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| 1 | Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana显示文摘Abiotic stresses cause serious crop losses. Knowledge on genes functioning in plant responses to adverse growth conditions is essential for developing stress tolerant crops. Here we report that transgenic expression of MYB15, encoding a R2R3 MYB transcription factor in Arabidopsis thaliana, conferred hypersensitivity to exogenous abscisic acid (ABA) and improved tolerance to drought and salt stresses. The promoter of MYB15 was active in not only vegetative and reproductive organs but also the guard cells of stomata. Its transcript level was substantially upregulated by ABA, drought or salt treatments. Compared with wild type (WT) control, MYB15 overexpression lines were hypersensitive to ABA in germination assays, more susceptible to ABA-elicited inhibition of root elongation, and more sensitive to ABA-induced stomatal closure. In line with the above findings, the transcript levels of ABA biosynthesis (ABA1, ABA2), signaling (ABI3), and responsive genes (AtADH1, RD22, RD29B, AtEM6) were generally higher in MYB15 overexpression seedlings than in WT controls after treatment with ABA. MYB15 overexpression lines displayed improved survival and reduced water loss rates than WT control under water deficiency conditions. These overexpression lines also displayed higher tolerance to NaCl stress. Collectively, our data suggest that overexpression of MYB15 improves drought and salt tolerance in Arabidopsis possibly by enhancing the expression levels of the genes involved in ABA biosynthesis and signaling, and those encoding the stress-protective proteins. | Zhenhua Ding Shiming Li Xueli An Xin Liu Huanju Qin Daowen Wang | 2009 | Journal of Genetics and Genomics2009，36，1： | 42 |
| 2 | Amorphous Zr(OH)4 coated LiNi0.915Co0.075Al0.01O2 cathode material with enhanced electrochemical performance for lithium ion batteries显示文摘LiNi_(0.915)Co_(0.075)Al_(0.01)O_2(NCA) with Zr(OH)_4 coating is demonstrated as high performance cathode material for lithium ion batteries(LIBs). The coated materials are synthesized via a simple dry coating method of NCA with Zr(OH)_4 powders, and then characterized with scanning electron microscopy(SEM), transmission electron microscopy(TEM) and X-ray photoelectron spectroscopy(XPS). Experimental results show that amorphous Zr(OH)_4 powders have been successfully coated on the surface of spherical NCA particles, exhibiting improved electrochemical performance. 0.50 wt% Zr(OH)_4 coated NCA delivers a capacity of 197.6 mAh/g at the first cycle and 154.3 mAh/g after 100 cycles with a capacity retention of 78.1% at 1 C rate. In comparison, the pure NCA shows a capacity of 194.6 mAh/g at the first cycle and 142.5 mAh/g after 100 cycles with a capacity retention of 73.2% at 1 C rate. Electrochemical impedance spectroscopy(EIS) results show that the coated material exhibits a lower resistance, indicating that the coating layer can efficiently suppress transition metals dissolution and decrease the side reactions at the surface between the electrode and electrolyte. Therefore, surface coating with amorphous Zr(OH)_4 is a simple and useful method to enhance the electrochemical performance of NCA-based materials for the cathode of LIBs. | Zhen Zhang Pengfei Zhou Huanju Meng Chengcheng Chen Fangyi Cheng Zhanliang Tao Jun Chen | 2017 | Journal of Energy Chemistry2017，26，3： | 5 |
| 3 | Assessment of the individual and combined effects of Rht8 and Ppd-D1a on plant height, time to heading and yield traits in common wheat显示文摘Grain yield in cereal crops is a complex trait controlled by multiple genes and influenced by developmental processes and environment. Here we report the effects of alleles Rht8 and Ppd-D1 a on plant height, time to heading, and grain yield and its component traits. Association analysis and quantitative trait locus mapping using phenotypic data from 15 environments led to the following conclusions. First, both Rht8 and Ppd-D1 a reduce plant height. However, Ppd-D1 a but not Rht8 causes earlier heading.Second, both Rht8 and Ppd-D1 a promote grain yield and affect component traits. Their combined effects are substantially larger than those conferred by either allele alone.Third, promotion of grain yield by Rht8 and Ppd-D1 a is through increasing fertile spikelet number. We speculate that Rht8 and Ppd-D1 a act independently and additively in control of plant height, grain yield and yield component. Combination of the two alleles is desirable for adjusting plant height and enhancing grain yield and abiotic stress tolerance. | Kunpu Zhang Junjun Wang Huanju Qin Zhiying Wei Libo Hang Pengwei Zhang Matthew Reynolds Daowen Wang | 2019 | The Crop Journal2019，7，6： | 5 |
| 4 | Molecular and Functional Characterization of Sphingosine-1-Phosphate Lyase Homolog from Higher Plants显示文摘Sphingosine-1-phosphate lyase (系统程序设计语言) 涉及降级表明分子 sph-ingosine-1-phosphate 的 conservedsphingolipid。从 Arabidopsis thaliana 和米饭的通常认为的系统程序设计语言蛋白质的生物信息、分子、功能的分析(指定了为 AtSPL 和 OsSPL,分别地) 被进行。氨基酸顺序比较表明植物系统程序设计语言包含了 thepyridoxal 依赖的脱羧酶领域和可以涉及底层催化作用的保存残余。当在 Saccharomyces cerevisiae 表示了时, AtSPL 和 OsSPL 改正了酵母 dpl1 删除紧张的过分敏感的显型,它在内长的系统程序设计语言活动是缺乏的,到长链基于的外长的 sphingolipid (LCB ) ,建议那植物系统程序设计语言蛋白质是在降级的 phosphorylated LCB 的功能的 invivo。在 A。thaliana, AtSPL 抄本在根,茎,叶子,花和 siliques 被检测。在 pAtSPL-AtSPL::GUS 转基因的线, AtSPL::GUS 熔化蛋白质在许多植物、繁殖的纸巾被发现。AtSPL 表示水平动态地在叶开发和老朽期间被调整,并且稳定地并且显著地在 A 被增加。thaliana 幼苗在 A 与房间对待系统程序设计语言的导致死亡的真菌的毒素 fumonisin B1.The 潜在的功能。thaliana 被讨论…… | Yan Niu Kunling Chen Jizhou Wang Xin Liu Huanju Qin Aimin Zhang Daowen Wang | 2007 | Journal of Integrative Plant Biology2007，49，3： | 3 |
| 5 | Overexpressing HRS1 Confers Hypersensitivity to Low Phosphate-Elicited Inhibition of Primary Root Growth in Arabidopsis thaliana显示文摘Phosphate (Pi) deficiency causes dramatic root system architecture(RSA) changes in higher plants.Here we report that overexpression of HRS1 leads to enhanced sensitivity to low Pi-elicited inhibition of primary root growth in Arabidopsis thaliana seedlings.Bioinformatic investigations uncovered that HRS1 and its six homologs encode putative G2-like transcription factors in Arabidopsis.Analysis of promoter::GUS reporter lines revealed that HRS1 transcripts were present mainly in the root hair region and root hair cells under Pi-sufficient conditions.Pi deprivation increased HRS1 expression level and expanded its expression domain.Although HRS1 knockout mutant did not differ from wild type(WT) control irrespective of Pi status,its overexpression lines were significantly more susceptible to low Pi-elicited primary root shortening.In both WT and HRS1 overexpression seedlings,low Pi-induced primary root shortening was accompanied by enhanced root hair cell differentiation,but this enhancement occurred to a greater extent in the latter genotype.Collectively,our data suggest that HRS1 may be involved in the modulation of primary root and root hair growth in Pi-deprived Arabidopsis seedlings,and provide useful clues for further research into the function of HRS1 and its homologs and the mechanisms behind RSA changes under Pi-deficient conditions. | Hong Liu Huixia Yang Chongming Wu Juanjuan Feng Xin Liu Huanju Qin Daowen Wang | 2009 | Journal of Integrative Plant Biology2009，51，4： | 3 |
| 6 | Genetic Analysis of Chromosomal Loci Affecting the Content of Insoluble Glutenin in Common Wheat显示文摘In common wheat, insoluble glutenin(IG) is an important fraction of flour glutenin macropolymers, and insoluble glutenin content(IGC)is positively associated with key end-use quality parameters. Here, we present a genetic analysis of the chromosomal loci affecting IGC with the data collected from 90 common wheat varieties cultivated in four environments. Statistical analysis showed that IGC was controlled mainly genetically and influenced by the environment. Among the major genetic components known to affect end-use quality,1BL/1RS translocation had a significantly negative effect on IGC across all four environments. As to the different alleles of Glu-A1,-B1 and-D1 loci, Glu-A1 a, Glu-B1 b and Glu-D1 d exhibited relatively strong positive effects on IGC in all environments. To identify new loci affecting IGC, association mapping with 1355 DAr T markers was conducted. A total of 133 markers were found associated with IGC in two or more environments(P < 0.05), ten of which consistently affected IGC in all four environments. The phenotypic variance explained by the ten markers varied from 4.66% to 8.03%, and their elite alleles performed significantly better than the inferior counterparts in enhancing IGC. Among the ten markers, w Pt-3743 and w Pt-733835 reflected the action of Glu-D1, and w Pt-664972 probably indicated the effect of Glu-A1. The other seven markers, forming three clusters on 2AL, 3BL or 7BL chromosome arms, represented newly identified genetic determinants of IGC. Our work provided novel insights into the genetic control of IGC, which may facilitate wheat enduse quality improvement through molecular breeding in the future. | Huaibing Jin Zhaojun Wang Da Li Peipei Wu Zhengying Dong Chaowu Rong Xin Liu Huanju Qin Huili Li Daowen Wang Kunpu Zhang | 2015 | Journal of Genetics and Genomics2015，42，9： | 3 |
| 7 | Molecular analysis of lipoxygenase ( LOX ) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants显示文摘 | Bo Feng Zhenying Dong Zhibin Xu Xueli An Huanju Qin Ning Wu Daowen Wang Tao Wang | 2010 | Journal of Cereal Science2010，，3： | 1 |
| 8 | Research status and prospect of wind-vehicle-bridge coupling vibration system显示文摘To promote and develop the theoretical basis and application of the wind-vehicle-bridge coupling vibration system,the corresponding research status and prospects are reviewed and discussed from five aspects,i.e.,the analytical framework,the aerodynamic interference,the evaluation criteria,the design loads of long-span bridge and the double-deck railcum-road bridge.The refining process of analysis system is reviewed from the aspects of simulation wind load,vehicle load and bridge structure,and the corresponding coupling relationship.For aerodynamic interference,the development process is summarized from the simulative precision of the elements(wind,vehicle and bridge),the load cases and the object of interference.For evaluation criteria,the corresponding development course is summarized from the certain evaluation method to uncertain one.For long-span bridge design load,the wind and vehicle loads are reviewed and summarized from current multinational codes and theoretical research.For double-deck rail-cum-road bridge,the mechanism of multi-element coupling relationship and corresponding aerodynamic interference are both reviewed.By comprehensive review and summary,the analytical framework is in the process from simplification to refinement.The simulation and consideration of the objects of structural interference gradually become complex.The corresponding simulation theory,wind tunnel scale,test equipment and technology are the key factors to limit its development.For systematic evaluation of vehicle and bridge,the structural and systemic security are the basis of the evaluation,and the auxiliary components and functional evaluation need to be paid more attention.The evaluation criterion will be developed from certain method to reliability assessment.For design load of long-span bridge,the vehicle load is gradually transferred from the simple application of the design load of small-medium span bridge into a complex model considering the load characteristics.For double-deck rail-cum-road bridge,the basic theory and experimental study on coupling mechanism and aerodynamic interference need to be developed. | Wanshui Han Xiaodong Liu Xuelian Guo Shizhi Chen Gan Yang Huanju Liu | 2022 | Journal of Traffic and Transportation Engineering(English Edition)2022，9，3： | 1 |
| 9 | Preparation and morphological characterization of narrow pore size distributed polypropylene hydrophobic membranes for vacuum membrane distillation via thermally induced phase scparation显示文摘 | Na Tang Qian Jia Huanju hang ct al | 2010 | Dcsalination2010，256，： | 1 |
| 10 | Molecular and functional analysis of phosphomannomutase(PMM)from high-er plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana ben-thamiana显示文摘 | Qian Weiqiang Yu Chunmei Qin Huanju | | 0，，03： | 1 |
| 11 | Unraveling the complexity of polycystic ovary syndrome with animal models显示文摘Polycystic ovary syndrome (PCOS) is a highly familial and heritable endocrine disorder. Over half of the daughters born to women with PCOS may eventually develop their own PCOS-related symptoms. Progress in the treatment of PCOS is currently hindered by the complexity of its clinical manifestations and incomplete knowledge of its etiopathogenesis. Various animal models, including experimentally induced, naturally occurring, and spontaneously arising ones, have been established to emulate a wide range of phenotypical and pathological traits of human PCOS. These studies have led to a paradigm shift in understanding the genetic, developmental, and evolutionary origins of this disorder. Furthermore, emerging evidence suggests that animal models are useful in evaluating state-of-the-art drugs and treatments for PCOS. This review aims to provide a comprehensive summary of recent studies of PCOS in animal models, highlighting the power of these disease models in understanding the biology of PCOS and aiding high-throughput approaches. | Huanju Liu Mixue Tu Zhiyong Yin Dan Zhang Jun Ma Feng He | 2024 | Journal of Genetics and Genomics2024，51，2： | 0 |
| 12 | Carbon dioxide fixation by Chlorella sp.USTB-01 with a fermentor-helical combined photobioreactor显示文摘A promising microalgal strain isolated from fresh water,which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting,was identified as Chlorella sp.USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid(rRNA)gene sequences.In the heterotrophic batch culture,more than 20.0 g·L^(-1)of cell dry weight concentration(DWC)of Chlorella sp.USTB-01 was obtained at day 5,and which was used directly to seed the autotrophic culture.A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp.USTB-01 for the fixation of carbon dioxide(CO_(2)).It showed that the autotrophic growth of Chlorella sp.USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L^(-1)was obtained at day 6.The highest CO_(2)fixation of 95%appeared on day 1 in the exponential growth phases of Chlorella sp.USTB-01 and 49.8%protein was found in the harvested microalgal cells. | Xuan JIA Hai YAN Zijing WANG Huanju HE Qianqian XU Haiou WANG Chunhua YIN Liqin LIU | 2011 | Frontiers of Environmental Science & Engineering2011，5，3： | 0 |
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