplantgermplasms.SinceNagandStreet(197... | Zhang, Zhihong Hu, Zhangli | 1999 | Wuhan University Journal of Natural Sciences1999，4，1： | 0 |
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