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| 1 | Bioengineered miR-27b-3p and miR-328-3p modulate drug metabolism and disposition via the regulation of target ADME gene expression显示文摘Drug-metabolizing enzymes, transporters, and nuclear receptors are essential for the absorption, distribution, metabolism, and excretion(ADME) of drugs and xenobiotics. MicroRNAs participate in the regulation of ADME gene expression via imperfect complementary Watson–Crick base pairings with target transcripts. We have previously reported that Cytochrome P450 3A4(CYP3A4) and ATP-binding cassette sub-family G member 2(ABCG2) are regulated by miR-27b-3p and miR-328-3p,respectively. Here we employed our newly established RNA bioengineering technology to produce bioengineered RNA agents(BERA), namely BERA/miR-27b-3p and BERA/miR-328-3p, via fermentation. When introduced into human cells, BERA/miR-27b-3p and BERA/miR-328-3p were selectively processed to target miRNAs and thus knock down CYP3A4 and ABCG2 mRNA and their protein levels,respectively, as compared to cells treated with vehicle or control RNA. Consequently, BERA/miR-27b-3p led to a lower midazolam 10-hydroxylase activity, indicating the reduction of CYP3A4 activity. Likewise,BERA/miR-328-3p treatment elevated the intracellular accumulation of anticancer drug mitoxantrone, a classic substrate of ABCG2, hence sensitized the cells to chemotherapy. The results indicate that biologic miRNA agents made by RNA biotechnology may be applied to research on miRNA functions in the regulation of drug metabolism and disposition that could provide insights into the development of more effective therapies. | Xin Li Ye Tian Mei-Juan Tu Pui Yan Ho Neelu Batra Ai-Ming Yu | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 10 |
| 2 | Direct aerobic oxidation of monoalcohol and diols to acetals using tandem Ru@MOF catalysts显示文摘The aerobic oxidation of monoalcohols and diols to acetals is an important academic and industrial challenge for the production of fine chemicals and intermediates.The existing methods usually rely on a two-step process in which alcohols are first oxidized to aldehydes over metal catalysts(Ru,Pt,Pd)and then acetalized using acids.Due to the instability of aldehydes,how to avoid over-oxidation to their respective carboxylic acids and esters is a long-standing challenge.For this reason,certain non-conjugated dialdehydes have never been successfully produced from diol oxidation.Hereby we report a Ru@metal-organic framework(MOF)tandem catalyst containing ultra-fine Ru nanoparticles(<2 nm)for direct alcohol to acetal conversion of monoalcohol and diols with noformation of carboxylic acids.Mechanistic study reveals that the presence of Lewis acid sites in the MOF work in concert with Ru active sites to promptly convert aldehydes to acetals thereby effectively suppressing the formation of over-oxidation byproducts. | Songwei Zhang Jerry Pui Ho Li Jingpeng Zhao Dan Wu Biao Yuan Willinton Yesid Hernandez Wen-Juan Zhou Tao He Yi Yu Yong Yang Vitaly Ordomsky Tao Li | 2021 | Nano Research2021，14，2： | 2 |
| 3 | High performance spectral-phase surface plasmon resonance biosensors based on single anddouble-layer schemes 显示文摘 | Wang Cheng Ho Ho Pui Shum Ping | 2013 | Optics Communications2013，291，13： | 1 |
| 4 | Genomics-driven derivatization of the bioactive fungal sesterterpenoid variecolin:Creation of an unnatural analogue with improved anticancer properties显示文摘A biosynthetic gene cluster for the bioactive fungal sesterterpenoids variecolin(1)and variecolactone(2)was identified in Aspergillus aculeatus ATCC 16872.Heterologous production of 1 and 2 was achieved in Aspergillus oryzae by expressing the sesterterpene synthase VrcA and the cytochrome P450 VrcB.Intriguingly,the replacement of VrcB with homologous P450s from other fungal terpenoid pathways yielded three new variecolin analogues(5-7).Analysis of the compounds’anticancer activity in vitro and in vivo revealed that although 5 and 1 had comparable activities,5 was associated with significantly reduced toxic side effects in cancer-bearing mice,indicating its potentially broader therapeutic window.Our study describes the first tests of variecolin and its analogues in animals and demonstrates the utility of synthetic biology for creating molecules with improved biological activities. | Dexiu Yan Jemma Arakelyan Teng Wan Ritvik Raina Tsz Ki Chan Dohyun Ahn Vladimir Kushnarev Tsz Kiu Cheung Ho Ching Chan Inseo Choi Pui Yi Ho Feijun Hu Yujeong Kim Hill Lam Lau Ying Lo Law Chi Seng Leung Chun Yin Tong Kai Kap Wong Wing Lam Yim Nikolay S.Karnaukhov Richard Y.C.Kong Maria V.Babak Yudai Matsuda | 2024 | Acta Pharmaceutica Sinica B2024，14，1： | 0 |
| 5 | Long-Term and Immediate Impacts of Health Emergency and Disaster Risk Management(Health-EDRM) Education Interventions in a Rural Chinese Earthquake-Prone Transitional Village显示文摘Ma'an Qiao Village, a Dai and Yi ethnic minority-based community in Sichuan Province, China sustained complete infrastructure devastation during the 2008 Panzhihua earthquake. Health emergency and disaster risk management(Health-EDRM) education intervention programs were implemented in 2010 and 2011. This serial cross-sectional survey study aimed to examine the immediate and long-term impacts of the Health-EDRM interventions in this remote rural community. The findings demonstrate knowledge improvement in areas of water and sanitation, food and nutrition, and disaster preparedness immediately after the Health-EDRM education interventions. Temporal stability of knowledge retention was observed in household hygiene and waste management and smoking beliefs in 2018, 7 years after the interventions.Other important findings include knowledge uptake pattern differences of oral rehydration solution(ORS) between earthquake-prone and flood-prone communities. Usage of Internet and mobile technology for accessing disaster-related information was found to be independent of gender and income. Overall, this study demonstrated the knowledge improvement through Health-EDRM education interventions in a remote rural community. Promoting behavioral changes through interventions to raise awareness has the potential to reduce health risks in transitional post-disaster settings. Future programs should aim to identify evidence-based practices and explore how technology can support Health-EDRM education among vulnerable subgroups. | Emily Ying Yang Chan Janice Ying-en Ho Zhe Huang Jean Hee Kim Holly Ching Yu Lam Phoebe Pui Wun Chung Carol Ka Po Wong Sida Liu Sharon Chow | 2018 | International Journal of Disaster Risk Science2018，9，3： | 0 |
| 6 | Cardiac implantable therapeutic medical devices:A narrative review显示文摘Heart diseases are common life-threatening acute diseases.They are leading causes of mortality worldwide,especially significant in developed countries.Other than medications for therapies and prophylaxis,special treatment considerations with implantable cardiac devices are important to reduce mortality and medical disability.This paper aims to review indications,contraindications,efficacy,complications,and generic considerations of several commonly implanted cardiac devices including pacemakers,cardiac resynchronization devices,implantable cardiac defibrillators,left atrial appendage occlusion watchman devices,and ventricular assist devices.As various implantable therapeutic cardiac devices are sometimes carried in the bodies of patients with cardiac disease,practitioners of various specialties should be familiar with different cardiac devices on the management of different cardiac conditions while providing holistic care. | Christine Pui Sum Ho Sunny Chi Lik Au | 2021 | Journal of Acute Disease2021，10，3： | 0 |
| 7 | Traditional Chinese medicine poisoning in the emergency departments in Hong Kong: Trend, clinical presentation and predictors for poor outcome显示文摘Dear editor,Traditional Chinese medicine (TCM) is popular in many countries,especially in the Chinese population.^([1,2]) In Hong Kong,it has been estimated that around 15%of the population consumed Chinese medicine products/herbs in the preceding month.Common reasons for consumption include regulating bodily functions,curing disease,and building up health. | Rex Pui Kin Lam Eric Ho Yin Lau Wai Lam Yip Joe Kai Shing Leung Matthew Sik Hon Tsui | 2021 | World Journal of Emergency Medicine2021，12，2： | 0 |
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