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| 1 | Effects of methotrexate on the developments of heart and vessel in zebrafish显示文摘Methotrexate (MTX ) , folic 酸的一个对手,能禁止在 tetrahydrofolic 酸和胚胎的开发的合成很重要的 dihydrofolate reductase (DHFR ) 。在这研究,我们在暴露于 1.5 以后发现了那 ? 在 610 个小时的公里 MTX 授精以后, zebrafish 胚胎没能形成正常心血管的系统。在对待 MTX 的胚胎,室和中庭的词法发展被破坏,心脏的扭曲是反常的,心率和室的弄短部分被减少,并且脉管的发展被破坏。我们也与钙盐五水合物(CF ) 能在对待 MTX 的胚胎在心和容器引起改进开发的 folinic 酸与 dhfrgfp mRNA 或处理发现那任何一个 microinjection,它证明那 MTX 由禁止 DHFR 导致了畸形性。象 hand2, mef2a, mef2c,和 flk-1 那样的基因的抄本层次在对待 MTX 的胚胎被减少。与对待 MTX 的组相比, hand2, mef2a, mef2c,和 flk-1 的抄本层次在 MTX + dhfrgfp 被增加注射 mRNA 的组并且在 MTX + CF 组。我们的结果显示在对待 MTX 的胚胎的心和容器的破坏发展与 hand2, mef2a, mef2c,和 flk-1 的减少的抄本层次有关。 | Shuna Sun Yonghao Gui Yuexiang Wang Linxi Qian Xuefei Liu Qiu Jiang Houyan Song | 2009 | Acta Biochimica et Biophysica Sinica2009，41，1： | 10 |
| 2 | Ethanol disrupts the formation of hypochord and dorsal aorta during the development of embryonic zebrafish显示文摘Exposure to ethanol during human embryonic period has severe teratogenic effects on the cardiovascular system. In our study, we demonstrated that ethanol of gradient concentra-tions can interfere with the establishment of circulatory system in embryonic zebrafish. The ef-fective concentration to cause 50% malformations (EC50) was 182.5 mmol/L. The ethanol pulse exposure experiment displayed that dome stage during embryogenesis is the sensitive time window to ethanol. It is found that 400 mmol/L ethanol pulse exposure can induce circulatory defects in 43% treated embryos. We ruled out the possibility that ethanol can interfere with the process of hematopoiesis in zebrafish. By employing in situ hybridization with endothelial bio-marker (Flk-1), we revealed that ethanol disrupts the establishment of trunk axial vasculature, but has no effect on cranial vessels. Combined with the results of semi-thin histological sections, the in situ hybridization experiments with arterial and venous biomarkers (ephrinB2, ephB4) sug-gested that ethanol mainly interrupts the development of dorsal aorta while has little effect on axial vein. Further study indicated the negative influence of ethanol on the development of hy-pochord in zebrafish. The consequent lack of vasculogenic factors including Radar and Ang-1 partly explains the defects in formation and integrity of dorsal aorta. These results provide im-portant clues to the study of adverse effects of ethanol on the cardiovascular development in human fetus. | QIAN Linxi WANG Yuexiang JIANG Qiu ZHONG Tao SONG Houyan | 2005 | Science China(Life Sciences)2005，48，6： | 6 |
| 3 | Dihydrofolate reductase is required for the development of heart and outflow tract in zebrafish显示文摘Folic 酸为抑制能在脊椎动物引起先天的心缺点的胚胎的开发和 folic 酸是很重要的。Dihydrofolate reductase (DHFR ) 是在叶酸调停新陈代谢的关键酶。DHFR 的机能障碍破坏酸参予 folic 的关键生物过程。DHFR 基因在脊椎动物进化期间被保存。在心脏的开发调查 DHFR 的角色是重要的。在这研究,我们显示出那 DHFR 击倒在早阶段导致了 zebrafish 胚胎的反常开发。在心和流出道的明显的畸形性(经常) 也在 DHFR 击倒的胚胎被观察。DHFR overexpression 在 DHFR 击倒的组救了反常显型。DHFR NKX2.5 的表达式上的击倒的有的否定影响(NK2 抄写因素相关 5 ) , MEF2C (myocyte 特定的 enhancer 因素 2C ) , TBX20 (T 盒子 20 ) ,并且 TBX1 (T 盒子 1 ) 它在心脏的开发过程期间是重要 transcriptional 因素,当 DHFR overexpression 有积极效果时。DHFR 为刺猬小径被要求。DHFR 击倒的引起的减少的房间增长和增加的 apoptosis,当它的 overexpression 支持了房间增长并且禁止了 apoptosis 时。一起拿,我们的学习建议 DHFR 由调整基因抄写并且影响房间增长和 apoptosis 在 zebrafish 在心并且经常的发展起关键作用。 | Shuna Sun Yonghao Gui Qiu Jiang Houyan Song | 2011 | Acta Biochimica et Biophysica Sinica2011，43，12： | 5 |
| 4 | Effect of ionic strength on adsorption of As(Ⅲ) and As(V) on variable charge soils显示文摘The study was to investigate the adsorption behavior of arsenite (As(III)) and arsenate (As(V)) on two variable charge soils, i.e., Haplic Acrisol and Rhodic Ferralsol at dierent ionic strengths and pH with batch methods. Results indicated that the amount of As(III) adsorbed by these two soils increased with increasing solution pH, whereas it decreased with increasing ionic strength under the acidic condition. This suggested that As(III) was mainly adsorbed on soil positive charge sites through electrostatic attraction under the acidic condition. Moreover, intersects of As(V) adsorption-pH curves at dierent ionic strengths (a characteristic pH) are obtained for both soils. It was noted that above this pH, the adsorption of As(V) was increased with increasing ionic strength, whereas below it the reverse trend was true. Precisely the intersect pH was 3.6 for Haplic Acrisol and 4.5 for Rhodic Ferralsol, which was near the values of PZSE (soil point of zero salt eect) of these soils. The eects of ionic strength and pH on arsenate adsorption by these soils were interpreted by the adsorption model. The results of zeta potential suggested that the potential in adsorption plane becomes less negative with increasing ionic strength above soil PZSE and decreases with increasing ionic strength below soil PZSE. These results further supported the hypothesis of the adsorption model that the potential in the adsorption plane changes with ionic strength with an opposite trend to surface charge of the soils. Therefore, the change of the potential in the adsorption plane was mainly responsible for the change of arsenate adsorption induced by ionic strength on variable charge soils. | XU Renkou WANG Yong TIWARI Diwakar WANG Houyan | 2009 | Journal of Environmental Sciences2009，21，7： | 5 |
| 5 | Expression and characterization of the first kunitz domain of human tissue factor pathway inhibitor-2显示文摘 | Desheng Kong Duan Ma Hao Bai Hongshen Guo Xu Cai Wei Mo Qiqun Tang Houyan Song | 2004 | Biochemical and Biophysical Research Communications2004，，4： | 1 |
| 6 | Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts显示文摘 | Ping Li Chang Tong Ruty Mehrian-Shai Li Jia Nancy Wu Youzhen Yan Robert E. Maxson Eric N. Schulze Houyan Song Chih-Lin Hsieh Martin F. Pera Qi-Long Ying | 2008 | Cell2008，，7： | 1 |
| 7 | Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts显示文摘 | Ping Li Chang Tong Ruty Mehrian-Shai Li Jia Nancy Wu Youzhen Yan Robert E. Maxson Eric N. Schulze Houyan Song Chih-Lin Hsieh Martin F. Pera Qi-Long Ying | 2008 | Cell2008，，7： | 1 |
| 8 | On the WTO's Self-protection Rules to Achieve Win-Win显示文摘 | LiLian HouYan LiuJun | 2005 | China Legal Science2005，，6： | 1 |
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