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| 1 | Analysis of ESTs and gene expression patterns of the posterior silkgland in the fifth instar larvae of silkworm, Bombyx mori L.显示文摘The fibroin gene expression pattern and regulation of the posterior silkgland were studied by means of expressed sequence tags (ESTs) using the first and fifth day larvae of the fifth instar of silkworm, Bombyx mori L (strain: C 108). The results showed that there were 911 repetitive ESTs and 1950 single sequences (Singlets) among total 2861 consentient sequences, which were spliced. 1335 sequences were identified and the other 1526 were unknown. 5560 sequences (55.89%) in the posterior silkgland cell of the silkworm were new ESTs without homology with EST data published by Mita et al. The number of repetitive ESTs and single sequences from the first day larvae of the fifth instar was double more than that of the fifth day of the same instar in the silkworms. The unigenes which were more than 50 in repetitive EST size (contig size) came to only about 0.5% in total consentient sequences. There were significant differences between gene expression frequencies, and expressed genes were related to fibroin synthesis and its secretion and fibroin composition. Comparing the fifth day with the first day of the fifth instar, the genes-expressed quantity of fibroin heavy-chain gene was 18 fold higher, fibroin light-chain gene 9 fold and fibroin P52 gene 8 fold. 508 genes functioned for cellular component and 315 for enzyme after function tracing. These results implied that the gene expression of the first day was mainly for preparation for fibroin synthesis except for the growth of silkgland cells, and the gene expression of the fifth day of the fifth instar was mainly for synthesizing and excreting fibroin. Because the ratio of heavy chain, light chain and p25 of fibroin was not 6:6:1 as theoretically expected, or its special H-chain structure, the H-chain gene was not easy to detect through EST technique. Most of genes among total 2861 consentient sequences functioned for fibroin synthesis and secretion. This suggested the fibroin synthesis and secretion procedure of the posterior silkgland was more complex than the knowledge we have. | ZHONG Boxiong, YU Yingpo, XU Yusong, YU Hong, LU Xingmeng, MIAO Yungen, YANG Jun, XU Hao, HU Songnian & LOU Chengfu College of Animal Sciences, Zhejiang University, Hangzhou 310029, China Hangzhou Huada Gene Research and Development Center, Southern Base of Genomic Information, Chinese Academy of Sciences, Hangzhou 310007, China | 2005 | Science China(Life Sciences)2005，48，1： | 8 |
| 2 | Oxidation of As^Ⅲ by Several Manganese Oxide Minerals in Absence and Presence of Goethite显示文摘Oxidation of AsⅢ by three types of manganese oxide minerals affected by goethite was investigated by chemical analysis, equilibrium redox, X-ray diffraction (XRD) and transmission electron microscopy (TEM). Three synthesized Mn oxide minerals of different types, birnessite, todorokite, and hausmannite, could actively oxidize AsⅢ to AsⅤ, and greatly varied in their oxidation ability. Layer structured birnessite exhibited the highest capacity of AsⅢ oxidation, followed by the tunnel structured todorokite. Lower oxide hausmannite possessed much low capacity of AsⅢ oxidation, and released more Mn2+ than birnessite and todorokite during the oxidation. The maximum amount of AsⅤ produced during the oxidation of AsⅢ by Mn oxide minerals was in the order: birnessite (480.4 mmol/kg) > todorokite (279.6 mmol/kg) > hausmannite (117.9 mmol/kg). The oxidation capacity of the Mn oxide minerals was found to be relative to the composition, crystallinity, and surface properties. In the presence of goethite oxidation of AsⅢ by Mn oxide minerals increased, with maximum amounts of AsⅤ being 651.0 mmol/kg for birnessite, 332.3 mmol/kg for todorokite and 159.4 mmol/kg for hausmannite. Goethite promoted AsⅢ oxidation on the surface of Mn oxide minerals through adsorption of the AsⅤ produced, incurring the decrease of AsⅤ concentration in solutions. Thus, the combined effects of the oxidation (by Mn oxide minerals)-adsorption (by goethite) lead to rapid oxidation and immobilization of As in soils and sediments and alleviation of the AsⅢ toxicity in the environments. | FENG Xionghan TAN Wenfeng LIU Fan Huada Daniel RUAN HE Jizheng | 2006 | Acta Geologica Sinica(English Edition)2006，80，2： | 3 |
| 3 | Circle recognition through a 2D hough transform and radius histogramming显示文摘 | Ioannou D Huada W Laine A F | 1999 | Image and vision computing1999，17，1： | 1 |
| 4 | Enhancement of the reductive transformation of pentachlorophenol by polycarboxylic acids at the iron oxide–water interface显示文摘 | Fangbai Li Xugang Wang Yongtao Li Chengshuai Liu Fang Zeng Lijia Zhang Mingde Hao Huada Ruan | 2008 | Journal of Colloid And Interface Science2008，，2： | 1 |
| 5 | Near-infrared and mid-infrared spectroscopic study of sepiolites and palygorskites显示文摘 | Ray L. Frost Oliver B. Locos Huada Ruan J.Theo Kloprogge | 2001 | Vibrational Spectroscopy2001，，1： | 1 |
| 6 | Circle recognition through a2D Hough Transform and radius histogramming显示文摘 | Ioannou D Huada W Laine A F | 1999 | Image and Vision Computing(S0262-8856)1999，17，1： | 1 |
| 7 | TCR–pMHC bond conformation controls TCR ligand discrimination显示文摘A major unanswered question is how a TCR discriminates between foreign and self-peptides presented on the APC surface.Here,we used in situ fluorescence resonance energy transfer(FRET)to measure the distances of single TCR–pMHC bonds and the conformations of individual TCR–CD3ζreceptors at the membranes of live primary T cells.We found that a TCR discriminates between closely related peptides by forming single TCR–pMHC bonds with different conformations,and the most potent pMHC forms the shortest bond.The bond conformation is an intrinsic property that is independent of the binding affinity and kinetics,TCR microcluster formation,and CD4 binding.The bond conformation dictates the degree of CD3ζdissociation from the inner leaflet of the plasma membrane via a positive calcium signaling feedback loop to precisely control the accessibility of CD3ζITAMs for phosphorylation.Our data revealed the mechanism by which a TCR deciphers the structural differences among peptides via the TCR–pMHC bond conformation. | Dibyendu KSasmal Wei Feng Sobhan Roy Peter Leung Yanran He Chufan Cai Guoshuai Cao Huada Lian Jian Qin Enfu Hui Hans Schreiber Erin JAdams Jun Huang | 2020 | Cellular & Molecular Immunology2020，17，3： | 0 |
| 8 | Phase segregation mechanisms of small molecule-polymer blends unraveled by varying polymer chain architecture显示文摘As phase separation between the small-molecule semiconductor and the polymer binder is the key enabler of blend-based organic field-effect transistors(OFETs)fabricated by low-cost solution processing,it is crucial to understand the underlying phase separation mechanisms that determine the phase morphology,which significantly impacts device performance.Beyond the parameter space investigated in previous work,here we investigate the formation of blends by varying the branch architecture of the polymer binder and by shortening the solvent dry time using ultrasonic spray casting.The phase morphologies of the resulting blend films have been thoroughly characterized with a variety of techniques in three dimensions over multiple length scales,including AFM,energy-filtered transmission electron microscope,and neutron reflectivity,and have been correlated with electrical transport performance.From the results,we have inferred that the phase morphology is kinetically determined,limited by the inherent slow movement of polymer macromolecules.The kinetic picture,supported by molecular dynamics modeling,not only consistently explains our observations but also resolves inconsistencies in previous works.The achieved mechanistic understanding will guide further optimization of blend-based organic electronics,such as OFETs and organic photovoltaics. | Jihua Chen Sanjib Das Ming Shao Guoliang Li Huada Lian Jian Qin James F.Browning Jong K.Keum David Uhrig Gong Gu Kai Xiao | 2021 | SmartMat2021，2，3： | 0 |
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