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| 1 | Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems显示文摘The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM(available at http://gffzz103f3ce5d9b14419hwwnnkxuxwkfo6kn9.ffgz.tsg.suse.edu.cn/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing(NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers,cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems. | Qing Liu Chun Wang Xiaozhen Jiao Huawei Zhang Lili Song Yanxin Li Caixia Gao Kejian Wang | 2019 | Science China(Life Sciences)2019，62，1： | 37 |
| 2 | The endoplasmic reticulum-associated degradation is necessary for plant salt tolerance显示文摘真核细胞的有机体有允许错误褶层或 unassembled 蛋白质在 endoplasmic 蜂窝胃被保留的质量控制的机制(嗯) 并且随后由嗯联系降级(ERAD ) 降级了。ERAD 小径很好在酵母和哺乳动物被学习;然而,植物 ERAD 的生物功能没被报导。通过分子、细胞的生物途径,我们发现 ERAD 是必要的让植物克服盐应力。在蛋白质在植物房间增加了的盐处理 ubiquitinated 之上,特别展开了快速在 ER 积累了并且随后导致了的蛋白质嗯压力回答。在 ERAD 小径的 HRD1/HRD3 建筑群的 HRD3A 的缺点导致了展开的蛋白质反应(UPR ) 的改变,腌的增加的植物敏感,和在植物房间的 ERAD 底层的保留。而且,我们证明从 ER 的那个 Ca 2+ 版本涉及 UPR 的举起,反应的氧种类(ROS ) 参与 ERAD 相关的植物盐反应小径。 | Lijing Liu Feng Cui Qingliang Li Bojiao Yin Huawei Zhang Baoying Lin Zaorong Wu Ran Xia Sanyuan Tang Qi Xie | 2011 | Cell Research2011，21，6： | 19 |
| 3 | Manipulating mRNA splicing by base editing in plants显示文摘Precursor-mRNAs(pre-mRNA) can be processed into one or more mature m RNA isoforms through constitutive or alternative splicing pathways. Constitutive splicing of pre-mRNA plays critical roles in gene expressional regulation, such as intronmediated enhancement(IME), whereas alternative splicing(AS) dramatically increases the protein diversity and gene functional regulation. However, the unavailability of mutants for individual spliced isoforms in plants has been a major limitation in studying the function of mRNA splicing. Here, we describe an efficient tool for manipulating the splicing of plant genes. Using a Cas9-directed base editor, we converted the 5′ splice sites in four Arabidopsis genes from the activated GT form to the inactive AT form. Silencing the AS of HAB 1.1(encoding a type 2 C phosphatase) validated its function in abscisic acid signaling, while perturbing the AS of RS31 A revealed its functional involvement in plant response to genotoxic treatment for the first time. Lastly,altering the constitutive splicing of Act2 via base editing facilitated the analysis of IME. This strategy provides an efficient tool for investigating the function and regulation of gene splicing in plants and other eukaryotes. | Chenxiao Xue Huawei Zhang Qiupeng Lin Rong Fan Caixia Gao | 2018 | Science China(Life Sciences)2018，61，11： | 18 |
| 4 | A Research Review on the Key Technologies of Intelligent Design for Customized Products显示文摘 | Shuyou Zhang Jinghua Xu Huawei Gou Jianrong Tan | 2017 | Engineering2017，3，5： | 14 |
| 5 | Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene显示文摘Maize(Zea mays L.)is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013(Baldaufa et al.,2016).Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s(Duvick,2001).The high-yield maize varieties used today are all hybrids,and the production of hybrid maize seed requires sterilization to prevent self-pollination.Conventional seed produc- | Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling Chen Jinxing Liu Huabang Chen Caixia Gao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 6 | Investigation of the Anisotropic Morphology-Induced Effects of the Slippery Zone in Pitchers of Nepenthes alata显示文摘 | Pengfei Zhang Huawei Chen Deyuan Zhang | 2015 | Journal of Bionic Engineering2015，12，1： | 13 |
| 7 | High-precision bio-replication of synthetic drag reduction shark skin显示文摘Nano-long chains were grafted over the replicated micro-grooves of shark skin in a novel attempt to replicate bio-synthetic drag reduction structure with high precision through synthetic bio-replication. Pre-treated shark skin was used as casting template to prepare a flexible female die of silicone rubber by soft die formation. A waterborne epoxy resin was then used to graft long-chains of drag reduction agent and prepare a synthetic drag reduction shark skin with nano-long chain drag reduction interface and lifelike micro-grooves. Replication precision analysis shows that this technology could replicate the complicated three-dimensional morphology of a biological drag reduction surface with high precision. Drag reduction experiments show that the material had an excellent synthetic drag reduction effect, with a maximal drag reduction rate of up to 24.6% over the velocities tested. | ZHANG DeYuan LI YuanYue HAN Xin LI Xiang CHEN HuaWei | 2011 | Chinese Science Bulletin2011，56，9： | 13 |
| 8 | Mercury oxidation and adsorption characteristics of potassium permanganate modified lignite semi-coke显示文摘The adsorption characteristics of virgin and potassium permanganate modified lignite semi-coke(SC)for gaseous Hg0 were investigated in an attempt to produce more effective and lower price adsorbents for the control of elemental mercury emission.Brunauer-EmmettTeller(BET)measurements,X-ray powder diffraction(XRD)and X-ray photoelectron spectroscopy(XPS)were used to analyze the surface physical and chemical properties of SC,Mn-SC and Mn-H-SC before and after mercury adsorption.The results indicated that potassium permanganate modification had significant influence on the properties of semi-coke,such as the specific surface area,pore structure and surface chemical functional groups.The mercury adsorption efficiency of modified semi-coke was lower than that of SC at low temperature,but much higher at high temperature.Amorphous Mn7+,Mn6+ and Mn 4+ on the surface of Mn-SC and Mn-H-SC were the active sites for oxidation and adsorption of gaseous Hg0,which oxidized the elemental mercury into Hg2+ and captured it.Thermal treatment reduced the average oxidation degree of Mnx+ on the surface of Mn-SC from 3.80 to 3.46.However,due to the formation of amorphous MnOx,the surface oxidation active sites for gaseous Hg0 increased,which gave Mn-H-SC higher mercury adsorption efficiency than that of Mn-SC at high temperature. | Huawei Zhang Jitao Chen Peng Liang Li Wang | 2012 | Journal of Environmental Sciences2012，24，12： | 12 |
| 9 | Biomimetic Drag Reduction Study on Herringbone Riblets of Bird Feather显示文摘 | Huawei Chen Fugang Rao Xiaopeng Shang Deyuan Zhang Ichiro Hagiwara | 2013 | Journal of Bionic Engineering2013，10，3： | 12 |
| 10 | Myelin protein zero and its antibody in serum as biomarkers of n-hexane-induced peripheral neuropathy and neurotoxicity effects显示文摘 | Jia Xiaowei Liu Qingjun Zhang Yanshu Dai Yufei Duan Huawei Bin Ping Niu Yong Liu Jie Zhong Liuzhen Guo Jisheng Liu Xiaofeng Zheng Yuxin | 2014 | Chinese Medical Journal2014，，8： | 8 |
| 11 | A novel mutation in the MITF may be digenic with GJB2 mutations in a large Chinese family of Waardenburg syndrome type Ⅱ显示文摘Waardenburg syndrome typeⅡ(WS2) is associated with syndromic deafness.A subset of WS2,WS2A,accounting for approximately 15%of patients,is attributed to mutations in the microphthalmia-associated transcription factor(MITF) gene.We examined the genetic basis of WS2 in a large Chinese family.All 9 exons of the MITF gene,the single coding exon(exon 2) of the most common hereditary deafness gene GJB2 and the mitochondrial DNA(mtDNA) 12S rRNA were sequenced.A novel heterozygous mutation c.[742_743delAAinsT;746_747delCA]in exon 8 of the MITF gene co-segregates with WS2 in the family.The MITF mutation results in a premature termination codon and a truncated MITF protein with only 247 of the 419 wild type amino acids.The deaf proband had this MITF gene heterozygous mutation as well as a c.[109G>A]+ [235delC]compound heterozygous pathogenic mutation in the GJB2 gene.No pathogenic mutation was found in mtDNA 12S rRNA in this family.Thus,a novel compound heterozygous mutation,c.[742_743delAAinsT;746_747delCA]in MITF exon 8 was the key genetic reason for WS2 in this family,and a digenic effect of MITF and GJB2 genes may contribute to deafness of the proband. | Xukun Yan Tianyu Zhang Zhengmin Wang Yi Jiang Yan Chen Hongyan Wang Duan Ma Lei Wang Huawei Li | 2011 | Journal of Genetics and Genomics2011，38，12： | 8 |
| 12 | Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded βC1显示文摘西红柿黄叶卷屈中国联系病毒的 betasatellite 编码的 C1 蛋白质作为一个致病力决定因素工作。更好理解 C1 在致病力工作的分子的基础,酵母一个烟草 cDNA 图书馆的二混血儿的屏幕作为诱饵用 C1 被执行。屏幕表明 C1 与烟草戒指手指蛋白质交往指明了 NtRFP1,它被双分子的荧光互补和 co-immunoprecipitation 试金进一步在 Nicotiana benthamiana 房间证实。NtRFP1 的表示被 C1 导致,并且在 vitro ubiquitination,试金证明 NtRFP1 是调停的功能的 E3 ubiquitin ligase C1 ubiquitination。另外, C1 被显示是在 vivo 的 ubiquitinated 并且由植物 26S proteasome 降级。在病毒的感染以后,植物 overexpressing NtRFP1 开发了稀释症状,而有 NtRFP1 的 silenced 表示的植物显示出严重症状。证据的另外的线证明 NtRFP1 通过由 26S proteasome 支持它的降级稀释导致 C1 的症状。一起拿,我们的结果建议烟草戒指 E3 ligase NtRFP1 由与 C1 交往调停稀释疾病症状它经由 ubiquitin/26S proteasome 系统的 ubiquitination 和降级。 | Qingtang Shen Tao Hu Min Bao Linge Cao Huawei Zhang Fengmin Song Qi Xie Xueping Zhou | 2016 | Molecular Plant2016，9，6： | 8 |
| 13 | Experimental investigation on a high subsonic compressor cascade flow显示文摘With the aim of deepening the understanding of high-speed compressor cascade flow,this paper reports an experimental study on NACA-65 K48 compressor cascade with high subsonic inlet flow.With the increase of passage pressurizing ability, endwall boundary layer behavior is deteriorated, and the transition zone is extended from suction surface to the endwall as the adverse pressure gradient increases.Cross flow from endwall to midspan, mixing of corner boundary layer and the main stream, and reversal flow on the suction surface are caused by corner separation vortex structures.Passage vortex is the main corner separation vortex.During its movement downstream, the size grows bigger while the rotating direction changes, forming a limiting circle.With higher incidence, corner separation is further deteriorated, leading to higher flow loss.Meanwhile, corner separation structure, flow mixing characteristics and flow loss distribution vary a lot with the change of incidence.Compared with low aspect-ratio model, corner separation of high aspect-ratio model moves away from the endwall and is more sufficiently developed downstream the cascade.Results obtained present details of high-speed compressor cascade flow,which is rare in the relating research fields and is beneficial to mechanism analysis, aerodynamic optimization and flow control design. | Zhang Haideng Wu Yun Li Yinghong Lu Huawei | 2015 | Chinese Journal of Aeronautics2015，28，4： | 8 |
| 14 | A Robust Collaborative Recommendation Algorithm Based on k-distance and Tukey M-estimator显示文摘The existing collaborative recommendation algorithms have lower robustness against shilling attacks.With this problem in mind,in this paper we propose a robust collaborative recommendation algorithm based on k-distance and Tukey M-estimator.Firstly,we propose a k-distancebased method to compute user suspicion degree(USD).The reliable neighbor model can be constructed through incorporating the user suspicion degree into user neighbor model.The influence of attack profiles on the recommendation results is reduced through adjusting similarities among users.Then,Tukey M-estimator is introduced to construct robust matrix factorization model,which can realize the robust estimation of user feature matrix and item feature matrix and reduce the influence of attack profiles on item feature matrix.Finally,a robust collaborative recommendation algorithm is devised by combining the reliable neighbor model and robust matrix factorization model.Experimental results show that the proposed algorithm outperforms the existing methods in terms of both recommendation accuracy and robustness. | YI Huawei ZHANG Fuzhi LAN Jie | 2014 | China Communications2014，11，9： | 6 |
| 15 | A pair of transposon-derived proteins function in a histone acetyltransferase complex for active DNA demethylation显示文摘Transposons 被 epigenetic 机制通常包括 DNA methylation 使沉默。这里,我们识别了一双先驱导出 transposon 的蛋白质(HDP ) , HDP1 和 HDP2,在 Arabidopsis 的反沉默因素。hdp1 和 hdp2 异种显示了 transgenes 和某 transposons 的提高的 silencing。种系发生的分析表明 HDP1 和 HDP2 分别地从先驱编码 transposon 的 transposase 和 DNA 有约束力的蛋白质被共同驯养。HDP1 在原子核与 HDP2 交往,类似于他们的 transposon 对应物。而且, HDP1 和 HDP2 与组成在 DNA demethylation 工作的 histone acetyltransferase 建筑群的 IDM1, IDM2, IDM3 和 MBD7 被联系。HDP2 和 methyl-DNA-binding 蛋白质 MBD7 分享普通 genomic 绑定地点的一个大集合,显示他们联合决定 histone acetyltransferase 建筑群的目标特性。因此,我们的数据表明 HDP1 和 HDP2 组成被招募了到 histone acetyltransferase 建筑群阻止 DNA hypermethylation 和 epigenetic silencing 的一个功能的模块。 | Cheng-Guo Duan Xingang Wang Shaojun Xie Li Pan Daisuke Miki Kai Tang Chuan-Chih Hsu Mingguang Lei Yingli Zhong Yueh-Ju Hou ZhijuanWang Zhengjing Zhang Satendra K Mangrauthia Huawei Xu Heng Zhang Brian Dilkes W Andy Tao Jian-Kang Zhu | 2017 | Cell Research2017，27，2： | 6 |
| 16 | Tectonic and Environmental Evolutions of the Northern Tibetan Plateau Prior to the Collision of India with Asia显示文摘Tectonic and environmental patterns and evolution of the present North Tibetan Plateau(NTP) prior to the India collision with Asia is significant to understand the formation of the Tibetan Plateau and its influence on the environment. In this study, we integrated and analyzed the tectonostratigraphy and the special sedimentary layers whose climatic implications are clear in the NTP. Additionally, we stressed the tectonic and environmental events and their evolutions from the Mesozoic to the Early Cenozoic. Our results show that four tectonic phases, which sequentially took place during the Triassic, Jurassic, Cretaceous and Paleogene, played an important role on the formation of the North Tibet. The climate was basically dry and hot from the Triassic to the Eocene and became dry and cool since the Oligocene in this region. The climatic evolution was characterized by a transition from a wet and hot phase during the Triassic- Middle Jurassic, to a dry and hot phase during the Late Jurassic- Eocene. Both phases encompassed 5 wet and hot periods followed by 5 dry and hot climate events, respectively. In addition, we found that the tectonic deformation and the climatic conditions were spatially and temporally different. In detail, in the regions north of the PaleoTian Shan and Paleo-Qilian Mts. the tectonic deformation and climatic condition were stronger and wetter than in regions south of the Paleo-Tian Shan and Paleo-Qilian Mts. during the Late Triassic – Jurassic. Whereas in the Cretaceous, the tectonic movement was intensive in the west but steady in the east, and climate was dry in the south but wet in the north of NTP. The formation of the tectonic and climatic patterns in NTP were the consequence of either global climate change or regional tectonics, including the Paleo-Asian Ocean closure and the Qiangtang block, Lhasa block and India plate collision subsequently to Asia. Furthermore, the regional tectonic events occurred before any global climate change and drove the climatic change in the NTP. | DAI Shuang ZHANG Mingzhen PENG Dongxiang WANG Huawei WU Maoxian CHEN Ruiling ZHANG Xiang | 2014 | Acta Geologica Sinica(English Edition)2014，88，2： | 6 |
| 17 | Effectiveness and safety of Ginkgo biloba extract(GBE50)in the treatment of dizziness caused by cerebral arteriosclerosis:a multi-center,double-blind,randomized controlled trial显示文摘OBJECTIVE:To evaluate the effectiveness and safety of Ginkgo biloba extract(GBE50)in the treatment of dizziness caused by cerebral arteriosclerosis.METHODS:This was a multi-center,double-blind,double-dummy,positive-controlled,parallel randomized controlled clinical trial with 1∶1 allocation.We recruited 404 patients with dizziness caused by cerebral arteriosclerosis(blood stasis symptom pattern)in 10 hospitals in China.GBE50 group received GBE50 and Naoxinqing tablet(NXQ)of mimetic agent,control group received NXQ and GBE50 of mimetic agent.The main outcome was Traditional Chinese Medicine(TCM)symptom pattern score of blood stasis after 6 weeks.The secondary outcomes were changes in the dizziness handicap inventory(DHI)score,vertigo visual analogue scale(VAS)score,the university of California vertigo questionnaire(UCLA-DQ)score and single-item symptom score of TCM from baseline to 2,4 and 6 weeks.Safety indicators included the incidence of adverse events,severe adverse events and laboratory examination including blood routine,liver function,renal function,and so forth.RESULTS:The total effective rate of TCM symptom pattern score in the GBE50 group after 6 weeks of treatment was higher than that in the control group,the difference in rate was statistically significant(92.67%vs 83.07%,P=0.004).Compared with the control group,there was no difference in the incidence of adverse reactions(9.95%vs 14.85%,P=0.136).CONCLUSION:The treatment of dizziness caused by cerebral arteriosclerosis with GBE50 is effective,safe and reliable. | SHA Rina TANG Lu DU Yawei WU Shengxian SHI Huawei ZOU Hongxin ZHANG Xuran DONG Xinglu ZHOU Li | 2022 | Journal of Traditional Chinese Medicine2022，42，1： | 5 |
| 18 | Bioinspired Unidirectional Liquid Transport Micro-nano Structures:A Review显示文摘Unidirectional liquid transport without any need of external energy has drawn worldwide attention for its potential applications in various fields such as microfluidics,biomedicine and mechanical engineering.In nature,numerous creatures have evolved such extraordinary unidirectional liquid transport ability such as spider sik,Sarracenia's trichomes,and Nepenthes alata's peristome,etc.This review summarizes the current progresses of natural unidirectional liquid transport on 1-Dimensional(1D)linear structure and 2-Dimensional(2D)surface stucture.The driving force of unidirectional liquid transport which is determined by unique structure exist distinct differences in physics.The fundamental understanding of 1D and 2D unidirectionaliquid transport especially about hierarchical structural characteristics and their transport mechanism were concentrated,and various bioinspired fabrication methods are also introduced.The applications of bioinspired directional liquid transport are demonstrated especially in fields of microfluidies,biomedical devices and anti-icing surfaces.With newly developed smart materials,various liquid transport regulation strategies are also summarized for the control of transport speed,direction guiding,etc.Finally,we provide new insights and future perspectives of the directional transport materials. | Liwen Zhang Guang Liu Huawei Chen Xiaolin Liu Tong Ran Yi Zhang Yang Gan Deyuan Zhang | 2021 | Journal of Bionic Engineering2021，18，1： | 4 |
| 19 | Efficient genetic transformation and CRISPR/Cas9-mediated genome editing of watermelon assisted by genes encoding developmental regulators显示文摘Cucurbitaceae is an important family of flowering plants containing multiple species of important food plants,such as melons,cucumbers,squashes,and pumpkins.However,a highly efficient genetic transformation system has not been established for most of these species(Nanasato and Tabei,2020).Watermelon(Citrullus lanatus),an economically important and globally cultivated fruit crop,is a model species for fruit quality research due to its rich diversity of fruit size,shape,flavor,aroma,texture. | Wenbo PAN Zhentao CHENG Zhiguo HAN Hong YANG Wanggen ZHANG Huawei ZHANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，4： | 4 |
| 20 | 食品废物两相高温半干法厌氧发酵生产沼气初步研究显示文摘为了提高两相厌氧发酵工艺的生产性能,该文研究了一种新的两相半干法厌氧发酵沼气生产系统,即在水解相中批式发酵,每次从工作容积为4L的产烷相中取500mL接种液,与食品废物混合后发酵1~2d,再全部输入产烷相继续生产沼气,如此循环。实验运行温度为50℃,食品废物总固体TS和挥发性固体VS分别为19.3%和16.1%,产烷相先在4gVS·d-1的进料率下单相运行至稳定,稳定期日产气量为(4.09±0.03)L(甲烷占69.0%),pH为7.50±0.02,TS和VS分别为(1.05±0.02)%和(0.64±0.02)%。稳定的产烷相与水解相联合后,水解相在60~700g的进料量下都能正常产气,但氢气含量较低。产烷相在进料量600g时产气量最高,为29.1L/d,随后受到抑制,受抑制前的甲烷含量为72.4%~74.0%,TS和VS含量也在该进料量后达到最高峰,分别为3.50%和2.32%。研究结果表明新的两相半干法厌氧发酵沼气生产系统有效提高了生产性能,在今后的研究中,需要降低从产烷相输入水解相的接种液量,以更好抑制水解相产烷微生物,提高产氢性能,还可使产烷相更加稳定,承受更高的进料量。 | 泮进明 张瑜 Zhang Ruihong 朱保宁 Sun Huawei 应义斌 | 2008 | 农业工程学报2008，24，7： | 4 |
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