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| 1 | PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome显示文摘Generation of transgenic mice by DNA microinjection has become one of the most important technologies in studying gene function in vivo. Ran- dom integration of transgene often results in inser- tional mutation which may complicate phenotype analysis of transgenic mice and/or create a good opportunity to study the function of endogenous gene. However,the utilization of these potentially valuable resources is hampered due to lack of efficient ap- proaches for rapid identification of multi-copy trans- gene integration sites. Here we propose two PCR-based approaches to identifying trans- gene/genome junction sequences. The efficiency of these two strategies was tested in 9 independent transgenic mouse lines. Among them,the transgene in 8 mouse lines was clearly localized to certain chromosome regions. These rapid and efficient ap- proaches will greatly facilitate the study of insertional mutation due to transgene integration. | ZHAO Xudong DANG Suying LIANG Bin LEI Xia CHEN Zheng WANG Long YAN Lanzhen SUN Hantang FU Jiliang FEI Jian WANG Zhugang | 2006 | Chinese Science Bulletin2006，51，18： | 2 |
| 2 | Growth of α-HgI2 single crystals from physical vapor trans- port in an oil-bath furnace显示文摘 | Zhou Hantang Lee Chenghsu Chung Jiaming | 2003 | Mater Res Bull2003，38，15： | 1 |
| 3 | Growth of a-Hglz Single Crystals from Physical Vapor Transport in an Oil-bath Furnace显示文摘 | Zhou HanTang Lee ChengHsu Chung JiaMing | 2003 | Materi- als Research Bulletin2003，38，： | 1 |
| 4 | Growth of a-HgI2 Single Crystals from Physical Vapor Transport in an Oil-Bath Furnace 显示文摘 | Zhou Hantang Lee Chenghsu Chung Jiaming | 2003 | Materials Research Bulletin2003，38，15： | 1 |
| 5 | Detection of vertical root fractures in non‐endodontically treated molars using cone‐beam computed tomography: a report of four representative cases显示文摘 | Ping Wang Wenxi He Hantang Sun Qun Lu Longxing Ni | 2011 | Dental Traumatology2011，，： | 1 |
| 6 | Effect of gypsum on strength of Portland cement with large amount of slag显示文摘 | YANG Shuzhen SONG Hantang MOU Shanbin | 1995 | J Wuhan Univ Tech (in Chinese)1995，17，1： | 1 |
| 7 | Advances in sequencing and key character analysis of mango (Mangifera indica L.)显示文摘Mango(Mangifera indica L.)is an important fruit crop in tropical and subtropical countries associated with many agronomic and horticultural problems,such as susceptibility to pathogens,including powdery mildew and anthracnose,poor yield and quality,and short shelf life.Conventional breeding techniques exhibit significant limitations in improving mango quality due to the characteristics of long ripening,self-incompatibility,and high genetic heterozygosity.In recent years,much emphasis has been placed on identification of key genes controlling a certain trait through genomic association analysis and directly breeding new varieties through transgene or genotype selection of offspring.This paper reviews the latest research progress on the genome and transcriptome sequencing of mango fruit.The rapid development of genome sequencing and bioinformatics provides effective strategies for identifying,labeling,cloning,and manipulating many genes related to economically important traits.Preliminary verification of the functions of mango genes has been conducted,including genes related to flowering regulation,fruit development,and polyphenol biosynthesis.Importantly,modern biotechnology can refine existing mango varieties to meet the market demand with high economic benefits. | Miaoyu Song Haomiao Wang Zhiyi Fan Hantang Huang Huiqin Ma | 2023 | Horticulture Research2023，10，2： | 0 |
| 8 | Hexyl substitution of pentathienoacene toward a significant improvement in charge transport显示文摘A novel semiconductor, dihexyl-substituted pentathienoacene(C6-PTA) is designed and synthesized in five steps with the total yield up to 25.2%. The introduction of hexyl chains endow the thin film semiconductor with about threefold increase in carrier mobility and one to three orders of magnitude improvement in current on/off ratio. Furthermore, single crystal FETs based on C6-PTA exhibited mobility up to 0.64 cm^2 V^(-1) s^(-1), which is over 50 times higher than the thin film counterpart. The results indicate clearly that C6-PTA is a promising organic semiconductor with high stability. | Ping He Hantang Zhang Chunhui Xu Yonggang Zhen Huanli Dong Wenping Hu | 2019 | Chinese Chemical Letters2019，30，4： | 0 |
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