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| 1 | The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp.chinensis Makino)显示文摘Non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) is one of the most important vegetables in eastern China.A genetic linkage map was constructed using 127 doubled haploid(DH) lines,and the DH population was derived from a commercial hybrid 'Hanxiao'(lines SW-13 × L-118).Out of the 614 polymorphic markers,43.49% were not assigned to any of the linkage groups(LGs).Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios,and the direction of distorted segregation was mainly toward the paternal parent L-118.After sequentially removing the markers that had an interval distance smaller than 1 cM from the upper marker,the overall quality of the linkage map was increased.Two hundred and sixty-eight molecular markers were mapped into 10 LGs,which were anchored to the corresponding chromosome of the B.rapa reference map based on common simple sequence repeat(SSR) markers.The map covers 973.38 cM of the genome and the average interval distance between markers was 3.63 cM.The number of markers on each LG ranged from 18(R08) to 64(R07),with an average interval distance within a single LG from 1.70 cM(R07) to 6.71 cM(R06).Among these mapped markers,169 were sequence-related amplified polymorphism(SRAP) mo-lecular markers,50 were SSR markers and 49 were random amplification polymorphic DNA(RAPD) markers.With further saturation to the LG,the current map offers a genetic tool for loci analysis for important agronomic traits. | Yan Cheng Jianfeng Geng Jingyi Zhang Qian Wang Qingyu Ban Xilin Hou | 2009 | Journal of Genetics and Genomics2009，36，8： | 9 |
| 2 | Divergent evolutionary patterns of the MAPK cascade genes in Brassica rapa and plant phylogenetics显示文摘Mitogen-activated protein kinase(MAPK)cascade signal transduction modules play crucial roles in regulating many biological processes in plants.These cascades are composed of three classes of hierarchically organized protein kinases,MAPKKKs,MAPKKs and MAPKs.Here,we analyzed gene retention,phylogenetic,evolution and expression patterns of MAPK cascade genes in Brassica rapa.We further found that the MAPK branches,classes III and IV,appeared after the split of bryophytes and green algae after analyzing the MAPK cascade genes in 8 species,and their rapid expansion led to the great size of the families of MAPKs.In contrast,the ancestral class I subfamily of MAPKK gene families have been highly conserved from algae to angiosperms.For the MAPKKK family,the MEKK and Raf subfamily share a common evolutionary origin,and Raf plays a major role in the expansion of the MAPKKK gene family.The cis-elements and interaction network analyses showed the important function of MAPK cascade genes in development and stress responses in B.rapa.This study provides a solid foundation for molecular evolution analyses of MAPK cascade genes. | Peng Wu Wenli Wang Ying Li Xilin Hou | 2017 | Horticulture Research2017，4，1： | 6 |
| 3 | Job Satisfaction: Linking Perceived Organizational Support, Organizational Justice with Work Outcomes in China显示文摘这研究发展并且测试在中国上下文检验工作满足的调停的角色的一个完整的调停模型,基于在三家小、中等的大小的企业的 424 个雇员的调查。数据分析出现一好与完整的调停和前身的所有四个班合适即,察觉的组织上的支持,程序,分发,并且 interactional 公正。特别地,程序的公正贡献满足的预言。工作满足也被显示最调停 antecedentconsequence 关系,除了二在察觉的组织上的支持 POSturnover 和程序的 justiceconsequences 之间。而且,仅仅有四个直接连接,包括到针对个人的公民资格行为的 POS,分发的公正到周转意愿, interactional 公正到针对组织和周转的公民资格行为。这些直接连接建议工作满足充分不调停关系。 | Rentao Miao Jianmin Sun Xilin Hou Tianzhu Li | 2012 | Frontiers of Business Research in China2012，6，2： | 4 |
| 4 | In tegrative iTRAQ-based proteomic and transcriptomic analysis reveals the accumulation patter ns of key metabolites associated with oil quality during seed ripening of Camellia oleifera显示文摘Camellia oleifera(C.oleifera)is one of the four major woody oil-bearing crops in the world and has relatively high ecological,economic,and medicinal value.Its seeds undergo a series of complex physiological and biochemical changes during ripening,which is mainly manifested as the accumulation and transformation of certain metabolites closely related to oil quality,especially flavonoids and fatty acids.To obtain new insights into the underlying molecular mechanisms,a parallel analysis of the transcriptome and proteome profiles of C.oleifera seeds at different maturity levels was conducted using RNA sequencing(RNA-seq)and isobaric tags for relative and absolute quantification(iTRAQ)complemented with gas chromatography-mass spectrometry(GC-MS)data.A total of 16,530 transcripts and 1228 proteins were recognized with significant differential abundances in pairwise comparisons of samples at various developmental stages.Among these,317 were coexpressed with a poor correlation,and most were involved in metabolic processes,including fatty acid metabolism,a-linolenic acid metabolism,and glutathione metabolism.In addition,the content of total flavonoids decreased gradually with seed maturity,and the levels of fatty acids generally peaked at the fat accumulation stage;these results basically agreed with the regulation patterns of genes or proteins in the corresponding pathways.The expression levels of proteins annotated as upstream candidates of phenylalanine ammonia-lyase(PAL)and chalcone synthase(CHS)as well as their cognate transcripts were positively correlated with the variation in the flavonoid content,while shikimate O-hydroxycinnamoyltransferase(HCT)-(encoding genes had the opposite pattern.The increase in the abundance of proteins and mRNAs corresponding to alcohol dehydrogenase(ADH)was associated with a reduction in linoleic acid synthesis.Using weighted gene coexpression network analysis(WGCNA),we further identi fied six unique modules related to flavonoid,oil,and fatty acid anabolism that contained hub genes or proteins similar to transcription factors(TFs),such as MADS intervening keratin-like and C-terminal(MIKC_MAD s),type-B authentic response regulator(ARR-B),and basic helix-loop-helix(bH〔H).Finally,based on the known metabolic pathways and WGCNA combined with the correlation analysis,fi ve coexpressed transcripts and proteins composed of cinnamyl-alcohol dehyd rogenases(CADs),caffeicacid 3-O-methyltransferase(COMT),fl avonol synthase(FLS),and 4-coumarate:CoA ligaas(4CL)were screened out.With this exploratory multiomics dataset,our results presented a dynamic picture regarding the maturation process of C.oleifera seeds on Hainan Island,not only revealing the temporal specific expression of key candidate genes and proteins but also providing a scientific basis for the genetic improvement of this tree species. | Zhouchen Ye Jing Yu Wuping Yan Junfeng Zhang Dongmei Yang Guanglong Yao Zijin Liu Yougen Wu Xilin Hou | 2021 | Horticulture Research2021，8，1： | 3 |
| 5 | A Universal Principle to Accurately Synthesize Atomically Dispersed Metal–N_4 Sites for CO_2 Electroreduction显示文摘Atomically dispersed metal-nitrogen sites-anchored carbon materials have been developed as effective catalysts for CO2 electroreduction(CO2 ER),but they still suffer from the imprecisely control of type and coordination number of N atoms bonded with central metal.Herein,we develop a family of single metal atom bonded by N atoms anchored on carbons(SAs-M-N-C,M=Fe,Co,Ni,Cu)for CO2 ER,which composed of accurate pyrrole-type M-N4 structures with isolated metal atom coordinated by four pyrrolic N atoms.Benefitting from atomically coordinated environment and specific selectivity of M-N4 centers,SAs-Ni-N-C exhibits superior CO2 ER performance with onset potential of-0.3 V,CO Faradaic efficiency(F.E.) of 98.5%at-0.7 V,along with low Tafel slope of 115 mV dec-1 and superior stability of 50 h,exceeding all the previously reported M-N-C electrocatalysts for CO2-to-CO conversion.Experimental results manifest that the different intrinsic activities of M-N4 structures in SAs-M-N-C result in the corresponding sequence of Ni> Fe> Cu> Co for CO2 ER performance.An integrated Zn-CO2 battery with Zn foil and SAs-Ni-N-C is constructed to simultaneously achieve CO2-to-CO conversion and electric energy output,which delivers a peak power density of 1.4 mW cm-2 and maximum CO F.E.of 93.3%. | Wanzhen Zheng Feng Chen Qi Zeng Zhongjian Li Bin Yang Lecheng Lei Qinghua Zhang Feng He Xilin Wu Yang Hou | 2020 | Nano-Micro Letters2020，12，9： | 2 |
| 6 | Active oxygen metabolism in the floral buds and leaves of the new cytoplasm male sterile (CMS) line and its maintainer line of non heading Chinese cabbage显示文摘 | Yuan Jianyu Hou Xilin Zhang Changwei | 2007 | Front Agric China2007，1，1： | 1 |
| 7 | Response of antioxidant activity to excess copper in two cultivars of Brassica campestris ssp. chinensis Makino显示文摘 | Ying Li Yuping Song Gongjun Shi Jianjun Wang Xilin Hou | 2009 | Acta Physiologiae Plantarum2009，，1： | 1 |
| 8 | Induction of Anthurium ondraeanum'Arizona'tetraploid by colchicine in vitro显示文摘 | Chen Chao Hou Xilin Zhang Hongxin | 2011 | Euphytica2011，181，： | 1 |
| 9 | Cloning and characterization of the BcTuR3 gene related to resistance to Turnip Mosaic Virus (TuMV) from non-heading Chinese cabbage显示文摘 | Ma Jingfan Hou Xilin Xiao Dong | 2010 | Plant Mol Biol Rep2010，28，： | 1 |
| 10 | Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots显示文摘Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin.The one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding. | Huiyu Wang Yushan Zheng Qian Zhou Ying Li Tongkun Liu Xilin Hou | 2024 | Horticultural Plant Journal2024，10，2： | 0 |
| 11 | Genome-wide identification and analysis of cytokinin dehydrogenase/oxidase(CKX) family genes in Brassica oleracea L.reveals their involvement in response to Plasmodiophora brassicae infections显示文摘Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple pathogens.However,a comprehensive analysis of cytokinin dehydrogenase/oxidase(CKX)family genes in cabbage has not been reported.In this study,a total of 36 CKX genes were identified using a genome-wide search method.Phylogenetic analysis classified these genes into three groups.They were distributed unevenly across nine chromosomes in B.oleracea,and 15 of them did not contain any introns.The results of colinearity analysis showed that 36 CKX gene in Arabidopsis was present in several copies in the Brassica oleracea genome.An analysis of cisacting elements indicated that all genes possessed at least one stress or hormone responsive cis-acting element.A heatmap of CKX gene expression showed the patterns of expression of these genes in various tissues and organs.Three genes(Bol028363,Bol031036 and Bol018140)were relatively highly expressed in all of the investigated tissues under normal conditions,showing the expression profile of housekeeping genes.Generally,the expression patterns of CKX genes in Jingfeng 1 and Xiangan 336 were quite different under the same treatment.Notably,three genes(Bol020547,Bol028392 and Bol045724)were significantly down-regulated and up-regulated in the susceptible and resistant material,respectively,after inoculation,which may indicate their crucial roles in resistance to clubroot disease.The results provide insights for better understanding the roles of CKX genes in the B.oleracea–P.brassicae interaction. | Mingzhao Zhu Yong Wang Shujin Lu Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Zhiyuan Fang Xilin Hou | 2022 | Horticultural Plant Journal2022，8，1： | 0 |
| 12 | BcRISP1,isolated from non-heading Chinese cabbage,decreases the seed set of transgenic Arabidopsis显示文摘Mitochondria are the energy sources of plant cells and are involved in regulating cell development.Ubiquinol–cytochrome c reductase iron-sulfur protein,which is necessary for mitochondrial respiration,is a subunit of mitochondrial electron transport chain multimeric enzyme complexes.To better understand the biological function of the ubiquinol–cytochrome c reductase iron–sulfur protein,the full-length cDNA of BcRISP1 was cloned;it was found to contain 810 base pairs and encode 269 amino acids.Unusually,high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction(qRT-PCR)analysis of cytoplasmic male sterile lines and maintainer lines.The seed set was affected by the overexpression of BcRISP1,and shorter siliques with lower seed sets were observed in 35S::BcRISP1 Arabidopsis plants.These characteristics may have resulted from the reduced formation of pollen and impaired pollen tube growth.qRT-PCR results revealed that in 35S::BcRISP1 plants,the expression levels of the mitochondrial respiratory chain-related genes,COX10 and RIP1,were enhanced,whereas the expression levels of QCR7 and SDH2-1 were reduced.This result implies that overexpression of BcRISP1 in transgenic Arabidopsis plants may disrupt the mitochondrial electron transport chain by affecting the expression of mitochondrial respiratory chain-related genes and therefore,reducing the seed set. | Tongkun Liu Yu Qian Weike Duan Jun Ren Xilin Hou Ying Li | 2014 | Horticulture Research2014，1，1： | 0 |
| 13 | Transcriptome profiling reveals the association of multiple genes and pathways contributing to hormonal control in celery leaves显示文摘Celery is a vital vegetable belonging to the Apiaceae family.The leaves of celery are its main edible part with high nutritional value.Hormone signaling plays diverse and critical roles in controlling plant growth and development.However,the molecular mechanism of hormone regulating growth and development in celery leaves has not been investigated.Here,we aimed to understand the molecular functions of genes related to hormone metabolism in celery leaf growth and development.A total of 77 hormone-related differentially expressed genes(DEGs)were identified from the transcriptome of celery leaves at three development stages.The roles and interactions of DEGs in the growth and development of celery leaves were discussed.The contents of multiple hormones(IAA,ZR,ABA,BR,GA3,and MeJA)in celery leaf development were also detected.The changes of endogenous hormone level during the development of celery leaves could be regulated by the expressions of hormone-related genes.Our results indicated that the plant hormones had a complex regulatory mechanism for the growth of celery leaves.Our current findings will provide potential valuable references for the future research on celery leaf development. | Jiexia Liu Kai Feng Xilin Hou Hui Li Guanglong Wang Zhisheng Xu Aisheng Xiong | 2019 | Acta Biochimica et Biophysica Sinica2019，51，5： | 0 |
| 14 | BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage显示文摘Shoot branching is a decisive factor for crop yield.Molecular mechanism for regulating shoot branching(tillering)needs to be determined.Plenty of previous studies have illustrated that BRANCHED1(BRC1)is a key integrator of shoot branching regulating signals.However,BcBRC1 function in non-heading Chinese cabbage(Brassica campestris ssp.chinensis)(NHCC)remains unknown.Here,we defined two BRC1 orthologs,BcBRC1a and BcBRC1b,from NHCC and focused on the BcBRC1a gene to describe its alternative splicing characteristic and structure.BcBRC1a was expressed rhythmically and mainly in leaf axils at the'Maertou'cultivar tillering stage.BcBRC1aL encoded a nuclear location protein.Its ectopic expression caused Arabidopsis growth inhibition and silencing BcBRC1a led to increased tiller numbers in'Maertou'.Removing the shoot tips of NHCC caused axillary buds to be released from apical dominance and BcBRC1a expression down-regulation.Our research determined that BcBRC1a acts as a negative regulator for tillering in non-heading Chinese cabbage and sets the foundation for further studies. | Yan Long Tianzi Zhao Lanlan Xu Wei Zhang Feiyi Huang Jianjun Wang Xilin Hou Ying Li | 2022 | Vegetable Research2022，2，1： | 0 |
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