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| 1 | Analysis of Aroma Volatile Compounds in Fuji Apple Using SPME with Different Fiber Coatings显示文摘The aroma volatile compounds in Fuji apple were isolated by solid-phase microextraction( SPME),and detected by gas chromatography-mass spectrometry( GC-MS). The results showed that the optimal retention time of the heating up of capillary columns was 5 min. Of the seven fibers used in this work,DVB/CAR/PDMS was found to be the most suitable to extract the aroma volatile compounds in Fuji apple. A total number of 43 volatile compounds were identified according to their retention time on capillary columns and their mass spectra,including eleven esters,ten alcohols,ten aldehydes,seven alkenes,two anhydrides,one ketone,one phenol and one ether. This detection method will provide a new foundation for analysis of volatile compounds in apple were identified. | Jie LI Jicheng HAN Haie ZHANG Chaohong ZHANG Minmin LI Yu WANG | 2020 | Agricultural Biotechnology2020，9，3： | 4 |
| 2 | Application of artificial intelligence in tongue diagnosis of traditional Chinese medicine:A review显示文摘Tongue diagnosis is an important process to non-invasively assess the condition of a patient’s internal organs in traditional Chinese medicine(TCM)and each part of the tongue is related to corresponding internal organs.Due to continuing computer technological advances,especially the artificial intelligence(AI)methods have achieved significant success in tackling tongue image acquisition,processing,and classification,novel AI methods are being introduced in traditional Chinese medicine tongue diagnosis medical practices.Traditional tongue diagnose depends on observations of tongue characteristics,such as color,shape,texture,moisture,etc.by traditional Chinese medicine physicians.The appearance of the tongue color,texture and coating reflects the improvement or deterioration of patient’s conditions.Moreover,AI can now distinguish patient’s condition through tongue images,texture or coating,which is all possible increasingly with help from traditional Chinese medicine physicians under the traditional Chinese medicine tongue theory.AI has enabled humans to do what was previously unimagined:traditional Chinese medicine tongue diagnosis with feeding a large amount of tongue image and tongue texture/coating data to train the AI modes.This review focuses on the research advances of AI in TCM tongue diagnosis thus far to identify the major scientific methods and prospects.In this article,we tried to review the AI application in resolving the tongue diagnosis of traditional Chinese medicine on color correction,tongue image extraction,tongue texture/coating segmentation. | Zhao Chen Xiaoyu Zhang Ruijin Qiu Yang Sun Rui Zheng Haie Pan Yin Jiang Changming Zhong Chen Zhao Guihua Tian Hongcai Shang | 2021 | TMR Modern Herbal Medicine2021，4，2： | 2 |
| 3 | Integrative transcriptomic and metabolomic analyses reveal the flavonoid biosynthesis of Pyrus hopeiensis flowers under cold stress显示文摘Low temperature is among the most restrictive factors to limit the yield and distribution of pear. Pyrus hopeiensis is a valuable wild resource.PCA showed that P. hopeiensis had strong cold resistance. In this study, the mRNA and metabolome sequencing of P. hopeiensis flower organs exposed to different low temperatures were performed to identify changes of genes and metabolites in response to low-temperature stress. A total of 4 851 differentially expressed genes(DEGs) were identified. Trend analysis showed that these DEGs were significantly enriched in profiles 19, 18, 7, 14, 1, 4 and 11. And the KEGG enrichment analysis showed that the DEGs in profile 18 were significantly enriched in flavone and flavonol biosynthesis. Besides, the expressed trends as well as GO and KEGG functional enrichment analyses of DEGs under cold and freezing stress showed significantly difference. Analyses of flavonoid-related pathways indicated that flavonoid structural genes had undergone significant changes. Correlation analysis showed that b HLH and MYB TFs may affect flavonoid biosynthesis by regulating structural gene expression. And PhMYB308 and PhMYB330 were likely candidate repressors of flavonoid biosynthesis by binding to a specific site in bHLH proteins. In total, 92 differentially accumulated metabolites(DAMs) were identified in P. hopeiensis flowers including 12 flavonoids. WGCNA results showed that coral 1, pink and brown 4 modules were closely associated with flavonoids and 11 MYBs and 15 bHLHs among the three modules may activate or inhibit the expression of 23 structural genes of flavonoid biosynthesis. Taken together, the results of this study provided a theoretical basis for further exploration of the molecular mechanisms of flavonoid biosynthesis and cold resistance of P. hopeiensis flower organs and our findings laid a foundation for further molecular breeding in cold-resistant pear varieties. | Yongtan Li Jun Zhang Shijie Wang Haie Zhang Yichao Liu Minsheng Yang | 2023 | Horticultural Plant Journal2023，9，3： | 1 |
| 4 | Application of adaptive noise cancellation with neural-network-based fuzzy inference system for visual evoked potentials estimation显示文摘 | YIN Haie ZENG Yanjun ZHANG Jianyhua | 2004 | Medical Engineering & Physics2004，26，1： | 1 |
| 5 | Effects of Different Sample Parts and Sterilization Methods on Contamination and Survival Rates of Shoots with Buds in Chinese Chestnut显示文摘In this study,we investigated the preferable explant types and sterilization method for tissue culture in Chinese chestnut,in order to provide a technical support for the asexual reproduction of Chinese chestnut.The base,middle and apex parts of annual shoots with buds in Chinese chestnut were sampled and cut to 2-3 cm stem segments each with one bud,then sterilized orderly with different duration in 2%NaClO plus 0.1%HgCl_(2)solutions.The results indicated that the duration of 2%NaClO for 20 min+0.1%HgCl_(2)for 15 min exerted an effective disinfection property on the middle parts of annual shoots with buds,and achieved a contamination rate under 5%and a survival rate over 90%. | Jingjing DU Meng WANG Jing LIU Xuan WANG Haie ZHANG Jingzheng ZHANG | 2023 | Agricultural Biotechnology2023，12，2： | 0 |
| 6 | Construction of Technology System on Development and Repropagation in Vitro of Several Cultivars in Pear显示文摘It is the most available way to obtain self-rooted seedlings by tissue culture,which could breed de-virus seedlings with uniform traits and contribute to improve seedling quality.To construct a complete and efficient technology system,this test used stem with buds of 16 cultivars and super lines including Yali,Jingbaili,03-04-034,03-08-080 and so on as materials to study the best way for explant sampling time and position,sterilization time using HgCl 2 and hormone concentrations.The result indicated that the optimum technology system was that the middle parts of longest shoots before 20 d after full-blossom were sterilized for 8 min by 0.1%HgCl 2 and inoculated on the development medium MS+6-BA 0.5 mg/L+IBA 0.2 mg/L+saccharose 3%+agar 0.6%(pH=5.8),and then trans-inoculated into propagation medium MS+6-BA 1.0 mg/L+IBA 0.3 mg/L+saccharose 3%+agar 0.6%(pH=5.8). | Yu WANG Baofeng HAO Jicheng HAN Lijuan GAO Longfei LI Shujun ZHAO Jintao XU Minghui JI Jie LI Haie ZHANG | 2020 | Agricultural Biotechnology2020，9，4： | 0 |
| 7 | Review on Fertilization Techniques of Chinese Chestnut(Castanea mollissima Blume.)显示文摘In this paper,we summed up the research advance in fertilization techniques of Chinese chestnut(Castanea mollissima Blume.),discussed the difference of fertilizer characteristics,fertilizer types,fertilization period,fertilization methods and research progress of formula fertilization in chestnut orchard,and finally prospected the future research of agricultural technical measure of chestnut fertilization work. | Jingjing DU Jing LIU Xuan WANG Zhiwei ZHANG Haie ZHANG Jingzheng ZHANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
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