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| 1 | An experimental study of flow and heat transfer in sinusoidal wavy passages显示文摘 | Rush T A Newell T A Jacobi A M | 1999 | Int J Heat Mass Transfer1999，42，9： | 2 |
| 2 | The role of computational methods in the identification of bioactive compounds显示文摘 | Glick M Jacoby E | 2011 | Curr Opin Chem Biol2011，15，4： | 1 |
| 3 | INPP5E mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in human and mouse 显示文摘 | Jacoby M Cox JJ Gayral S | 2009 | Nat Genet2009，41，9： | 1 |
| 4 | INPPSE mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in human and mouse显示文摘 | Jacoby M Cox JJ Gayral S | 2009 | Nat Genet2009，41，9： | 1 |
| 5 | Agroecosystem resilience and farmer' perceptions of climate change impacts on cocoa farms in Alto Beni, Bolivia显示文摘 | Jacobi J Schneider M Bottazzi P | 2013 | Renewable Agriculture and Food Systems2013，30，： | 1 |
| 6 | Assessment of tobacco heating system 2.4 on osteogenic differentiation of mesenchymal stem cells and primary human osteoblasts compared to conventional cigarettes显示文摘BACKGROUND Cigarette smoking(CS)is the most common method of consuming tobacco.Deleterious effects on bone integrity,increased incidence of fractures,and delayed fracture healing are all associated with CS.Over 150 of the 6500 molecular species contained in cigarette smoke and identified as toxic compounds are inhaled by CS and,via the bloodstream,reach the skeletal system.New technologies designed to develop a reduced-risk alternative for smokers are based on electronic nicotine delivery systems,such as e-cigarettes and tobacco heating systems(THS).THS are designed to heat tobacco instead of burning it,thereby reducing the levels of harmful toxic compounds released.AIM To examine the effects of THS on osteoprogenitor cell viability and function compared to conventional CS.METHODS Human immortalized mesenchymal stem cells(n=3)and primary human preosteoblasts isolated from cancellous bone samples from BG Unfall Klinik Tübingen(n=5)were osteogenically differentiated in vitro with aqueous extracts generated from either the THS 2.4“IQOS”or conventional“Marlboro”cigarettes for up to 21 d.Cell viability was analyzed using resazurin conversion assay(mitochondrial activity)and calcein-AM staining(esterase activity).Osteogenic differentiation and bone cell function were evaluated using alkaline phosphatase(AP)activity,while matrix formation was analyzed through alizarin red staining.Primary cilia structure was examined by acetylatedα-tubulin immunofluorescent staining.Free radical production was evaluated with 2’,7’-dichlorofluoresceindiacetate assay.RESULTS Our data clearly show that THS is significantly less toxic to bone cells than CS when analyzed by mitochondrial and esterase activity(P<0.001).No significant differences in cytotoxicity between the diverse flavors of THS were observed.Harmful effects from THS on bone cell function were observed only at very high,non-physiological concentrations.In contrast,extracts from conventional cigarettes significantly reduced the AP activity(by two-fold)and matrix mineralization(four-fold)at low concentrations.Additionally,morphologic analysis of primary cilia revealed no significant changes in the length of the organelle involved in osteogenesis of osteoprogenitor cells,nor in the number of ciliated cells following THS treatment.Assessment of free radical production demonstrated that THS induced significantly less oxidative stress than conventional CS in osteoprogenitor cells.CONCLUSIONTHS was significantly less harmful to osteoprogenitor cells during osteogenesisthan conventional CS. Additional studies are required to confirm whether THS isa better alternative for smokers to improve delays in bone healing followingfracture. | Romina H Aspera-Werz Sabrina Ehnert Monja Müller Sheng Zhu Tao Chen Weidong Weng Johann Jacoby Andreas K Nussler | 2020 | World Journal of Stem Cells2020，12，8： | 1 |
| 7 | Emerging plasmid-mediated quinolone resistance associated with the qnr gene in Klebsiella pneumoniae clinical isolates in the United States 显示文摘 | Wang M G Sahm D F Jacoby G A | 2004 | Antimicrob Agents Chemother2004，48，4： | 1 |
| 8 | Direct mass spectrometric studies of the destructionof harzardous wastes显示文摘 | Jacoby W A Nimlos N R Blake D M | 1993 | Envion Sci Technol1993，27，： | 1 |
| 9 | Adjuvant postop- erative radiation therapy after curative resection of squamous cell carcinoma of the thoracic esophagus: a prospective ran- domized study显示文摘 | Zieren H U Muller J M Jacobi C A | 1995 | World J Surg1995，19，3： | 1 |
| 10 | Doublestranded RNA causes airway hyperreactivity and ne/tronal M2 muscarinic receptor dysfunction 显示文摘 | Bowerfind W M L Fryer A D Jacoby D B | 2002 | J Appl Physiol2002，92，： | 1 |
| 11 | Part I:presentation of the model显示文摘 | Thome J R Dupont V Jacobi A M Heat transfer model for evaporation in microchannels | 2004 | Int J Heat Mass Transfer2004，47，1416： | 1 |
| 12 | Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai,China显示文摘 | Wang M G Tran J H Jacoby G A | 2003 | Antimicrob Agents Chemother2003，47，7： | 1 |
| 13 | Plasmid mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China显示文摘 | Wang M Tran J H Jacoby G A | 2003 | Antimicrob Agents Chemother2003，47，7： | 1 |
| 14 | Air-side flow and heat transfer in compact heat exchangers: a discussion of enhancement mechanisms显示文摘 | Jacobi A M Shah R K | 1998 | Heat Transfer Engineering1998，19，4： | 1 |
| 15 | Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai,China显示文摘 | Wang M Tran JH Jacoby GA | 2003 | Antimicrob Agents Chemother2003，47，7： | 1 |
| 16 | Molecular analysis of the NF2 tumor-suppressor gene in schwannomatosis显示文摘 | Jacoby L B Jones D Davis K Kronn D Short M P Gusella J | 1997 | Am J Hum Genet1997，61，： | 1 |
| 17 | Effects of microgroove geometry on the early stages of frost formation and frost properties显示文摘 | Rahman M A Jacobi A M | 2013 | Applied Thermal Engineering2013，56，12： | 1 |
| 18 | Magnetic resonance imaging of benign cardiac masses: a pictorial essay显示文摘 | Ward T J Kadoch M A Jacobi A H | 2013 | Clin Imaging Sei2013，3，： | 1 |
| 19 | qnrB,another plasmid-mediated gene for quionlone resistanee显示文摘 | Jacoby G A Walsh K E Mills D M | 2006 | Antimierob Agents Chemother2006，50，4： | 1 |
| 20 | Quinolo- ne resistance from a transferable plasmid 显示文摘 | MARTINEZ M L PASCUAL A JACOBY G A | 1998 | Lancet1998，351，： | 1 |
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