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| 1 | Construction of a System for the Stable Expression of Foreign Genes in Dunaliella salina显示文摘A stable transformation system for the expression of foreign genes in the unicellular greenmarine alga (Dunaliella salina Teod.) was established. Using electroporation, the alga was transformed witha plasmid containing the hepatitis B surface antigen (HBsAg) gene and the chloramphenicol acetyltransferase(CAT) gene as a selectable gene. PCR and Southern blotting analysis indicated that the HBsAEgene wasintegrated into the D. salina genome. Northern dotting analysis showed that the HBsAg gene was expressedat the mRNA level. The stable expression of HBsAg protein in transformants was confirmed by HBsAgenzyme-linked immunosorbent assay (HBsAg EUSA) and Western blotting analysis. Also, PCR and Southernblotting analyses showed that the CA Tgene was integrated into the D, salina genome, and CAT EUSAindicated that CAT protein was stably expressed in the cells. The introduced HBsAg DNA and HBsAgprotein expression were stably maintained for at least 60 generations in media devoid of chloramphenicol.This is the first report of the stable expression of foreign genes in D. salina. | GENGDe-Gui HANYan WANGYi-Qin WANGPeng ZHANGLi-Ming LIWen-Bin SUNYong-Ru | 2004 | Acta Botanica Sinica2004，46，3： | 9 |
| 2 | Capacitive charge storage enables an ultrahigh cathode capacity in aluminum-graphene battery显示文摘Aluminum^-graphene battery is promising for its abundant raw materials,high power density,ultralong cycle life and superior safety.However,the development of aluminum^-graphene battery is currently restricted by its insufficient cathode capacity,calling for a newly developed working mechanism.In addition,an irregular constant increase of the cathode capacity was always observed during cycling,but cannot be explained based on the current understanding.Here,we observed an increase of specific capacity by 60%with stable Coulombic efficiency of 98%during 7000 cycles life of Al-graphene batteries employing AlCl3/ET3NHCl electrolyte.We demonstrated this growing cathode capacity is attributed to an increasing contribution of capacitive charge storage during cycling,because a gradually enlarged surface area as capacitive active sites is enabled by the exfoliation of graphitic cathode during the periodic intercalation process.Moreover,the graphene cathode was exfoliated more significantly in AlCl3/ET3NHCl than 1-ethyl-3-methylimidazolium chloride-based electrolyte,which results from the heavier stress on the graphene layers caused by the larger intercalants in AlCl3/ET3NHCl.The common intercalation of cations with AlCl4-clusters was therefore supposed to occur during charging.This new proposed mechanism can offer the new thought for future design on high-capacity cathode of Al-ion battery. | Hanyan Xu Hao Chen Haiwen Lai Zheng Li Xiaozhong Dong Shengying Cai Xingyuan Chu Chao Gao | 2020 | Journal of Energy Chemistry2020，29，6： | 4 |
| 3 | Methylglyoxal increases dopamine level and leads to oxidative stress in SH-SY5Y cells显示文摘越来越多的研究建议了 type-2 糖尿病导致的 methylglyoxal (MGO ) 与 Parkinson 的疾病(PD ) 有关。然而,很少对分子的机制被知道。在这研究,我们在 neuroblastoma SH-SY5Y 房间探索了 MGO 毒性。MGO 的 Neurotoxicity 被 mitochondrial 膜潜力, malondialdehyde,和 methylthiazoletetrazolium 试金测量。多巴胺, 3,4-dihydroxyphenylacetic 酸(DOPAC ) ,和 1-methyl-4-phenyl-1,2,3,4-tetrahydroisoquinoline (salsolinol ) 的层次被液体 chromatographymass spectrometry/mass spectrometry 检测。酷氨酸 hydroxylase (TH ) 和多巴胺 transporter (DAT ) 的表情被反向的 transcriptase 聚合酶链反应和西方的污点分析检测。当多巴胺, DOPAC,和内长的神经毒素 salsolinol 的层次也增加了时,结果证明 MGO 在 SH-SY5Y neuroblastoma 房间在 TH 和 DAT 表情导致了增加。Aminoguanidine (AG ) 是 MGO 的一个禁止者。AG 能减少,这被发现反应的氧种类(ROS ) 水平由 MGO 导致了,但是不能禁止 TH, DAT 和多巴胺的增加。多巴胺, DOPAC 和 salsolinol 层次的增加能导致高 ROS 和 mitochondrial 损坏。当 MGO 在糖尿病的功课期间被增加时,这研究建议多巴胺引起的 ROS 能贡献 dopaminergic 神经原的损坏。 | Bingjie Xie Fankai Lin Lei Peng Kaleem Ullah Hanyan Wu Hong Qing Yulin Deng | 2014 | Acta Biochimica et Biophysica Sinica2014，46，11： | 4 |
| 4 | Mineral Chemistry Indicates the Petrogenesis of Rhyolite From The Southwestern Okinawa Trough显示文摘To reveal the petrogenesis of rhyolite from the southwestern Okinawa Trough, the mineral chemistry of plagioclase, orthopyroxene, amphibole, quartz and Fe-Ti oxide phenocrysts were analyzed using an electron microprobe, and in suit Sr and Ba contents of plagioclase analysed by LA-ICPMS were chosen for fingerprinting plagioclases of different provenances. Results indicate an overall homogeneous composition for each of the mineral phases except for plagioclase phenocrysts which have a wide range of composition(An=39~88). Plagioclase crystals characterized by An contents of >70 are not in equilibrium with their whole-rock compositions, and coarse-sieved plagioclase phenocryst interiors record high An contents(>70) and Sr/Ba ratios(>7), which are similar to the those of plagioclase crystals in basalt. Therefore, these crystals must have been introduced to the rhyolitic magma from a more mafic source. Equilibrium temperatures estimated using orthopyroxene-liquid, iron–titanium oxide, titanium-in-quartz and amphibole geothermometers show consistent values ranging from 792 to 869℃. The equilibrium pressure calculated using amphibole compositions is close to 121 MPa which corresponds to an approximate depth of 4 km. The fO_2 conditions estimated from Fe-Ti oxides and amphiboles plot slightly above the NNO buffer, which indicates that the rock formed under more oxidized conditions. Our results suggests that petrogenesis of the rhyolite due to basaltic magma ascend with the high An and Sr/Ba plaigoclases from deep magma chamber into the shallow chamber where the fractional crystallization and crustal assimilation happened. It also indicates that a two-layer magma chamber structure may occur under the southwestern Okinawa Trough. | CHEN Zuxing ZENG Zhigang WANG Xiaoyuan ZHANG Yuxiang YIN Xuebo CHEN Shuai MA Yao LI Xiaohui QI Hanyan | 2017 | Journal of Ocean University of China2017，16，6： | 4 |
| 5 | Ⅰ型干扰素信号通路上的“刹车系统”:干扰素刺激基因IFIT5显示文摘天然免疫是机体抵御外源病原微生物侵入的第一道防线,而Ⅰ型干扰素(IFN-I)信号通路是天然免疫信号通路中关键的组成部分。病毒感染宿主后,由模式识别受体识别并介导产生IFN-I,IFN-I进而通过Jak-STAT信号通路诱导上百种干扰素刺激基因(ISGs)的表达。正常情况下,IFN的产生和其下游信号通路的激活受到精准的调控。 | ZHANG NA SHI HANYAN MIAO-MIAO | 2021 | 中国预防兽医学报2021，43，6： | 3 |
| 6 | Effect of lubricant sulfur on the morphology and elemental composition of diesel exhaust particles显示文摘This work investigates the effects of lubricant sulfur contents on the morphology,nanostructure,size distribution and elemental composition of diesel exhaust particle on a light-duty diesel engine. Three kinds of lubricant(LS-oil,MS-oil and HS-oil,all of which have different sulfur contents:0.182%,0.583% and 1.06%,respectively)were used in this study. The morphologies and nanostructures of exhaust particles were analyzed using high-resolution transmission electron microscopy(TEM). Size distributions of primary particles were determined through advanced image-processing software. Elemental compositions of exhaust particles were obtained through X-ray energy dispersive spectroscopy(EDS). Results show that as lubricant sulfur contents increase,the macroscopic structure of diesel exhaust particles turn from chain-like to a more complex agglomerate. The inner cores of the core-shell structure belonging to these primary particles change little; the shell thickness decreases,and the spacing of carbon layer gradually descends,and amorphous materials that attached onto outer carbon layer of primary particles increase. Size distributions of primary particles present a unimodal and normal distribution,and higher sulfur contents lead to larger size primary particles. The sulfur content in lubricants directly affects the chemical composition in the particles. The content of C(carbon)decreases as sulfur increases in the lubricants,while the contents of O(oxygen),S(sulfur)and trace elements(including S,Si(silicon),Fe(ferrum),P(phosphorus),Ca(calcium),Zn(zinc),Mg(magnesium),Cl(chlorine)and Ni(nickel))all increase in particles. | Piqiang Tan Yuan Li Hanyan Shen | 2017 | Journal of Environmental Sciences2017，29，5： | 3 |
| 7 | Study of High Frequency Folded Waveguide BWO with MEMS Technology显示文摘 | Feng Jinjun Ren Dapeng Li Hanyan | | 0，，04： | 1 |
| 8 | Analysis of molding process parameters of bra cup显示文摘 | WU Hanyan ZHANG Xin YING Boan | 2009 | Textile Bioengineering and Informatics Symposium Proceedings2009，5，6： | 1 |
| 9 | Operational optimization of air conditioning cooling water system with UF-RO desali- nation显示文摘 | HanYan Lifen Liu Fenglin Yang | 2010 | Desalination2010，251，1： | 1 |
| 10 | Study of High Frequency Folded Waveguide BWO with MEMS Technology显示文摘 | FENG Jinjun REN Dapeng LI Hanyan | | 0，，04： | 1 |
| 11 | Anal- ysis of molding process parameters of bra cup 显示文摘 | WU Hanyan ZHANG Xin YING Boan | 2009 | Textile Bioengineering and Informatics Symposium Proceedings2009，5，6： | 1 |
| 12 | An Improved Reversible DataHiding in Encrypted Images Using Side Match显示文摘 | HONGW CHEN T S Wu Hanyan | 2012 | IEEE Signal ProcessLetters2012，19，4： | 1 |
| 13 | Identification of a glycine-fich protein from the tick Rhipicephalus haemaphysa- loides and evaluation of its vaccine potential against tick feeding 显示文摘 | Zhou JinLin Gong hanyan Zhou yongzhi | 2006 | Original Paper2006，100，1： | 1 |
| 14 | Study of high frequency folded waveguide BWO with MEMS technology显示文摘 | FENG Jinjun REN Dapeng LI Hanyan | 2011 | Terahertz Science and Technology2011，4，4： | 1 |
| 15 | Identification of a glycinerich protein from the tick Rhipicephalus haemaphysaloides and evaluation of its vaccine potential against tick feeding显示文摘 | Zhou Jinlin Gong hanyan Zhou yongzhi | 2006 | Original Paper2006，，4： | 1 |
| 16 | The convergence of (DG) fuzzy integrals显示文摘 | Song Shiji Shi Penlin Wang Hanyan | 1997 | Fuzzy Sets and Systems1997，90，： | 1 |
| 17 | Study of high frequency Folded Waveguide BWO with MEMS technology显示文摘 | FENG Jinjun REN Dapeng LI Hanyan | 2011 | Terahertz Science and Technology2011，4，4： | 1 |
| 18 | Study of High Frequency Folded Waveguide BWO with MEMS Technology显示文摘 | Feng Jinjun Ren Dapeng Li Hanyan | | 0，，04： | 1 |
| 19 | Characteriza- tion of a marine origin aerobic nitrifying-denitrifying bacteri- um显示文摘 | Zheng Hanyan Liu Ying Gao Xiyan | 2012 | Journal of Bioscience and Bioengineering2012，114，1： | 1 |
| 20 | Discrimination between Chinese rice wines of differentgeographical origins by NIRS and AAS 显示文摘 | Hanyan Y Ying Z Xiaping F | 2007 | European Food Research and Technology2007，225，： | 1 |
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