|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes显示文摘To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PGR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation. | YINGCHEN ZHIXuHE AILIANLIU KAIWANG WENWEIMAO JIANKINCHU YONGLU ZHENGFUFANG YINGTANGSHI QINGZHANGYANG DAYUANCHEN MINKANGWANG JINSONGLI SHAOLIANGHUANG XIANGYINKONG YAOZHOUSHI ZHIQIANGWANG JIAHuIXIA ZHIGAOLONG ZHIGANGXUE WENXIANGDING HUIZHENSHENG | 2003 | Cell Research2003，13，4： | 56 |
| 2 | STUDY ON AEROSOL TRANSMISSION ROUTES OF HEMORRHAGIC FEVER WITH RENAL SYNDROME (HFRS)显示文摘The present paper reviews our studies on transmission routes and prophylactic measures of Hemorrhagic Fever with Renal Syndrome (HFRS), including aerosol inhalation, skin injury/mucosa, insect vectors, peroral infection and vertical transmission. The results show that HFRS is transmitted by Hantaan virus via multi-routes. One of them, the aerosol transmission, is perhaps the main route transmitting HFRS from mouse to human beings. The injury and mucosa is the main transmission route from mouse to mouse and also an important agent from mouse to human beings.The peroral infection may occur in very serious pollution of foods. The insect vectors may play an important role in the focus of HFRS and in the transmission of HFRS from mouse to human. The epidemiological significance of vertical transmission is yet uncertain. According to the results, four proposals should be emphasized including killing insects and mice, long lasting and combined prophylactics, intensive studies on prophylactic measures on viral aerosols and the blocking of transmission of HFRS from mouse to human via aerosols. | FengxiangChe LingyingMeng JianchunLu JinsongLi JunbaoLi MinxiaLiu | 2003 | China Particuology2003，1，2： | 3 |
| 3 | E‐Cadherin‐Mediated Cell–Cell Contact Is Critical for Induced Pluripotent Stem Cell Generation显示文摘 | TaotaoChen DetianYuan BinWei JingJiang JiuhongKang KunLing YijunGu JinsongLi LeiXiao GangPei | 2010 | STEM CELLS2010，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员