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您的检索式：作者名="Huankun Li"
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| 1 | Interleukin 1 beta-induced chloride currents are important in osteoarthritis onset:an in vitro study显示文摘Persistent hypotonic and inflammatory conditions in the joint cavity can lead to the loss of cartilage matrix and cell death,which are the important mechanisms of osteoarthritis(OA)onset.Previous studies have confirmed that the existence of a hypotonic environment is a red flag for inflammation,as hypotonic environment induces the opening of the chloride channel of the cell and promotes chloride ion efflux,which prompts the cell volume to increase.Chloride channels play an important role in the regulation of mineralization and chondrocyte death.Here,we reported that OA chondrocytes showed a significant increase of cell death rate and the imbalance of cartilage matrix catabolism.We found that the distribution of skeleton protein F-actin was disordered.In addition,the volume-sensitive chloride current of OA chondrocytes decreased significantly with the increase of the expression levels of inflammation-related proteins caspase-1,caspase-3,and NLRP3.Moreover,interleukin-1β(IL-1β)showed a potential to activate the chloride current of normal chondrocytes.These results indicate that IL-1β-induced chloride channel opening in chondrocytes may be closely related to the occurrence of OA.This chloride channel opening process may therefore be a potential target for the treatment of OA. | Zhiqin Deng Zicong Lin Qing Zhong Minqiang Lu Huankun Fang Jianquan Liu Li Duan Lixin Chen Liwei Wang Daping Wang Wencui Li | 2021 | Acta Biochimica et Biophysica Sinica2021，53，4： | 2 |
| 2 | CaREM1.4 interacts with CaRIN4 to regulate Ralstonia solanacearum tolerance by triggering cell death in pepper显示文摘Remorins,plant-specific proteins,have a significant role in conferring on plants the ability to adapt to adverse environments.However,the precise function of remorins in resistance to biological stress remains largely unknown.Eighteen CaREM genes were identified in pepper genome sequences based on the C-terminal conserved domain that is specific to remorin proteins in this research.Phylogenetic relations,chromosomal localization,motif,gene structures,and promoter regions of these remorins were analyzed and a remorin gene,CaREM1.4,was cloned for further study.The transcription of CaREM1.4 in pepper was induced by infection with Ralstonia solanacearum.Knocking down CaREM1.4 in pepper using virus-induced gene silencing(VIGS)technologies reduced the resistance of pepper plants to R.solanacearum and downregulated the expression of immunity-associated genes.Conversely,transient overexpression of CaREM1.4 in pepper and Nicotiana benthamiana plants triggered hypersensitive response-mediated cell death and upregulated expression of defense-related genes.In addition,CaRIN4-12,which interacted with CaREM1.4 at the plasma membrane and cell nucleus,was knocked down with VIGS,decreasing the susceptibility of Capsicum annuum to R.solanacearum.Furthermore,CaREM1.4 reduced ROS production by interacting with CaRIN4-12 upon co-injection in pepper.Taken together,our findings suggest that CaREM1.4 may function as a positive regulator of the hypersensitive response,and it interacts with CaRIN4-12,which negatively regulates plant immune responses of pepper to R.solanacearum.Our study provides new evidence for comprehending the molecular regulatory network of plant cell death. | Yanqin Zhang Shuangyuan Guo Feng Zhang Pengfei Gan Min Li Cong Wang Huankun Li Gang Gao Xiaojie Wang Zhensheng Kang Xinmei Zhang | 2023 | Horticulture Research2023，10，5： | 0 |
| 3 | A small knottin-like peptide negatively regulates in wheat to stripe rust resistance during early infection of wheat显示文摘Although Blufensins(Bln)have important functions in the response of plants to biotic stress the precise functioning of Bln in wheat remains largely unknown.Here we isolated a Bln gene(TaBln4)from Suwon 11 infected by Puccinia striiformis f.sp.tritici(Pst).Expression of TaBln4 increased in host plants at the early stage of infection with a virulent Pst race(CYR31)but was unchanged in response to infection by an avirulent race(CYR23).Transcription levels of TaBln4 were also regulated by hormone and abiotic stresses.Expression of TaBln4 in tobacco leaves suppressed Bax-induced programmed cell death.Knockdown of TaBln4 by virus-induced gene silencing inhibited colonization of race CYR31 by increasing the accumulation of H2O2 and formation of hypersensitive responses(HR).Transient overexpression of TaBln4 by a transient overexpression system(BSMV-VOX)increased the susceptibility of wheat to CYR31.Results from bimolecular fluorescence complementation and pull-down assays demonstrated that TaBLN4 interacted with calmodulin.Taken together,our results suggest that TaBln4 negatively regulates resistance in wheat to Pst in a reactive oxygen species(ROS)-and HR-dependent manner. | Shuangyuan Guo Min Li Huankun Li Feng Zhang Qiong Zhang Xueling Huang Xing Li Xiaojie Wang Zhensheng Kang Xinmei Zhang | 2023 | The Crop Journal2023，11，2： | 0 |
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