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| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | The measurement of chronic pain and health-related quality of life following inguinal hernia repair: a review of the literature 显示文摘 | van Hanswijck JP Lloyd A Horsfall L | 2008 | Hernia2008，12，6： | 1 |
| 3 | Ser- um Bilirubin and Risk of Respiratory Disease and Death显示文摘 | HORSFALL L J RAIT G WALTERS K | 2011 | JaMa2011，，： | 1 |
| 4 | Effect of bioaugmentation on the performance of activated sludge reactors 显示文摘 | Hung Y T Shah D B Horsfall F L | 1987 | ProcessBiochemistry1987，，7： | 1 |
| 5 | Prediagnostic presentations of Parkinson' s disease in primary care: a case- control study显示文摘 | Schrag A Horsfall L Waiters K | 2015 | Lancet Neurol2015，14，1： | 1 |
| 6 | Persistent antigenic variation of influenza aviruses after incomplete neutralization in ovo with heterologous immune serum 显示文摘 | Archetti I Horsfall F L | 1950 | J Exp Med1950，92，： | 1 |
| 7 | Persistent antigenic variationof influenza A viruses after incomplete neutralization inova with heterologous immune serum显示文摘 | Archetti I Horsfall F L | 1950 | J ExptlMed1950，92，： | 1 |
| 8 | Medication beliefs predict medication adherence in ambulatory patients with decompensated cirrhosis显示文摘AIM To investigate the impact of medication beliefs, illness perceptions and quality of life on medication adherence in people with decompensated cirrhosis.METHODS One hundred adults with decompensated cirrhosis completed a structured questionnaire when they attended for routine outpatient hepatology review. Measures of self-reported medication adherence(Morisky Medication Adherence Scale), beliefs surrounding medications(Beliefs about Medicines Questionnaire), perceptions of illness and medicines(Brief Illness Perception Questionnaire), and quality of life(Chronic Liver Disease Questionnaire) were examined. Clinical data were obtained via patient history and review of medical records. Least absolute shrinkage and selection operator and stepwise backwards regression techniques were used to construct the multivariable logistic regression model. Statistical significance was set at alpha = 0.05.RESULTS Medication adherence was ' High ' in 42 % o f participants, 'Medium' in 37%, and 'Low' in 21%. Compared to patients with 'High' adherence, those with 'Medium' or 'Low' adherence were more likely to report difficulty affording their medications(P < 0.001), lower perception of treatment helpfulness(P = 0.003) and stronger medication concerns relative to medication necessity beliefs(P = 0.003). People with 'Low' adherence also experienced greater symptom burden and poorer quality of life, including more frequent abdominal pain(P = 0.023), shortness of breath(P = 0.030), and emotional disturbances(P = 0.050). Multivariable analysis identified having stronger medication concerns relative to necessity beliefs(Necessity-Concerns Differential ≤ 5, OR = 3.66, 95%CI: 1.18-11.40) and more frequent shortness of breath(shortness of breath score ≤ 3, OR = 3.87,95%CI: 1.22-12.25) as independent predictors of 'Low'adherence.CONCLUSION The association between 'Low' adherence and patients having strong concerns or doubting the necessity or helpfulness of their medications should be explored further given the clinical relevance. | Kelly L Hayward Patricia C Valery Jennifer H Martin Antara Karmakar Preya J Patel Leigh U Horsfall Caroline J Tallis Katherine A Stuart Penny L Wright David D Smith Katharine M Irvine Elizabeth E Powell W Neil Cottrell | 2017 | World Journal of Gastroenterology2017，23，40： | 1 |
| 9 | Heavy metal contaminants and processing effects on the composition,storage stability and fatty acid profiles of five common commercially available fish species in Oron Local Government,Nigeria显示文摘 | Eboh L Horsfall D M Mayo B E | 2006 | Food Chemistry2006，，97： | 1 |
| 10 | Sorption of cadmium ( i ) and zinc ( I )ions from aqueous solutions by cassava waste biomass显示文摘 | M Horsfal L Jr A A Abia | 2003 | Water research2003，37，： | 1 |
| 11 | Prediagnostic pre- sentations of Parkinson' s disease in primary care: a case- control study显示文摘 | Schrag A Horsfall L Waiters K | 2015 | Lancet Neurol2015，14，1： | 1 |
| 12 | Persistent antigenic variation of influen- za A viruses after incomplete neutralization in ovo with heterolo- gous immune serum 显示文摘 | Archetti I Horsfall F L | 1950 | J Exp Med1950，92，5： | 1 |
| 13 | Prediaguostie presentations of Parkinson' s disease in primary care: a ease- control study显示文摘 | Sehrag A Horsfall L Waiters K | 2015 | Lancet Neurol2015，14，1： | 1 |
| 14 | Prediagnostic pre- sentations of Parkinson's disease in primary care: a case- control study显示文摘 | Schrag A Horsfall L Waiters K | 2015 | Lancet Neurol2015，14，1： | 1 |
| 15 | Baceria Augmentation of Wastewater Treatment显示文摘 | HORSFALL F L | 1979 | Journal of the New England WCPA1979，13，2： | 1 |
| 16 | Prediaguustic presentations of Parkinson' s disease in prima care: a ease- control study 显示文摘 | Schrag A Horsfall L Walters K | 2015 | Lancet Neurol2015，14，1： | 1 |
| 17 | Serum bilirubin and risk of respirator disease and death显示文摘 | Horsfall L J Rait G Walters K | 2011 | JAMA2011，305，7： | 1 |
| 18 | Prediagnostic presentations of Parkinson's disease in primary care: a case-control study显示文摘 | Schrag A Horsfall L Waiters K | 2015 | Lancet Neurol2015，14，1： | 1 |
| 19 | Persistent antigenic variation of influenza a viruses after incomplete neutralization in vivo with heterologous immune serum 显示文摘 | ARCHETTI I HORSFALL F L JR | 1950 | J Exp Med1950，92，： | 1 |
| 20 | Four Recent Influenza Epidemics: an Experimental Study显示文摘 | Horsfall F L Hahn R G Rickard E R | 1940 | The Journal of clinical investigation1940，19，： | 1 |
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