|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Identification and characterization of two human immunodeficiency virus type 1 unique recombinant forms from Yunnan, China显示文摘Dear Editor,Recombination contributes greatly to the diversity of human immunodeficiency virus type 1(HIV-1).A large number of recombinant strains have been found in China,particularly in Yunnan,which is considered the HIV-1 epicenter of China.Surveillance of unique recombinant forms is helpful for prediction of new circulating recombinant forms.In this study,we identified two | Jianjian Li Lin Li Shaomin Yang Jingyun Li Mi Zhang Cuixian Yang Jiafa Liu Huiqin Li | 2016 | Virologica Sinica2016，31，2： | 4 |
| 2 | An Examination of the Predictability of Tropical Cyclone Genesis in High-Resolution Coupled Models with Dynamically Downscaled Coupled Data Assimilation Initialization显示文摘Predicting tropical cyclone(TC)genesis is of great societal importance but scientifically challenging.It requires fineresolution coupled models that properly represent air−sea interactions in the atmospheric responses to local warm sea surface temperatures and feedbacks,with aid from coherent coupled initialization.This study uses three sets of highresolution regional coupled models(RCMs)covering the Asia−Pacific(AP)region initialized with local observations and dynamically downscaled coupled data assimilation to evaluate the predictability of TC genesis in the West Pacific.The APRCMs consist of three sets of high-resolution configurations of the Weather Research and Forecasting−Regional Ocean Model System(WRF-ROMS):27-km WRF with 9-km ROMS,and 9-km WRF with 3-km ROMS.In this study,a 9-km WRF with 9-km ROMS coupled model system is also used in a case test for the predictability of TC genesis.Since the local sea surface temperatures and wind shear conditions that favor TC formation are better resolved,the enhanced-resolution coupled model tends to improve the predictability of TC genesis,which could be further improved by improving planetary boundary layer physics,thus resolving better air−sea and air−land interactions. | Mingkui LI Shaoqing ZHANG Lixin WU Xiaopei LIN Ping CHANG Gohkan DANABASOGLU Zhiqiang WEI Xiaolin YU Huiqin HU Xiaohui MA Weiwei MA Haoran ZHAO Dongning JIA Xin LIU Kai MAO Youwei MA Yingjing JIANG Xue WANG Guangliang LIU Yuhu CHEN | 2020 | Advances in Atmospheric Sciences2020，37，9： | 4 |
| 3 | Preliminary Investigation of the Clinical Significance of Detecting Circulating Tumor Cells Enriched from Lung Cancer Patients显示文摘 | Chi Wu Huaijie Hao Longyun Li Xiaoyun Zhou Zijian Guo Li Zhang Xiaotong Zhang Wei Zhong Huiqin Guo Ross Macrae Bremner Ping Lin | 2009 | Journal of Thoracic Oncology2009，，1： | 1 |
| 4 | Preliminary Investigation of the Clinical Significance of Detecting Circulating Tumor Cells Enriched from Lung Cancer Patients显示文摘 | Chi Wu Huaijie Hao Longyun Li Xiaoyun Zhou Zijian Guo Li Zhang Xiaotong Zhang Wei Zhong Huiqin Guo Ross Macrae Bremner Ping Lin | 2009 | Journal of Thoracic Oncology2009，，1： | 1 |
| 5 | Effects of rhynchophylline and isorhynchophylline on nitric oxide and endothelin-1 secretion from RIMECs induced by Listeriolysin O in vitro显示文摘 | Xi Chen Jianqin Xu Xiang Mu Yiyi Hu Ge Hu Huiqin Duan Tao Zhang Hong Lin Wei Zhang | 2009 | Veterinary Microbiology2009，，2： | 1 |
| 6 | MgFe-layered double hydroxide modified electrodes for direct electron transfer of heme proteins显示文摘 | Lin Maoguo Ji Huiqin Wang Yinling | 2012 | Biosens Bioelectron2012，38，1： | 1 |
| 7 | Construction of Tobacco Bax Inhibitor-1 ihpRNA Gene Silencing Vector and Transformation into Agrobacterium tumefaciens EHA105显示文摘The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance site and was named as pGSA2285. Using PCR-based amplification, two different restriction sites at both ends of tobacco Bax inhibitor-1 (NtBI-1) gene were created, respectively, which made the construction of ihpRNA gene silencing vector more efficiently. Then, NtBI-1 genes were inserted into Multiple Cloning Site (MCS) of pGSA2285 respectively to form Bax inhibitor-1 ihpRNA gene silencing vector, named as pGSA4285, containing sense and anti-sense BI-1 sequence which was spliced by chalcone synthase intron. Combined PCR identification and enzyme restriction analyses, the results showed that Bax inhibitor-1 ihpRNA gene silencing vector had been constructed and transferred into Agrobacterium tumefaciens EHA105 successfully, which laid a foundation for the further study on the function of BI-1 in plant PCD regulation. | ZHU Wenhua XIE Meng LIN Yuheng YANG Mingyu MA Qiumin TIAN Huiqin QU Guiqin | 2011 | Journal of Northeast Agricultural University(English Edition)2011，18，2： | 0 |
| 8 | Research Progress on Chemical Constituents,Extraction Methods and Pharmacological Effects of Sauropus spatulifolius Beille显示文摘Sauropus spatulifolius Beille is a small evergreen shrub in Sauropus of the Euphorbiaceae family.It mainly contains chemical components such as amino acids,proteins,carbohydrates,polypeptides,glycosides,saponins,tannins,organic acids,alkaloids,coumarin and lactones,volatile oils and oils.In addition,the chemical components obtained by different extraction processes are also different.S.spatulifolius Beille mainly has antitussive and expectorant,anti-inflammatory and analgesic,bacteriostatic,antioxidant,and anti-allergic effects.The rich biological activity of S.spatulifolius Beille makes it have clinical applications such as adjuvant treatment of pulmonary edema,recovery of lung function,and treatment of chronic laryngitis or chronic pharyngitis.In this paper,the main chemical components,extraction and identification methods and biological activity of S.spatulifolius Beille were described in depth,in order to further lay a solid foundation for the in-depth research,development and utilization of S.spatulifolius Beille. | Xin XIE Liyuan LI Chunxiao QIN Tong LI Yajie JING Suoyi HUANG Weijia LIN Xiaolin FU Liqiong ZHOU Huiqin LI Cuixia WANG | 2022 | Medicinal Plant2022，13，6： | 0 |
| 9 | Deep Immunophenotyping of Human Whole Blood by Standardized Multi‑parametric Flow Cytometry Analyses显示文摘Immunophenotyping is proving crucial to understanding the role of the immune system in health and disease.High-through-put flow cytometry has been used extensively to reveal changes in immune cell composition and function at the single-cell level.Here,we describe six optimized 11-color flow cytometry panels for deep immunophenotyping of human whole blood.A total of 51 surface antibodies,which are readily available and validated,were selected to identify the key immune cell populations and evaluate their functional state in a single assay.The gating strategies for effective flow cytometry data analysis are included in the protocol.To ensure data reproducibility,we provide detailed procedures in three parts,including(1)instrument characterization and detector gain optimization,(2)antibody titration and sample staining,and(3)data acquisition and quality checks.This standardized approach has been applied to a variety of donors for a better understanding of the complexity of the human immune system. | Jian Gao Yali Luo Helian Li Yiran Zhao Jialin Zhao Xuling Han Jingxuan Han Huiqin Lin Feng Qian | 2023 | Phenomics2023，3，3： | 0 |
| 10 | Construction and identification of recombinant plasmid pUIS3-BLC^+显示文摘Objective:To construct and identify recombinant plasmid pUIS3-BLC^+.Methods:The cDNA of B-lymphocyte chemoattractant(BLC) was amplified from the total RNA of spleen tissues by PCR method,and were inserted into plasmid of Plasmodium berghei with U1S3 knockout by digestion of restrictive endonuclease and T7 ligation.The recombinant plasmids were screened,and then underwent restriction enzymatic digestion and DNA sequencing.Then the confirmed plasmid was further transfected into COS-1 cells by lipofectamine and the BLC expression was tested by RT-PCR and Western blotting.Results:The cDNA of BLC gene was correctively amplified by RT-PCR and the recombinant plasmid pUIS3-BLC^+ was constructed successfully,which was confirmed by restriction enzymatic digestion and DNA sequencing.RT-PCR and Western blotting also showed the BLC gene expression in COS-1 cells.Conclusions:The recombinant plasmid pUIS3-BLC^+ has BLC expression in COS-1 cells,and is useful for further study on BLC transgene and UIS3 gene knockout in Plasmodium berghei. | Yingzi Lin Shixiang Bao Guanghong Tan Fengying Huang Huiqin Huang | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 0 |
| 11 | Driver mental load identification model Adapting to Urban Road Traffic Scenarios显示文摘load model established in a road traffic scene is difficult to adapt to the changes of the surrounding road environment during the actual Objective:At present,most research on driver mental load identification is based on a single driving scene.However,the driver mental driving process.We proposed a driver mental load identification model which adapts to urban road traffie scenarios.scene discrimination sub-model can quickly and accurately determine the road traffic scene.The driver load identification sub-model Methods:The model includes a driving scene discrimination sub-model and driver load identification sub-model,in which the driving sub-model.selects the best feature subset and the best model algorithm in the scene based on the judgement of the driving scene classification Results:The results show that the driving scene discrimination sub-model using five vehicle features as feature subsets has the best performance.The driver load identification sub-model based on the best feature subset reduces the feature noise,and the recognition tends to be consistent,and the support vector machine(5VM)algorithm is better than the K-nearest neighbors(KNN)algorithm.effect is better than the feature set using a single source signal and all data.The best recognition algorithm in different scenarios Conclusion:The proposed driver mental load identificution model can discriminate the driving scene quickly and accurately,and then identify the driver mental load.In this way,our model can be more suitable for actual driving and improve the effect of driver mental load identification. | Jing Huang Wei Wei Xiaoyan Peng Lin Hu Huiqin Chen | 2023 | Transportation Safety and Environment2023，5，4： | 0 |
| 12 | PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma Progression显示文摘The recurrence and metastasis of children with mediastinal neuroblastoma(NB)are also occurred after surgery,chemotherapy,or radiotherapy.Strategies targeting the tumor microenvironment have been reported to improve survival;however,thorough investigations of monocytes and tumor-associated macrophages(Mϕs)with specialized functions in NB are still lacking.Our data first demonstrated polypyrimidine tract binding protein 2(PTBP2)as a possible identifier in patients with mediastinal NB screened by proteomic profiling and that PTBP2 predicted good outcomes.Functional studies revealed that PTBP2 in NB cells induced the chemotactic activity and repolarization of tumor-associated monocytes and Mϕs,which,in turn,inhibited NB growth and dissemination.Mechanistically,PTBP2 prevents interferon regulatory factor 9 alternative splicing and upregulates signal transducers and activators of transcription 1 to stimulate C-C motif chemokine ligand 5(CCL5)and interferon-stimulated gene factor-dependent type I interferon secretion,to induce monocyte/Mϕs chemotaxis,and to sustain monocytes in a proinflammatory phenotype.Our study defined a critical event of PTBP2-induced monocytes/Mϕs in NB progression and revealed that RNA splicing occurred by PTBP2 benefits immune compartmentalization between NB cells and monocytes.This work revealed the pathological and biological role of PTBP2 in NB development and indicates that PTBP2-induced RNA splicing benefits immune compartmentalization and predicted a favorable prognosis in mediastinal NB. | Jue Tang Jing He Huiqin Guo Huiran Lin Meng Li Tianyou Yang Hai-Yun Wang Di Li Jiabin Liu Le Li Huimin Xia Zhenjian Zhuo Lei Miao | 2023 | Research2023，，3： | 0 |
| 13 | The S100 calcium binding protein A11 promotes liver fibrogenesis by targeting TGF-βsignaling显示文摘Liver fibrosis is a key transformation stage and also a reversible pathological process in various types of chronic liver diseases.However,the pathogenesis of liver fibrosis still remains elusive.Here,we report that the calcium binding protein A11(S100A11)is consistently upregulated in the integrated data from GSE liver fibrosis and tree shrew liver proteomics.S100A11 is also experimentally activated in liver fibrosis in mouse,rat,tree shrew,and human with liver fibrosis.While overexpression of S100A11 in vivo and in vitro exacerbates liver fibrosis,the inhibition of S100A11 improves liver fibrosis.Mechanistically,S100A11 activates hepatic stellate cells(HSCs)and the fibrogenesis process via the regulation of the deacetylation of Smad3 in the TGF-βsignaling pathway.S100A11 physically interacts with SIRT6,a deacetylase of Smad2/3,which may competitively inhibit the interaction between SIRT6 and Smad2/3.The subsequent release and activation of Smad2/3 promote the activation of HSCs and fibrogenesis.Additionally,a significant elevation of S100A11 in serum is observed in clinical patients.Our study uncovers S100A11 as a novel profibrogenic factor in liver fibrosis,which may represent both a potential biomarker and a promising therapy target for treating liver fibrosis and fibrosis-related liver diseases. | Tingting Zhu Linqiang Zhang Chengbin Li Xiaoqiong Tan Jing Liu Huiqin Li Qijing Fan Zhiguo Zhang Mingfeng Zhan Lin Fu Jinbo Luo Jiawei Geng Yingjie Wu Xiaoju Zou Bin Liang | 2022 | Journal of Genetics and Genomics2022，49，4： | 0 |
| 14 | Preparation and Characterization of Red Emitting Phosphor Ba_xCa_(3-x)(PO_4)_2∶Ce, Mn显示文摘Red emitting phosphor (BaCa)3 (PO4)2∶Ce, Mn was prepared by solid state reactions. Effect of chemical component, preparation conditions on the performance of the phosphor was studied by using TG-DTG, XRD and phosphor relative brightness meter. It is found that the host (BaxCa 1-x)3(PO4)2 is present as a distorted Ca3(PO4)2 structure. Ce 3+ acts as a sensitiser, and Mn 2+ is activator. By energy transfer from Ce 3+ to Mn 2+, Mn 2+ is activated and emits red light with wavelength around 650 nm. The phosphor powder shows the highest emitting brightness, when the concentrations of Ce 3+and Mn 2+ are both at 0.15 mol. | Hu Jianguo Wan Guojiang Hu Xuefang Ma Lin Yan Shirun Wang Huiqin Xu Yan | 2005 | Journal of Rare Earths2005，23，5： | 0 |
| 15 | Research Progress in the Application of Lycium Barbarum Polysaccharide in Ophthalmic Diseases显示文摘Lycium barbarum polysaccharides(LBP)are widely used in age-related macular degeneration,glaucoma,diabetic retinopathy,and other eye diseases.With further studies on the pharmacological action of Lycium barbarum polysaccharides in recent years,researchers have found that Lycium barbarum polysaccharides(LBP)play a protective role in the treatment of various eye diseases through anti-oxidation,scavenging oxygen free radicals,and inhibiting inflammatory reaction as well as cell senescence.This paper reviews the application of Lycium barbarum polysaccharides in ophthalmology. | Yanna Lin Huiqin Wu | 2022 | Proceedings of Anticancer Research2022，6，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员