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| 1 | Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells显示文摘BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been developed recently.Bearing in mind that the interplay of various diffusible factors released by endothelial cells(ECs)and osteoblasts(OBs)have a pivotal role in bone growth and regeneration and that adjacent ECs and OBs also communicate directly through gap junctions,we set the focus on the simultaneous application of these cell types together with platelet-rich plasma(PRP)as a growth factor reservoir within ectopic bone tissue engineering constructs.AIM To vascularize and examine osteogenesis in bone tissue engineering constructs enriched with PRP and adipose-derived stem cells(ASCs)induced into ECs and OBs.METHODS ASCs isolated from adipose tissue,induced in vitro into ECs,OBs or just expanded were used for implant construction as followed:BPEO,endothelial and osteogenic differentiated ASCs with PRP and bone mineral matrix;BPUI,uninduced ASCs with PRP and bone mineral matrix;BC(control),only bone mineral matrix.At 1,2,4 and 8 wk after subcutaneous implantation in mice,implants were extracted and endothelial-related and bone-related gene expression were analyzed,while histological analyses were performed after 2 and 8 wk.RESULTS The percentage of vascularization was significantly higher in BC compared to BPUI and BPEO constructs 2 and 8 wk after implantation.BC had the lowest endothelial-related gene expression,weaker osteocalcin immunoexpression and Spp1 expression compared to BPUI and BPEO.Endothelial-related gene expression and osteocalcin immunoexpression were higher in BPUI compared to BC and BPEO.BPEO had a higher percentage of vascularization compared to BPUI and the highest CD31 immunoexpression among examined constructs.Except Vwf,endothelial-related gene expression in BPEO had a later onset and was upregulated and well-balanced during in vivo incubation that induced late onset of Spp1 expression and pronounced osteocalcin immunoexpression at 2 and 8 wk.Tissue regression was noticed in BPEO constructs after 8 wk.CONCLUSION Ectopically implanted BPEO constructs had a favorable impact on vascularization and osteogenesis,but tissue regression imposed the need for discovering a more optimal EC/OB ratio prior to considerations for clinical applications. | Jelena G Najdanović Vladimir J Cvetković Sanja T Stojanović MarijaĐVukelić-Nikolić Jelena MŽivković Stevo J Najman | 2021 | World Journal of Stem Cells2021，13，1： | 3 |
| 2 | Improving dispersion of SnO 2 nanoparticles in Ag–SnO 2 electrical contact materials using template method显示文摘 | Vladan ?osovi? Aleksandar ?osovi? Nade?da Talijan Dragana ?ivkovi? Dragan Manasijevi? Du?ko Mini? | 2013 | Journal of Alloys and Compounds2013，，： | 2 |
| 3 | The association between proinflammatory cytokine polymorphisms and cerebral palsy in very preterminfants显示文摘 | Kapitanovic Vidak H Catela Ivkovi T Joki M | 2012 | Cytokine2012，58，1： | 1 |
| 4 | Phenolic profile and anti-inflammatory activity of three Veronica species显示文摘 | Ivana Beara Jelena ?ivkovi? Marija Lesjak Jelena Risti? Katarina ?avikin Zoran Maksimovi? Teodora Jankovi? | 2014 | Industrial Crops & Products2014，，： | 1 |
| 5 | Monthly measurement of daily timers显示文摘 | Goetzmann W N Ingersoll J Ivkovi Z | 2000 | Journal of Financial and Quantitative Analysis2000，35，03： | 1 |
| 6 | Overuse Injuries in Female Athletes 显示文摘 | Alan Ivkovi Miljenko Franie Ivan Bojanie | 2007 | Croat Med J2007，48，6： | 1 |
| 7 | Neurologic Manifestations of Transplant Complications显示文摘 | Sa?a A. ?ivkovi? Hoda Abdel-Hamid | 2010 | Neurologic Clinics2010，，1： | 1 |
| 8 | Isocratic RP-HPLC method for rutin determination in solid oral dosage forms显示文摘 | Kunti(c) V Peji(c) N Ivkovi(c) B Vuji(c) Z Ili(c) K Mi(c)i(c) S Vukojevi(c) V | | 0，，02： | 1 |
| 9 | Prediction of the thermodynamic properties for the Ga-Sb-Pb ternary system显示文摘 | MANASIJEVI(C) D (Z)IVKOVI(C) D (Z)IVKOVI(C) (Z) | 2003 | Calphad2003，27，4： | 1 |
| 10 | Association of MMP-8 promoter gene polymorphisms with carotid atherosclerosis: Preliminary study显示文摘 | Tamara Djuri? Aleksandra Stankovi? Igor Kon?ar Djordje Radak Lazar Davidovi? Dragan Alavanti? Maja ?ivkovi? | 2011 | Atherosclerosis2011，，2： | 1 |
| 11 | The role of uhrasonography in evaluation of pathomorphological changes in humeroscapular periar- thritis 显示文摘 | Granec D Ivkovi A Belina S | 2010 | Lijec Vjesn2010，910，： | 1 |
| 12 | Neuroimaging and Neurologic Complications after Organ Transplantation显示文摘 | Sa?a?ivkovi? | 2007 | Journal of Neuroimaging2007，，2： | 1 |
| 13 | Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis显示文摘 | Olgica Djurkovi?-Djakovi? Vitomir Djoki? Marija Vujani? Tijana ?ivkovi? Branko Bobi? Aleksandra Nikoli? Ksenija Slavi? Ivana Klun Vladimir Ivovi? | 2012 | Experimental Parasitology2012，，3： | 1 |
| 14 | Relations between mercury fractions and microbial community components in seawater under the presence and absence of probable phosphorus limitation conditions显示文摘Microbial transformations of toxic monomethylmercury(MMHg) and dissolved gaseous mercury(DGM) at the lower levels of the marine food web are not well understood, especially in oligotrophic and phosphorus-limited seas. To examine the effects of probable phosphorus limitation(~PP-limitation) on relations between mercury(Hg) fractions and microorganisms, we determined the total mercury(THg), total methylated mercury(MeHg), DGM, and microbiological and chemical parameters in the Central Adriatic Sea. Using statistical analysis, we assessed the potential microbial effects on Hg transformations and bioaccumulation. Only in the absence of ~PP-limitation conditions(^(NO–P)P-limitation) is MeHg significantly related to most chemical and microbial parameters, indicating metabolism-dependent Hg transformations.The heterotrophic activity of low nucleic acid bacteria(abundant in oligotrophic regions)seems responsible for most of Hg methylation under ^(NO–P)P-limitation. Under these conditions,DGM is strongly related to microbial fractions and chlorophyll a, indicating biological DGM production, which is probably not metabolically induced, as most of these relations are also observed underPP-limitation. MMHg biomagnification was observed through an increased bioaccumulation factor from microseston to mesozooplankton. Our results indicate that Hgtransformations and uptake might be enhanced under ^(NO–P)P-limitation conditions, emphasizing their impact on the transfer of Hg to higher trophic levels. | Igor ?ivkovi? Vesna Fajon Jo?e Kotnik Yaroslav Shlyapnikov Kristina Obu Vazner Ermira Begu Stefanija ?estanovi? Danijela ?anti? Ana Vrdoljak Slaven Jozi? Mladen ?oli? Jelena Lu?i? Jere Ve?a Grozdan Ku?pili? Marin Ordulj Frano Mati? Branka Grbec Natalia Bojani? ?ivana Nin?evi? Gladan Milena Horvat | 2019 | Journal of Environmental Sciences2019，31，1： | 1 |
| 15 | The co-inertia approach in identification of specific microRNA in early and advanced atherosclerosis plaque显示文摘 | Ivan Jovanovi? Maja ?ivkovi? Jasmina Jovanovi? Tamara Djuri? Aleksandra Stankovi? | 2014 | Medical Hypotheses2014，，1： | 1 |
| 16 | Portfolio concentration and the performance of individual investors 显示文摘 | Ivkovi'c Z Sialm C Weisbenner S | 2008 | Journal of Fi- nancial and Quantitative Analysis2008，43，3： | 1 |
| 17 | TMD chronic pain and masseter silent period in psychiatric patients on antidepressivetherapy显示文摘 | Ivkovie N Mladenovic I Petkoei S | 2008 | J Oral Rehabil2008，35，6： | 1 |
| 18 | Graph theory and molecular orbitals显示文摘 | A. Graovac I. Gutman N. Trinajsti? T. ?ivkovi? | 1972 | Theoretica Chimica Acta1972，，1： | 1 |
| 19 | TNF-αgene/protein in tumorigenesis of sporadic colon adenocarcinoma显示文摘 | Kapitanovi?S ?a?ev T Catela Ivkovi?T | 2014 | Exp Mol Pathol2014，97，2： | 1 |
| 20 | Prediction of the Ther- modynamic Properties for the Ga-Sb-Pb Ternary System 显示文摘 | Manasijevi dD ~ivkovi d D ~ivkovi d ~ | 2003 | Calphad2003，27，4： | 1 |
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