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| 1 | Macrophage migration inhibitory factor stimulated by He/icobacter py/oriincreases proliferation of gastric epithelial cells显示文摘AIM: Helicobacter pylori (H pylori) is associated with increased gastric inflammatory and epithelial expression of macrophage migration inhibitory factor (MIF) and gastric epithelial cell proliferation. This study aimed at determining whether H pylori directly stimulates release of MIF in monocytes, whether the cay pathogenicity island (PAI) is involved for this function, and whether MIF stimulated by H pylori increases gastric epithelial cell proliferation in vitro.METHODS: A cytotoxic wild-type H pylori strain (TN2),its three isogenic mutants (TN2△cag, TN2△cagA and TN2△cagE) were co-cultured with cells of a human monocyte cell line, THP-1, for 24 h at different organism/cell ratios. MIF in the supernatants was measured by an ELISA. Cells of a human gastric cancer cell line, MKN45,were then co-cultured with the supernatants, with and without monoclonal anti-MIF antibody for 24 h. The cells were further incubated for 12 h after addition of 3H-thymidine, and the levels of incorporation of 3H-thymidine were measured with a liquid scintillation counter.RESULTS: The wild-type strain and the isogenic mutants,TN2△cagA and TN2△cagE, increased MIF release at organism/cell ratios of 200/1 and 400/1, but not at the ratios of 50/1 and 100/1. However, the mutant TN2△cag did not increase the release of MIF at any of the four ratios.3H-thymidine readings for MKN-45 cells were significantly increased with supernatants derived from the wild-type strain and the mutants TN2△cagA and TN2△cagE, but not from the mutant TN2△cag. Moreover, in the presence of monoclonal anti-MIF antibody, the stimulatory effects of the wild-type strain on cell proliferation disappeared.CONCLUSION: H pylori stimulates MIF release in monocytes, likely through its cag PAI, but not related to cagA or cagE. H pylori-stimulated monocyte culture supernatant increases gastric cell proliferation, which is blocked by anti-MIF antibody, suggesting that MIF plays an important role in H pylori-induced gastric epithelial cell proliferation. | Harry Hua-Xiang Xia Shiu Kum Lam Annie O.O.Chan Marie Chia Mi Lin Hsiang Fu Kung Keiji Ogura Douglas E.Berg Benjamin Chun-Yu Wong | 2005 | World Journal of Gastroenterology2005，11，13： | 12 |
| 2 | Isozyme variability and differentiation between Rhodnius prolixus, Rhodnius robustus and Rhodnius pictipes, vectors of Chagas' disease in Venezuela显示文摘 | Harry MI Calindez I and Cariou ML | 1992 | Med Vet Entomol1992，6，1： | 1 |
| 3 | Comparison of metabolic pathwangs bethwangs between cancer cells and slromal cells in colorectal carcinomas:a metabolic survival role for tumor associated slroma显示文摘 | Kououmkis MI Gialromanolaki A Harris AL | 2006 | Cancer Res2006，66，2： | 1 |
| 4 | Mortality among diabetesin a national sample显示文摘 | Kleinman JC Donahue RP Harris MI | 1988 | Am J Epidemiol1988，128，2： | 1 |
| 5 | Onset of NIDDM occurs at least 4-7 years before clinical diagnosis显示文摘 | Harris MI Klein R Welbom TA | 1992 | Diabetes Care1992，15，： | 1 |
| 6 | Racial and ethnic differences in glycemic control of adults with type 2 diabetes显示文摘 | Harris MI Eastman RC Cowries CC | 1999 | Diabetes Care1999，22，3： | 1 |
| 7 | The contractile properties of the elderly human diaphragm显示文摘 | Polkey MI Harris ML Hughes PD | 1997 | Am J Respir Crit Care Meal1997，155，5： | 1 |
| 8 | Onset of NIDDM occurs at least 4~7 years before clinical diagnosis显示文摘 | Harris MI Klein R Welborn TA | 1992 | Diabetes Care1992，15，7： | 1 |
| 9 | Tic exacerbation and precipitation during atomoxetine treatment in two children with attention-deficit hyperactivity disorder显示文摘 | Parraga HC Parraga MI Harris DK | 2007 | In J Psychiatry Med2007，37，4： | 1 |
| 10 | Prevalence of Diabetes,impaired fasting glucose,and impaired glucose tolerance in US adults显示文摘 | Flegal KM Cowie CC | 1999 | Diabetes Care1999，21，4： | 1 |
| 11 | Effect of lead acetate on nitrite production by murine brain endothelial cell cultures显示文摘 | BLAZKA ME HARRY GJ LUSTER MI | 1994 | Toxicol Appl Pharmacol1994，126，： | 1 |
| 12 | Comparison of metabolic pathways between cancer cells and stromal cells in colorectal carcinomas: a metabolic survival role for tumor-associated stroma显示文摘 | Koukourakis MI GA Harris AL Sivridis E | 2006 | Cancer Res2006，66，2： | 1 |
| 13 | Racial and ethnic differ-ences in glycemic control in adults with type 2 diabetes 显示文摘 | Harris MI Eastman RC Cowie CC | 1999 | Dlabetes Care1999，，22： | 1 |
| 14 | Targeting lactate- fueled respiration selectively kills hypoxic tumor cells in mice 显示文摘 | Koukourakis MI Giatromanolaki Aj Harris AL | 2008 | J Clin Invest2008，118，12： | 1 |
| 15 | Gestationaldiabetes may repreasent discovery of preexisting glucose intolerance显示文摘 | Harris MI | 1998 | Diabetes Care1998，11，： | 1 |
| 16 | Direct evidence of oxida live injury produced by the Alzheimer's beta amyloid peptide 显示文摘 | Harris ME Hofer MI | 1995 | Exp Neurol1995，131，2： | 1 |
| 17 | Effect of lead acetate on nitrite production by murine brain endothelial cell cultures显示文摘 | Harry GJ luster MI | 1994 | Toxicol Appl Pharmacol1994，126，： | 1 |
| 18 | Racial and ethnic differences in glycemic control in adults with type 2 diabetes显示文摘 | Harris MI Eastman RC Cowie CC | 1999 | Diabetes Care1999，22，： | 1 |
| 19 | ttealth care and health status and outtcomes for pa- tients with type 2 diabetes显示文摘 | Harris MI | 2000 | Diabetes Care2000，23，： | 1 |
| 20 | American Diabetes Association diabetes diagnostic criteria,advancing age,and cardiovascular disease risk profiles:resuIts from the Third National Health and N utrition Examination Survey显示文摘 | Resnick HE Harris MI Brock DB et a1 | 2000 | Diabetes Care2000，23，： | 1 |
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