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| 1 | A novel selective autophagy receptor,CCDC50,delivers K63 polyubiquitination-activated RIG-I/MDA5 for degradation during viral infection显示文摘Autophagy is a con served process that delivers cytosolic substa nces to the lysosome for degradatio n,but its direct role in the regulation of antiviral inn ate immu nity remains poorly un derstood.Here,through high-throughput screeni ng,we discovered that CCDC50 functions as a previously unknown autophagy receptor that negatively regulates the type I interferon(IFN)signaling pathway initiated by RIG-I-like receptors(RLRs),the sensors for RNA viruses.The expression of CCDC50 is enhanced by viral infection,and CCDC50 specifically recognizes K63-polyubiquitinated RLRs,thus delivering the activated RIG-I/MDA5 for autophagic degradation.The associatio n of CCDC50 with phagophore membrane protei n LC3 is con firmed by crystal structure analysis.In contrast to other known autophagic cargo receptors that associate with either the LIR-docking site(LDS)or the UIMdocking site(UDS)of LC3,CCDC50 can bind to both LDS and UDS,representing a new type of cargo receptor.In mouse models with RNA virus infection,CCDC50 deficiency reduces the autophagic degradation of RIG-I/MDA5 and promotes type I IFN responses,resulting in enhaneed viral resistance and improved survival rates.These results reveal a new link between autophagy and antiviral innate immune resp on ses and provide additional in sights into the regulatory mecha nisms of RLR-mediated antiviral signaling. | Panpan Hou Kongxiang Yang Penghui Jia Lan Liu Yuxin Lin Zibo Li Jun Li Shuliang Chen Shuting Guo Ji'An Pan Junyu Wu Hong Peng Weijie Zeng Chunmei Li Yingfang Liu Deyin Guo | 2021 | Cell Research2021，31，1： | 5 |
| 2 | Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis显示文摘The shoot apical meristem(SAM)and root apical meristem(RAM)act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants,respectively.The CLAVATA3(CLV3)-WUSCHEL(WUS)negative-feedback loop acts as a core pathway controlling SAM homeostasis,while CLV3/EMBRYO SURROUNDING REGION(ESR)40(CLE40)and WUSCHEL-RELATED HOMEOBOX5(WOX5),homologs of CLV3 and WUS,direct columella stem cell fate.Moreover,CLV3 INSENSITIVE KINASES(CIKs)have been shown to be essential for maintaining SAM homeostasis,whereas whether they regulate the distal root meristem remains to be elucidated.Here,we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root meristem.We found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell(CSC)differentiation.Biochemical assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4(ACR4)in a ligand-independent manner and can be phosphorylated by ACR4 in vitro.In addition,the phosphorylation of CIKs can be rapidly induced by CLE40,which partially depends on ACR4.Although CIKs act as conserved and redundant regulators in the SAM and RAM,our results demonstrated that they exhibit differentiated functions in these meristems. | Yafen Zhu Chong Hu Yanwei Cui Li Zeng Sunjingnan Li Mingsong Zhu Fanhui Meng Shuting Huang Li Long Jing Yi Jia Li Xiaoping Gou | 2021 | Molecular Plant2021，14，7： | 2 |
| 3 | p53 codon 72 polymorphism and breast cancer risk: A meta-analysis显示文摘 | Jing Hou Yuan Jiang Wenru Tang Shuting Jia | 2013 | Experimental and Therapeutic Medicine2013，，5： | 1 |
| 4 | Improved bioproduction of short-chain fatty acids from waste activated sludge by perennial ryegrass addition 显示文摘 | JIA Shuting DAI Xiaohu ZHANG Dong | 2013 | Water Research2013，47，13： | 1 |
| 5 | Improved bioproduction of short-chain fatty acids from waste activated sludge by perennial ryegrass addition显示文摘 | Shuting Jia Xiaohu Dai Dong Zhang | 2013 | Water Research2013，47，13： | 1 |
| 6 | Improved bioproduction of short-chain fatty acids from waste activated sludge by perennial ryegrass addition 显示文摘 | JIA Shuting DAI Xiaohu ZHANG Dong | 2013 | Water Research2013，47，13： | 1 |
| 7 | Pu- erh tea inhibits tumor cell growth by down-regulating mutant p53 显示文摘 | ZHAO Lanjun JIA Shuting TANG Wenru | 2011 | International Journal of Molecular Sci- ences2011，12，11： | 1 |
| 8 | DOPA-IGF-1 Coated HA/PLGA Microspheres Promoting Proliferation and Osteoclastic Differentiation of Rabbit Bone Mesenchymal Stem Cells显示文摘To explore the ability of dihydroxyphenylalanine-insulin-like growth factor-1 (DOPA-IGF-1) coated hydroxyapatite/poly(lactic-co-glycolic acid)(HA/PLGA) microspheres to promote the proliferation and osteoclastic differentiation of rabbit bone mesenchymal stem cells(rBMSCs), HA/PLGA microspheres with different HA content (10%, 30%, 50%, mass fraction) were prepared by electrospinning method and HA/PLGA microspheres with 50% HA were coated with IGF-1 and DOPA-IGF-1, respectively. They were co-cultured with rBMSCs, respctively. Cell counting kit-8(CCK-8) detection, confocal laser scanning microscopy(CLSM), alkaline phosphatase(ALP) detection and osteogenesis related genes COL IA1, Runx2 and bone morphogenetic protein-2(BMP-2) detection were conducted to detect the proliferation activity, cell morphology, differentiation ability and the expression level of osteogenesis-related genes of cells cultured on all microspheres groups. The results showed that rBMSCs proliferation increased in an HA content dependent manner, and cells proliferated more in the IGF-1 coated and DOPA-IGF-1 coated groups, in particular in DOPA-IGF-1 coated group, and the differences were more remarkable over time (P<0.05). HA/PLGA microspheres promoted the proliferation and osteogenic differentiation of rBMSCs, and DOPA-IGF-1 coating enhanced the proliferation and osteogenic differentiation of rBMSCs. | LIU Guomin LI Yalong YANG Shuting ZHAO Yian LU Tiancheng JIA Wenyuan JI Xuan LUO Yungang | 2019 | Chemical Research in Chinese Universities2019，35，3： | 1 |
| 9 | Improved bioproduction of short-chain fatty acids from waste activated sludge by perennial ryegrass addition显示文摘 | Shuting Jia Xiaohu Dai Dong Zhang Lingling Dai Rongchang Wang Jianfu Zhao | 2013 | Water Research2013，，13： | 1 |
| 10 | Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier显示文摘In this study,we designed a Cd^(2+)whole-cell biosensor with both positive and negative feedback cascade am-plifiers in Pseudomonas putida KT2440(LTCM)based on our previous design with only a negative feedback amplifier(TCM).The results showed that the newly developed biosensor LTCM was greatly improved compared to TCM.Firstly,the linear response range of LTCM was expanded while the maximum linear response range was raised from 0.05 to 0.1μM.Meanwhile,adding a positive feedback amplifier further increased the fluorescence output signal of LTCM 1.11–2.64 times under the same culture conditions.Moreover,the response time of LTCM for detection of practical samples was reduced from 6 to 4 h.At the same time,LTCM still retained very high sensitivity and specificity,while its lowest detection limit was 0.1 nM Cd^(2+)and the specificity was 23.29(compared to 0.1 nM and 17.55 in TCM,respectively).In summary,the positive and negative feedback cascade amplifiers effectively improved the performance of the biosensor LTCM,resulting in a greater linear response range,higher output signal intensity,and shorter response time than TCM while retaining comparable sensitivity and specificity,indicating better potential for practical applications. | Shuting Hu Guangbao Zhang Xiaoqiang Jia | 2023 | Synthetic and Systems Biotechnology2023，8，2： | 0 |
| 11 | Cytarabine delivered by CD44 and bone targeting redox-sensitive liposomes for treatment of acute myelogenous leukemia显示文摘Acute myelogenous leukemia(AML)remains a serious fatal disease for the patients and effective treatment strategies are urgently needed.Based on the characteristics of the AML,we developed the CD44 and bone targeting liposomes delivery system decorated with the redox-cleavable polymer.First,ALN-HA was obtained by amination between alendronate(ALN)and hyaluronic acid(HA),and cholesterol(Chol)was coupled by a disulfide linker(-SS-)with biological reducibility to obtain the goal polymer,ALN-HA-SS-Chol,decorated the liposomes loaded with the Cytarabine(AraC).ALN-HA-SS-AraC-Lip exhibited a spherical morphology with the diameter of 117.5nm and expanded at the environment of 10mM dithiothreitol.Besides,compared with other groups,ALN-HA-SS-AraC-Lip showed benign hydroxyapatite affinity in vitro and bone targeting in C57/BL6 mice,also,ALN-HA-SS-AraC-Lip exhibited encouraging antitumor which significantly reduced the white blood cell amount in bone marrow and blood smear caused by AML model,besides,the dual targeting liposomes also prolong the survival time of mice.In conclusion,the bone and CD44 dual targeting liposomes with redox sensitivity could target to the leukemia stem cells regions and then uptake by the tumor cells,which would be a valuable target for the treatment of the AML. | Hao Wu Yuan Gao Jia Ma Maosong Hu Jing Xia Shuting Bao Yuxi Liu Kai Feng | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
| 12 | Mettl13 protects against cardiac contractile dysfunction by negatively regulating C-Cbl-mediated ubiquitination of SERCA2a in ischemic heart failure显示文摘Ischemic heart failure(HF)remains a leading cause of morbidity and mortality.Maintaining homeostasis of cardiac function and preventing cardiac remodeling deterioration are critical to halting HF progression.Methyltransferase-like protein 13(Mettl13)has been shown to regulate protein translation efficiency by acting as a protein lysine methyltransferase,but its role in cardiac pathology remains unexplored.This study aims to characterize the roles and mechanisms of Mettl13 in cardiac contractile function and HF.We found that Mettl13 was downregulated in the failing hearts of mice post-myocardial infarction(MI)and in a cellular model of oxidative stress.Cardiomyocyte-specific overexpression of Mettl13 mediated by AAV9-Mettl13 attenuated cardiac contractile dysfunction and fibrosis in response to MI,while silencing of Mettl13 impaired cardiac function in normal mice.Moreover,Mettl13 overexpression abrogated the reduction in cell shortening,Ca^(2+)transient amplitude and SERCA2a protein levels in the cardiomyocytes of adult mice with MI.Conversely,knockdown of Mettl13 impaired the contractility of cardiomyocytes,and decreased Ca^(2+)transient amplitude and SERCA2a protein expression in vivo and in vitro.Mechanistically,Mettl13 impaired the stability of c-Cbl by inducing lysine methylation of c-Cbl,which in turn inhibited ubiquitination-dependent degradation of SERCA2a.Furthermore,the inhibitory effects of knocking down Mettl13 on SERCA2a protein expression and Ca^(2+)transients were partially rescued by silencing c-Cbl in H_(2)O_(2)-treated cardiomyocytes.In conclusion,our study uncovers a novel mechanism that involves the Mettl13/c-Cbl/SERCA2a axis in regulating cardiac contractile function and remodeling,and identifies Mettl13 as a novel therapeutic target for ischemic HF. | Shuting Yu ZhiYong Sun Xiuzhu Wang Tiantian Ju Changhao Wang Yingqi Liu Zhezhe Qu KuiWu Liu Zhongting Mei Na Li Meixi Lu Fan Wu Min Huang Xiaochen Pang Yingqiong Jia Ying Li Yaozhi Zhang Shunkang Dou Jianhao Jiang Xin Li Baofeng Yang Weijie Du | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 13 | Association Between rs1344706 of ZNF804A and Schizophrenia: A Meta-analysis显示文摘Schizophrenia is one of the most serious mental diseases found in humans. Previous studies indicated that the single nucleotide polymorphism(SNP) rs1344706 in the gene ZNF804 A encoding zinc finger protein 804 A was associated with schizophrenia in Caucasian population but not in Chinese Han population. However, current results are conflicting in Asian population. In the present study, a meta-analysis was performed to revisit the association between rs1344706 and the risk of schizophrenia in Asian, Caucasian and other populations. Electronic search of Pub Med database identified 25 case–control studies with available genotype frequencies of rs1344706 for the meta-analysis,involving a total of 15,788 cases and 22,654 controls. A pooled odds ratio(OR) with 95% confidence interval(CI) was used to assess the association. The current meta-analysis showed an association between rs1344706 and schizophrenia in Caucasian populations(P = 0.028, OR = 1.138, 95% CI:1.014–1.278; P = 0.004 for heterogeneity) and Asian populations(P = 0.008, OR = 1.092, 95%CI: 1.023–1.165; P = 0.001 for heterogeneity), but not in other populations(P = 0.286,OR = 1.209, 95% CI: 0.853–1.714, P = 0.120 for heterogeneity). Egger's test(P > 0.05) and Begg's test(P > 0.05) are both suggestive of the lack of publication bias for the included studies. Thus, the absence of association in other populations suggests a genetic heterogeneity in the susceptibility of schizophrenia and demonstrates the difficulties in replicating genome-wide association study findings regarding schizophrenia across different ethnic populations. To validate the association between rs1344706 and schizophrenia, further studies with larger participant populations worldwide are needed. | Meiyan Zhu Tongyang Liu Jihong Zhang Shuting Jia Wenru Tang Ying Luo | 2014 | Genomics, Proteomics & Bioinformatics2014，12，6： | 0 |
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