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| 1 | Hydrogen Sulfide Promotes Wheat Seed Germination and Alleviates Oxidative Damage against Copper Stress显示文摘With the enhancement of copper(Cu) stress,the germination percentage of wheat seeds decreased gradually. Pretreatment with sodium hydrosulfide(NaHS),hydrogen sulfide(H2S) donor alleviated the inhibitory effect of Cu stress in a dose-dependent manner; whereas little visible symptom was observed in germinating seeds and radicle tips cultured in NaHS solutions. It was verified that H2S or HS-rather than other sulfur-containing components derived from NaHS attribute to the potential role in promoting seed germination against Cu stress. Further studies showed that NaHS could promote amylase and esterase activities,reduce Cu-induced disturbance of plasma membrane integrity in the radicle tips,and sustain lower levels of malondialdehyde and H2O2 in germinating seeds. Furthermore,NaHS pretreatment increased activities of superoxide dismutase and catalase and decreased that of lipoxygenase,but showed no significant effect on ascorbate peroxidase. Alternatively,NaHS prevented uptake of Cu and promoted the accumulation of free amino acids in seeds exposed to Cu. In addition,a rapid accumulation of endogenous H2S in seeds was observed at the early stage of germination,and higher level of H2S in NaHS-pretreated seeds. These data indicated that H2S was involved in the mechanism of germinating seeds' responses to Cu stress. | Hua Zhang Lan-Ying Hu Kang-Di Hu Yun-Dong He Song-Hua Wang Jian-Ping Luo | 2008 | Journal of Integrative Plant Biology2008，50，12： | 93 |
| 2 | Etiology and antimicrobial resistance of community-acquired pneumonia in adult patients in China显示文摘 | TAO Li-li HU Bi-jie HE Li-xian WEI Li XIE Hong-mei WANG Bao-qing LI Hua-ying CHEN Xue-hua ZHOU Chun-mei DENG Wei-wu | 2012 | Chinese Medical Journal2012，，17： | 91 |
| 3 | Gasdermin D is an executor of pyroptosis and required for interleukin-lp secretion显示文摘 | Wan-ting He Haoqiang Wan Lichen Hu Pengda Chen Xin Wang Zhe Huang Zhang-Hua Yang Chuan-Qi Zhong Jiahuai Han | 2015 | Cell Research2015，25，12： | 195 |
| 4 | Pyroptosis is driven by non-selective gasdermin-D pore and its morphology is different from MLKL channel-mediated necroptosis显示文摘Necroptosis 和 pyroptosis 是有血浆膜破裂的一个普通特征的规划房间死亡的二种形式。这里,我们与 necroptosis 比较学习了 pyroptosis 的形态学和机制。与 necroptosis 不同, pyroptosis 经历膜 blebbing 并且生产 apoptotic 在血浆膜以前的像身体的房间伸出(称为的 pyroptotic 身体) 破裂。在 necroptosis 的破裂是像爆炸的,而在 pyroptosis 它导致房间变平。necroptosis 的实行被混合的系 kinase 调停,这被知道像域(MLKL ) 在血浆膜的 oligomers,而 gasdermin-D (GSDMD ) 调停在它由 caspase-1 或 caspase-11 的劈开以后的 pyroptosis。我们显示出那 N 终端碎片 caspase 劈开产生的 GSDMD (GSDMD-N ) 也形成 oligomer 并且移居到血浆膜杀死房间。MLKL 和 GSDMD-N 是脂性的,两蛋白质的 N 终端序列为他们的 oligomerization 和血浆膜 translocation 是重要的。不同于在血浆膜上形成隧道的 MLKL,那导致渗透地胀大的选择离子的流入冲破的房间, GSDMD-N 形成非选择的毛孔并且不依靠增加的 osmolarity 破坏房间。我们的学习表明 GSDMD 的形成毛孔的活动和 MLKL 的形成隧道的活动在 pyroptosis 和 necroptosis 决定血浆膜破裂的不同方法。 | Xin Chen Wan-ting He Lichen Hu Jingxian Li Yuan Fang Xin Wang Xiaozheng Xu Zhuo Wang Kai Huang Jiahuai Han | 2016 | Cell Research2016，26，9： | 110 |
| 5 | SIMULATION OF MAXIMUM LIGHT USE EFFICIENCY FOR SOME TYPICAL VEGETATION TYPES IN CHINA显示文摘Maximum light use efficiency (εmax) is a key parameter for the estimation of net primary pro-ductivity (NPP) derived from remote sensing data. There are still many divergences about its value for each vegetation type. The εmax for some typical vegetation types in China is simulated using a modi-fied least squares function based on NOAA/AVHRR remote sensing data and field-observed NPP data. The vegetation classification accuracy is introduced to the process. The sensitivity analysis of εmax to vegetation classification accuracy is also conducted. The results show that the simulated values of εmax are greater than the value used in CASA model, and less than the values simulated with BIOME-BGC model. This is consistent with some other studies. The rela-tive error of εmax resulting from classification accuracy is ?5.5%―8.0%. This indicates that the simulated values of εmax are reliable and stable. | ZHU Wenquan PAN Yaozhong HE Hao YU Deyong HU Haibo | 2006 | Chinese Science Bulletin2006，51，4： | 61 |
| 6 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 7 | Tibetan Plateau climate dynamics: recent research progress and outlook显示文摘This paper reviews progress in the study of Tibetan Plateau(TP) climate dynamics over the past decade.Several theoretical frameworks, including thermal adaptation and the TP sensible heat(SH) driving air-pump, have been developed to identify the mechanisms responsible for the circulation anomaly produced by thermal forcing of the TP. Numerical simulations demonstrate that the thermal efects of large-scale orography, including the Tibetan and Iranian Plateaus(TIP), are crucial for the formation of the East Asian and South Asian summer monsoons(SASM) because the surface SH of the TIP is the major driver of the water vapor transport required for the genesis of the north branch of the SASM. he large-scale orography of the TP afects the Asian climate through thermal forcing in spring and summer, and mechanical forcing in winter. he TP forcing can also inluence the Asian summer monsoon(ASM) onset over the Bay of Bengal(BOB) by enhancing the BOB warm pool at the surface and by modulating the South Asian High(SAH) in the upper troposphere. On intra-seasonal timescales, the TP thermal forcing signiicantly modulates spring rainfall in southern China and generates the biweekly oscillation of the SAH in summer. Despite climate warming, the atmospheric heat source over the TP, particularly the spring SH,exhibits a clear weakening trend from the 1980 s to 2000 s. his weakening of the spring SH contributed to the anomalous ‘dry in the north' and ‘wet in the south' rainfall patern observed over East China. Also discussed are challenges to further understanding the mechanism of TP forcing on the multi-scale variability of the ASM. | Guoxiong Wu Anmin Duan Yimin Liu Jiangyu Mao Rongcai Ren Qing Bao Bian He Boqi Liu Wenting Hu | 2015 | National Science Review2015，2，1： | 51 |
| 8 | Treatment Efficiencies of Constructed Wetlands for Eutrophic Landscape River Water显示文摘The efficiencies of two types of constructed wetlands for the treatment of low-concentration polluted eutrophic land- scape river water were studied in the western section of the Qingyuan River at the Minhang campus of Shanghai Jiaotong University.The first wetland was a single-stage system using gravel as a filtration medium,and the second was a three- stage system filled with combinations of gravel,zeolite,and fly ash.Results from parallel operations of the wetlands showed that the three-stage constructed wetland could remove organics,nitrogen, and phosphorus successfully.At the same time,it could also decrease ammoniacal odour in the effluent.Compared to the single-stage constructed wetland,it had better nutrient removal efficiencies with a higher removal of 19.37%-65.27% for total phosphorus (TP) and 21.56%- 62.94% for total nitrogen (TN),respectively,during the operation period of 14 weeks.In terms of removal of chemical oxygen demand (COD), turbidity,and blue-green algae,these two wetland systems had equivalent performances.It was also found that in the western section of the test river,in which the two constructed wetlands were located, the water quality was much better than that in the eastern and middle sections without constructed wetland because COD,TN, and TP were all in a relatively lower level and the eutrophication could be prevented completely in the western section. | HE Sheng-Bing YAN Li KONG Hai-Nan LIU Zhi-Ming WU De-Yi HU Zhan-Bo | 2007 | Pedosphere2007，17，4： | 50 |
| 9 | Orbit determination and time synchronization for a GEO/IGSO satellite navigation constellation with regional tracking network显示文摘Aiming at regional services,the space segment of COMPASS (Phase I) satellite navigation system is a constellation of Geostationary Earth Orbit (GEO),Inclined Geostationary Earth Orbit (IGSO) and Medium Earth Orbit (MEO) satellites.Precise orbit determination (POD) for the satellites is limited by the geographic distribution of regional tracking stations.Independent time synchronization (TS) system is developed to supplement the regional tracking network,and satellite clock errors and orbit data may be obtained by simultaneously processing both tracking data and TS data.Consequently,inconsistency between tracking system and TS system caused by remaining instrumental errors not calibrated may decrease navigation accuracy.On the other hand,POD for the mixed constellation of GEO/IGSO/MEO with the regional tracking network leads to parameter estimations that are highly correlated.Notorious example of correlation is found between GEO's orbital elements and its clock errors.We estimate orbital elements and clock errors for a 3GEO+2IGSO constellation in this study using a multi-satellite precise orbit determination (MPOD) strategy,with which clock error elimination algorithm is applied to separate orbital and clock estimates to improve numerical efficiency.Satellite Laser Ranging (SLR) data are used to evaluate User Ranging Error (URE),which is the orbital error projected on a receiver's line-of-sight direction.Two-way radio-wave time transfer measurements are used to evaluate clock errors.Experimenting with data from the regional tracking network,we conclude that the fitting of code data is better than 1 m in terms of Root-Mean-Square (RMS),and fitting of carrier phase is better than 1 cm.For orbital evaluation,difference between computed receiver-satellite ranging based on estimated orbits and SLR measurements is better than 1 m (RMS).For clock estimates evaluation,2-hour linear-fitting shows that the satellite clock rates are about 1.E-10 s/s,while receiver clock rates are about 1×10 13-1×10 12 s/s.For the 72-hour POD experiment,the average differences between POD satellite clock rates estimates and clock measurements based on TS system are about 1×10 13 s/s,and for receiver clock rates,the differences are about 1×10 15 s/s. | ZHOU ShanShi HU XiaoGong WU Bin LIU Li QU WeiJing GUO Rui HE Feng CAO YueLing Wu XiaoLi ZHU LingFeng SHI Xin TAN HongLi | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，6： | 44 |
| 10 | A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice显示文摘A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice (Oryza sativa L.) varieties.In this study,we generated an F 2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 (PA64),an elite indica small-grain cultivar.Using QTL analysis,17 QTLs for five grain traits were detected on four different chromosomes.Eight of the QTLs were newly-identified in this study.In particular,qGL3-1,a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation,was fine-mapped to the 17 kb region of chromosome 3.A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL.A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution (C→A) at position 1092 in exon 10,resulting in replacement of Asp (D) in PA64 with Glu (E) in CW23 for the 364th amino acid.This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat.Genetic analysis of a near-isogenic line (NIL) for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect,and is suitable for use in molecular marker-assisted selection. | Zejun Hu Haohua He Shiyong Zhang Fan Sun Xiaoyun Xin Wenxiang Wang Xi Qian Jingshui Yang Xiaojin Luo | 2012 | Journal of Integrative Plant Biology2012，54，12： | 41 |
| 11 | A Novel QTL qTGW3 Encodes the GSK3/ SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice显示文摘谷物尺寸和形状在米饭是谷物重量和产量的重要决定因素。这里,我们报导一个新主要量的特点在米饭的地点(QTL ) , qTGW3,那种控制谷物尺寸和重量。这个地点, qTGW3,编码 OsSK41 (也作为 OsGSK5 知道) , GLYCOGEN SYNTHASE KINASE 3/SHAGGY-like 家庭的一个成员。带 OsSK41 的 loss-of-function 等位基因的瑞斯 near-isogenic 线增加了谷物长度和重量。我们证明那 OsSK41 交往与并且 phosphorylates 植物生长素反应因素 4 (OsARF4 ) 。有 OsARF4 的 OsSK41 的合作表示在米饭原物增加 OsARF4 的累积。OsARF4 的功能的损失导致更大的米饭谷物。定序 RNA 分析建议 OsARF4 和 OsSK41 镇压下游的基因的一个普通集合的表示, ? 包括一些植物生长素应答的基因,在米饭谷物开发期间。在 qTGW3 的 OsSK41 的 loss-of-function 形式代表没被指向的基因编辑或 QTL 节节上升广泛地在 OsSK41 功能的米饭 breeding.?Suppression 利用了的稀罕等位基因提高米饭谷物尺寸和重量。因此,我们的学习在米饭谷物开发揭示 OsSK41 的重要角色 ?? 并且在另外的谷物庄稼在米饭并且也许为谷物产量的基因改进提供新候选人基因。 | Zejun Hu Sun-Jie Lu Mei-Jing Wang Haohua He Le Sun Hongru Wang Xue-Huan Liu Ling Jiang Jing-Liang sun Xiaoyun Xin Wei Kong Chengcai Chu Hong-Wei Xue Jinshui Yang Xiaojin Luo Jian-Xiang Liu | 2018 | Molecular Plant2018，11，5： | 47 |
| 12 | A pilot study on combination of cryosurgery and ^(125)iodine seed implantation for treatment of locally advanced pancreatic cancer显示文摘AIM: To study the therapeutic value of combination o cryosurgery and 125iodine seed implantation for locally advanced pancreatic cancer. METHODS: Forty-nine patients with locally advanced pancreatic cancer (males 36, females 13), with a median age of 59 years, were enrolled in the study. Twelve patients had liver metastases. In all cases the tumors were considered unresectable after a comprehensive evaluation. Patients were treated with cryosurgery, which was performed intraoperatively or percutaneously unde guidance of ultrasound and/or computed tomography (CT), and 125iodine seed implantation, which was performed during cryosurgery or post-cryosurgery under guidance of ultrasound and/or CT. A few patients received regional celiac artery chemotherapy. RESULTS: Thirteen patients received intraoperative cryosurgery and 36 received percutaneous cryosurgery Some patients underwent repeat cryosurgery. 125Iodine seed implantation was performed during freezing procedure in 35 patients and 3-9 d after cryosurgery in 14 cases. Twenty patients, 10 of whom had hepaticmetastases received regional chemotherapy. At 3 mo after therapy, CT was repeated to estimate tumor response to therapy. Most patients showed varying degrees of tumor necrosis. Complete response (CR) of tumor was seen in 20.4% patients, partial response (PR), in 38.8%, stable disease (SD), in 30.6%, and progressive disease (PD), in 10.2%. Adverse effects associated with cryosurgery included upper abdomen pain and increased serum amylase. Acute pancreatitis was seen in 6 patients one of whom developed severe pancreatitis. All adverse effects were controlled by medical management with no poor outcome. There was no therapy-related mortality. During a median follow-up of 18 mo (range of 5-40), the median survival was 16.2 mo, with 26 patients (53.1%) surviving for 12 mo or more. Overall, the 6-, 12-, 24- and 36-mo survival rates were 94.9%, 63.1%, 22.8% and 9.5%, respectively. Eight patients had survival of 24 mo or more. The patient with the longest survival (40 mo) is still living without evidence of tumor recurrence. CONCLUSION: Cryosurgery, which is far less invasive than conventional pancreatic resection, and is associated with a low rate of adverse effects, should be the treatment of choice for patients with locally advanced pancreatic cancer. 125Iodine seed implantation can destroy the residual surviving cancer cells after cryosurgery. Hence, a combination of both modalities has a complementary effect. | Ke-Cheng Xu Li-Zhi Niu Yi-Ze Hu Wei-Bing He Yi-Song He Ying-Fei Li Jian-Sheng Zuo | 2008 | World Journal of Gastroenterology2008，14，10： | 37 |
| 13 | Colorectal cancer lymph node staining by activated carbon nanoparticles suspension in vivo or methylene blue in vitro显示文摘AIM:To investigate whether activated carbon nanoparticles suspension(ACNS) or methylene blue(MB) can increase the detected number of lymph nodes in colorectal cancer.METHODS:Sixty-seven of 72 colorectal cancer patients treated at our hospital fulfilled the inclusion criteria of the study which was conducted from December 2010 to February 2012.Seven patients refused to participate.Eventually,60 patients were included,and randomly assigned to three groups(20 in each group):ACNS group(group A),MB group(group B) and non-stained conventional surgical group(group C).In group A,patients received subserosal injection of 1 mL ACNS in a 4-quadrant region around the mass.In group B,the main artery of specimen was identified and isolated after the specimen was removed,and 2 mL MB was slowly injected into the isolated,stretched and fixed vessel.In group C,no ACNS and MB were injected.All the mesentery lymph nodes were isolated and removed systematically by visually inspecting and palpating the adipose tissue.RESULTS:No difference was observed among the three groups in age,gender,tumor location,tumor diameter,T-stage,degree of differentiation,postoperative complications and peritoneal drainage retention time.The total number of detected lymph nodes was 535,476 and 223 in the three groups,respectively.The mean number of detected lymph nodes per patient was significantly higher in group A than in group C(26.8 ± 8.4 vs 12.2 ± 3.2,P < 0.001).Similarly,there were significantly more lymph nodes detected in group B than in group C(23.8 ± 6.9 vs 12.2 ± 3.2,P < 0.001).However,there was no significant difference between group A and group B.There were 50,46 and 32 metastatic lymph nodes dissected in 13 patients of group A,10 patients of group B and 11 patients of group C,without significant differences among the three groups.Eleven of the 60 patients had insufficient number of detected lymph nodes(< 12).Only one patient with T 4a rectal cancer had 10 lymph nodes detected in group B,the other 10 patients were all from group C.Based on the different diameter categories,the number of detected lymph nodes in groups A and B was significantly higher than in group C.However,there was no statistically significant difference between group A and group B.The metastatic lymph nodes were not significant different among the three groups.Similarly,tumor location,T stage and tumor differentiation did not affect the staining results.Body mass index was a minor influencing factor in the two different staining methods.The stained lymph nodes can easily be identified from the mesenteric adipose tissues,and the staining time for lymph nodes was not significantly different compared with unstained group.None of the patients in groups A and B had drug-related complications.CONCLUSION:Both activated carbon nanoparticles suspension in vivo and methylene blue in vitro can be used as tracers to increase the detected number of lymph nodes in colorectal cancer. | Hong-Ke Cai Hai-Fei He Wei Tian Mei-Qi Zhou Yue Hu Yong-Chuan Deng | 2012 | World Journal of Gastroenterology2012，18，42： | 39 |
| 14 | Prevalence, risk factors, clinical course, and outcome of acute kidney injury in Chinese intensive care units: a prospective cohort study显示文摘 | WEN Ying JIANG Li XU Yuan QIAN Chuan-yun LI Shu-sheng QIN Tie-he CHEN Er-zhen LIN Jian-dong AI Yu- hang WU Da-wei WANG Yu-shan SUN Ren-hua HU Zhen-jie CAO Xiang-yuan ZHOU Fa-chun HE Zhen-yang ZHOU Li-hua AN You-zhong KANG Yan MA Xiao-chun YU Xiang-you ZHAO Ming-yan XI Xiu-ming DU Bin | 2013 | Chinese Medical Journal2013，，23： | 40 |
| 15 | Kappa coefficient: a popular measure of rater agreement显示文摘 | Wan TANG Jun HU HuiZHANG Pan WU Hua HE | 2015 | 上海精神医学2015，27，1： | 37 |
| 16 | One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces显示文摘编辑技术 CRISPR 的指导 RNA 的 DNA 定期聚类 interspaced 短 palindromic repeats/Cas9 被用来介绍双 stranded 闯入染色体并且在细菌指导随后的地点特定的插入 / 删除或基因材料的代替例如 Escherichia coli,链球菌肺病,和乳杆菌 reuteri。在这研究,我们建立了在 Streptomyces 基因操作为使用编辑 plasmid pKCcas9dO 的一个高效率的 CRISPR/Cas9 染色体,它包括目标特定的指南 RNA,优化 codon 的 cas9,和二个指导相同的修理模板。由交付编辑 plasmids 进模型紧张 Streptomyces coelicolor M145 的 pKCcas9dO 系列,通过一步舞 intergeneric 转移,我们完成了与60%100%的高效率在不同层次编辑的染色体,包括单个基因删除,例如 actII-orf4 ,整顿,和 glnR ,并且单个大尺寸的基因簇删除,扮演 21.3 kb , undecylprodigiosin 红 31.6 kb ,和 Ca 2+-dependent 抗菌素例如 actinorhodin 的抗菌素 biosynthetic 簇 82.8 kb 。而且,我们也分别地与 54% 和 45% 的高效率认识到 actII-orf4 和整顿,并且 ACT 和红 biosynthetic 基因簇的同时的删除。最后,我们使用了这个系统介绍核苷酸点变化进 rpsL 基因,它与抵抗授与异种到链球菌。用这个系统,显著地,为一个轮染色体修正要求的时间被三分之一个减少或为常规方法的那些的一个一半。这些结果清楚地显示实质地编辑系统的确定的 CRISPR/Cas9 染色体改进与当前存在的方法相比编辑效率在的染色体 Streptomyces,和它在另外的放线菌种类为申请有诺言到染色体修正。 | He Huang Guosong Zheng Weihong Jiang Haifeng Hu Yinhua Lu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，4： | 37 |
| 17 | Percutaneous cryoablation in combination with ethanol injection for unresectable hepatocellular carcinoma显示文摘AIM: To evaluate the effectiveness and safety of percutaneous hepatic cryoablation in combination with percutaneous ethanol injection (PEI) in patients with unresectable hepaocellular carcinoma (HCC).METHODS: A total of 105 masses in 65 HCC patients underwent percutaneous hepatic cryoablation. The cryoablation was performed with the Cryocare system (Endocare, Irvine, CA, USA) using argon gas as a cryogen.Two freeze-thaw cycles were performed, each reaching a temperature of -180 ℃ at the tip of the probe. PEI was given in 36 patients with tumor masses larger than 6 cm in diameter 1-2 weeks after cryoablation and then once per week for 4 to 6 sessions. The efficacy was evaluated with survival, change of tumor size and alpha-fetoprotein (AFP) levels. RESULTS: During a follow-up duration of 14 months in average with a range of 5 to 21 months, 33 patients (50.8 %)were free of tumors, 22 patients (33.8 %) alive with tumor recurrence: two had bone metastases, three were found to have lung metastases, and the remaining 17 recurrences occurred in the liver, of whom only 3 developed a cryosite recurrence. Among the 41 patients who were followed up for more than one year, 32(78 %) were alive despite of tumor recurrence. Seven patients (10.8 %) died due to disease recurrence. Three patients (4.6 %) died due to some noncancer-related causes. Among the 43 patients who had a CT scan available for review, 38 (88.4 %) had a shrinkage of tumor mass. Among the 22 patients who received biopsies of cryoablated tumor mass, all biopsies except one, showed only dead or scar tissues. Of the patients who had an increased AFP preablatively, 91.3 % had a decrease of AFP to normal or nearly normal levels during postablative 3-6 months. Complications of cryoablation included liver capsular cracking in one patient,transient thrombocytopenia in 4 patients and asymptomatic right-sided pleural effusions in 2 patients. Two patients developed liver abscess at the previous cryoablation site at 2 and 4 months, respectively,following cryoablation, and was recovered after treated with antibiotics and drainage.CONCLUSION: Percutaneous cryoablation offers a safe and possibly curative treatment option for patients with HCC that cannot be surgically removed, and its integration with PEI, may serve as an alternative to partial liver resection in selective patients. | Ke-Cheng Xu Li-Zhi Niu Wei-bin He Zi-Qian Guo Yi-Ze Hu Jian-Sheng Zuo Fuda Cancer Hospital of Guangzhou,Guangzhou 510300,Guangdong Province,China | 2003 | World Journal of Gastroenterology2003，9，12： | 37 |
| 18 | Survival and prognostic factors in hepatitis B virus-related acute-on-chronic liver failure显示文摘AIM:To investigate the survival rates and prognostic factors in patients with hepatitis B virus-related acuteon-chronic liver failure(HBV-ACLF).METHODS:Clinical data in hospitalized patients with HBV-ACLF admitted from 2006 to 2009 were retrospectively analyzed.Their general conditions and survival were analyzed by survival analysis and Cox regression analysis.RESULTS:A total of 190 patients were included in this study.The overall 1-year survival rate was 57.6%.Patients not treated with antiviral drugs had a significantly higher mortality[relative risk(RR)=0.609,P=0.014].The highest risk of death in patients with ACLF was associated with hepatorenal syndrome(HRS)(RR=2.084,P=0.026),while other significant factors were electrolyte disturbances(RR=2.062,P=0.010),and hepatic encephalopathy(HE)(RR=1.879,P<0.001).CONCLUSION:Antiviral therapy has a strong effect on the prognosis of the patients with HBV-ACLF by improving their 1-year survival rate.HRS,electrolyte disturbances,and HE also affect patient survival. | Kun Huang Jin-Hua Hu Hui-Fen Wang Wei-Ping He Jing Chen XueZhang Duan Ai-Min Zhang Xiao-Yan Liu | 2011 | World Journal of Gastroenterology2011，17，29： | 32 |
| 19 | Positioning accuracy assessment for the 4GEO/5IGSO/2MEO constellation of COMPASS显示文摘Determined to become a new member of the well-established GNSS family,COMPASS(or BeiDou-2) is developing its capabilities to provide high accuracy positioning services.Two positioning modes are investigated in this study to assess the positioning accuracy of COMPASS' 4GEO/5IGSO/2MEO constellation.Precise Point Positioning(PPP) for geodetic users and real-time positioning for common navigation users are utilized.To evaluate PPP accuracy,coordinate time series repeatability and discrepancies with GPS' precise positioning are computed.Experiments show that COMPASS PPP repeatability for the east,north and up components of a receiver within China's Mainland is better than 2 cm,2 cm and 5 cm,respectively.Apparent systematic offsets of several centimeters exist between COMPASS precise positioning and GPS precise positioning,indicating errors remaining in the treatments of COMPASS measurement and dynamic models and reference frame differences existing between two systems.For common positioning users,COMPASS provides both open and authorized services with rapid differential corrections and integrity information available to authorized users.Our assessment shows that in open service positioning accuracy of dual-frequency and single-frequency users is about 5 m and 6 m(RMS),respectively,which may be improved to about 3 m and 4 m(RMS) with the addition of differential corrections.Less accurate Signal In Space User Ranging Error(SIS URE) and Geometric Dilution of Precision(GDOP) contribute to the relatively inferior accuracy of COMPASS as compared to GPS.Since the deployment of the remaining 1 GEO and 2 MEO is not able to significantly improve GDOP,the performance gap could only be overcome either by the use of differential corrections or improvement of the SIS URE,or both. | ZHOU ShanShi CAO YueLing ZHOU JianHua HU XiaoGong TANG ChengPan LIU Li GUO Rui HE Feng CHEN JunPing WU Bin | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，12： | 31 |
| 20 | Effects of Traditional Chinese Medicine Shensong Yangxin Capsules on Heart Rhythm and Function in Congestive Heart Failure Patients with Frequent Ventricular Premature Complexes:A Randomized, Double-blind, Multicenter Clinical Trial显示文摘 | Xi Wang Dan HU Song Dang He Huang Cong-Xin Huang Ming-Jie Yuan Yan-Hong Tang Qing-Shan Zheng Fang Yin Shu Zhang Bo-Li Zhang Run-Lin Gao | 2017 | Chinese Medical Journal2017，，14： | 33 |
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