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| 1 | Regulatory effect and mechanism of gastrin and its antagonists on colorectal carcinoma显示文摘AIM To explore the effect and mechanism ofgastrin and its antagonists proglumide and somatostatin on coIorectal carcinoma and their clinical significance.METHODS A model of transplanted human colonic carcinoma was established from SW480cell line in gymnomouse body. The volume andweight of transplanted carcinoma was observedunder the effect of pentagatrin (PG), proglumide (PGL) and octapeptide somotostatin (SMS201-995, SMS). The cAMP content ot carcinoma cell was determined by redioimmunoassay and the DNA, protein content and cell cycle were determined by flow-cytometry. The amount of viabIe cells was determined by MTT colorimetric analysis, lP3 content was determined by radioimmunoassay, Ca2+ concentration in cell by fluorometry and PKC activity by isotopic enzymolysis. The expression of gastrin, c-myc,C-fos and rasP21 in 48 cases of colorectal carcinoma tissue was detected by the immunocytochemistry SP method. Argyrophilianucleolar organizer regions was determined withargyrophilia stain.RESULTS The volume, weight, cAMP, DNAand protein content in carcinoma cell, cellamount and proliferation index of S and G2Mphase in PG group were all significantly higher than those of control group. When PG was at theconcentration of 25 mg/ L, the amount of viablecells, lP3 content and Ca2+ concentration in celland membrane PKC activity in PG group weresigniticantly higher than those in control group;when PGL was at a concentration ot 32 mg/L,they dropped to the lowest level in PG (25 mg/L)+ PGL group, but without significant differencefrom the control group. The positive expression rate of gastrin, c-myc, c-fos and rasp21 in carcinoma tissue was 39 .6%, 54 .2%, 47. 9% and54 .2% resPectively and significantly higher than that in mucosa 3 cm and 6 cm adjacent tocarcinoma tissue and normal colorectal mucosa.The positive expression rate of gastrin of highlydifferentiated adenocarcinoma group was significantly higher than that of poorly differentiated and mucinous adenocarcinoma groups. The AgNORs count of carcinoma tissue was significantly higher than that in mucosa 3 cm and 6 cm adjacent to carcinoma tissue and normal colorectal mucosa; and the positive expression of c-myc and c-fos and the AgNORs count in gastrin-positive group was significantly higher than those in gastrin-negative group.CONCLUSION Pentagastrin has a promoting effect on the growth of transplanted human colonic carcinoma from SW480 cell line. PGL hasno obvious effect on the growth of human colonic carcinoma SW480 cell line, but couldinhibit the growth-promoting effect of PG on transplanted carcinoma. Somatostatin can not only inhibit the growth of transplanted human colonic carcinoma from SW480 cell line directlybut also depress the growth-promoting effect ofgastrin on the transplanted carcinoma. Somecolorectal carcinoma cells can produce and secrete gastrin through autocrine, highly-differentiated adenocarcinoma express the highest level gastrin. Endogenous gastrin can stimulate the cell division and proliferation of carcinoma cell and promote the growth of colorectal carcinoma regulating the expression of oncogene omyc, c-fos. Our study has provided experimental basis for the adjuvant treatment using gastrin antagonist such as PGL,somatostatin of patients with colorectalcarcinoma. | He SW Shen KQ He YJ Xie B Zhao YM | 1999 | World Journal of Gastroenterology1999，5，5： | 20 |
| 2 | NGF promotes collateral sprouting of cholinergic fibers in the septo-hippocampal cholinegic system of aged rats with fimbria transection显示文摘 | He YS Yao ZB Gu YM | 1992 | Brain Res1992，586，： | 2 |
| 3 | NGF promotes collateral prouting of cholinergic fibers in the septohippocampal cholinergic system of aged rats with fimbria transection 显示文摘 | HE YS Yao ZB Gu YM | 1992 | Brain Res1992，586，： | 1 |
| 4 | Prospective feasibility study on the use of multiband mucosectomy for endoscopicresection of early squamous neoplasia in the esophagus显示文摘 | Zhang YM Boerwinkel DF He S | 2013 | Endoscopy2013，45，： | 1 |
| 5 | Freestanding three-dimensional graphene/Mn O2composite networks as ultralight and flexible supercapacitor electrodes显示文摘 | He YM Chen WJ Li XD | 2013 | ACS Nano2013，7，： | 1 |
| 6 | Recombinant adenovirus- mediated hu- man telomerase reverse transcriptase gene can stimulate cell proliferation and maintain primitive characteristics in bovine manmaary gland epithelial cells 显示文摘 | HE XY ZHENG YM LAN J | 2011 | Development Growth & Differentiation2011，53，3： | 1 |
| 7 | Antioxidant activity of microwave assisted extract of Buddleia officinalis and its major active component显示文摘 | Pan YM He CH Wang HS | 2010 | Food Chem2010，121，： | 1 |
| 8 | Taspine isolated from Radix et Rhizoma Leonticis inhibits growth of human umbilical vein endothelial cell by inducing its apoptosis 显示文摘 | Zhang YM He LC Zhou YL | 2008 | Phyto- med/cine2008，15，12： | 1 |
| 9 | Treatment of idiopathic membranous nephropathy with combination of low-dose ta- crolimus and corticosteroids显示文摘 | He LY Peng YM Liu H | 2013 | J Nephrol2013，26，3： | 1 |
| 10 | Neural respiratory drive and ventilation in patients with chronic obstructive pulmonary disease during sleep显示文摘 | Luo YM He BT Wu YX | 2014 | Am J Respir Crit Care Med2014，190，2： | 1 |
| 11 | Nerve growth factor promotes collateral sprouting of cholinergic filbers in the septohippocampal cholinergic system of aged rats with fimbria transaction 显示文摘 | He YS Yao ZB Gu YM | 1992 | Brain Res1992，586，1： | 1 |
| 12 | Involvement of Escherichia coli K1 ibeT in bacterial adhesion that is associated with the entry into human brain microvascular endothelial cells显示文摘 | Zou YM He LN Chi F | 2008 | Medical Microbiology and Immunology2008，197，4： | 1 |
| 13 | Synthesis of g-C3NffSmVO4 Composite Photocatalyst with Improved Visible Light Photocatalytic Activities in RhB Degradation显示文摘 | Li TT Zhao LH He YM | 2013 | Applied Catalysis B: Environmental2013，129，： | 1 |
| 14 | Shared genetic determinant of axial length, anterior chamber depth, and angle opening distance: The Guangzhou Twin Eye Study显示文摘 | He M Hur YM Zhang J | 2008 | Invest Ophthalmol Vis Sci2008，49，11： | 1 |
| 15 | Toxicity ofprofenofos to the springtail,Folsomia candida,and ammonia-oxidizers in two agricultural soils显示文摘 | Liu YR Zheng YM He J Z | 2012 | Ecotoxicology2012，21，4： | 1 |
| 16 | Effects of SMYD3 over - ex-pression on cell cycle acceleration and cell proliferation in MDA -MB -231 human breast cancer cells 显示文摘 | Ren TN Wang JS He YM el al | 2011 | Med Oncol2011，28，1： | 1 |
| 17 | Molecular detection of three gastroenterifis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria 显示文摘 | HE XQ WEI YM CHENG L | 2012 | Environ Monit Assess2012，184，9： | 1 |
| 18 | Strain Specificity of Clinical isolates of herpes simplex virus Arch显示文摘 | Wander AH Centifanto YM Kaufman HE | 1980 | Opthalmol1980，98，： | 1 |
| 19 | NGF promotes collateral sproutingof cholinergic fibers in the septo-hippocampal cholinegic systemof aged rats with fimbria transection 显示文摘 | He YS Yao ZB Gu YM | 1992 | Brain Res1992，586，： | 1 |
| 20 | Distribution of diego blood group alleles and identification of four novel mutations on exon 19 of SLC4A1 gene in the Chinese Han population by polymerase chain reaction sequence-based typing显示文摘 | Xu XG He J He YM | 2011 | Vox Sang2011，100，3： | 1 |
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