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1Population-based study of DNA image cytometry as screening method for esophageal cancer显示文摘AIM:To explore the DNA image cytometry (DNA-ICM) technique as a primary screening method for esopha-geal squamous precancerous lesions.METHODS:This study was designed as a population-based screening study.A total of 582 local residents aged 40 years-69 years were recruited from Linzhou in Henan and Feicheng in Shandong.However,only 452 subjects had results of liquid-based cytology,DNA-ICM and pathology.The sensitivity and specificity of DNA-ICM were calculated and compared with liquid-based cytology in moderate dysplasia or worse.RESULTS:Sensitivities of DNA-ICM ranging from at least 1 to 4 aneuploid cells were 90.91%,86.36%,79.55% and 77.27%,respectively,which were better than that of liquid-based cytology (75%).Specifici-ties of DNA-ICM were 70.83%,84.07%,92.65% and 96.81%,but the specificity of liquid-based cytology was 91.91%.The sensitivity and specificity of a combination of liquid-based cytology and DNA-ICM were 84.09% and 85.78%,respectively.CONCLUSION:It is possible to use DNA-ICM tech-nique as a primary screening method for esophageal squamous precancerous lesions.Lin Zhao,Wen-Qiang Wei,Xin-Qing Li,Guo-Qing Wang,Qi Shang,You-Lin Qiao,Department of Cancer Epidemiology,Cancer Institute/Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China De-Li Zhao,Department of Epidemiology,Feicheng People’s Hospital,Feicheng 271600,Shandong Province,China Chang-Qing Hao,Department of Endoscopy,Linzhou Cancer Hospital,Linzhou 456500,Henan Province,China Dong-Mei Lin,Qin-Jing Pan,Department of Pathology,Cancer Institute/Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China Fu-Hua Lei,Department of Pathology,Feicheng People’s Hospi-tal,Feicheng 271600,Shandong Province,China Jin-Wu Wang,Department of Pathology,Linzhou Cancer Hospi-tal,Linzhou 456500,Henan Province,China 2012World Journal of Gastroenterology2012,18,4:15
2Roux-en-Y gastric bypass promotes expression of PDX-1 and regeneration of β-cells in Goto-Kakizaki rats显示文摘AIM:To study the effects of Roux-en-Y gastric bypass(RYGB) on the expression of pancreatic duodenal homeobox-1(PDX-1) and pancreatic β-cell regeneration/neogenesis,and their possible mechanisms in diabetics.METHODS:Three groups of randomly selected nonobese diabetic Goto-Kakizaki(GK) rats were subjected to RYGB,sham-RYGB and sham-operation(sham-op) surgery,respectively.The rats were euthanized at postoperative 1,2,4 and 12 wk.Their pancreases were resected and analyzed using reverse transcription polymerase chain reaction to detect the mRNA of PDX-1.Anti-PDX-1 immunohistochemical(IHC) staining and Western blotting were used to detect the protein of PDX-1.Double IHC staining of anti-Brdu and-insulin was performed to detect regenerated β-cells.The index of double Brdu and insulin positive cells was calculated.RESULTS:In comparison with sham-RYGB and sham-op groups,a significant increase in the expressions of PDX-1 mRNA in RYGB group was observed at all experimental time points(1 wk:0.378 ± 0.013 vs 0.120 ± 0.010,0.100 ± 0.010,F = 727.717,P < 0.001;2 wk:0.318 ± 0.013 vs 0.110 ± 0.010,0.143 ± 0.015,F = 301.509,P < 0.001;4 wk:0.172 ± 0.011 vs 0.107 ± 0.012,0.090 ± 0.010,F = 64.297,P < 0.001;12 wk:0.140 ± 0.007 vs 0.120 ± 0.010,0.097 ± 0.015,F = 16.392,P < 0.001);PDX-1 protein in RYGB group was also increased significantly(1 wk:0.61 ± 0.01 vs 0.21 ± 0.01,0.15 ± 0.01,F = 3031.127,P < 0.001;2 wk:0.55 ± 0.00 vs 0.15 ± 0.01,0.17 ± 0.01,F = 3426.455,P < 0.001;4 wk:0.39 ± 0.01 vs 0.18 ± 0.01,0.22 ± 0.01,F = 882.909,P < 0.001;12 wk:0.41 ± 0.01 vs 0.20 ± 0.01,0.18 ± 0.01,F = 515.833,P < 0.001).PDX-1 mRNA and PDX-1 protein production showed no statistical significance between the two sham groups.Many PDX-1 positive cells could be found in the pancreatic islets of the rats in RYGB group at all time points.In addition,the percentage of Brdu-insulin double staining positive cells was higher in RYGB group than in the other two groups(1 wk:0.22 ± 0.13 vs 0.03 ± 0.06,0.03 ± 0.06,P < 0.05;2 wk:0.28 ± 0.08 vs 0.00 ± 0.00,0.03 ± 0.06,P < 0.05;4 wk:0.24 ± 0.11 vs 0.07 ± 0.06,0.00 ± 0.00,P < 0.001;12 wk:0.20 ± 0.07 vs 0.03 ± 0.06,0.00 ± 0.00,P < 0.05).CONCLUSION:RYGB can increase the expression of pancreatic PDX-1 and induce the regeneration of β-cells in GK rats.The associated regeneration of islet cells may be a possible mechanism that how RYGB could improve type 2 diabetes mellitus.Zhen Li,Dong-Fei Li,Jing-Xing Dai,Yu Bai,Lin Yuan,Southern Medical University,Institute of Basic Medical Anatomy National Key Disciplines,Guangzhou 510515,Guangdong Province,China Hong-Ya Zhang,Lu-Xian Lv,Wen-Qiang Li,Second Affiliated Hospital,Xinxiang Medical College,Henan Province Key Laboratory of Biological Psychiatry,Xinxiang 453002,Henan Province,China Ou Sha,Department of Anatomy,Faculty of Medicine,Shenzhen University,Shenzhen 518060,Guangdong Province,China 2010World Journal of Gastroenterology2010,16,18:14
3Quick image-processing method of UAV without control points data in earthquake disaster area显示文摘The methods of unmanned aerial vehicles images splicing rapidly and rectifying without control points data, only using auxiliary data recorded by unmanned aerial vehicle (UAV) system, were researched. Two algorithms were proposed, including UAV images auto- splicing rapidly after image blocking and UAV images rectifying based on pseudo center points of auxiliary data. When SIFT algorithm for UAV images splicing is applied, the accuracy is very high, but the efficiency is very low. The splicing efficiency is improved greatly based on SIFT algorithm after image blocking. The results show that rectifying accuracy is very high and has no visual dislocation when pseudo center points of auxiliary data is applied.LI Chang-chun1, ZHANG Guang-sheng1, LEI Tian-jie2, 3, GONG A-du2, 3 1. School of Surveying & Land Information Engineering, Henan Polytechnic University, Jiaozuo 454000, China 2. Key Laboratory of Environmental Change and Natural Disaster, Ministry of Education, Beijing Normal University, Beijing 100875, China 3. Ministry of Civil Affairs/Ministry of Education of China Academy of Disaster Reduction and Emergency Management, Beijing Normal University, Beijing 100875, China 2011中国有色金属学会会刊:英文版2011,21,S3:13
4Relationships between bacterial-algal proliferating and mass extinction in the Late Devonian Frasnian-Famennian transition:Enlightening from carbon isotopes and molecular fossils显示文摘Studies show positive shifts of inorganic and organic carbon isotope values (δ 13Ccarb and δ 13Ckerogen) from +0.43 (‰ V-PDB) to +3.54 (‰ V-PDB) and from ?29.38 (‰ V-PDB) to ?24.14 (‰ V-PDB), respectively, B* (Ba* = Ba/ (Al2O3 X 15%)) values from 0.015 to 0.144, TOC values from 0.02% to 0.21%, V/Cr values from 0.3 to 2.0, Sr/Ba values from 3.20 to 49.50 in the Late Devonian Frasnian Upper rhenana zone to the top linguiformis zone of the Yangdi sec-tion deposited in carbonate slope facies of Guilin, Guangxi, South China, which indicates that biomass, productivity, organic carbon burial and salinity increase and that oxygenation near the boundary between sediments and waters decreases from the Late Devonian Frasnian Upper rhenana zone to the top linguiformis zone. Abundance of molecular fossils increases and normal alkanes, isoprenoid hydrocarbon, terpanes and steranes are dominated from the Late Devonian Frasnian to the bottom of Famennian, which shows that the predecessors of molecular fossils of the Frasnian-Famennian (F-F) transition are dominated by marine phytoplankton, zooplankton and benthic bacteria with no photosynthesis. Therefore, it is considered that the F-F transitional mass extinction with a multistage, selection and global synchronizing was caused by bacte-rial-algal proliferating, continuing deterioration of the shallow marine ecoenvironment of the mid-dle-lower latitudes. A simple cause and effect chain can be expressed as: appearance of seed plants and multi-storied forests → enhanced chemical and biochemical weathering and pe-dogenesis → wide development of soils → increasing riverine nutrient fluxes in epicontinental sea → from superoligotrophic to eutrophic in epicontinental sea → proliferating of marine phyto-plankton and zooplankton → frequent red tide and anoxia → mass extinction of shallow marine organisms in the middle-lower latitudes. It is worth notice that the factor drawdown of atmos-pheric Pco2, climatic cooling and sea level falling caused by eutrophication, anoxia and organic carbon burial increasing may be important for the mass extinction.GONG Yiming1,2, XU Ran1, TANG Zhongdao1, SI Yuanlan3 & LI Baohua4 1. Faculty of Earth Science and State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Wuhan 430074, China 2. Institute of Resources & Environments, Henan Polytechnic University, Jiaozuo 454003, China 3. Department of Computer Science, South-Central University for Nationalities, Wuhan 430074, China 4. Laboratory of Marine Geology, Tongji University, Shanghai 200092, China 2005Science China Earth Sciences2005,48,10:13
5Semigroup Method for a Mathematical Model in Reliability Analysis显示文摘The system which consists of a reliable machine, an unreliable machine and a storage buffer with infinite many workpieces has been studied. The existence of a unique positive time-dependent solution of the model corresponding to the system has been obtained by using C 0-semigroup theory of linear operators in functional analysis.Geni Gupur 1,3 , LI Xue-zhi 2,3 1.Department of Mathematics, Xinjiang University, Urumqi 830046,China2. Department of Mathematics, Xinyang Teachers College, Henan 464000, China3. Academy of Mathematics and System Sciences, Chinese Academy of Sci 2001Journal of Systems Science and Systems Engineering2001,13,2:12
6Human papillomavirus DNA and P16~(INK4A) expression in concurrent esophageal and gastric cardia cancers显示文摘AIM:To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16 INK4A protein expression.METHODS:Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16).The expression of P16 INK4A protein was detected using immunohistochemistry.RESULTS:Among the CC specimens,HPV16-DNA was found in eight cases of esophageal squamous cell carcinoma (ESCC) and five cases of gastric cardia adenocarcinoma (GCA),respectively (47% vs 29%),and two of both ESCC and GCA.P16 INK4A was highly expressed in both ESCC and GCA.In the HPV-associated positive CC,higher P16 INK4A expression was observed in the GCA than in the ESCC (75% vs 25%,P < 0.05).CONCLUSION:HPV16 as a correlated risk factor may play an important role in the development of ESCC and GCA.P16 INK4A may be a screening index in the HPVassociated carcinoma of gastric cardia.Guang-Cheng Ding,Tao Guo,Department of Gastroenterology,The First Affiliated Hospital and The Fifth Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,Henan Province,China Jing-Li Ren,Xin Song,Sheng-Li Zhou,Zong-Min Fan,LiDong Wang,Henan Key Laboratory for Esophageal Cancer Research,Department of Gastroenterology,The First Affiliated Hospital,College of Basic Medicine,Zhengzhou University,Zhengzhou 450052,Henan Province,China Fu-Bao Chang,Department of Thoracic Surgery,Linzhou Center Hospital,Linzhou 456500,Henan Province,China Ji-Lin Li,Department of Pathology,Yaocun Esophageal Cancer Hospital,Linzhou 456500,Henan Province,China Ling Yuan,Department of Oncoradiotherapy,Henan Province Tumor Hospital,Zhengzhou 450003,Henan Province,China Yi Zeng,Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China 2010World Journal of Gastroenterology2010,16,46:11
7Effect of rare-earth element Sm on the corrosion behavior of Mg-6Al-1.2Y-0.9Nd alloy显示文摘The effect of Sm(0 wt.%-2 wt.%) addition on the corrosion behavior of Mg-6Al-1.2Y-0.9Nd alloy in 3.5 wt.% NaCl solution has been studied by static corrosion tests,corrosion morphology observation,corrosion scale and microstructure analysis.The results show that,with the increasing of Sm content,the corrosion rate of the alloy decreases at first,then increases and reaches the valley at 0.5 wt.% Sm.The reason is that the proper content of Sm addition can refine the precipitates and make the component and microstructure uniform,and therefore,it remarkably improves the corrosion resistance of the alloy.LI Quan’an,LI Xiaofeng,ZHANG Qing,and CHEN Jun School of Materials Science and Engineering,Henan University of Science and Technology,Luoyang 471003,China 2010Rare Metals2010,29,6:11
8Heat shock induction of a 65 kDa ATP-binding proteinase in rat C6 glioma cells显示文摘The 45, 55, 65 and 100 kDa ATP-binding proteinases (ATP-BPases) of the heat-shocked (44 ℃ for 30 min, recovery for 12h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography. Their molecular masses, isoelectric points (pI), pH-optima and other properties were analyzed by native proteinase gels.It was shown that the 65 kDa ATP-BPase is specifically induced by heat shock and not detectable in control cells.Its N-terminal 1-9 amino acid sequence was determined by Edman degradation, but no homologies to other proteins in the protein data bases were found. 30 and 31 kDa proteinases can be cleaved from the 45, 55 and 65 kDa proteinases to which they are linked. A possible relationship of the heat-induced 65 kDa ATP-BPase with the ATP-dependent proteinases (ATP-DPases) in prokaryotes and eukaryotes is discussed.XU CUN SHUAN WEI MING ZHANG DIETER TECHEL MARCO MEYER YAN ZHANG LI LUDGER RENSING (Department of Biology, Henan Normal University,Xinxiang 453002)(Institute of Cell Biology, Bremen University, D-28359 Bremen, Germany) 1999Cell Research1999,9,2:8
9An Efficient Method for the Synthesis of Acyl-diazenes Using NaNO_2/NaHSO_4·H_2O显示文摘Jian Ping LI*, Ping LIU, Yu Lu WANG College of Chemistry & Environmental Science, Henan Normal University, Xinxiang 453002 Key Laboratory of Environmental Science and Technology of High Education of Henan Province, Xinxiang 453002 2003Chinese Chemical Letters2003,14,7:7
10TEM Study on the Formation Process of TiO_2 Nanotubes显示文摘The process, that the polycrystalline TiO2 powders were converted into TiO2 nanotubes, was observed with transmission electron microscope. The results obtained indicated that in concentrated NaOH aqueous solution, anisotropic swelling appears on the polycrystalline TiO2 granula at first, and then the nanotubes are formed.Jing Wei ZHANG, Xin Yong GUO, Zhen Sheng JIN*, Shun Li ZHANG, Jing Fang ZHOU, Zhi Jun ZHANG* Lab of Special Functional Materials, Henan University, Kaifeng 475001 2003Chinese Chemical Letters2003,14,4:7
11Temperature dependence of the point defect properties of GaN thin films studied by terahertz time-domain spectroscopy显示文摘The dielectric functions of GaN for the temperature and frequency ranges of 10–300 K and 0.3–1 THz are obtained using terahertz time-domain spectroscopy.It is found that there are oscillations of the dielectric functions at various temperatures.Physically,the oscillation behavior is attributed to the resonance states of the point defects in the material.Furthermore,the dielectric functions are well fitted by the combination of the simple Drude model together with the classical damped oscillator model.According to the values of the fitting parameters,the concentration and electron lifetime of the point defects for various temperatures are determined,and the temperature dependences of them are in accordance with the previously reported result.Therefore,terahertz time-domain spectroscopy can be considered as a promising technique for investigating the relevant characteristics of the point defects in semiconductor materials.FANG HeNan ZHANG Rong LIU Bin LI YeCao FU DeYi LI Yi XIE ZiLi ZHUANG Zhe ZHENG YouDou WU JingBo JIN BiaoBing CHEN Jian WU PeiHeng 2013Science China(Physics,Mechanics & Astronomy)2013,56,11:6
12The New Synthesis of Azo Urea from Substituted Semicarbazide显示文摘Using N-bromosuccinimide and pridine as the 0xidation s}-stem to dehydrogenale anly substituted semicarbazide to form azo compounds is described for the firs1 time in this paper Nine azo conlpounds have been prepared in excellent lield under mild condition This method onle needs simple instrument and short reactionXiao Yang WANG Yu Lu WANG Jian Ping LI Cai ’Lan W’ANG Hong WANG (Department of Chemistry ,Henan Normal University Xinxiang 453002 ) 1997Chinese Chemical Letters1997,8,6:5
13Synthesis,characterization,properties and crystal structure of heterometallic 1D coordination polymers {[CuLZn·CuLZn(H_2O)]·H_2O}_n显示文摘Copper-zinc heterometallic 1D chain coordination polymer has been synthesized and characterized by elemental analysis, and IR spectra etc. The crystal structure was determined by single-crystal X-ray diffraction analyses. The title complex is 1D chain coordination polymer with the chemical formula {[CuLZn·CuLZn(H2O)]·H2O}n, where H4L=N-(2-hydroxybenzamido)-N’-(3-carboxy- lsalicylidene)ethylenediamine. Its structural unit is comprosed of two tetranuclear cycles formed by two dissymmetrical tetranuclear units. These units polymerized each other to form 1D chain coordi- nation polymer.TAO Ruojie1, LI Fu’an1, ZANG Shuangquan1, CHENG Yanxiang2 & NIU Jingyang1 1. Chemistry and Chemical Engineering College of Henan University, Kaifeng 475001, China 2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China 2006Science China Chemistry2006,49,4:5
14Testing of tuberculosis infection among Chinese adolescents born after terminating the Bacillus Calmette-Guerin booster vaccination: subgroup analysis of a population-based cross-sectional study显示文摘Hengjing Li Henan Xin Shukun Qian Xiangwei Li Haoran Zhang Mufei Li Boxuan Feng Qi Jin Lei Gao 2017Frontiers of Medicine2017,11,4:5
15Countermeasures and Investigation of Land Circulation in Less Developed Agricultural Area of Henan Province,China显示文摘Some places in Henan Province are investigated by using sample surveys and interviews. Result shows that land circulation in less developed agricultural area of Henan Province has the characteristics of small scale,single form,short period,and circulation land in economic core region. Problems in land circulation are as follows:slow transfer of rural surplus labor force,hard implementation of fundamental land circulation,complicated mentality towards rural land circulation,many misgivings about land circulation,the unformed social service system of land circulation,and imperfect agricultural risk prevention mechanism for circulated land use. Based on the characteristics of land circulation,suggestions for promoting the land circulation of less developed agricultural areas are put forward in order to solve the problems in land circulation and to lay the foundation for effective implementation of land circulation,such as speeding up the development of urbanization,developing the circulation of collective land,effectively implementing the laws and regulations of rural collective land circulation,transferring land according to law,establishing market mechanism of agricultural land circulation,promoting the agricultural industrialization,establishing market mechanism of agricultural land circulation,promoting the agricultural industrialization,energetically readjusting rural industrial structure,and developing economy with local characteristics.LI Cong-guo College of Environment and Planning,Henan University,Kaifeng 475004,China 2010Asian Agricultural Research2010,2,4:5
16Use-wear analysis confirms the use of Palaeolithic bone tools by the Lingjing Xuchang early human显示文摘During 2007-2008 excavations at the Lingjing site near Xuchang,Henan Province,dated back to around 100-80 ka ago,a large quantity of mammalian fossil remains were recovered along with a remarkable cluster of Early Modern Human(EMH) skull fragments in situ.Observably some of those animal bones were probably modified into tools.A use-wear analysis was carried out to examine the functions of modified bone tools.The results suggest that Lingjing bone tools were used for drilling,penetrating,and scraping animal substances,and that some might have been hafted during the use.This study confirms that early existence ofintentionally-modified bone tools at human occupations of the early Late Pleistocene in northern China.This discovery suggests making and use of bone tools were inevitably a part of early human behaviors and cultural development,as such of stone tools.LI ZhanYang 1,4 & SHEN Chen 2,3,4 1 Henan Provincial Institute of Cultural Relics and Archaeology,Zhengzhou 450000,China 2 The Joint Laboratory of Human Evolution and Archaeometry,Chinese Academy of Sciences,Beijing 100044,China 3 The Royal Ontario Museum,Toronto ON M5S2C6,Canada 4 Oriental Archaeology Research Center of Shandong University,Jinan 250100,China 2010Chinese Science Bulletin2010,55,21:4
17Biological and chemical diversity of cytotoxin-producing symbiotic marine fungi in intertidal zone of Dalian显示文摘In this study, the biological and chemical diversity of 8 symbiotic marine fungal strains, with strong cytotoxicity against brine shrimp larvae, were investigated by nucleotide sequencing, morphology and cluster analysis of HPTLC fingerprint. These strains were identified by ITS rDNA sequencing, phylogenic analysis, and morphology to be Hypocrea lixii, Chaetomium globosum, Aspergillus fumigatus, Asp. clavatus and Alternaria sp. Their differences in secondary metabolites were shown by cluster analysis of digitalized colors of HPTLC spots, a newly developed method, which produced a similar dendrogram with that of ITS cluster analysis. Furthermore, this method can fully display intraspecific differences and even the remarkable difference in Aspergillus strains which goes beyond the boundary between genera. Their biological-chemical diversity may be the basis of their potent cytotoxicity and implies their potential in producing diversified antitumor or pesticidal constituents.ZHANG Yi MU Jun FENG Yan LI HeNan DONG XueWei 2013Chinese Science Bulletin2013,58,19:4
18Hollow cobalt oxide nanoparticles embedded in nitrogen-doped carbon nanosheets as an efficient bifunctional catalyst for Zn–air battery显示文摘Rational design of low-cost, highly electrocatalytic activity, and stable bifunctional electrocatalysts for oxygen reduction reaction(ORR) and oxygen evolution reaction(OER) has been a great significant for metal–air batteries. Herein, an efficient bifunctional electrocatalyst based on hollow cobalt oxide nanoparticles embedded in nitrogen-doped carbon nanosheets(Co/N-Pg) is fabricated for Zn–air batteries. A lowcost biomass peach gum, consisting of carbon, oxygen, and hydrogen without other heteroatoms, was used as carbon source to form carbon matrix hosting hollow cobalt oxide nanoparticles. Meanwhile, the melamine was applied as nitrogen source and template precursor, which can convert to carbon-based template graphitic carbon nitride by polycondensation process. Owing to the unique structure and synergistic effect between hollow cobalt oxide nanoparticles and Co-N-C species, the proposal Co/N-Pg catalyst displays not only prominent bifunctional electrocatalytic activities for ORR and OER, but also excellent durability. Remarkably, the assembled Zn–air battery with Co/N-Pg air electrode exhibited a low discharge-charge voltage gap(0.81 V at 50 mA cm^-2) and high peak power density(119 mW cm^-2) with long-term cycling stability. This work presents an effective approach for engineering transition metal oxides and nitrogen modified carbon nanosheets to boost the performance of bifunctional electrocatalysts for Zn–air battery.Yuhui Tian Li Xu Jian Bao Junchao Qian Huaneng Su Huaming Li Haidong Gu Cheng Yan Henan Li 2019Journal of Energy Chemistry2019,28,6:4
19Influence of diet intake on liver function test显示文摘InfluenceofdietintakeonliverfunctiontestCHENGShuQuan,ZHANGJiFang,ZHANGZiFu,QIANMeiYan,GUOXiaoLing,SHANGWenZhangandLIDuo...CHENG Shu Quan, ZHANG Ji Fang, ZHANG Zi Fu, QIAN Mei Yan, GUO Xiao Ling, SHANG Wen Zhang and LI Duo Jing Department of Medical Laboratory, The Central Hospital of Jiaozuo Coal Mine Administration, Jiaozuo 454150, Henan Province, China 1997World Journal of Gastroenterology1997,3,4:3
20Single nucleotide polymorphisms in chicken lmbr1 gene were associated with chicken growth and carcass traits显示文摘Lmbr1 is the key candidate gene controlling vertebrate limb development, but its effects on animal growth and carcass traits have never been reported. In this experiment, lmbr1 was taken as the candi-date gene affecting chicken growth and carcass traits. T/C and G/A mutations located in exon 16 and one A/C mutation located in intron 5 of chicken lmbr1 were detected from Silky, White Plymouth Rock broilers and their F2 crossing chickens by PCR-SSCP and sequencing methods. The analysis of vari-ance (ANOVA) results suggests that T/C polymorphism of exon 16 had significant association with eviscerated yield rate (EYR), gizzard rate (GR), shank and claw rate (SCR) and shank girth (SG); A/C polymorphism of intron 5 was significantly associated with SCR, liver rate and head-neck weight (HNW), while both sites had no significant association with other growth and carcass traits. These results demonstrate that lmbr1 gene could be a genetic locus or linked to a major gene significantly affecting these growth and carcass traits in chicken.HUANG YanQun1,2,3, DU XiaoHui2, DENG XueMei4, QIU XiangPin2, WANG ChunKao4, CHEN Wen3, LI Ning1 & WU ChangXin4 1 State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China 2 College of Animal Science, Sichuan Agricultural University, Ya’an 625014, China 3 College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China 4 College of Animal Science and Technology, China Agricultural University, Beijing 100094, China 2007Science China(Life Sciences)2007,50,1:3
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