|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence显示文摘Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of 'Darmor-bzh'and 'Tapidor,'respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding. | Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang | 2019 | Molecular Plant2019，12，1： | 10 |
| 2 | Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors显示文摘Programmed cell death protein-1(PD-1)-mediated immunosuppression has been proposed to contribute to the limited clinical efficacy of chimeric antigen receptor T(CAR-T)cells in solid tumors.We generated PD-1 and T cell receptor(TCR)deficient mesothelin-specific CAR-T(MPTK-CAR-T)cells using CRISPR-Cas9 technology and evaluated them in a dose-escalation study.A total of 15 patients received one or more infusions of MPTK-CAR-T cells without prior lymphodepletion.No dose-limiting toxicity or unexpected adverse events were observed in any of the 15 patients.The best overall response was stable disease(2/15 patients).Circulating MPTK-CAR-T cells peaked at days 7–14 and became undetectable beyond 1 month.TCR-positive CAR-T cells rather than TCR-negative CAR-T cells were predominantly detected in effusion or peripheral blood from three patients after infusion.We further confirmed the reduced persistence of TCR-deficient CAR-T cells in animal models.Our results establish the preliminary feasibility and safety of CRISPR-engineered CAR-T cells with PD-1 disruption and suggest that the natural TCR plays an important role in the persistence of CAR-T cells when treating solid tumors. | Zhenguang Wang Na Li Kaichao Feng Meixia Chen Yan Zhang Yang Liu Qingming Yang Jing Nie Na Tang Xingying Zhang Chen Cheng Lianjun Shen Jiaping He Xun Ye Wei Cao Haoyi Wang Weidong Han | 2021 | Cellular & Molecular Immunology2021，18，9： | 7 |
| 3 | In vitro transcribed sgRNA causes cell death by inducing interferon release显示文摘Dear editor The clustered regularly in terspaced short palindromic repeats (CRISPR) and the CRISPR-associated proteins 9 (Cas9) systems are powerful tools for gene editing. Ribonucleoprotein (RNP) complex composed of Cas9 pro? tein and sgRNA binds to specific genomic loci and gen erate DNA double strand breaks. While plasmids expressing Cas9 protein and sgRNA are routinely transfected into various cell lines to perform gene editing (Cong et al., 2013;Mali et al., 2013;Ran et al., 2013), direct delivery of Cas9-sgRNA RNP has shown higher efficiency and lower off-target effects (Kim et al., 2014), especially in human primary cells such as T cells (Hendel et al., 2015;Schumann et al., 2015). SgRNA can be gen erated by either in vitro transcript! on (IVT) or chemical syn thesis. IVT is widely used to gen erate sgRNAs, since it can be easily performed in most labs. | Wei Mu Na Tang Chen Cheng Wen Sun Xiaofei Wei Haoyi Wang | 2019 | Protein & Cell2019，10，6： | 2 |
| 4 | Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip显示文摘The identification of tumor-related microRNAs(miRNAs)exhibits excellent promise for the early diagnosis of cancer and other bioanalytical applications.Therefore,we developed a sensitive and efficient biosensor using polyadenine(polyA)-mediated fluorescent spherical nucleic acid(FSNA)for miRNA analysis based on strand displacement reactions on gold nanoparticle(AuNP)surfaces and electrokinetic signal amplification(ESA)on a microfluidic chip.In this FSNA,polyA-DNA biosensor was anchored on AuNP surfaces via intrinsic affinity between adenine and Au.The upright conformational polyA-DNA recognition block hybridized with 6-carboxyfluorescein-labeled reporter-DNA,resulting in fluorescence quenching of FSNA probes induced by AuNP-based resonance energy transfer.Reporter DNA was replaced in the presence of target miRNA,leading to the recovery of reporter-DNA fluorescence.Subsequently,reporter-DNAs were accumulated and detected in the front of with Nafion membrane in the microchannel by ESA.Our method showed high selectivity and sensitivity with a limit of detection of 1.3 pM.This method could also be used to detect miRNA-21 in human serum and urine samples,with recoveries of 104.0%-113.3% and 104.9%-108.0%,respectively.Furthermore,we constructed a chip with three parallel channels for the simultaneous detection of multiple tumor-related miRNAs(miRNA-21,miRNA-141,and miRNA-375),which increased the detection efficiency.Our universal method can be applied to other DNA/RNA analyses by altering recognition sequences. | Jun Xu Qing Tang Runhui Zhang Haoyi Chen Bee Luan Khoo Xinguo Zhang Yue Chen Hong Yan Jincheng Li Huaze Shao Lihong Liu | 2022 | Journal of Pharmaceutical Analysis2022，12，5： | 0 |
| 5 | Mobile Sensing in the COVID-19 Era:A Review显示文摘Background. During the COVID-19 pandemic, mobile sensing and data analytics techniques have demonstrated their capabilitiesin monitoring the trajectories of the pandemic, by collecting behavioral, physiological, and mobility data on individual,neighborhood, city, and national scales. Notably, mobile sensing has become a promising way to detect individuals’ infectiousstatus, track the change in long-term health, trace the epidemics in communities, and monitor the evolution of viruses andsubspecies. Methods. We followed the PRISMA practice and reviewed 60 eligible papers on mobile sensing for monitoringCOVID-19. We proposed a taxonomy system to summarize literature by the time duration and population scale under mobilesensing studies. Results. We found that existing literature can be naturally grouped in four clusters, including remote detection,long-term tracking, contact tracing, and epidemiological study. We summarized each group and analyzed representative workswith regard to the system design, health outcomes, and limitations on techniques and societal factors. We further discussed theimplications and future directions of mobile sensing in communicable diseases from the perspectives of technology andapplications. Conclusion. Mobile sensing techniques are effective, efficient, and flexible to surveil COVID-19 in scales of timeand populations. In the post-COVID era, technical and societal issues in mobile sensing are expected to be addressed toimprove healthcare and social outcomes. | Zhiyuan Wang Haoyi Xiong Mingyue Tang Mehdi Boukhechba Tabor E.Flickinger Laura E.Barnes | 2022 | Health Data Science2022，，1： | 0 |
| 6 | Heterogeneous catalytic dehydrogenative coupling of ethylene glycol and primary alcohols intoα-hydroxycarboxylic acids显示文摘Lactic acid and otherα-hydroxycarboxylic acids(α-HCAs)play crucial roles in various applications.Synthesizingα-HCAs from biomass platform feedstocks such as ethylene glycol(EG)and primary alcohols is novel and attractive.It was reported that the dehydrogenative cross-coupling of EG and primary alcohols can be achieved via homogeneous catalysis.Herein,we report a heterogeneous catalytic strategy to produce a series ofα-HCAs through the same reaction pathway.Impressive catalytic activity and selectivity were achieved using various metals(Ru,Ir,Pt and Pd)supported on the nanodiamond-graphene(ND@G),with Ru exhibiting the best performance.This universally applicable process enables the easy synthesis of gram-scaleα-HCAs,providing a straightforward and compelling C–C bond cross-coupling strategy for the utilization of alcohols derived from biomass feedstocks. | Shuheng Tian Jiarui Li Xingjie Peng Yao Xu Maoling Wang Haoyi Tang Wu Zhou Meng Wang Ding Ma | 2023 | Science China Chemistry2023，66，9： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员