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| 1 | Introduction of rice chitinase gene into wheat via low energy Ar^+ beam implantation显示文摘A chitinase gene (RCH8) in plasmid vector pCAMBIA1308 was delivered into 3 wheat cultivars (Yangmai 158, Wan 9210, Wanmai 32) by low energy Ar+ beam-mediated method. Preliminary calli from treated mature embryos were first selected on hygromycin (Hm, 20 or 30 mg/L) containing medium. After the resistant calli formed, they were transferred to the regeneration medium with 10 or 20 mg/L Hm. All the three wheat varieties obtained trans-genie plants. PCR and PCR-Southern assays showed that most plants regenerated from the resistant calli were positive transgenic plants. Southern blot of the positive green plants confirmed stable integration of alien DNA into wheat genome. The plant transformation frequencies varied with the variety and ion dose implanted. Wanmai 32 possessed the highest transformation frequency, reaching 3.8% at a suitable implantation dose. The transformation frequency of Yangmai 158 and Wan 9210 varied from 0.5% to 2.5% and from 0.5% to 1.4%, respectively. Progeny test for resistance to | WU Lifang LI Hong FENG Huiyun WU Lijun YU Zengliang | 2001 | Chinese Science Bulletin2001，46，4： | 14 |
| 2 | Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain显示文摘The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the re- covered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect im- munofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we ob- tained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the anti- genicity, virulence, pathogenesis, maturation and replication of SVDV. | ZHENG Haixue LIU Xiangtao SHANG Youiun WU Jinyan BAI Xingwen JIN Ye SUN Shiqi GUO Huichen TIAN Hong FENG Xia YIN Shuanghui GUO Jianhong CONG Guozheng LIU Zaixin CHANG Huiyun MA Junwu XIE Qingge | 2006 | Chinese Science Bulletin2006，51，17： | 6 |
| 3 | Synthesis of cyclic peptide reniochalistatin E and conformational isomers显示文摘Here, we report a convenient and efficient synthesis strategy for the total synthesis of cyclic peptide reniochalistatin E and its conformational isomers with 32% overall yield. We found the linear peptide precursor without side chain gave better cyclization yield. | Huiyun Luo Hongli Yin Chaojun Tang Ping Wang Feng Liang | 2018 | Chinese Chemical Letters2018，29，7： | 1 |
| 4 | Ion implantation of organisms 显示文摘 | FENG Huiyun YU Zengliang CHU P K | 2006 | Materials Science and Engineering2006，54，34： | 1 |
| 5 | Utilizing low-energy ion beams to study living organisms显示文摘 | Feng Huiyun Liu Xuelan Yuan Hang | 2007 | Surface and Coatings Technology2007，20，1920： | 1 |
| 6 | Ion implantation of organisms 显示文摘 | FENG Huiyun YU Zengliang CHU P K | 2006 | Materials Science and Engineering2006，54，34： | 1 |
| 7 | Ion implantation of organisms显示文摘 | Huiyun Feng Zengliang Yu Paul K. Chu | 2006 | Materials Science & Engineering R2006，，3： | 1 |
| 8 | Ion implantation of organisms 显示文摘 | Huiyun Feng Zengliang Yu Paul K Chu | 2006 | Materials Science and Engineering2006，54，34： | 1 |
| 9 | Synthesis of a Heptapeptide and Its Application in the Detection of Mercury(Ⅱ) Ion显示文摘A new heptapeptide, Cys-Pro-Gly-Cys-Pro-Gly-Cys-CONH2(P7), was prepared via Fmoc solid-phasepepfide synthesis, purified by high performance liquid chromatography(HPLC) and characterized by mass spectro-metry(MS). The interactions of P7 with metal ions were also investigated by spectroscopy and the results show thatP7 has highly selective binding affinity to Hg(Ⅱ). Based on this specific character, a simple, rapid and sensitivedetection method for Hg(Ⅱ) was developed. When the mixture solution of P7 and Hg(Ⅱ) was added to colloidal goldnanoparticles(GNP), the color of GNP remained unchanged. On the contrary, the color of GNP changed from red toblue when the mixture solutions of P7 and other metal ions were added. | FENG Huiyun GAO Lei YE Xinhui WANG Lei XUE Zechun KONG Jinming LI Lianzhi | 2017 | Chemical Research in Chinese Universities2017，33，2： | 1 |
| 10 | Long-Term Patterns of Meningitis Mortality:A Continual Downward Trend and a Vulnerable Infant Population—China,1987–2021显示文摘Summary What is already known about this topic?Meningitis,a life-threatening disease,presents a significant public health challenge.Its rate of progress in burden reduction notably lags behind other diseases that can be prevented through vaccination.What is added by this report?This research explored the changes in the mortality rate of meningitis in China over a span of 35 years.The study further identified the effects of age,period,and cohort on the mortality trends. | Yu Wu Huiyun Fan Binbin Su Chao Guo Luzhao Feng | 2023 | China CDC weekly2023，5，34： | 0 |
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