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| 1 | Advances in engineering methylotrophic yeast for biosynthesis of valuable chemicals from methanol显示文摘Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have been wildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harsh conditions, methylotrophic yeasts such as Pichia pastoris have been explored as a cell factory for production of proteins and high-value chemicals. Methanol utilization pathway(MUT) is highly regulated for efficient methanol utilization, and the downstream pathways need extensively constructed and optimized toward target metabolite biosynthesis. Here, we present an overview of methanol metabolism and regulation in methylotrophic yeasts, among which we focus on the regulation of key genes involved in methanol metabolism. Besides, the recent progresses in construction and optimization of downstream biosynthetic pathways for production of high value chemicals, such as polyketides, fatty acids and isoprenoids, are further summarized. Finally, we discuss the current challenges and feasible strategies toward constructing efficient methylotrophic cell factories may promote wide applications in the future. | Xingpeng Duan Jiaoqi Gao Yongjin J.Zhou | 2018 | Chinese Chemical Letters2018，29，5： | 8 |
| 2 | Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast显示文摘Current yeast metabolic engineering in isoprenoids production mainly focuses on rewiring of cytosolic metabolic pathway.However,the precursors,cofactors and the enzymes are distributed in various sub-cellular compartments,which may hamper isoprenoid biosynthesis.On the other side,pathway compartmentalization provides several advantages for improving metabolic flux toward target products.We here summarize the recent advances on harnessing sub-organelle for isoprenoids biosynthesis in yeast,and analyze the knowledge about the localization of enzymes,cofactors and metabolites for guiding the rewiring of the sub-organelle metabolism.This review may provide some insights for constructing efficient yeast cell factories for production of isoprenoids and even other natural products. | Xuan Cao Shan Yang Chunyang Cao Yongjin J.Zhou | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 5 |
| 3 | Extrusion-based additive manufacturing of Mg-Zn alloy scaffolds显示文摘Porous biodegradable Mg and its alloys are considered to have a great potential to serve as ideal bone substitutes.The recent progress in additive manufacturing(AM) has prompted its application to fabricate Mg scaffolds with geometrically ordered porous structures.Extrusionbased AM,followed by debinding and sintering,has been recently demonstrated as a powerful approach to fabricating such Mg scaffolds,which can avoid some crucial problems encountered when applying powder bed fusion AM techniques.However,such pure Mg scaffolds exhibit a too high rate of in vitro biodegradation.In the present research,alloying through a pre-alloyed Mg-Zn powder was ultilized to enhance the corrosion resistance and mechanical properties of AM geometrically ordered Mg-Zn scaffolds simultaneously.The in vitro biodegradation behavior,mechanical properties,and electrochemical response of the fabricated Mg-Zn scaffolds were evaluated.Moreover,the response of preosteoblasts to these scaffolds was systematically evaluated and compared with their response to pure Mg scaffolds.The Mg-Zn scaffolds with a porosity of 50.3% and strut density of 93.1% were composed of the Mg matrix and MgZn2second phase particles.The in vitro biodegradation rate of the Mg-Zn scaffolds decreased by 81% at day 1,as compared to pure Mg scaffolds.Over 28 days of static immersion in modified simulated body fluid,the corrosion rate of the Mg-Zn scaffolds decreased from 2.3± 0.9 mm/y to 0.7±0.1 mm/y.The yield strength and Young’s modulus of the Mg-Zn scaffolds were about 3 times as high as those of pure Mg scaffolds and remained within the range of those of trabecular bone throughout the biodegradation tests.Indirect culture of MC3T3-E1 preosteoblasts in Mg-Zn extracts indicated favorable cytocompatibility.In direct cell culture,some cells could spread and form filopodia on the surface of the Mg-Zn scaffolds.Overall,this study demonstrates the great potential of the extrusion-based AM Mg-Zn scaffolds to be further developed as biodegradable bone-substituting biomaterials. | J.Dong N.Tümer M.A.Leeflang P.Taheri L.E.Fratila-Apachitei J.M.C.Mol A.A.Zadpoor J.Zhou | 2022 | Journal of Magnesium and Alloys2022，10，9： | 2 |
| 4 | A novel approach to determine residual stress field during FSW of AZ91 Mg alloy using combined smoothed particle hydrodynamics/neuro-fuzzy computations and ultrasonic testing显示文摘The faults in welding design and process every so often yield defective parts during friction stir welding(FSW).The development of numerical approaches including the finite element method(FEM)provides a way to draw a process paradigm before any physical implementation.It is not practical to simulate all possible designs to identify the optimal FSW practice due to the inefficiency associated with concurrent modeling of material flow and heat dissipation throughout the FSW.This study intends to develop a computational workflow based on the mesh-free FEM framework named smoothed particle hydrodynamics(SPH)which was integrated with adaptive neuro-fiizzy inference system(ANFIS)to evaluate the residual stress in the FSW process.An integrated SPH and ANFIS methodology was established and the well-trained ANIS was then used to predict how the FSW process depends on its parameters.To verify the SPH calculation,an itemized FSW case was performed on AZ91 Mg alloy and the induced residual stress was measured by ultrasonic testing.The suggested methodology can efficiently predict the residual stress distribution throughout friction stir welding of AZ91 alloy. | A.R.Eivani H.Vafaeenezhad H.R.Jafarian J.Zhou | 2021 | Journal of Magnesium and Alloys2021，9，4： | 2 |
| 5 | Highly Virulent Porcine Reproductive and Respiratory Syndrome Virus Emerged in China显示文摘 | Y.‐J.Zhou X.‐F.Hao Z.‐J.Tian G.‐Z.Tong D.Yoo T.‐Q.An T.Zhou G.‐X.Li H.‐J.Qiu T.‐C.Wei X.‐F.Yuan | 2008 | Transboundary and Emerging Diseases (鈥?)2008，，3： | 1 |
| 6 | 查看详情显示文摘 | J.Zhou Z.H.Liu | | 0，，： | 1 |
| 7 | The structure and properties of acrylic-polyurethane hybrid emulsions显示文摘 | M.Hirose J.Zhou N.Katsutoshi | | 0，，： | 1 |
| 8 | Lactadherin functions as a probe for phosphatidylserine exposure and as an anticoagulant in the study of stored platelets显示文摘 | J.Hou Y.Fu J.Zhou W.Li R.Xie F.Cao G. E.Gilbert J.Shi | 2011 | Vox Sanguinis2011，，2： | 1 |
| 9 | Large‐Area Nanowire Arrays of Molybdenum and Molybdenum Oxides: Synthesis and Field Emission Properties显示文摘 | J.Zhou S.‐Z.Deng J.Chen J.‐C.She Z.‐L.Wang | 2003 | Mater2003，，21： | 1 |
| 10 | Sign-changing, solutions, for, some, fourth-order, nonlinear, elliptic, problems显示文摘 | J.Zhou X.Wu | | 0，，： | 1 |
| 11 | 查看详情显示文摘 | X.Zhou J.Zhou K.Lü Q.Sun | | 0，，02： | 1 |
| 12 | Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol显示文摘The methylotrophic yeast Pichia pastoris(syn.Komagataella phaffii)has been extensively engineered for protein production,and is attracting attention as a chassis cell for methanol biotransformation toward production of small molecules.However,the relatively unclear methanol metabolism hampers the metabolic rewiring to improve the biosynthetic efficiency.We here performed a label-free quantitative proteomic analysis of Pichia pastoris when cultivated in minimal media containing methanol and glucose,respectively.There were 243,158 up-regulated proteins and 244,304 down-regulated proteins in log and stationary phase,respectively,when cultivated in methanol medium compared with that of glucose medium.Peroxisome enrichment further improved the characterization of more differentially expressed proteins(481 proteins in log phase and 524 proteins in stationary phase).We demonstrated the transaldolase isoenzyme(Tal2,Protein ID:C4R244)was highly up-regulated in methanol medium cultivation,which plays an important role in methanol utilization.Our work provides important information for understanding methanol metabolism in methyltrophic yeast and will help to engineer methanol biotransformation in P.pastoris. | Rui Hou Linhui Gao Jianhui Liu Zhen Liang Yongjin J.Zhou Lihua Zhang Yukui Zhang | 2022 | Synthetic and Systems Biotechnology2022，7，3： | 1 |
| 13 | Variability of East Asian winter monsoon in Quaternary climatic extremes in North China显示文摘 | Lu H K.Van Huissteden J.Zhou J.Vanderberghe X.Lu Z.An | | 0，，： | 1 |
| 14 | Formation of 0 84nm hydrated kaolinite as an environmentfriendly precursor of kaolinite intercalation显示文摘 | J.Zhou W.Zheng J.F Xu L.Chen Y.Li N.Ma P.Du | | 0，，： | 1 |
| 15 | Determination of Trace Para Red Residues in Foods through On‐Line Molecularly Imprinted Solid Phase Extraction Coupled with High‐Performance Liquid Chromatography显示文摘 | Z.X.Xu J.Zhou D.Y.Zhao X.G.Qiao J.M.Yang | 2010 | Journal of Food Science2010，，1： | 1 |
| 16 | Behavioral modeling of power amplifiers with dynamic fuzzy neural networks显示文摘 | J.Zhai J.Zhou L.Zhang W.Hong | | 0，，09： | 1 |
| 17 | Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha显示文摘Methylotrophic yeast Ogataea polymorpha is capable to utilize multiple carbon feedstocks especially methanol as sole carbon source and energy,making it an ideal host for bio-manufacturing.However,the lack of gene integration sites limits its systems metabolic engineering,in particular construction of genome-integrated pathway.We here screened the genomic neutral sites for gene integration without affecting cellular fitness,by genomic integration of an enhanced green fluorescent protein(eGFP)gene via CRISPR-Cas9 technique.After profiling the growth and fluorescent intensity in various media,17 genome positions were finally identified as potential neutral sites.Finally,integration of fatty alcohol synthetic pathway genes into neutral sites NS2 and NS3,enabled the production of 4.5 mg/L fatty alcohols,indicating that these neutral sites can be used for streamline metabolic engineering in O.polymorpha.We can anticipate that the neutral sites screening method described here can be easily adopted to other eukaryotes. | Wei Yu Jiaoqi Gao Xiaoxin Zhai Yongjin J.Zhou | 2021 | Synthetic and Systems Biotechnology2021，6，2： | 1 |
| 18 | Expression of chemosensory proteins in the tsetse fly Glossina morsitans morsitans is related to female host‐seeking behaviour显示文摘 | R.Liu X.He S.Lehane M.Lehane C.Hertz‐Fowler M.Berriman L. M.Field J.‐J.Zhou | 2012 | Insect Molecular Biology2012，，1： | 1 |
| 19 | Application of closely spaced inclined channels in gravity separation of fine particles显示文摘 | K.P.Galvin J.Zhou K.Walton | | 0，，04： | 1 |
| 20 | Processing of fine particles using closely spaced inclined channels显示文摘 | K.Walton J.Zhou K.P.Galvin | | 0，，04： | 1 |
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