|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy显示文摘Conventional immunohistochemistry(IHC)is a widely used diagnostic technique in tissue pathology.However,this technique is associated with a number of limitations,including high inter-observer variability and the capacity to label only one marker per tissue section.This review details various highly multiplexed techniques that have emerged to circumvent these constraints,allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition,cellular functional and cell-cell interactions.Among these techniques,multiplex Immunohistochemistry/Immunofluorescence(mIHC/IF)has emerged to be particularly promising.mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible,efficient and cost-effective tissue studies.This technique has immediate potential for translational research and clinical practice,particularly in the era of cancer immunotherapy. | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2020 | Cancer Communications2020，40，4： | 11 |
| 2 | Nature:研究鉴定出蛋白Nlrp9b抵抗轮状病毒感染机制显示文摘轮状病毒(rotavirus)是婴儿和儿童罹患肠胃炎和腹泻的一种最为常见的原因,每年导致全世界大约21.5万人死亡。它是高度传染性的和潜在致命性的。这种病毒特异性地感染宿主小肠中的肠上皮细胞,并且已进化出抵抗干扰素和NF-KB信号的策略来躲避免疫系统,这就提出一个问题:其他的宿主因子是否参与肠道粘膜中的抗病毒反应。不过,针对体内(特别是通过NLR炎性体)检测和限制肠道病毒的机制,人们知之甚少。 | Shu Zhu, Zheng Wei, Noah W. Palm, Yi Yang, Hua Yu, Hua-Bing Li, Geng Wang, Xuqiu Lei, Marcel R. de Zoete, Jun Zhao, Yunjiang Zheng, Haiwei Chen, Liang Shan, Richard A. Flavell Siyuan Ding, Ningguo Feng, Harry B. Greenberg Siyuan Ding, Harry B. Greenberg Penghua Wang, Yujiao Zhao Wen Pan, Jun Lu Jun Zhao, Yuval Kluger Kellie A. Jurado, Clara Abraham, Erol Fikrig Erol Fikrig, Richard A. Flavell | 2017 | 现代生物医学进展2017，17，27： | 10 |
| 3 | Washed microbiota transplantation reduces proton pump inhibitor dependency in nonerosive reflux disease显示文摘BACKGROUND The pathogenesis of gastroesophageal reflux disease(GERD)is closely associated with the intestinal bacteria composition and their metabolites.AIM To investigate whether washed microbiota transplantation(WMT)improves symptoms of nonerosive reflux disease(NERD)with proton pump inhibitor(PPI)dependency.METHODS Patients with recurrent NERD and PPI dependency at the First Affiliated Hospital of Guangdong Pharmaceutical University from 2017 to 2018 were included and divided into a WMT or PPI group treated with PPI with/without WMT.The endpoint was NERD symptom frequency evaluated 1 mo after WMT using reflux disease questionnaire(RDQ)and GERD questionnaire(GERDQ)scores,remission time,PPI dose,and the examination of intestinal mucosal barrier function.RESULTS In the WMT(n=15)and PPI(n=12)groups,the total remission rate at 1 mo after treatment was 93.3%vs 41.7%.Compared with the PPI group,the WMT group showed better results in GERDQ(P=0.004)and RDQ(P=0.003)and in remission months(8 vs 2,P=0.002).The PPI dose was reduced to some extent for 80%of patients in the WMT group and 33.3%in the PPI group.In 24 patients,intestinal mucosal barrier function was examined before treatment,and changes in the degree of damage were observed in 13 of these patients after treatment.Only one of the 15 patients had minor side effects,including a mushy stool two or three times a day,which resolved on their own after 1 wk.CONCLUSION This study is the first to demonstrate that WMT may be safe and effective for relieving NERD symptoms and reducing PPI dependency and recurrence. | Ya-Mei Zheng Xian-Yun Chen Jie-Yi Cai Yu Yuan Wen-Rui Xie Jia-Ting Xu Harry Hua-Xiang Xia Min Zhang Xing-Xiang He Li-Hao Wu | 2021 | World Journal of Gastroenterology2021，27，6： | 7 |
| 4 | 幽门螺杆菌慢性感染诱导胃上皮细胞产生凋亡耐受显示文摘目的 建立幽门螺杆菌 (Hp)慢性感染胃上皮细胞模型 ,并分析Hp慢性感染与胃上皮细胞凋亡的关系。方法 用HpSS1与人非肿瘤性胃上皮细胞株GES 1共培养 16周 ,建立GES 1模型细胞 ,然后采用流式细胞术分析这种模型细胞对Hp和其它肠道细菌以及抗肿瘤药物凋亡诱导剂的应答。结果 成功获得了Hp慢性感染胃上皮细胞模型 (GES 1模型细胞 )。这种模型细胞不仅对Hp诱导的凋亡耐受 ,而且对其他肠道细菌和一些凋亡诱导剂也是耐受的。结论 幽门螺杆菌慢性感染诱导胃上皮细胞产生凋亡耐受 ,由此可能增加了胃上皮细胞癌变的危险性。 | 何兴祥 胡品津 Benjamin Chun Yu Wong Shiu Kum Lam Harry Hua Xiang Xia | 2003 | 中华医学杂志2003，83，1： | 6 |
| 5 | A novel discrete model for granular material incorporating rolling resistance显示文摘 | M.J. Jiang H.-S. Yu D. Harris | 2005 | Computers and Geotechnics2005，，5： | 3 |
| 6 | 肿瘤免疫治疗时代的多重免疫组织化学/免疫荧光技术显示文摘常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单张切片上同时检测多个标志物,可对细胞组成、细胞功能和细胞间相互作用进行整合研究。在这些技术中,多重免疫组织化学/免疫荧光(multiplex immunohistochemistry/immunofluorescence,mIHC/IF)已成为最具应用前景的技术。mIHC/IF为开展高重复性、高效、高性价比的组织研究提供了高通量多重染色技术和标准化的定量分析。该技术在转化研究和临床实践中,尤其是肿瘤免疫治疗中极具应用价值。 | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2021 | 癌症2021，40，9： | 2 |
| 7 | Variance decompositions of nonlinear dynamic stochastic systems 显示文摘 | Harris T J Yu W | 2010 | Journal of Process Control2010，20，2： | 1 |
| 8 | Controller assessment for a class of nonlinear systems 显示文摘 | Harris T J Yu W | 2007 | Journal of Process Control2007，17，7： | 1 |
| 9 | High-sensitivity C-reactive protein and at hersclerosis; from theory to the reply 显示文摘 | Harry Yu Nader Rifai | 2000 | Clinical Bio-chemist ry2000，33，8： | 1 |
| 10 | Saturation and compar ative mapping of genornics region harboring hessian fly resist ance gene H26 in wheat显示文摘 | Yu G T Cao X W Harris MO etal | 2009 | Theoretical and Applied Genetics2009，118，： | 1 |
| 11 | Comparative epidemiology of cancers of the colon,rectum,prostate and breast in Shanghai, China versus the United States 显示文摘 | Yu H Harris RE Gao YT | 1991 | Int J Epidemiol1991，20，： | 1 |
| 12 | High-sensitivity C-reactive protein and atherosclerosis:From theory to therapy显示文摘 | Harry Yu Nader Rifai | 2000 | Clinical Biochemistry2000，33，8： | 1 |
| 13 | Ch OpenCV for interactive open architecture computer vision显示文摘 | Qingcang Yu Harry H Cheng Wayne W Cheng | 2004 | Advances in Engineering Software2004，35，9： | 1 |
| 14 | Bond rolling resistance and its effect on yielding of bonded granulates by DEM analyses显示文摘 | JIANG M J YU H S HARRIS D | 2006 | International Journal for Numerical and Analytical Methods in Geomechanics2006，30，7： | 1 |
| 15 | High-sensitivity C-reactive protein and athersclerosis:from theory to therapy显示文摘 | Nader Rifai | 2000 | Clinical Biochemistty2000，33，8： | 1 |
| 16 | Control performance assessment for Hammerstein-Wiener models显示文摘 | Yu W Wilson D I Harris T J | 2010 | Dynamics and Control of Process Sys- tems2010，11，1： | 1 |
| 17 | Characteristics of Pseudoexfoliation in the Thessaloniki Eye Study显示文摘 | Eleftherios Anastasopoulos Fotis Topouzis M. Roy Wilson Alon Harris Theofanis Pappas Fei Yu Archimidis Koskosas Panayiota Founti Anne L. Coleman | 2011 | Journal of Glaucoma2011，，3： | 1 |
| 18 | Discrete element modelling of deep penetration in granular soils显示文摘 | Jiang M J Yu H S Harris D | 2006 | International Journal for Numerical and Analytical Methods in Geomechanics2006，30，2： | 1 |
| 19 | A novel discrete model granular material incorporating rolling resistance显示文摘 | JIANG M J YU H S HARRIS D | 2005 | Computers and Geotechnics2005，32，： | 1 |
| 20 | Web-Based Control System Design and Analysis显示文摘 | Qingcang Yu Bo Chen Harry H Cheng | 2004 | IEEE Control Systems Magazine2004，24，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员