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| 1 | Expression,deleton and mnutation of ρ16 gene in human gastric cancer显示文摘AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma.METHODS The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma.RESULTS Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P<0.05). The positive rate of P16 protein expression in mucoid carcinoma 10.00% (1/ 10) was significantly lower than that in poorly differentiated carcinoma 51.22% ( 21/ 41 ),undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/ 16) (P<0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P<0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas.CONCLUSIONS The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph metastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis. | Xiu-Sheng He Qi Su Zhu-Chu Chen Xiu-Tao He Zhi-Feng Long Hui Ling Liang-Run Zhang Oncology Institute,Nanhua University,Hengyang 421001,Hunan Province,ChinaOncology Institute,Center South University,Changsha 410078,Hunan Province,China Department of Gastroenterology,First People’s Hospital of Changde City,Changde 415003,Hunan Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 40 |
| 2 | POSITIVE SOLUTIONS TO m-POINT BOUNDARY VALUE PROBLEM OF NONLINEAR FRACTIONAL DIFFERENTIAL EQUATION WITH p-LAPLACIAN显示文摘In this paper,we are concerned with the existence of positive solutions to an m-point boundary value problem with p-Laplacian of nonlinear fractional differential equation.By means of Krasnosel'skii fixed-point theorem on a convex cone and Leggett-Williams fixed-point theorem,the existence results of solutions are obtained. | Anping Chen~1,2,Yi Chen~1 (1.School of Math.and Computational Science,Xiangtan University,Xiangtan 411005,Hunan 2.Dept.of Math.,Xiangnan University,Chenzhou 423000,Hunan) | 2011 | Annals of Differential Equations2011，27，4： | 15 |
| 3 | Biological characteristics of an improved triploid crucian carp显示文摘An improved triploid crucian carp(ITCC) was produced by crossing improved tetraploids(G1×AT,) with improved red crucian carp(IRCC,),which were obtained by distant crossing and gynogenesis.The biological characteristics of ITCC,including the number and karyotype of chromosomes,gonadad and pituitary structures,phenotype,and growth rate are reported.ITCC possessed 150 chromosomes with the karyotype 33m+51sm+33st+33t.In the breeding season,both ovary-like and testis-like gonads of ITCC were unable to produce normal mature gametes.The ultrastructure of the pituitary of ITCC showed that most of the endocrine granules in gonadotrophic hormone(GTH) cells had not been released,providing endocrinological evidence for the sterility of ITCC.Compared with triploid crucian carp(TCC) produced by mating Japanese crucian carp with allotetraploid hybrids,ITCC not only retained the excellent traits of fast growth rate and sterility,but also acquired improved morphological characteristics,including higher body,shorter tail and smaller head. | CHEN Song,WANG Jing,LIU ShaoJun,QIN QinBo,XIAO Jun,DUAN Wei,LUO KaiKun,LIU JinHui & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China,College of Life Sciences,Hunan Normal University,Changsha 410081,China | 2009 | Science China(Life Sciences)2009，52，8： | 14 |
| 4 | The cloning of Dmc1 cDNAs and a comparative study of its expression in different ploidy cyprinid fishes显示文摘Dmc1 (disrupted meiotic cDNA) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. In this investigation, we cloned the partial cDNAs of Dmc1 of diploid red crucian carp, Japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the DMC1 protein in yeast, mouse and human. The full length cDNAs were then obtained by rapid amplification of cDNA ends (RACE). Our data showed that the full length cDNAs of Dmc1 in the three diploid fishes are all 1375 bp long, while it is 1383 bp long in triploids and 1379 bp long in allotetraploids. And despite of the variation in length, all the cDNAs encode a protein of 342 amino acids. A high homology of 97.3% of the DMC1 protein can be drawn by comparing the amino acid sequences in the three diploids, which is also of 86%, 86% and 95% similarity to human, mouse and zebrafish, respectively. A comparative study of the expression pattern of Dmc1 was carried out by RT-PCR using specific primers against the same se-quences of coding regions in different ploidy cyprinid fishes, from which it was showed that Dmc1 was expressed only in gonads of these five kinds of fishes. The expression pattern of Dmc1 in both ovaries and testes from different ploidy fishes within breeding season was also studied by Real-time PCR, and the results showed that the expression of this gene was greatly different among the three different ploidy fishes, which was the highest of triploid and lowest of allotetraploids. The histological sections data showed matured gonads of both diploid red crucian carp and allotetraploids in breeding season, although the latter demonstrated a higher maturation, and no gonadal maturation could be observed in triploids. In conclusion, we suggest that Dmc1 is specifically expressed in the period of meiosis in all the ploidy cyprinid fishes and directly related with the development of gonad in a manner of ploidy-independent way. And further, the high expression of Dmc1 in female triploids might be associ-ated with abnormal meiosis and sterility. | TAO Min, LIU ShaoJun, LONG Yu, ZENG Chen, LIU JiFang, LIU LiangGuo, ZHANG Chun, DUAN Wei & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2008 | Science China(Life Sciences)2008，51，1： | 13 |
| 5 | Cell-SELEX-based aptamer-conjugated nanomaterials for enhanced targeting of cancer cells显示文摘Early detection and treatment of cancer depends on developing highly sensitive and specific methods for targeting cancer cells. To do this, aptamers, which are generated by a novel technique called cell-SELEX (systematic evolution of ligands by exponential enrichment), have been widely applied in cancer cell targeting based on such merits as high target affinity and specificity, small size, minimal immunogenicity, and ease of chemical modification. Furthermore, aptamers can gain more flexibility as cancer cell targeting tools when conjugated to nanomaterials, including metallic nanoparticles, quantum dots, silica nanoparticles, and carbon nanomaterials, among others. In this review, we discuss the use of cell-SELEX-based aptamer-nanomaterials conjugates as novel molecular tools for enhanced targeting of cancer cells. | KONG RongMei, CHEN Zhuo, YE Mao, ZHANG XiaoBing & TAN WeiHong State Key Laboratory for Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China | 2011 | Science China Chemistry2011，54，8： | 13 |
| 6 | Fura-2/AM法测定突触体内游离Ca^(2+)浓度的方法显示文摘采用新型Ca2+荧光指示剂Fura-2,测定冷冻伤性脑水肿时突触体内游离钙的离子浓度。 | Chen Liuhua Yan Yujia He Zhengwen, et al(Neurological and Neurosurgical Institute of Ncurosurgery, Xiangya Hospital, Hunan Medical University, Changsha 410008) | 1998 | 湖南医科大学学报1998，23，4： | 9 |
| 7 | Formation and biological characterization of three new types of improved crucian carp显示文摘The improved tetraploids (G1×AT) were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2% among G1×AT. After mating with each other, the high-body in- dividuals produced three kinds of bisexual fertile diploid fishes: high-body red crucian carp, high-body fork-like-tails goldfish and gray common carp. The high-body red crucian carp mating with each other formed three types of improved crucian carp (ICC) including improved red crucian carp (IRCC), im- proved color crucian carp (ICCC) and improved gray crucian carp (IGCC). The phenotypes, chromo- some numbers, gonadal structure and fertility of the three kinds of ICC and their offspring were observed. All the three kinds of ICC possessed some improved phenotypes such as higher body, smaller head and shorter tail. The ratios of the body height to body length of IRCC, ICCC and IGCC were 0.54, 0.51 and 0.54, respectively. All of them were obviously higher than that of red crucian carp 0.41 (P<0.01). Three kinds of ICC had the same chromosome number as red crucian carp with 100 chromosomes. All the ICC possessed normal gonads producing mature eggs or sperm, which was important for the production of an improved diploid population. Compared with red crucian carp, all the ICC had stronger fertility such as higher gametes production, higher fertilization rate and higher hatchery rate. Three types of improved diploid fish population were generated from the three kinds of ICC by self-crossing, respectively. The ICC can serve as ornamental fish and edible fish. They are also ideal parents to produce triploids by mating with tetraploids. The new ICC plays an important role in biological evolution and fish genetic breeding. | WANG Jing, QIN QinBo, CHEN Song, LIU ShaoJun, DUAN Wei, LIU JinHui, ZHANG Chun, LUO KaiKun, XIAO Jun & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2008 | Science China(Life Sciences)2008，51，6： | 8 |
| 8 | On the Cauchy Problem for Certain System of Semilinear Parabolic Equations | Jian Huaiyu Chen Donggui Department of Applied Mathematics, Tsinghua University, Beijing 100084, China Yueying Teacher’s College, Hunan 414000, China | 1998 | Acta Mathematica Sinica,English Series1998，14，1： | 5 |
| 9 | A priori error analysis of a discontinuous Galerkin approximation for a kind of compressible miscible displacement problems显示文摘A kind of compressible miscible displacement problems which include molecular diffusion and dispersion in porous media are investigated.A symmetric interior penalty discontinuous Galerkin (SIPG) method is applied to the coupled system of flow and transport.Using the induction hypotheses instead of the cut-off operator and the interpolation projection properties,a priori hp error estimates are presented.The error bounds in L2(H1) norm for concentration and in L∞(L2) norm for velocity are optimal in h and suboptimal in p with a loss of power 1/2. | YANG JiMing1,& CHEN YanPing2 1College of Science,Hunan Institute of Engineering,Xiangtan 411104,China 2School of Mathematical Sciences,South China Normal University,Guangzhou 510631,China | 2010 | Science China Mathematics2010，53，10： | 3 |
| 10 | On the average crosscap number II: Bounds for a graph显示文摘The bounds are obtained for the average crosscap number. Let G be a graph which is not a tree. It is shown that the average crosscap number of G is not less thanβ(G)-1/2β(G)-1β(G) and not larger thanβ(G). Furthermore, we also describe the structure of the graphs which attain the bounds of the average crosscap number. | Yi-chao CHEN & Yan-pei LIU College of Mathematics and Econometrics, Hunan University, Changsha 410082, China Department of Mathematics, Beijing Jiaotong University, Beijing 100044, China | 2007 | Science China Mathematics2007，50，2： | 3 |
| 11 | Analysis theory of random energy of train derailment in wind显示文摘Based on the analysis theory of random energy of train derailment, an analysis theory of random energy of train derailment in wind is suggested. Two methods are proposed -the time domain method and the frequency domain method of analysis theory of random energy of train derailment in wind. The curves of σ pw -v under various wind speeds are obtained through the computation. The original curve of σ p -v is expanded, which turns the analysis theory of random energy of train derailment into the all-weather theory. Train derailment condition has been established under wind action. The first and second criterions of train derailment have been proposed in light of wind action. The analysis of train derailment cases at home or abroad is made, in- cluding the first analysis of Xinjiang train derailment case encountered 13-level of gale, which explained the inevitability of train derailment. The analysis theory of random energy of train derailment in wind shows its validity and accuracy. The input energy σ pw of the transverse vibration of train-track(bridge)-wind system is linked to train speed. With the establishment of the analysis theory of random energy of train derailment in wind, It is likely to initiate an all-weather speed limit map for a train or any high-speed train. | CHEN RuiLin 1,2,3 , ZENG QingYuan 3 , HUANG YunQing 1 , XIANG Jun 3 , WEN Ying 3 , GUO XiaoGang 2 , YIN ChangJun 2 , DONG Hui 2 & ZHAO Gang 4 1 Hunan Key Laboratory for Computation and Simulation in Science and Engineering, Xiangtan University, Xiangtan 411105, China 2 School of Civil Engineering & Mechanics, Xiangtan University, Xiangtan 411105, China 3 School of Civil Architecture, Central South University, Changsha 410075, China 4 AMEC, Toronto M5A5G7, Canada | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，4： | 2 |
| 12 | Comparability and s-Comparability of Modules over Exchange Rings | Huan Yin CHEN Department of Mathematics,Hunan Normal University,Changsha 410006 P.R.China E-mail:chyzxl@sparc2.hunnu.edu.cnFu An LI Institute of Mathematics,Academy of Mathematics and System Sciences Chinese Academy of Sciences,Beijing 100080,P.R.China E-mail:fal@math08.math.ac.cn | 2001 | Acta Mathematica Sinica,English Series2001，17，3： | 2 |
| 13 | Effect of matrix cracking on the time delayed buckling of viscoelastic laminated circular cylindrical shells显示文摘The effect of matrix cracking on the bifurcation creep buckling of viscoelastic laminated circular cylindrical shells is investigated.The viscoelastic behavior of laminas is modeled by Schapery's integral constitutive equation with growing matrix cracks.The values of damage variables are correlated to non-dimensional density of matrix cracks relying on the formulas from mesomechanics approach,and the evolution equation predicting the growth rate of density of matrix cracks is assumed to follow a power type relation with transverse tensile stress.The governing equations for prebuckling creep deformation and bifurcation buckling of laminated circular cylindrical shells under axial compression are obtained on the basis of the Donnell type shallow shell theory and Kármán-Donnell geometrically nonlinear relationship.Corresponding solution strategy is constructed by integrating finite-difference technique,trigonometric series expansion method and Taylor's numerical recursive scheme for convolution integration.The bifurcation creep buckling of symmetrically laminated glass-epoxy circular cylindrical shells with matrix creep cracking coupled are examined for various geometrical parameters and parameters of damage evolution as well as boundary conditions.The numerical results show that matrix creep cracking remarkably shortens the critic time of bifurcation buckling and reduces the durable critic loads,and its effects become weak and finally vanish with the increase of the ratio of radius to thickness in the case of short laminated circular cylindrical shells,also the influence of the matrix creep cracking is mainly dependent on the boundary conditions at two ends for moderately long circular cylindrical shells. | PENG Fan,FU YiMing & CHEN YaoJun College of Mechanics and Aerospace,Hunan University,Changsha 410082,China | 2008 | Science China(Technological Sciences)2008，51，12： | 2 |
| 14 | ACID ELECTROLYTIC TREATMENT PROCESS OF SCRAP BATTERY SLUDGE显示文摘ACIDELECTROLYTICTREATMENTPROCESSOFSCRAPBATTERYSLUDGE①ChenWeiping,ZengYue,YangXia,ChenXinlan,JingYanboInstituteofEnvironmental... | Chen Weiping, Zeng Yue, Yang Xia, Chen Xinlan, Jing Yanbo Institute of Environmental Protection, Hunan University, Changsha 410082 | 1997 | 中国有色金属学会会刊:英文版1997，7，4： | 2 |
| 15 | BASIC ELECTROLYTIC METHOD FOR RECOVERY OF LEAD FROM SCRAP BATTERIES显示文摘BASICELECTROLYTICMETHODFORRECOVERYOFLEADFROMSCRAPBATTERIES￥ChenWeiping;TianYizhuang;BianKejun;ZhengYue(InstituteforEnvironmen... | Chen Weiping Tian Yizhuang Bian Kejun Zheng Yue(Institute for Environmental Protection,Hunan University,Changsha 410082) | 1996 | 中国有色金属学会会刊:英文版1996，6，4： | 2 |
| 16 | Residual stress and radial stress gradient in polycrystalline diamond compacts显示文摘The differential thermal expansion of the polycrystalline diamond layer and the tungsten carbide substrate results in large residual stresses as PDC cutters cooling after sintering.The residual stresses on the top surface of the diamond layer of PDC were measured at five points along the radial direction of PDC using X-ray Diffraction Residual Stress Instrument,thus the stresses and their radial distribution were obtained.The results show that the stresses on the diamond surface are compressive,the biggest stress appears at the central point(about 1200 MPa),and that from the center to the edge of PDC,the magnitude of the stress decreases. A finite element analysis(FEA) was made to check the validity of the testing results.The FEA modeling results were found to correlate well with the measured values.Factors leading to the deviation between XRD experimental measurements and the calculations of residual stress by FEA were also analyzed. | Xu Guoping~(1,2),Yin Zhimin~1,Chen Qiwu~2,Xu Gen~1 (1.School of Materials Science & Engineering,Central South University,Changsha 410083,Hunan,China) (2.Changsha research institute of mining and metallurgy,Changsha 410012,Hunan,China) | 2008 | 金刚石与磨料磨具工程2008，28，S1： | 2 |
| 17 | The Comparable Structure of Exchange Rings | CHEN Huan Yin QIN Hou Rong Department of Mathematics. Hunan Normal University. Changsha 410006. P. R. China Department of Mathematics. Nanjing University. Nanjing 210093. P. R. China | 2002 | Acta Mathematica Sinica,English Series2002，18，1： | 1 |
| 18 | Structural parameterization and functional prediction of antigenic polypeptome sequences with biological activity through quantitative sequence-activity models (QSAM) by molecular electronegativity edge-distance vector (VMED)显示文摘Only from the primary structures of peptides, a new set of descriptors called the molecular electro-negativity edge-distance vector (VMED) was proposed and applied to describing and characterizing the molecular structures of oligopeptides and polypeptides, based on the electronegativity of each atom or electronic charge index (ECI) of atomic clusters and the bonding distance between atom-pairs. Here, the molecular structures of antigenic polypeptides were well expressed in order to propose the auto-mated technique for the computerized identification of helper T lymphocyte (Th) epitopes. Furthermore, a modified MED vector was proposed from the primary structures of polypeptides, based on the ECI and the relative bonding distance of the fundamental skeleton groups. The side-chains of each amino acid were here treated as a pseudo-atom. The developed VMED was easy to calculate and able to work. Some quantitative model was established for 28 immunogenic or antigenic polypeptides (AGPP) with 14 (1― 14) Ad and 14 other restricted activities assigned as '1'(+) and '0'(-), respectively. The latter comprised 6 Ab(15-20), 3 Ak(21-23), 2 Ek(24-26), 2 H-2k(27 and 28) restricted sequences. Good results were obtained with 90% correct classification (only 2 wrong ones for 20 training samples) and 100% correct prediction(none wrong for 8 testing samples); while con-trastively 100% correct classification (none wrong for 20 training samples) and 88% correct classification (1 wrong for 8 testing samples). Both stochastic samplings and cross valida-tions were performed to demonstrate good performance. The described method may also be suitable for estimation and prediction of classes I and II for major histocompatibility an-tigen (MHC) epitope of human. It will be useful in immune identification and recognition of pro-teins and genes and in the design and devel-opment of subunit vaccines. Several quantitative structure activity relationship (QSAR) models were developed for various oligopeptides and polypeptides including 58 dipeptides and 31 pentapeptides with angiotensin converting enzyme (ACE) inhibition by multiple linear regression (MLR) method. In order to explain the ability to characterize molecular structure of polypeptides, a molecular modeling investigation on QSAR was performed for functional prediction of polypeptide sequences with anti-genic activity and heptapeptide sequences with tachykinin activity through quantitative se-quence-activity models (QSAMs) by the molecular electronegativity edge-distance vector (VMED). The results showed that VMED exhibited both excellent structural selectivity and good activity prediction. Moreover, the results showed that VMED behaved quite well for both QSAR and QSAM of poly-and oli-gopeptides, which exhibited both good estimation ability and prediction power, equal to or better than those reported in the previous references. Finally, a preliminary conclusion was drwan: both classical and modified MED vectors were very useful structural descriptors. Some suggestions were proposed for further studies on QSAR/QSAM of proteins in various fields. | LI ZhiLiang1,2, WU ShiRong1,2, CHEN ZeCong1,2, YE Nancy1,2, YANG ShengXi1,2, LIAO ChunYang1,2, ZHANG MengJun1,2,3, YANG Li1,2, MEI Hu1,2,4, YANG Yan1,2, ZHAO Na1,2, ZHOU Yuan1,2, ZHOU Ping1,2, XIONG Qing1,2, XU Hong1,2, LIU ShuShen1,2, LING ZiHua1,2, CHEN Gang1,2,4 & LI GenRong1,2 1 College of Chemistry and Chemical Engineering/Key Laboratory for Chemobiomedical Science and Engineering under Chongqing Municipality, College of Life Science and Biological Engineering/Key Laboratory for Biomechanics and Tissue Engineering under Ministry of Education, Chongqing University, Chongqing 400044, China 2 State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST at Hunan University, Changsha 410012, China 3 Department of Medical Analysis/PLA Center of Bioinformatics Immunology, Surgeon Third University, Chongqing 400031, China 4 Technology Centre for Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore 139651, Singapore | 2007 | Science China(Life Sciences)2007，50，5： | 1 |
| 19 | Second-order calibration applied to quantification of two active components of Schisandra chinensis in complex matrix显示文摘The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its 'second-order advantage'. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents. | Xiao-Hua Zhang, Hai-Long Wu, Jian-Yao Wang, Yao Chen, Yong-Jie Yu, Chong-Chong Nie, Chao Kang, De-Zhu Tu, Ru-Qin Yu State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China | 2012 | Journal of Pharmaceutical Analysis2012，2，4： | 1 |
| 20 | Comparative Analysis and Fuzzy Selection of Information System Development Approach | CHEN Xiaohong *\ LIU Mai *\ YASUHIKO Takahara ** *College of Business and Management, Central South University of Technology, Changsha, Hunan, P.R. China ** Department of Management and Systems Engineering, China Institute of Technology, Ch | 1998 | Systems Science and Systems Engineering1998，8，1： | 1 |
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