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| 1 | Pediococcus Acidilactici Inhibit Biofilm Formation of Food-Borne Pathogens on Abiotic Surfaces显示文摘In this study,we aimed to examine the inhibitory effect of PA003,a Pediococcus acidilactici that produces lactic acid and antimicrobial peptides pediocin,on pathogenic biofilm formation on abiotic surfaces.PA003 and pathogens(Escherichia coli,Salmonella enterica serovar Typhimurium,Staphylococcus aureus and Listeria monocytogenes) were used to evaluate auto-aggregation,hydrophobicity,biofilm formation and biofilm formation inhibition on stainless steel,polyvinyl chloride and glass slides in terms of exclusion,displacement and competition.The results showed the highest auto-aggregation abilities were observed for one of the E.coli strains EAggEC(E58595) and the highest hydrophobic strain was observed with EPEC(E2348/69)(51.9%).The numbers of biofilm cells of E.coli,S.Typhimurium,S.aureus and L.monocytogenes on stainless steel,polyvinyl chloride and glass slide coupons were effectively reduced by approximately 4log CFU/coupon.These results demonstrate that lactic acid bacteria can be used as an alternative to effectively control the formation of biofilms by food-borne pathogens. | Xiqian Tan Ye Han Huazhi Xiao Zhijiang Zhou | 2017 | Transactions of Tianjin University2017，23，1： | 3 |
| 2 | Structural Characterization of Glucan Produced by Lactobacillus sake L-7 from Sausage显示文摘A linear glucan was produced by Lactobacillus sake L-7 isolated from homemade sausage. Cultivation of the strain in Man–Rogosa–Sharpe(MRS) medium containing 50 g/L sucrose yielded 5.3 g/L of purified exopolysaccharide(EPS). The EPS was characterized by gas chromatography(GC), Fourier-transform infrared(FT-IR) spectroscopy, high-performance sizeexclusion chromatography(HPSEC), nuclear magnetic resonance(NMR) spectroscopy, and scanning electron microscopy(SEM). The monosaccharide composition of the EPS was glucose, and its molecular weight was 1 × 10~7 Da. The FT-IR and NMR spectra revealed that the L-7 EPS was a linear glucan with α-(1 → 6) glucosidic bonds. SEM images of the dried EPS revealed a hollow tubular structure. The water solubility index and water holding capacity of L-7 EPS were 96 and 272%, respectively. The results of hydrolysis indicated that L-7 EPS was not susceptible to hydrolysis by physiological barriers and can be used as a soluble dietary fiber with health benefits. All these characteristics suggest that L-7 EPS might have potential applications in the food, cosmetic, and pharmaceutical industries. | Fang Feng Qingqing Zhou Yanfang Yang Fangkun Zhao Renpeng Du Ye Han Zhijiang Zhou | 2019 | Transactions of Tianjin University2019，25，1： | 3 |
| 3 | The diagnostic value of computed tomography histogram analysis in thyroid malignant solitary nodules showing coarse calcifications显示文摘 | Zhang Lexing Wei Peiying Han Zhijiang Xiang Jingjing Ding Jinwang Luo Dingcun Li Mingkui | 2018 | 中华内分泌外科杂志2018，12，4： | 2 |
| 4 | Isolation, Purification, and Characterization of Exopolysaccharide Produced by Leuconostoc Citreum N21 from Dried Milk Cake显示文摘A strain with high production of exopolysaccharide(EPS) was isolated from dried milk cake(a traditional fermented food from Inner Mongolia). The strain was called N21 and later identified as Leuconostoc citreum( Leu. citreum). The strain was cultured in Man-Rogosa-Sharpe medium containing 50 g/L of sucrose for 48 h at 30 °C and the EPS purified, with a yield of 24.5 g/L. An average molecular weight of 6.07 × 10~6 g/mol was determined by high-performance size-exclusion chromatography. The structure of the purified EPS was investigated through gas chromatography, ~1H and ^(13)C nuclear magnetic resonance spectroscopy, and Fourier transform infrared spectroscopy. The results demonstrated a polysaccharide composed of D-glucopyranose units in a linear chain with consecutive α(1 → 6) linkages. No branching was found in the structure of the exopolysaccharide. The purified EPS showed high water solubility and emulsibility. Based on the thermogravimetric curve, the degradation temperature of the EPS was 308.47 °C, which suggested that the dextran in the study exhibited high thermal stability. The results indicated that Leu. citreum N21 could be widely used to produce linear EPS and that the EPS has potential applications in food science and processing as a food additive. | Yanfang Yang Fang Feng Qingqing Zhou Fangkun Zhao Renpeng Du Zhijiang Zhou Ye Han | 2019 | Transactions of Tianjin University2019，25，2： | 2 |
| 5 | Lactic acid bacteria in traditional fermented Chinese foods显示文摘 | LIU Shanna HAN Ye ZHOU Zhijiang | 2011 | Food Research International2011，44，3： | 1 |
| 6 | Isolation and identification of antifungal peptides from Bacillus BH072, a novel bacterium isolated from honey 显示文摘 | Zhao Xin Zhou Zhijiang Han Ye | 2013 | Microbiol Res2013，168，9： | 1 |
| 7 | Fecal Microbiota Succession of Piglets from Birth to Post-weaning by 454 Pyrosequencing Analysis显示文摘The objective of this study was to describe the fecal microbiota succession of piglets from birth to postweaning by pyrosequencing.The dominant bacterial phyla were Bacteroidetes,Firmicutes,Proteobacteria,and Fusobacteria,although the composition of the fecal microbiota changed as the piglets grew.Bacteroides,Parabacteroides,Prevotella,Lactobacillus,unclassified Lachnospiraceae,Ruminococcus,Oscillospira,Phascolarctobacterium,and Desulfovibrio were the core genera in the feces of piglets and existed from birth to post-weaning.Bacteroides was the most abundant at birth; however,the proportion of Bacteroides decreased as the piglets aged,while the proportion of Prevotella increased until it became the most abundant genus at the post-weaning stage.Principal coordinate analysis indicated that the microbial communities of piglet fecal samples from birth to postweaning could be separated into four groups according to the similarity of community composition.These corresponded to time points on day 0 (birth); day 7; days 14,21,and 28 (pre-weaning); and day 35 (post-weaning).This study suggests that the development of the intestinal microbiota in the piglet is a gradual and sequential process.This methodology establishes a basis for broader studies to investigate the microbiota of the piglet intestinal tract. | Jin Wang Ye Han Fanrui Meng Jinzhao Zhao Zhijiang Zhou Huan Fan | 2017 | Transactions of Tianjin University2017，23，3： | 1 |
| 8 | Green Fluorescent Protein Recombinant Nisin as a Probe for Detection of Gram-Positive Bacteria显示文摘A great amount of foodborne pathogens were Gram-positive(G+) bacteria, a threat to public health. In this study, considering the binding ability of nisin towards G+ bacteria and the stable fluorescent ability of EGFP protein, a fluorescent nisin–EGFP protein probe was constructed by a gene engineering method. Nisin and EGFP were used as the receptor and fluorophore, respectively, to detect G+ bacteria. The nisin and egfp gene were amplified separately according to the sequence published in Gen Bank using unique primers. The two genes were cloned into a pET-28b(+) vector resulting in apET-28b(+)–nisin–egfp vector. The vector was transferred into Escherichia coli(E. coli) BL21(DE3) for expression. The expressed protein was extracted, purified by a Ni–NTA column, and then tested by the SDS-PAGE method to confirm its molecular weight. Listeria monocytogenes(L.monocytogenes), Staphylococcus aureus(S. aureus), and Micrococcus luteus(M. luteus) were used as the representations of G+ bacteria. E. coli O157, representing the gram-negative(G-) bacteria, was used as a negative control. The binding specificity of the recombinant protein was performed on two types of bacteria and then detected through fluorescent microscopy. The results indicated that the nisin–EGFP probe could detect G+ bacteria at 10~8CFU/mL. | Xiqian Tan Ye Han Huazhi Xiao Zhijiang Zhou | 2017 | Transactions of Tianjin University2017，23，4： | 1 |
| 9 | Lactic acid bacteria in traditional fermented Chinese foods显示文摘 | LIU Shanna HAN Ye ZHOU Zhijiang | 2011 | Food Research International2011，44，： | 1 |
| 10 | Lactic acid bacteria in tradi- tional fermented Chinese foods显示文摘 | LIU Shanna HAN Ye ZHOU Zhijiang | 2011 | Food Research International2011，44，3： | 1 |
| 11 | Lactic acid bacteria in traditional fermented Chinese foods显示文摘 | Liu Shanna Han Ye Zhou Zhijiang | 2011 | Food Research In- ternational2011，44，3： | 1 |
| 12 | Lactic acid bacteria in traditional fermented Chinese foods显示文摘 | LIU Shanna HAN Ye ZHOU Zhijiang | 2011 | Food Research International2011，44，3： | 1 |
| 13 | Isolation, identification and primary application of bacteria from putrid alkaline silica sol显示文摘碱的硅石大音阶的第五音的腐烂在这份报纸被调查。在三件碱的硅石大音阶的第五音样品的全部的殖民地数字是 1.47 ? , | Lijie REN Ye HAN Shuwen YANG Xiqian TAN Jin WANG Xin ZHAO Jie FAN Ting DONG Zhijiang ZHOU | 2014 | Frontiers of Chemical Science and Engineering2014，8，3： | 0 |
| 14 | Differential diagnostic value of CT scan minimum attenuation values in adrenal adenomas and non-adenomas显示文摘 | Wu Ying Zhu Miaoping Han Zhijiang Wei Peiying Lei Zhikai | 2017 | 中华内分泌外科杂志2017，11，3： | 0 |
| 15 | Analytical modeling and vibration analysis of fiber reinforced composite hexagon honeycomb sandwich cylindrical-spherical combined shells显示文摘This study analyzes and predicts the vibration characteristics of fiberreinforced composite sandwich(FRCS)cylindrical-spherical(CS)combined shells with hexagon honeycomb core(HHC)for the first time based on an analytical model developed,which makes good use of the advantage of the first-order shear deformation theory(FSDT),the multi-segment decomposition technique,the virtual spring technology,the Jacobi-Ritz approach,and the transfer function method.The equivalent material properties of HHC are firstly determined by the modified Gibson’s formula,and the related energy equations are derived for the HHC-FRCS-CS combined shells,from which the fundamental frequencies,the mode shapes,and the forced vibration responses are solved.The current model is verified through the discussion of convergence and comparative analysis with the associated published literature and finite element(FE)results.The effects of geometric parameters of HHC on the dynamic property of the structure are further investigated with the verified model.It reveals that the vibration suppression capability can be greatly enhanced by reducing the ratio of HHC thickness to total thickness and the ratio of wall thickness of honeycomb cell to overall radius,and by increasing the ratio of length of honeycomb cell to overall radius and honeycomb characteristic angle of HHC. | Hui LI Bocheng DONG Zhijiang GAO Jing ZHAO Haiyang ZHANG Xiangping WANG Qingkai HAN | 2022 | Applied Mathematics and Mechanics(English Edition)2022，43，9： | 0 |
| 16 | Predictive value of enhanced CT Hounsfield unit value for central lymph node metastasis in Hashimoto’s thyroiditis with papillary carcinoma显示文摘Objective To investigate the diagnostic and predictive value of enhanced CT Hounsfield unit value for central lymph node metastasis(CLNM)in patients with Hashimoto’s thyroiditis(HT)complicated with papillary thyroid carcinoma(PTC).Methods The CT images of HTs with PTC confirmed by operation and pathology from 88 patients were analyzed retrospectively.Among them,50 cases of CLNM were all negative and 38 cases of CLNM were all positive.One lymph node was selected as the study subject in each case.The average HU values(AHUVs)and maximum HU values(MHUVs)were measured on the enhanced CT.The diagnostic efficacy of the two parameters for diagnosing CLNM was analyzed by the receiver operating specificity curve(ROC),and the best cut-off values were obtained,which were used to predict 103 HT with PTC CLNM in the validation group.Results The AHUVs of negative group and positive group were(63.79±15.34)HU and(90.92±18.04)HU(t=8.828,P<0.001).The MHUVs of negative group and positive group were(77.08±15.30)HU and(108.79±18.37)HU,respectively(t=7.615,P<0.001).The AUCs for AHUVs and MHUVs for the diagnosis of CLNM-positive was 0.870 and 0.906,and the cut-off values were 84.0 HU and 96.5 HU,respectively.The sensitivity,specificity,and accuracy of predicting CLNM positivity in the validation group using AHUVs≥84.0 HU were 35.7%,95.1%,and 70.9%,respectively,and MHUVs≥96.5 HU were 38.1%,93.4%,and 70.9%,respectively.Conclusion AHUVs≥84.0 HU and MHUVs≥96.5 HU have high specificity for the diagnosis of HT with PTC CLNM,which can provide an important basis for clinical treatment decision. | Tian Min Zhang Tong Shu Yanyan Wei Peiying Shao Chang Han Zhijiang | 2023 | 中华内分泌外科杂志2023，17，1： | 0 |
| 17 | Image moments-based visual servoing control of bagged agricultural materials handling robot显示文摘Manual handling is less efficient and sometimes even hazardous to humans in many areas,for example,agriculture.Using robots in those areas not only avoids human contact with such dangerous agricultural materials but also improves working efficiency.The motion of a robot is controlled using a technique called visual servoing that uses feedback information extracted from a vision sensor.In this study,a visual servoing method was proposed based on learning features and image moments for 3D targets to solve the problem of image moment-based visual servoing.A Gaussian process regression model was used to map the relationship between the image moment invariants and the rotational angles around the X-and Y-axes of the camera frame(denoted asγandβ).To obtain maximal decoupled structure and minimal nonlinearities of the image Jacobian matrix,it was assumed two image moment features,which are linearly proportional toγandβ.In addition to the other four standardized image moment features,a 6-DOF image moment-based visual servoing controller for the agricultural material handling robot was designed.Using this method,the problem of visual servoing task failure due to the singularity of the Jacobian matrix was solved,and it also had a better convergence effect for the part of the target image beyond the field of view and large displacement visual servoing system.The proposed algorithm was validated by carrying out experiments tracking bagged flour in a six-degree-of-freedom robotic system.The final displacement positioning accuracy reached the millimeter level and the direction angle positioning accuracy reached the level of 0.1°.The method still has a certain convergence effect when the target image is beyond the field of view.The experimental results have been presented to show the adequate behavior of the presented approach in robot handling operations.It provides reference for the application of visual servoing technology in the field of agricultural robots and has important theoretical significance and practical value. | Han Li Zhijiang Zuo Ruijuan Chi Yuefeng Du Enrong Mao | 2023 | International Journal of Agricultural and Biological Engineering2023，16，1： | 0 |
| 18 | Experimental Investigation on Vibration Reduction Performance of Fiber Metal Laminate Beams with MRE Core显示文摘The vibration reduction characteristics of composite beams filled with magnetorheological elastomer core are studied experimentally.The fiber metal laminates with magnetorheological elastomers core is self-designed and prepared.Internal magnetic field is applied to the beam to explore its action of damping vibration performance under the magnetic field for the first time.The composite elements test system with controllable magnetic field intensity is designed and the function of each part is introduced.Then,a set of reasonable and standard vibration test flow of this type of composite beam under different magnetic field intensity is clarified,and the practical test is conducted.It has been found that the composite beam has excellent damping performance with the first 4 damping ratios being greater than 10%.Moreover,after the magnetic field is applied,its damping results can be further improved to meet the active control purpose. | Jintong HAN Wenyu WANG Xintong WANG Xiaoyue HU Ziheng WANG Zhijiang GAO Hui LI | 2019 | Research and Application of Materials Science2019，1，2： | 0 |
| 19 | Value of CT to assess calcification patterns in thyroid nodules显示文摘 | Wei Peiying Shu Yanyan Han Zhijiang Luo Dingcun | 2017 | 中华内分泌外科杂志2017，11，4： | 0 |
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