2篇
您的检索式：作者名="Haoyan Meng"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo显示文摘Coxsackievirus belongs to the Picornaviridae family and is one of the major pathogens that cause hand,foot and mouth disease(HFMD)in infants and children with potential serious complications and even deaths.The pathogenesis of this virus is not fully elucidated and no vaccine or antiviral drug has been approved.In this study,a full-length infectious cDNA clone of coxsackievirus B5 virus was assembled and the recombinant virus displayed similar growth kinetics and ability to cause cytopathic effects as the parental virus.Luciferase reporter was then incorporated to generate both full-length and subgenomic replicon(SGR)reporter viruses.The full-length reporter virus is suitable for high-throughput antiviral screening,while the SGR is a useful tool to study viral-host interactions.More importantly,the full-length reporter virus has also been shown to infect the suckling mouse model and the reporter gene could be detected using an in vivo imaging system,thus providing a powerful tool to track viruses in vivo.In summary,we have generated coxsackievirus B5 reporter viruses and provided unique tools for studying virus-host interactions in vitro and in vivo as well as for high-throughput screenings(HTS)to identify novel antivirals. | Shangrui Guo Meng Xun Tingting Fan Xinyu Li Haoyan Yao Xiaozhen Li Bo Wu Hang Yang Chaofeng Ma Hongliang Wang | 2023 | Virologica Sinica2023，38，4： | 0 |
| 2 | Performance of feeding Artemia with bioflocs derived from two types of fish solid waste显示文摘The production of bioflocs with the solid waste from recirculating aquaculture systems(RAS)and feeding Artemia results in additional nutrient retention and lowers waste discharged from RAS.The solid waste from the drumfilters of two RAS,which stocked European eel(Anguilla anguilla)and Nile tilapia(Oreochromis niloticus),was used as substrate to produce bioflocs in suspended growth reactors,referred to as E-flocs and T-flocs,respectively.Mono-diets consisting of 100%E-flocs and 100%T-flocs were added to culture Artemia,referred as E-Artemia and T-Artemia,respectively,in a laboratory scale test.The efficiency of this feeding regime was investigated.A significant difference was observed in terms of crude protein content(35.59±0.2%)for E-flocs,(29.29±0.95)%for T-flocs,(70.01±0.92)%for E-Artemia and(65.63±0.89)%for T-Artemia.134 out of the total operational taxonomic units(OTUs)were present in E-flocs and T-flocs from the analysis of high-throughput sequencing data.Most of the shared OTUs belonged to cyanobacteria.C18:1n7 of T-flocs was higher than that of E-flocs(P<0.05).C18:2n6 of E-flocs was significantly higher than that of T-flocs(P<0.05).No significant difference was observed in the other fatty acid compositions(P>0.05).The survival rate of E-Artemia was(22±0.02)%,significantly higher than that of T-Artemia(16%±0.02%)(P<0.05).No significant difference was observed between the average body weight of E-Artemia(2.38±0.40 mg)and E-Artemia(2.91±0.21)(P>0.05).The EPA of Artemia fed with E-flocs was(3.00±0.46)%,significantly higher than that of T-Artemia(1.57±0.19%)(P<0.05).This study offers a method for reusing the aquaculture waste,which will be helpful to achieve a zero-pollution discharge for aquaculture systems. | Miaolan Yao Guozhi Luo Hongxin Tan Lipeng Fan Haoyan Meng | 2018 | Aquaculture and Fisheries2018，3，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员