|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Experimental investigation of a novel multi-effect solar desalination system based on humidification- dehumidification process 显示文摘 | Chang Zehui Zheng Hongfei Yang Yingjun | 2014 | Renewalbe Energy2014，，： | 1 |
| 2 | Regulatory role of sorting nexin 5 in protein stability and vesicular targeting of vesicular acetylcholine transporter to synaptic vesicle-like vesicles in PC12 cells显示文摘Accurate targeting of vesicular acetylcholine transporter(VAChT)to synaptic vesicles(SVs)is indispensable for efficient cholinergic transmission.Previous studies have suggested that the dileucine motif within the C-terminus of the transporter is sufficient for its targeting to SVs.However,the cytosolic machinery underlying specific regulation of VAChT trafficking and targeting to SVs is still unclear.Here we used the C-terminus of VAChT as a bait in a yeast two-hybrid screen to identify sorting nexin 5(SNX5)as its novel interacting protein.SNX5 was detected in the SVs enriched LP2 subcellular fraction of rat brain homogenate and showed strong colocalization with VAChT in both brain sections and PC12 cells.Binding assays suggested that the C-terminal domain of VAChT can interact with both BAR and PX domain of SNX5.Depletion of SNX5 enhanced the degradation of VAChT and the process was mediated through the lysosomal pathway.More importantly,we found that,in PC12 cells,the depletion of SNX5 expression significantly decreased the synaptic vesicle-like vesicles(SVLVs)localization of VAChT.Therefore,the results suggest that SNX5 is a novel regulator for both stability and SV targeting of VAChT. | Meihen Sun Xu Han Fei Chang Hongfei Xu Lesley Colgan Yongjian Liu | 2021 | The Journal of Biomedical Research2021，35，5： | 1 |
| 3 | Experimental investigation and performance analysis on a group of multi-effect tubular solar desalination devices 显示文摘 | Zheng Hongfei Chang Zehui Chen Zhili | 2013 | Desalination2013，311，2： | 1 |
| 4 | Distinctive roles of Rac1 and Rab29 in LRRK2 mediated membrane trafficking and neurite outgrowth显示文摘Parkinson's disease(PD) associated leucine-rich repeat kinase 2(LRRK2) mutants have shown pathogenic effects on a variety of subcellular processes. Two small GTPases Rac1 and Rab29 have been indicated as possible downstream effectors participating in LRRK2 signaling but their detail mechanisms remain unclear. In this study, we have used biochemical and cell biology approaches to address whether two GTPases interact with LRRK2 and hence function differently in LRRK2 mediated pathogenesis. Here we show that Rac1 and Rab29 specifically interact with LRRK2 with higher affinity for Rab29 and with different preference in functional domain binding. Mutant Rab29 but not Racl alters the endosome-to-TGN retrograde trafficking of a cargo protein cation-independent mannose-6-phosphate receptor(CI-M6 PR) and its stability. On the other hand, overexpressed wild type Rab29 but not Racl rescued the altered retrograde membrane trafficking induced by the pathogenic mutant LRRK2^(G2019 S). Furthermore,both Rac1 and Rab29 rescued neurite shortening in differentiated SH-SY5 Y cells induced by LRRK2^(G2019 S). Our study strongly suggests that Rac1 and Rab29 are involved in distinct functions as downstream effectors in LRRK2 signaling pathways. | Min Feng Xin Hu Na Li Fan Hu Fei Chang Hongfei Xu Yongjian Liu | 2018 | The Journal of Biomedical Research2018，32，2： | 1 |
| 5 | Luminal/extracellular domains of chimeric CI-M6PR-C proteins interfere with their retrograde endosome-to-TGN trafficking in the transient expression system显示文摘The membrane trafficking of cation-independent mannose 6-phosphate receptor(CI-M6PR) between the transGolgi network(TGN) and endosomal compartments is not only critical for maintaining lysosomal function but also a well-known event for understanding molecular and cellular mechanisms in retrograde endosome-to-TGN trafficking.Although it has been well established in literature that the C-terminus of bovine CI-M6PR determines its retrograde trafficking,it remains unclear whether the luminal domain of the protein plays a role on these sorting events.In this study,we found that partial deletion of luminal domain of human CI-M6PR mistargeted the mutant protein to nonTGN compartments.Moreover,replacing the luminal domain of both bovine and human CI-M6PR with that from irrelevant membrane proteins such as CD8 or Tac also altered the TGN targeting of the chimeric proteins.On the other hand,only short sequence from HA fused with the transmembrane domain and C-terminus of the receptor,HA-hCIM6PR-tail,resulted in its preferential targeting to TGN as for the full length receptor,strongly suggesting that sorting of the receptor may be influenced by luminal sequence.Furthermore,using this luminal truncated form of HA-hCIM6 PR as a model cargo,we found that the trafficking of the chimeric protein was regulated by the retromer complex through interacting with SNX5.In conclusion,our study strongly suggested that the disrupted luminal domain from hCI-M6PR or other irrelevant membrane proteins interfere with the process of membrane trafficking and TGN targeting of CI-M6PR. | Fei Chang Na Li Kang Yan Yumin Huang Hongfei Xu Yongjian Liu | 2018 | The Journal of Biomedical Research2018，32，4： | 1 |
| 6 | Retrograde trafficking of VMAT2 and its role in protein stability in non-neuronal cells显示文摘Increasing evidence suggests that the impaired neuroprotection of vesicular monoamine transporter 2(VMAT2)contributes to the pathogenesis of Parkinson's disease.That has been linked to aberrant subcellular retrograde trafficking as strongly indicated by recent genomic studies on familial Parkinson's diseases.However,whether VMAT2 function is regulated by retrograde trafficking is unknown.By using biochemistry and cell biology approaches,we have shown that VMAT2 was stringently localized to the trans-Golgi network and underwent retrograde trafficking in non-neuronal cells.The transporter also interacted with the key component of retromer,Vps35,biochemically and subcellularly.Using specific siRNA,we further showed that Vps35 depletion altered subcellular localization of VMAT2.Moreover,siRNA-mediated Vps35 knockdown also decreased the stability of VMAT2 as demonstrated by the reduced half-life.Thus,our work suggested that altered vesicular trafficking of VMAT2 may play a vital role in neuroprotection of the transporter as well as in the pathogenesis of Parkinson's disease. | Qiuzi Wu Hongfei Xu Wei Wang Fei Chang Yu Jiang Yongjian Liu | 2016 | The Journal of Biomedical Research2016，30，6： | 1 |
| 7 | RUP2 facilitates UVR8 redimerization via two interfaces显示文摘The plant UV-B photoreceptor UV RESISTANCE LOCUS 8(UVR8)exists as a homodimer in its inactive ground state.Upon UV-B exposure,UVR8monomerizes and interacts with a downstreamkey regulator,theCONSTITUTIVE PHOTOMORPHOGENIC 1/SUPPRESSOR OF PHYA(COP1/SPA)E3 ubiquitin ligase complex,to initiate UV-B signaling.Two WD40 proteins,REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1(RUP1)and RUP2 directly interact with monomeric UVR8 and facilitate UVR8 ground state reversion,completing the UVR8 photocycle.Here,we reconstituted the RUP-mediated UVR8 redimerization process in vitro and reported the structure of the RUP2-UVR8^(W285A) complex(2.0A).RUP2 and UVR8^(W285A) formed a heterodimer via two distinct interfaces,designated Interface 1 and 2.The previously characterized Interface 1 is found between the RUP2 WD40 domain and the UVR8 C27 subregion.The newly identified Interface 2 is formed through interactions between the RUP2 WD40 domain and the UVR8 core domain.Disruption of Interface 2 impairedUV-B induced photomorphogenic development in Arabidopsis thaliana.Further biochemical analysis indicated that both interfaces are important for RUP2-UVR8 interactions and RUP2-mediated facilitation of UVR8 redimerization.Our findings suggest that the two-interface-interaction mode is adopted by both RUP2 and COP1 when they interact with UVR8,marking a step forward in understanding the molecular basis that underpins the interactions between UVR8 and its photocycle regulators. | Lixia Wang Yidong Wang Hongfei Chang Hui Ren Xinquan Wu Jia Wen Zeyuan Guan Ling Ma Liang Qiu Junjie Yan Delin Zhang Xi Huang Ping Yin | 2023 | Plant Communications2023，4，1： | 0 |
| 8 | The bright star survey telescope for the planetary transit survey in Antarctica显示文摘Transiting extrasolar planets(exoplanets),especially those orbiting bright stars, are desired for study of the diversity of planetary compositions, internal structures and atmospheres beyond our solar system. Dome A at Antarctica is a promising site for planetary transit surveys,where the continuous darkness and the large clear-sky fraction in the winter months greatly enhance the detection efficiency. The Chinese Small Telescope ARray and the Antarctic Survey Telescopes are the first facilities that have been operated at Dome A for use in exoplanet surveys. To increase the sky coverage, a low-temperature-resistant wide-field robotic telescope, named the bright star survey telescope(BSST), has been developed to join the ongoing planetary transit survey in Antarctica. The BSST has an aperture size of 300 mm and is equipped with a largeframe 4K×4K CCD camera to receive starlight from a 3.°4×3.°4 field of view. The BSST was operated at Lijiang observatory in April and May 2015 for a test run.Photometric precision of 3.5 mmag was achieved for stars with V~11 mag using 75 s exposures. The transiting events of two Jupiter-size exoplanets, HAT-P-3b and HATP-12 b, were observed on May 10 and May 20, 2015,respectively. | Qiguo Tian Peng Jiang Fujia Du Jian Wang Zhengyang Li Xiaoyan Li Zhiyong Zhang Haiping Lu Xiangyan Yuan Huigen Liu Hui Zhang Luming Sun Liang Chang Jianguo Wang Shaohua Zhang Tuo Ji Xiheng Shi Jie Chen Guangyu Zhang Minghao Jia Jiajing Liu Junyan Zhou Xiang Pan Shucheng Dong Fengxin Jiang Hongfei Zhang Jilin Zhou Lifan Wang Hongyan Zhou | 2016 | Science Bulletin2016，61，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员