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| 1 | 虹鳟肿瘤坏死因子(TNFα)基因体外表达与纯化的研究显示文摘将虹鳟两种肿瘤坏死因子TNFα1和TNFα2基因的成熟肽编码区域 ,用带有BamHI和HindIII酶切位点的基因特异性引物进行PCR扩增。扩增片段用限制性内切酶消化并连接到pQE30表达载体上 ,连接产物转化到大肠杆菌JM10 9感受态细菌中。转化子经PCR筛选 ,质粒测序 ,完成了虹鳟两种TNFα基因重组子的构建。重组子经体外培养和诱导后 ,获得了高效表达的TNFα重组蛋白。高效表达的重组TNFα不受诱导剂IPTG的影响 ,并且由于重组子高效表达而形成了包涵体 ;重组蛋白产量约占菌体蛋白总量的 2 5 %— 30 %。应用Ni NTA和固定金属亲和层析 (IM PC)技术 ,在变性条件下获得了高度纯化的重组蛋白 ,纯化重组蛋白的产量约为 0 5— 1mg L。 | 蔡中华 宋林生 ZOU Jun C J SECOMBES 董双林 | 2003 | 水生生物学报2003，27，6： | 7 |
| 2 | A new class of doubletalk detectors based on cross-correlation显示文摘 | Senesty J Dennis R M Jun H C | 2000 | IEEE Trans on Speech and Audio Processing2000，8，2： | 1 |
| 3 | Association between age and clinical characteristics of renal cell carcinoma in adult patients 显示文摘 | Jun C Zhishun X Xianzhou J | 2006 | Int J Urol2006，13，5： | 1 |
| 4 | Selection of microalgae for lipid production under high levels carbon dioxide 显示文摘 | YOO C JUN S Y LEE J Y AHN C Y OH H M | 2010 | Bioresoure Technology2010，101，1： | 1 |
| 5 | 查看详情显示文摘 | Kim S D Baek S C Lee Y J Jun K W Kim M J Yoo I S | | 0，，： | 1 |
| 6 | Hemostatic suturing technique for uterine bleeding during cesarean delivery显示文摘 | Cho J H Jun H S Lee C N | | 0，，01： | 1 |
| 7 | Damping capacity in Fe -Mn binary alloys显示文摘 | Lee Y K Jun J H Choi C S | 1997 | ISIJ International1997，37，10： | 1 |
| 8 | Optimization of pigment production of Monascus purpureus P-57 in liquid culture显示文摘 | DUCK P C JUN J H YU T S | 2005 | Korean Society for Biotechnology and Bioengineering2005，20，1： | 1 |
| 9 | DNA Polymerase and Topoisomerase II Inhibitors from Psoralea corylifalia 显示文摘 | Jun Sun N S H W And J M C | 1998 | Journal of Natural Products1998，61，3： | 1 |
| 10 | An RF-MEMS switch with low-actuation voltage and high reliability 显示文摘 | LEE S D JUN B C RHEE J K | 2006 | Journal of Microelectromechanicat Systems2006，15，6： | 1 |
| 11 | Damping capacity in Fe-Mn binary alloys显示文摘 | Lee Y K Jun J H Choi C S | 1997 | ISIJ International1997，37，10： | 1 |
| 12 | Methylation of Naph- thalene with Methanol over Beta, Mordernite, ZSM-12 and MCM-22 Zeolite Catalysts 显示文摘 | Park J N Wang Jun Lee C W | 2002 | Bull Korean Chem Soc2002，23，7： | 1 |
| 13 | Antihypertensiveeffect and safety evaluation of vegetable drink with peptides derivedfrom sardine protein hydrolysates on mild hypertensive, high-normaland normal blood pressure subjects显示文摘 | KAWASAKI T JUN C J FUKUSHINA Y | 2002 | Fukuoka Igaku Zasshi2002，93，10： | 1 |
| 14 | Electrorheological character- istics of poly ( o - ethoxy ) aniline nanoeomposite 显示文摘 | JUN H S HYOUNG J C | 2002 | Joumai of Electroanalytical Chemistry2002，518，： | 1 |
| 15 | Fuzzy soft set theory applied to BCK/BCI algebras显示文摘 | Jun Y B Lee K J Park C H | 2010 | Computers and Mathematics with Applications2010，59，： | 1 |
| 16 | Mesenchymal stem cells derived from human adipose tissues favor tumor cell growth in vivo显示文摘 | Yu J M Jun E S Bae Y C | 2008 | stem Cells Dev2008，17，3： | 1 |
| 17 | Damping mechanisms of Fe-Mn alloy with (γ+ε) dual phase structure 显示文摘 | J H Jun Y K Lee C S Choi | 2000 | Materials Science and Technology2000，16，： | 1 |
| 18 | Methanol Conversion on SAPO-34 Catalysts Prepared by Mixed Template Method 显示文摘 | Lee Y J Beak S C Jun K W | 2007 | Appl Catal A : General2007，329，1： | 1 |
| 19 | Comparative study of YBCO film growth in the cold wall type and hot wall type MOCVD processes 显示文摘 | Kim C J Jun B H Kim H J et ell | 2005 | Physica C : Superconductivity and its Applications2005，，： | 1 |
| 20 | Thermal con-ductivity of single-crystalline MgB2显示文摘 | Jun J Kazakov S M | 2002 | Phys Rev2002，66，1： | 1 |
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